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imagelab analytic software  (Bio-Rad)


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    Bio-Rad imagelab analytic software
    Imagelab Analytic Software, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 35737 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/analytical+imaging+software/Image+Lab+Software/pmc12513116-185-0-4
    Average 99 stars, based on 35737 article reviews
    imagelab analytic software - by Bioz Stars, 2026-11
    99/100 stars

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    other:

    Article Title: Exercise attenuates stress-related signaling as sensed by higher phosphorylation of small heat shock proteins in skeletal muscle from older individuals
    Article Snippet: After washing and incubating with an appropriate horse radish peroxidase (HRP) secondary antibody (HRP goat anti-mouse 51782504 and HRP goat anti-rabbit 17065156; Bio-Rad Laboratories) and following TBST washes, the membranes were coated with chemiluminescent substrate (West Femto; Thermo Fisher Scientific).

    Article Title: Modified tumor uptake and biodistribution of nanoparticles coated with small extracellular vesicle membranes derived from distinct tumor cell lines
    Article Snippet: After washing, protein bands were visualized using SuperSignal West Femto (34096, ThermoFisher) and imaged on ChemiDoc MP imaging system (Bio-Rad).

    Software:

    Article Title: CASTOR1 functional inactivation drives KRAS -mutant NSCLC and therapy resistance by a noncanonical mechanism of tumor suppression
    Article Snippet: The primary antibodies used in the analysis included the following: anti-CASTOR1 (Abclonal, catalog no. A2071C), anti-AKT (Cell Signaling Technology, catalog no. 4691, RRID:AB_915783), anti–p-AKT–S473 (Cell Signaling Technology, catalog no. 4060), anti–p-AKT–T308 (Cell Signaling Technology, catalog no. 4056, RRID:AB_331163), anti-S6K (Cell Signaling Technology, catalog no. 97596, RRID:AB_2800283), anti-4EBP1 (Cell Signaling Technology, catalog no. 9644, RRID:AB_2097841), anti–p-4EBP1 (Cell Signaling Technology, catalog no. 2855), anti-S6 (Cell Signaling Technology, catalog no. 2217, RRID:AB_331355), anti–p-S6 (Cell Signaling Technology, catalog no. 2211), anti–p-ERK (Cell Signaling Technology, catalog no. 4370), anti-RAS (Cell Signaling Technology, catalog no. 91054), anti-KRAS G12D (Cell Signaling Technology, catalog no. 14429, RRID:AB_2728748), and anti-GAPDH (Cell Signaling Technology, catalog no. 5174, RRID:AB_10622025), all of which were used at 1:1000 dilution. .. Band intensities were quantified using Image Lab software (Bio-Rad). ..

    Article Title: Curcumin-Induced Vacuolizing Stress Reveals a Vulnerability Associated with NF1 Loss in Schwann Cells.
    Article Snippet: Membranes were probed with the following antibodies: anti-NF1 #sc-376886 (1:200) by Santa Cruz Biotechnology (Dallas, TX, USA), anti-PARP #10872500 (1:1000) by Roche, anti-NRF2 #PA58804 (1:1000) by Life Technologies, anti-TRX #EPR6110 (1:1000) by Abcam (Cambridge, UK), anti-SOD1 #TA50495 (1:1000) by OriGene Technologies, (Rockville, MD, USA), anti-cleaved caspase-3 (Asp175) #9661 (1:1000) by Cell Signaling Technology (Danvers, MA, USA) and anti-GAPDH-HRP #MA5-15738-HRP (1:5000) by Life Technologies. .. Bound antibodies were detected by chemiluminescence using WesternBright ECL (#K12045D50 Advansta, San Jose, CA, USA), and band intensities were quantified by densitometry with Image Lab software 5.0 (Bio-Rad, Hercules, CA, USA). ..

    Article Title: CCAN forms the spherical shell-like structure in interphase kinetochores.
    Article Snippet: The supernatant was then boiled at 96°C for 5 min. For SDS–polyacrylamide gel electrophoresis (PAGE) analysis, 25 pmol of each protein sample and 10 μl of the supernatant were loaded as the input and pull- down samples, respectively, on a 10–20% SuperSep Ace gel (Wako, catalog no. 191- 15031). .. After visualizing the protein bands by CBB staining, gel images were acquired using the ChemiDoc Touch MP imaging system (Bio- Rad) and analyzed using the Image Lab software v6.1.0. .. For quantitative analysis, the band intensity of pulled down CENP- N was normalized by that of GST–Mini- CENP- C. Data are shown as mean ± SD from three technical replicates and generated using GraphPad Prism v9.5.1.

    Article Title: Influence of diet-induced obesity and voluntary exercise training on cardiac lipids and mitochondrial function in mice
    Article Snippet: Blots were covered in Clarity Max Chemiluminescent reagent (Bio-Rad) and visualized using the ChemiDoc XRS+ Imaging System (Bio-Rad). .. Densitometry was analyzed using ImageLab software (V6.0.1; Bio-Rad). .. Immunoblotting was performed with antibodies against lysocardiolipin acyltransferase 1(LCLAT1, PA5-25627; Thermo Fisher Scientific), PGC-1α (ab191838; Abcam, Cambridge, UK), MFN2 (PA5-118059; Thermo Fisher Scientific), ANP (sc-18811; Santa Cruz, Dallas, TX, USA), tumor necrosis factor alpha (TNFα, 3707; Cell Signaling, Danvers, MA, USA), phosphorylated adenosine monophosphate-activated protein kinase (AMPK, 2531; Cell Signaling), AMPKα (2532; Cell Signaling), Perilipin 5 (PA1-46215; Thermo Fisher Scientific), translocase of outer mitochondria membrane 70 (TOM70, 65675; Cell Signaling), cytochrome c oxidase subunit 4 (COXIV, 4844; Cell Signaling), and calnexin (208880; Merck, Rahway, NJ, USA).

    Article Title: Interleukin‐35 Ameliorates Obesity‐Associated Impairment of Endothelium‐Dependent Vasodilation via the STAT3‐MAPK/ERK‐eNOS Signaling Axis
    Article Snippet: .. Following incubation with HRP‐conjugated goat anti‐rabbit IgG and goat anti‐mouse IgG secondary antibodies (both 1:5000), protein bands were analyzed using Image Lab 6.0 software (BioRad, USA). ..

    Article Title: Exercise attenuates stress-related signaling as sensed by higher phosphorylation of small heat shock proteins in skeletal muscle from older individuals
    Article Snippet: After a series of steps involving drying, re-activating, washing, and blocking, membranes were incubated in antibodies (for full details, see Latchman et al., ): firstly, anti-myosin heavy chain IIa (MHCIIa) (mouse, monoclonal IgG, A4.74; Developmental Studies Hybridoma Bank (DSHB), Iowa city, IA, USA), followed by stripping and then anti-MHCI (mouse monoclonal IgM, A4.840; DSHB). .. Chemiluminescent substrate (ECL, Clarity; Bio-Rad Laboratories, Sydney, Australia) enabled imaging of dots using a Chemidoc MP and Image Lab software (Bio-Rad Laboratories). .. Chemiluminescent substrate (ECL, Clarity; Bio-Rad Laboratories, Sydney, Australia) enabled imaging of dots using a Chemidoc MP and Image Lab software (Bio-Rad Laboratories).

    Staining:

    Article Title: CCAN forms the spherical shell-like structure in interphase kinetochores.
    Article Snippet: The supernatant was then boiled at 96°C for 5 min. For SDS–polyacrylamide gel electrophoresis (PAGE) analysis, 25 pmol of each protein sample and 10 μl of the supernatant were loaded as the input and pull- down samples, respectively, on a 10–20% SuperSep Ace gel (Wako, catalog no. 191- 15031). .. After visualizing the protein bands by CBB staining, gel images were acquired using the ChemiDoc Touch MP imaging system (Bio- Rad) and analyzed using the Image Lab software v6.1.0. .. For quantitative analysis, the band intensity of pulled down CENP- N was normalized by that of GST–Mini- CENP- C. Data are shown as mean ± SD from three technical replicates and generated using GraphPad Prism v9.5.1.

    Imaging:

    Article Title: CCAN forms the spherical shell-like structure in interphase kinetochores.
    Article Snippet: The supernatant was then boiled at 96°C for 5 min. For SDS–polyacrylamide gel electrophoresis (PAGE) analysis, 25 pmol of each protein sample and 10 μl of the supernatant were loaded as the input and pull- down samples, respectively, on a 10–20% SuperSep Ace gel (Wako, catalog no. 191- 15031). .. After visualizing the protein bands by CBB staining, gel images were acquired using the ChemiDoc Touch MP imaging system (Bio- Rad) and analyzed using the Image Lab software v6.1.0. .. For quantitative analysis, the band intensity of pulled down CENP- N was normalized by that of GST–Mini- CENP- C. Data are shown as mean ± SD from three technical replicates and generated using GraphPad Prism v9.5.1.

    Article Title: Exercise attenuates stress-related signaling as sensed by higher phosphorylation of small heat shock proteins in skeletal muscle from older individuals
    Article Snippet: After a series of steps involving drying, re-activating, washing, and blocking, membranes were incubated in antibodies (for full details, see Latchman et al., ): firstly, anti-myosin heavy chain IIa (MHCIIa) (mouse, monoclonal IgG, A4.74; Developmental Studies Hybridoma Bank (DSHB), Iowa city, IA, USA), followed by stripping and then anti-MHCI (mouse monoclonal IgM, A4.840; DSHB). .. Chemiluminescent substrate (ECL, Clarity; Bio-Rad Laboratories, Sydney, Australia) enabled imaging of dots using a Chemidoc MP and Image Lab software (Bio-Rad Laboratories). .. Chemiluminescent substrate (ECL, Clarity; Bio-Rad Laboratories, Sydney, Australia) enabled imaging of dots using a Chemidoc MP and Image Lab software (Bio-Rad Laboratories).

    Incubation:

    Article Title: Interleukin‐35 Ameliorates Obesity‐Associated Impairment of Endothelium‐Dependent Vasodilation via the STAT3‐MAPK/ERK‐eNOS Signaling Axis
    Article Snippet: .. Following incubation with HRP‐conjugated goat anti‐rabbit IgG and goat anti‐mouse IgG secondary antibodies (both 1:5000), protein bands were analyzed using Image Lab 6.0 software (BioRad, USA). ..



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    Gene expression analysis of OCLN and markers related to its trafficking in WT and CB1 −/− (KO) testes. RTqPCR analysis of Ocln mRNA. Transcript amounts were reported as normalized fold expression (nfe) relatively to Rps18 gene (A). Western blot analysis of OCLN. Protein amounts were quantified by densitometry analysis, normalized against ERK1/2 signals and expressed in OD values (B). Immunofluorescence analysis of OCLN; white arrowheads represent OCLN localization (green) in testis. Nuclei were labelled with DAPI (blue). Scale bar: 5 µm (C). Quantitative analysis of OCLN green fluorescence on supra‐basal epithelium Regions of Interest (ROI); values are expressed as Sum of Intensity/area (SUM (I)/pixel 2 ), measured using the <t>Nikon</t> <t>Imaging</t> <t>Analytical</t> <t>Software</t> (NIS‐Elements Analysis D 4.40.00, 64 bit) (D). RTqPCR analysis of endocytic (Eea1), recycling (Rab13) and degradation (Rab9 and Itch) markers of OCLN. Transcript amounts were reported as normalized fold expression (nfe) relatively to Rps18 gene (E). All data were reported as mean value ± S.E.M. (* p < 0.05; ** p < 0.01).
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    Gene expression analysis of OCLN and markers related to its trafficking in WT and CB1 −/− (KO) testes. RTqPCR analysis of Ocln mRNA. Transcript amounts were reported as normalized fold expression (nfe) relatively to Rps18 gene (A). Western blot analysis of OCLN. Protein amounts were quantified by densitometry analysis, normalized against ERK1/2 signals and expressed in OD values (B). Immunofluorescence analysis of OCLN; white arrowheads represent OCLN localization (green) in testis. Nuclei were labelled with DAPI (blue). Scale bar: 5 µm (C). Quantitative analysis of OCLN green fluorescence on supra‐basal epithelium Regions of Interest (ROI); values are expressed as Sum of Intensity/area (SUM (I)/pixel 2 ), measured using the <t>Nikon</t> <t>Imaging</t> <t>Analytical</t> <t>Software</t> (NIS‐Elements Analysis D 4.40.00, 64 bit) (D). RTqPCR analysis of endocytic (Eea1), recycling (Rab13) and degradation (Rab9 and Itch) markers of OCLN. Transcript amounts were reported as normalized fold expression (nfe) relatively to Rps18 gene (E). All data were reported as mean value ± S.E.M. (* p < 0.05; ** p < 0.01).
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    Image Search Results


    Gene expression analysis of OCLN and markers related to its trafficking in WT and CB1 −/− (KO) testes. RTqPCR analysis of Ocln mRNA. Transcript amounts were reported as normalized fold expression (nfe) relatively to Rps18 gene (A). Western blot analysis of OCLN. Protein amounts were quantified by densitometry analysis, normalized against ERK1/2 signals and expressed in OD values (B). Immunofluorescence analysis of OCLN; white arrowheads represent OCLN localization (green) in testis. Nuclei were labelled with DAPI (blue). Scale bar: 5 µm (C). Quantitative analysis of OCLN green fluorescence on supra‐basal epithelium Regions of Interest (ROI); values are expressed as Sum of Intensity/area (SUM (I)/pixel 2 ), measured using the Nikon Imaging Analytical Software (NIS‐Elements Analysis D 4.40.00, 64 bit) (D). RTqPCR analysis of endocytic (Eea1), recycling (Rab13) and degradation (Rab9 and Itch) markers of OCLN. Transcript amounts were reported as normalized fold expression (nfe) relatively to Rps18 gene (E). All data were reported as mean value ± S.E.M. (* p < 0.05; ** p < 0.01).

    Journal: Journal of Cellular Physiology

    Article Title: Cannabinoid Receptors Modulate Physiological Remodelling of the Blood–Testis Barrier

    doi: 10.1002/jcp.70109

    Figure Lengend Snippet: Gene expression analysis of OCLN and markers related to its trafficking in WT and CB1 −/− (KO) testes. RTqPCR analysis of Ocln mRNA. Transcript amounts were reported as normalized fold expression (nfe) relatively to Rps18 gene (A). Western blot analysis of OCLN. Protein amounts were quantified by densitometry analysis, normalized against ERK1/2 signals and expressed in OD values (B). Immunofluorescence analysis of OCLN; white arrowheads represent OCLN localization (green) in testis. Nuclei were labelled with DAPI (blue). Scale bar: 5 µm (C). Quantitative analysis of OCLN green fluorescence on supra‐basal epithelium Regions of Interest (ROI); values are expressed as Sum of Intensity/area (SUM (I)/pixel 2 ), measured using the Nikon Imaging Analytical Software (NIS‐Elements Analysis D 4.40.00, 64 bit) (D). RTqPCR analysis of endocytic (Eea1), recycling (Rab13) and degradation (Rab9 and Itch) markers of OCLN. Transcript amounts were reported as normalized fold expression (nfe) relatively to Rps18 gene (E). All data were reported as mean value ± S.E.M. (* p < 0.05; ** p < 0.01).

    Article Snippet: The green fluorescence signal diffused in the luminal compartment was quantified using the Nikon Imaging Analytical Software (NIS‐Elements Analysis D 4.40.00, 64 bit) (see Figure ).

    Techniques: Gene Expression, Expressing, Western Blot, Immunofluorescence, Fluorescence, Imaging, Software

    Responsiveness of OCLN expression to CB1 and CB2 receptors. Western blot analysis of OCLN in testis of HT mice in vitro treated with vehicle (CTRL), ACEA (A) or JWH‐133 (B) at different concentrations: 0.1–1–10 µM. Protein amounts were quantified by densitometry analysis, normalized against ERK1/2 signals and expressed in OD as fold change. Immunofluorescence analysis of OCLN in testis of CTRL and ACEA (10 µM) or JWH‐133 (1 µM); white arrowheads represent OCLN localization (green) in testis. Nuclei were labelled with DAPI (blue). Scale bar: 5 µm (C). Quantitative analysis of OCLN green fluorescence on supra‐basal epithelium Regions of Interest (ROI); values are expressed as Sum of Intensity/area (SUM (I)/pixel 2 ), measured using the Nikon Imaging Analytical Software (NIS‐Elements Analysis D 4.40.00, 64 bit) (D). RTqPCR analysis of Ocln mRNA in testis of CTRL and ACEA 10 µM (E) or JWH‐133 1 µM (F). Transcript amounts were reported as normalized fold expression (nfe) relatively to Rps18 gene. All the data were reported as mean value ± S.E.M. (* p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001; a vs. b: p < 0.05).

    Journal: Journal of Cellular Physiology

    Article Title: Cannabinoid Receptors Modulate Physiological Remodelling of the Blood–Testis Barrier

    doi: 10.1002/jcp.70109

    Figure Lengend Snippet: Responsiveness of OCLN expression to CB1 and CB2 receptors. Western blot analysis of OCLN in testis of HT mice in vitro treated with vehicle (CTRL), ACEA (A) or JWH‐133 (B) at different concentrations: 0.1–1–10 µM. Protein amounts were quantified by densitometry analysis, normalized against ERK1/2 signals and expressed in OD as fold change. Immunofluorescence analysis of OCLN in testis of CTRL and ACEA (10 µM) or JWH‐133 (1 µM); white arrowheads represent OCLN localization (green) in testis. Nuclei were labelled with DAPI (blue). Scale bar: 5 µm (C). Quantitative analysis of OCLN green fluorescence on supra‐basal epithelium Regions of Interest (ROI); values are expressed as Sum of Intensity/area (SUM (I)/pixel 2 ), measured using the Nikon Imaging Analytical Software (NIS‐Elements Analysis D 4.40.00, 64 bit) (D). RTqPCR analysis of Ocln mRNA in testis of CTRL and ACEA 10 µM (E) or JWH‐133 1 µM (F). Transcript amounts were reported as normalized fold expression (nfe) relatively to Rps18 gene. All the data were reported as mean value ± S.E.M. (* p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001; a vs. b: p < 0.05).

    Article Snippet: The green fluorescence signal diffused in the luminal compartment was quantified using the Nikon Imaging Analytical Software (NIS‐Elements Analysis D 4.40.00, 64 bit) (see Figure ).

    Techniques: Expressing, Western Blot, In Vitro, Immunofluorescence, Fluorescence, Imaging, Software

    CB1 and CB2 action on BTB permeability and functionality. FITC‐dextran diffusion assay in testis of WT and HT mice in vitro treated with vehicle (CTRL), ACEA 10 µM and JWH‐133 1 µM (A) Scale bar: 300 μm. The green fluorescence signal diffused in the luminal compartment was quantified using the Nikon Imaging Analytical Software (NIS‐Elements Analysis D 4.40.00, 64 bit) as SUM (I)/pixel 2 (see also Figure for technical details) (B) a versus b: p < 0.05. RTqPCR analysis of endocytic (Eea1), recycling (Rab13) and degradation (Rab9 and Itch) markers of OCLN in the testis of CTRL and ACEA 10 µM (C) or JWH‐133 1 µM (D). Transcript amounts were reported as normalized fold expression (nfe) relatively to Rps18 gene. All data were reported as mean value ± S.E.M. (* p < 0.05; ** p < 0.01).

    Journal: Journal of Cellular Physiology

    Article Title: Cannabinoid Receptors Modulate Physiological Remodelling of the Blood–Testis Barrier

    doi: 10.1002/jcp.70109

    Figure Lengend Snippet: CB1 and CB2 action on BTB permeability and functionality. FITC‐dextran diffusion assay in testis of WT and HT mice in vitro treated with vehicle (CTRL), ACEA 10 µM and JWH‐133 1 µM (A) Scale bar: 300 μm. The green fluorescence signal diffused in the luminal compartment was quantified using the Nikon Imaging Analytical Software (NIS‐Elements Analysis D 4.40.00, 64 bit) as SUM (I)/pixel 2 (see also Figure for technical details) (B) a versus b: p < 0.05. RTqPCR analysis of endocytic (Eea1), recycling (Rab13) and degradation (Rab9 and Itch) markers of OCLN in the testis of CTRL and ACEA 10 µM (C) or JWH‐133 1 µM (D). Transcript amounts were reported as normalized fold expression (nfe) relatively to Rps18 gene. All data were reported as mean value ± S.E.M. (* p < 0.05; ** p < 0.01).

    Article Snippet: The green fluorescence signal diffused in the luminal compartment was quantified using the Nikon Imaging Analytical Software (NIS‐Elements Analysis D 4.40.00, 64 bit) (see Figure ).

    Techniques: Permeability, Diffusion-based Assay, In Vitro, Fluorescence, Imaging, Software, Expressing