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algorithms imagelab analytic software biorad proteome discoverer software  (Bio-Rad)


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    Bio-Rad algorithms imagelab analytic software biorad proteome discoverer software
    Algorithms Imagelab Analytic Software Biorad Proteome Discoverer Software, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 35689 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/analytical+imaging+software/Image+Lab+Software/pm41079607-296-7-11
    Average 99 stars, based on 35689 article reviews
    algorithms imagelab analytic software biorad proteome discoverer software - by Bioz Stars, 2026-10
    99/100 stars

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    other:

    Article Title: Exercise attenuates stress-related signaling as sensed by higher phosphorylation of small heat shock proteins in skeletal muscle from older individuals
    Article Snippet: After washing and incubating with an appropriate horse radish peroxidase (HRP) secondary antibody (HRP goat anti-mouse 51782504 and HRP goat anti-rabbit 17065156; Bio-Rad Laboratories) and following TBST washes, the membranes were coated with chemiluminescent substrate (West Femto; Thermo Fisher Scientific).

    Article Title: Modified tumor uptake and biodistribution of nanoparticles coated with small extracellular vesicle membranes derived from distinct tumor cell lines
    Article Snippet: After washing, protein bands were visualized using SuperSignal West Femto (34096, ThermoFisher) and imaged on ChemiDoc MP imaging system (Bio-Rad).

    Alternative Splicing:

    Article Title: Alternative splicing of a species- and skeletal muscle-specific microexon diversifies the menu of clathrin heavy chains in mice and rats.
    Article Snippet: PCR products were resolved via 6% polyacrylamide gel electrophoresis using TAE buffer (40 mM Tris, 20 mM acetic acid, 1 mM EDTA pH 8.5) for 3–4 h at 150–160 V. Polyacrylamide gels were stained with 0.4 μg/mL ethidium bromide for 10 min. All gels were imaged under ultraviolet illumination in a ChemiDoc XRS+ Imaging System (Bio-Rad). .. Alternative splicing was quantified by densitometry using the Image Lab software (version 6.0.1, Bio-Rad). .. The product length-dependent staining bias of ethidium bromide was corrected using a standard curve based upon the pUC19 ladder (Thermo Fisher Scientific, SM0221).

    Software:

    Article Title: Alternative splicing of a species- and skeletal muscle-specific microexon diversifies the menu of clathrin heavy chains in mice and rats.
    Article Snippet: PCR products were resolved via 6% polyacrylamide gel electrophoresis using TAE buffer (40 mM Tris, 20 mM acetic acid, 1 mM EDTA pH 8.5) for 3–4 h at 150–160 V. Polyacrylamide gels were stained with 0.4 μg/mL ethidium bromide for 10 min. All gels were imaged under ultraviolet illumination in a ChemiDoc XRS+ Imaging System (Bio-Rad). .. Alternative splicing was quantified by densitometry using the Image Lab software (version 6.0.1, Bio-Rad). .. The product length-dependent staining bias of ethidium bromide was corrected using a standard curve based upon the pUC19 ladder (Thermo Fisher Scientific, SM0221).

    Article Title: Single-cell RNA sequencing identifies a proinflammatory S100a8/a9 + macrophage subset contributing to acute kidney injury.
    Article Snippet: 1 Department of Nephrology, Zhongshan Hospital, Fudan University, Shanghai, China 2 Kidney and Blood Purification Laboratory of Shanghai, Shanghai, China 3 Shanghai Medical Center of Kidney, Shanghai, China 4 Kidney and Dialysis Institute of Shanghai, Shanghai, China 5 Hemodialysis Quality Control Center of Shanghai, Shanghai, China Abstract Objective Macrophages represent a heterogeneous cell population in the kidney.. However, macrophage subsets and their role in endotoxemia-induced acute kidney injury (AKI) remain elucidated.. Material and methods Single-cell RNA sequencing was performed to identify macrophage subpopulations in mouse kidney following lipopolysaccharide (LPS) challenge.

    Article Title: Influence of diet-induced obesity and voluntary exercise training on cardiac lipids and mitochondrial function in mice
    Article Snippet: Blots were covered in Clarity Max Chemiluminescent reagent (Bio-Rad) and visualized using the ChemiDoc XRS+ Imaging System (Bio-Rad). .. Densitometry was analyzed using ImageLab software (V6.0.1; Bio-Rad). .. Immunoblotting was performed with antibodies against lysocardiolipin acyltransferase 1(LCLAT1, PA5-25627; Thermo Fisher Scientific), PGC-1α (ab191838; Abcam, Cambridge, UK), MFN2 (PA5-118059; Thermo Fisher Scientific), ANP (sc-18811; Santa Cruz, Dallas, TX, USA), tumor necrosis factor alpha (TNFα, 3707; Cell Signaling, Danvers, MA, USA), phosphorylated adenosine monophosphate-activated protein kinase (AMPK, 2531; Cell Signaling), AMPKα (2532; Cell Signaling), Perilipin 5 (PA1-46215; Thermo Fisher Scientific), translocase of outer mitochondria membrane 70 (TOM70, 65675; Cell Signaling), cytochrome c oxidase subunit 4 (COXIV, 4844; Cell Signaling), and calnexin (208880; Merck, Rahway, NJ, USA).

    Article Title: Targeting activated hepatic stellate cells through ferroptosis: phenethyl isothiocyanate attenuates CCl 4 -induced liver fibrosis by regulating iron metabolism, redox homeostasis, and autophagy.
    Article Snippet: Phenethyl isothiocyanate (PEITC), a dietary compound from cruciferous vegetables, is known for its chemopreventive activity, but its effects on liver fibrosis remain largely unexplored.. In this study, we show that PEITC administration attenuated carbon tetrachloride (CCl4)-induced hepatic injury and fibrosis in mice, as evidenced by reduced collagen deposition, hydroxyproline content and fibrotic marker expression, and improved liver function.. PEITC inhibited cell survival and fibrogenic phenotypes in transforming growth factor β (TGF-β)-stimulated LX-2 human hepatic stellate cells (HSCs) while exhibiting minimal cytotoxicity towards normal hepatocytes.

    Article Title: Exercise attenuates stress-related signaling as sensed by higher phosphorylation of small heat shock proteins in skeletal muscle from older individuals
    Article Snippet: After a series of steps involving drying, re-activating, washing, and blocking, membranes were incubated in antibodies (for full details, see Latchman et al., ): firstly, anti-myosin heavy chain IIa (MHCIIa) (mouse, monoclonal IgG, A4.74; Developmental Studies Hybridoma Bank (DSHB), Iowa city, IA, USA), followed by stripping and then anti-MHCI (mouse monoclonal IgM, A4.840; DSHB). .. Chemiluminescent substrate (ECL, Clarity; Bio-Rad Laboratories, Sydney, Australia) enabled imaging of dots using a Chemidoc MP and Image Lab software (Bio-Rad Laboratories). .. Chemiluminescent substrate (ECL, Clarity; Bio-Rad Laboratories, Sydney, Australia) enabled imaging of dots using a Chemidoc MP and Image Lab software (Bio-Rad Laboratories).

    Article Title: OGT regulates RNA-binding protein nucleocytoplasmic partitioning, but this fails in amyotrophic lateral sclerosis.
    Article Snippet: All membranes were imaged using a ChemiDoc MP imaging system (Bio-Rad). .. Band intensities were analyzed using Image Lab software (Bio-Rad). .. Samples were fixed in 4% paraformaldehyde (Thermo Scientific, 043368.9M) diluted in PBS (Gibco, 10010015) and then blocked in 5% bovine serum albumin (BSA, Sigma-Aldrich, A7906) in PBS, containing 0.3% Triton X-100 (Thermo Scientific, 9002–93-1).

    Incubation:

    Article Title: Targeting activated hepatic stellate cells through ferroptosis: phenethyl isothiocyanate attenuates CCl 4 -induced liver fibrosis by regulating iron metabolism, redox homeostasis, and autophagy.
    Article Snippet: Phenethyl isothiocyanate (PEITC), a dietary compound from cruciferous vegetables, is known for its chemopreventive activity, but its effects on liver fibrosis remain largely unexplored.. In this study, we show that PEITC administration attenuated carbon tetrachloride (CCl4)-induced hepatic injury and fibrosis in mice, as evidenced by reduced collagen deposition, hydroxyproline content and fibrotic marker expression, and improved liver function.. PEITC inhibited cell survival and fibrogenic phenotypes in transforming growth factor β (TGF-β)-stimulated LX-2 human hepatic stellate cells (HSCs) while exhibiting minimal cytotoxicity towards normal hepatocytes.

    Automatic:

    Article Title: Targeting activated hepatic stellate cells through ferroptosis: phenethyl isothiocyanate attenuates CCl 4 -induced liver fibrosis by regulating iron metabolism, redox homeostasis, and autophagy.
    Article Snippet: Phenethyl isothiocyanate (PEITC), a dietary compound from cruciferous vegetables, is known for its chemopreventive activity, but its effects on liver fibrosis remain largely unexplored.. In this study, we show that PEITC administration attenuated carbon tetrachloride (CCl4)-induced hepatic injury and fibrosis in mice, as evidenced by reduced collagen deposition, hydroxyproline content and fibrotic marker expression, and improved liver function.. PEITC inhibited cell survival and fibrogenic phenotypes in transforming growth factor β (TGF-β)-stimulated LX-2 human hepatic stellate cells (HSCs) while exhibiting minimal cytotoxicity towards normal hepatocytes.

    Fluorescence:

    Article Title: Targeting activated hepatic stellate cells through ferroptosis: phenethyl isothiocyanate attenuates CCl 4 -induced liver fibrosis by regulating iron metabolism, redox homeostasis, and autophagy.
    Article Snippet: Phenethyl isothiocyanate (PEITC), a dietary compound from cruciferous vegetables, is known for its chemopreventive activity, but its effects on liver fibrosis remain largely unexplored.. In this study, we show that PEITC administration attenuated carbon tetrachloride (CCl4)-induced hepatic injury and fibrosis in mice, as evidenced by reduced collagen deposition, hydroxyproline content and fibrotic marker expression, and improved liver function.. PEITC inhibited cell survival and fibrogenic phenotypes in transforming growth factor β (TGF-β)-stimulated LX-2 human hepatic stellate cells (HSCs) while exhibiting minimal cytotoxicity towards normal hepatocytes.

    Imaging:

    Article Title: Targeting activated hepatic stellate cells through ferroptosis: phenethyl isothiocyanate attenuates CCl 4 -induced liver fibrosis by regulating iron metabolism, redox homeostasis, and autophagy.
    Article Snippet: Phenethyl isothiocyanate (PEITC), a dietary compound from cruciferous vegetables, is known for its chemopreventive activity, but its effects on liver fibrosis remain largely unexplored.. In this study, we show that PEITC administration attenuated carbon tetrachloride (CCl4)-induced hepatic injury and fibrosis in mice, as evidenced by reduced collagen deposition, hydroxyproline content and fibrotic marker expression, and improved liver function.. PEITC inhibited cell survival and fibrogenic phenotypes in transforming growth factor β (TGF-β)-stimulated LX-2 human hepatic stellate cells (HSCs) while exhibiting minimal cytotoxicity towards normal hepatocytes.

    Article Title: Exercise attenuates stress-related signaling as sensed by higher phosphorylation of small heat shock proteins in skeletal muscle from older individuals
    Article Snippet: After a series of steps involving drying, re-activating, washing, and blocking, membranes were incubated in antibodies (for full details, see Latchman et al., ): firstly, anti-myosin heavy chain IIa (MHCIIa) (mouse, monoclonal IgG, A4.74; Developmental Studies Hybridoma Bank (DSHB), Iowa city, IA, USA), followed by stripping and then anti-MHCI (mouse monoclonal IgM, A4.840; DSHB). .. Chemiluminescent substrate (ECL, Clarity; Bio-Rad Laboratories, Sydney, Australia) enabled imaging of dots using a Chemidoc MP and Image Lab software (Bio-Rad Laboratories). .. Chemiluminescent substrate (ECL, Clarity; Bio-Rad Laboratories, Sydney, Australia) enabled imaging of dots using a Chemidoc MP and Image Lab software (Bio-Rad Laboratories).



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    Gene expression analysis of OCLN and markers related to its trafficking in WT and CB1 −/− (KO) testes. RTqPCR analysis of Ocln mRNA. Transcript amounts were reported as normalized fold expression (nfe) relatively to Rps18 gene (A). Western blot analysis of OCLN. Protein amounts were quantified by densitometry analysis, normalized against ERK1/2 signals and expressed in OD values (B). Immunofluorescence analysis of OCLN; white arrowheads represent OCLN localization (green) in testis. Nuclei were labelled with DAPI (blue). Scale bar: 5 µm (C). Quantitative analysis of OCLN green fluorescence on supra‐basal epithelium Regions of Interest (ROI); values are expressed as Sum of Intensity/area (SUM (I)/pixel 2 ), measured using the <t>Nikon</t> <t>Imaging</t> <t>Analytical</t> <t>Software</t> (NIS‐Elements Analysis D 4.40.00, 64 bit) (D). RTqPCR analysis of endocytic (Eea1), recycling (Rab13) and degradation (Rab9 and Itch) markers of OCLN. Transcript amounts were reported as normalized fold expression (nfe) relatively to Rps18 gene (E). All data were reported as mean value ± S.E.M. (* p < 0.05; ** p < 0.01).
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    Gene expression analysis of OCLN and markers related to its trafficking in WT and CB1 −/− (KO) testes. RTqPCR analysis of Ocln mRNA. Transcript amounts were reported as normalized fold expression (nfe) relatively to Rps18 gene (A). Western blot analysis of OCLN. Protein amounts were quantified by densitometry analysis, normalized against ERK1/2 signals and expressed in OD values (B). Immunofluorescence analysis of OCLN; white arrowheads represent OCLN localization (green) in testis. Nuclei were labelled with DAPI (blue). Scale bar: 5 µm (C). Quantitative analysis of OCLN green fluorescence on supra‐basal epithelium Regions of Interest (ROI); values are expressed as Sum of Intensity/area (SUM (I)/pixel 2 ), measured using the <t>Nikon</t> <t>Imaging</t> <t>Analytical</t> <t>Software</t> (NIS‐Elements Analysis D 4.40.00, 64 bit) (D). RTqPCR analysis of endocytic (Eea1), recycling (Rab13) and degradation (Rab9 and Itch) markers of OCLN. Transcript amounts were reported as normalized fold expression (nfe) relatively to Rps18 gene (E). All data were reported as mean value ± S.E.M. (* p < 0.05; ** p < 0.01).
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    Image Search Results


    Gene expression analysis of OCLN and markers related to its trafficking in WT and CB1 −/− (KO) testes. RTqPCR analysis of Ocln mRNA. Transcript amounts were reported as normalized fold expression (nfe) relatively to Rps18 gene (A). Western blot analysis of OCLN. Protein amounts were quantified by densitometry analysis, normalized against ERK1/2 signals and expressed in OD values (B). Immunofluorescence analysis of OCLN; white arrowheads represent OCLN localization (green) in testis. Nuclei were labelled with DAPI (blue). Scale bar: 5 µm (C). Quantitative analysis of OCLN green fluorescence on supra‐basal epithelium Regions of Interest (ROI); values are expressed as Sum of Intensity/area (SUM (I)/pixel 2 ), measured using the Nikon Imaging Analytical Software (NIS‐Elements Analysis D 4.40.00, 64 bit) (D). RTqPCR analysis of endocytic (Eea1), recycling (Rab13) and degradation (Rab9 and Itch) markers of OCLN. Transcript amounts were reported as normalized fold expression (nfe) relatively to Rps18 gene (E). All data were reported as mean value ± S.E.M. (* p < 0.05; ** p < 0.01).

    Journal: Journal of Cellular Physiology

    Article Title: Cannabinoid Receptors Modulate Physiological Remodelling of the Blood–Testis Barrier

    doi: 10.1002/jcp.70109

    Figure Lengend Snippet: Gene expression analysis of OCLN and markers related to its trafficking in WT and CB1 −/− (KO) testes. RTqPCR analysis of Ocln mRNA. Transcript amounts were reported as normalized fold expression (nfe) relatively to Rps18 gene (A). Western blot analysis of OCLN. Protein amounts were quantified by densitometry analysis, normalized against ERK1/2 signals and expressed in OD values (B). Immunofluorescence analysis of OCLN; white arrowheads represent OCLN localization (green) in testis. Nuclei were labelled with DAPI (blue). Scale bar: 5 µm (C). Quantitative analysis of OCLN green fluorescence on supra‐basal epithelium Regions of Interest (ROI); values are expressed as Sum of Intensity/area (SUM (I)/pixel 2 ), measured using the Nikon Imaging Analytical Software (NIS‐Elements Analysis D 4.40.00, 64 bit) (D). RTqPCR analysis of endocytic (Eea1), recycling (Rab13) and degradation (Rab9 and Itch) markers of OCLN. Transcript amounts were reported as normalized fold expression (nfe) relatively to Rps18 gene (E). All data were reported as mean value ± S.E.M. (* p < 0.05; ** p < 0.01).

    Article Snippet: The green fluorescence signal diffused in the luminal compartment was quantified using the Nikon Imaging Analytical Software (NIS‐Elements Analysis D 4.40.00, 64 bit) (see Figure ).

    Techniques: Gene Expression, Expressing, Western Blot, Immunofluorescence, Fluorescence, Imaging, Software

    Responsiveness of OCLN expression to CB1 and CB2 receptors. Western blot analysis of OCLN in testis of HT mice in vitro treated with vehicle (CTRL), ACEA (A) or JWH‐133 (B) at different concentrations: 0.1–1–10 µM. Protein amounts were quantified by densitometry analysis, normalized against ERK1/2 signals and expressed in OD as fold change. Immunofluorescence analysis of OCLN in testis of CTRL and ACEA (10 µM) or JWH‐133 (1 µM); white arrowheads represent OCLN localization (green) in testis. Nuclei were labelled with DAPI (blue). Scale bar: 5 µm (C). Quantitative analysis of OCLN green fluorescence on supra‐basal epithelium Regions of Interest (ROI); values are expressed as Sum of Intensity/area (SUM (I)/pixel 2 ), measured using the Nikon Imaging Analytical Software (NIS‐Elements Analysis D 4.40.00, 64 bit) (D). RTqPCR analysis of Ocln mRNA in testis of CTRL and ACEA 10 µM (E) or JWH‐133 1 µM (F). Transcript amounts were reported as normalized fold expression (nfe) relatively to Rps18 gene. All the data were reported as mean value ± S.E.M. (* p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001; a vs. b: p < 0.05).

    Journal: Journal of Cellular Physiology

    Article Title: Cannabinoid Receptors Modulate Physiological Remodelling of the Blood–Testis Barrier

    doi: 10.1002/jcp.70109

    Figure Lengend Snippet: Responsiveness of OCLN expression to CB1 and CB2 receptors. Western blot analysis of OCLN in testis of HT mice in vitro treated with vehicle (CTRL), ACEA (A) or JWH‐133 (B) at different concentrations: 0.1–1–10 µM. Protein amounts were quantified by densitometry analysis, normalized against ERK1/2 signals and expressed in OD as fold change. Immunofluorescence analysis of OCLN in testis of CTRL and ACEA (10 µM) or JWH‐133 (1 µM); white arrowheads represent OCLN localization (green) in testis. Nuclei were labelled with DAPI (blue). Scale bar: 5 µm (C). Quantitative analysis of OCLN green fluorescence on supra‐basal epithelium Regions of Interest (ROI); values are expressed as Sum of Intensity/area (SUM (I)/pixel 2 ), measured using the Nikon Imaging Analytical Software (NIS‐Elements Analysis D 4.40.00, 64 bit) (D). RTqPCR analysis of Ocln mRNA in testis of CTRL and ACEA 10 µM (E) or JWH‐133 1 µM (F). Transcript amounts were reported as normalized fold expression (nfe) relatively to Rps18 gene. All the data were reported as mean value ± S.E.M. (* p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001; a vs. b: p < 0.05).

    Article Snippet: The green fluorescence signal diffused in the luminal compartment was quantified using the Nikon Imaging Analytical Software (NIS‐Elements Analysis D 4.40.00, 64 bit) (see Figure ).

    Techniques: Expressing, Western Blot, In Vitro, Immunofluorescence, Fluorescence, Imaging, Software

    CB1 and CB2 action on BTB permeability and functionality. FITC‐dextran diffusion assay in testis of WT and HT mice in vitro treated with vehicle (CTRL), ACEA 10 µM and JWH‐133 1 µM (A) Scale bar: 300 μm. The green fluorescence signal diffused in the luminal compartment was quantified using the Nikon Imaging Analytical Software (NIS‐Elements Analysis D 4.40.00, 64 bit) as SUM (I)/pixel 2 (see also Figure for technical details) (B) a versus b: p < 0.05. RTqPCR analysis of endocytic (Eea1), recycling (Rab13) and degradation (Rab9 and Itch) markers of OCLN in the testis of CTRL and ACEA 10 µM (C) or JWH‐133 1 µM (D). Transcript amounts were reported as normalized fold expression (nfe) relatively to Rps18 gene. All data were reported as mean value ± S.E.M. (* p < 0.05; ** p < 0.01).

    Journal: Journal of Cellular Physiology

    Article Title: Cannabinoid Receptors Modulate Physiological Remodelling of the Blood–Testis Barrier

    doi: 10.1002/jcp.70109

    Figure Lengend Snippet: CB1 and CB2 action on BTB permeability and functionality. FITC‐dextran diffusion assay in testis of WT and HT mice in vitro treated with vehicle (CTRL), ACEA 10 µM and JWH‐133 1 µM (A) Scale bar: 300 μm. The green fluorescence signal diffused in the luminal compartment was quantified using the Nikon Imaging Analytical Software (NIS‐Elements Analysis D 4.40.00, 64 bit) as SUM (I)/pixel 2 (see also Figure for technical details) (B) a versus b: p < 0.05. RTqPCR analysis of endocytic (Eea1), recycling (Rab13) and degradation (Rab9 and Itch) markers of OCLN in the testis of CTRL and ACEA 10 µM (C) or JWH‐133 1 µM (D). Transcript amounts were reported as normalized fold expression (nfe) relatively to Rps18 gene. All data were reported as mean value ± S.E.M. (* p < 0.05; ** p < 0.01).

    Article Snippet: The green fluorescence signal diffused in the luminal compartment was quantified using the Nikon Imaging Analytical Software (NIS‐Elements Analysis D 4.40.00, 64 bit) (see Figure ).

    Techniques: Permeability, Diffusion-based Assay, In Vitro, Fluorescence, Imaging, Software, Expressing