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cell proliferation analysis gc 2spd ts cells  (Dojindo Labs)


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    Structured Review

    Dojindo Labs cell proliferation analysis gc 2spd ts cells
    Effect of down-regulating MRE11 and RAD50 on cell proliferation and apoptosis of <t>GC-2spd(ts)</t> cells. a Down-regulation of MRE11 and RAD50 by transfecting GC-2spd(ts) cells with siRNA was analyzed through western blotting using GAPDH as the loading control. b Proliferation of GC-2spd(ts) cells after transfection with MRE11 or RAD50 siRNA was measured by the CCK8 assay. Apoptosis of GC-2spd(ts) cells after siRNA transfection for 72 h was analyzed by c flow cytometry and d TUNEL assays. The scale bars represent 40 μm. Each assay was performed in triplicate. Data are presented as means ± SD. *P < 0.05.
    Cell Proliferation Analysis Gc 2spd Ts Cells, supplied by Dojindo Labs, used in various techniques. Bioz Stars score: 99/100, based on 60488 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/analysis+ts/pmc07056783-97-0-41
    Average 99 stars, based on 60488 article reviews
    cell proliferation analysis gc 2spd ts cells - by Bioz Stars, 2026-10
    99/100 stars

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    1) Product Images from "Decreased expression of MRE11 and RAD50 in testes from humans with spermatogenic failure"

    Article Title: Decreased expression of MRE11 and RAD50 in testes from humans with spermatogenic failure

    Journal: Journal of Assisted Reproduction and Genetics

    doi: 10.1007/s10815-019-01686-5

    Effect of down-regulating MRE11 and RAD50 on cell proliferation and apoptosis of GC-2spd(ts) cells. a Down-regulation of MRE11 and RAD50 by transfecting GC-2spd(ts) cells with siRNA was analyzed through western blotting using GAPDH as the loading control. b Proliferation of GC-2spd(ts) cells after transfection with MRE11 or RAD50 siRNA was measured by the CCK8 assay. Apoptosis of GC-2spd(ts) cells after siRNA transfection for 72 h was analyzed by c flow cytometry and d TUNEL assays. The scale bars represent 40 μm. Each assay was performed in triplicate. Data are presented as means ± SD. *P < 0.05.
    Figure Legend Snippet: Effect of down-regulating MRE11 and RAD50 on cell proliferation and apoptosis of GC-2spd(ts) cells. a Down-regulation of MRE11 and RAD50 by transfecting GC-2spd(ts) cells with siRNA was analyzed through western blotting using GAPDH as the loading control. b Proliferation of GC-2spd(ts) cells after transfection with MRE11 or RAD50 siRNA was measured by the CCK8 assay. Apoptosis of GC-2spd(ts) cells after siRNA transfection for 72 h was analyzed by c flow cytometry and d TUNEL assays. The scale bars represent 40 μm. Each assay was performed in triplicate. Data are presented as means ± SD. *P < 0.05.

    Techniques Used: Western Blot, Control, Transfection, CCK-8 Assay, Flow Cytometry, TUNEL Assay

    Related Articles

    Cell Culture:

    Article Title: Suppression of pseudogene MT2P1 transcription induced by E2F7 inhibits hepatocellular carcinoma cell proliferation and facilitates apoptosis via preserving its parental gene.
    Article Snippet: .. Cell proliferation and cell cycle detection The intervened HCC cells (1×106) were cultured in 96-well microtiter plates triplicated and incubated at an atmosphere of 5% CO2 and 37°C for 5 d. Microplate computer software (BioRad Laboratories, Inc., Hercules, CA, USA) was used for the OD values following the Cell Counting Kit-8 (CCK-8) assay kit protocol (Dojindo, Tokyo, Japan). ..

    Incubation:

    Article Title: Suppression of pseudogene MT2P1 transcription induced by E2F7 inhibits hepatocellular carcinoma cell proliferation and facilitates apoptosis via preserving its parental gene.
    Article Snippet: .. Cell proliferation and cell cycle detection The intervened HCC cells (1×106) were cultured in 96-well microtiter plates triplicated and incubated at an atmosphere of 5% CO2 and 37°C for 5 d. Microplate computer software (BioRad Laboratories, Inc., Hercules, CA, USA) was used for the OD values following the Cell Counting Kit-8 (CCK-8) assay kit protocol (Dojindo, Tokyo, Japan). ..

    Software:

    Article Title: Suppression of pseudogene MT2P1 transcription induced by E2F7 inhibits hepatocellular carcinoma cell proliferation and facilitates apoptosis via preserving its parental gene.
    Article Snippet: .. Cell proliferation and cell cycle detection The intervened HCC cells (1×106) were cultured in 96-well microtiter plates triplicated and incubated at an atmosphere of 5% CO2 and 37°C for 5 d. Microplate computer software (BioRad Laboratories, Inc., Hercules, CA, USA) was used for the OD values following the Cell Counting Kit-8 (CCK-8) assay kit protocol (Dojindo, Tokyo, Japan). ..

    CCK-8 Assay:

    Article Title: Suppression of pseudogene MT2P1 transcription induced by E2F7 inhibits hepatocellular carcinoma cell proliferation and facilitates apoptosis via preserving its parental gene.
    Article Snippet: .. Cell proliferation and cell cycle detection The intervened HCC cells (1×106) were cultured in 96-well microtiter plates triplicated and incubated at an atmosphere of 5% CO2 and 37°C for 5 d. Microplate computer software (BioRad Laboratories, Inc., Hercules, CA, USA) was used for the OD values following the Cell Counting Kit-8 (CCK-8) assay kit protocol (Dojindo, Tokyo, Japan). ..

    Article Title: Adverse predictive value of ASPM on lung adenocarcinoma overall survival depended on chemotherapy status.
    Article Snippet: .. Cell proliferation assay Cells were seeded into a 96-well plate at a density of 5 × 103 cells/well and treated with Cell Counting Kit-8 (CCK-8; CK04–100, dojindo, Kumamoto prefecture, Kyushu, Japan) for 2 h. the absorbance was measured with an ELx800 absorbance Reader (Biotek instruments, inc., Winooski, Vt, uSa) at 450 nm. ..

    Article Title: NNMT/1-MNA protects against hepatic ischemia-reperfusion injury through the AKT/FOXO1/ANGPT2/JNK axis.
    Article Snippet: .. Cell viability and apoptosis assay Cell viability was assessed by a Cell Counting Kit-8 assay (CCK-8, Dojindo Molecular Technologies, CK04–13). .. A lactate dehydrogenase (LDH) assay kit from Promega (Madison, WI, USA) was utilized to quantify the LDH concentration in the culture medium for evaluating cell death.

    Article Title: Tumor suppressing function of SLC16A7 in bladder cancer and its pan-cancer analysis.
    Article Snippet: .. Cell viability was evaluated every 24 h (at 24, 48, 72, and 96 h) using a Cell Counting Kit-8 (CCK8, Dojindo, Japan). ..

    Article Title: Polyoxyethylene and polypropylene emulsification enhances retinol palmitate efficacy in corneal wound healing.
    Article Snippet: The wound area was calculated using ImageJ software, and the wound closure rates were calculated as described above. .. Cell viability assays were performed using Cell Counting Kit-8 (CCK-8; Dojindo Laboratories, Tokyo, Japan) according to the manufacturer’s protocol. .. HCE-T cells were seeded into 96-well plates (Sumitomo Bakelite, Tokyo, Japan) at 2 × 104 cells/well, or 3D corneal epithelial cells were treated with solutions containing the ingredients indicated in the figure legends for 24, 48, and 72 h in a serum-free medium (DMEM/F-12 supplemented with 100 units/mL penicillin and 100 mg/mL streptomycin).

    Article Title: GSDME Deficiency Suppresses ER Stress-Induced Autophagic Cell Death via mTOR/S6K1 Activation in Hepatobiliary Carcinomas.
    Article Snippet: .. Cell Viability Assay Cell proliferation was quantified using the Cell Counting Kit-8 (CCK-8®; Dojindo Laboratories, Cat# CK04, Kumamoto, Japan). ..

    Article Title: SRSF1-mediated alternative splicing regulates bladder cancer progression and cisplatin sensitivity through HIF1A/BNIP3/mitophagy axis.
    Article Snippet: .. Cell viability was assessed every 24 h (24, 48, 72, and 96 h) using the Cell Counting Kit-8 (CCK-8, Dojindo, Japan). .. Absorbance at 450 nm was measured using a microplate reader (Tecan, Switzerland).

    Proliferation Assay:

    Article Title: Adverse predictive value of ASPM on lung adenocarcinoma overall survival depended on chemotherapy status.
    Article Snippet: .. Cell proliferation assay Cells were seeded into a 96-well plate at a density of 5 × 103 cells/well and treated with Cell Counting Kit-8 (CCK-8; CK04–100, dojindo, Kumamoto prefecture, Kyushu, Japan) for 2 h. the absorbance was measured with an ELx800 absorbance Reader (Biotek instruments, inc., Winooski, Vt, uSa) at 450 nm. ..

    Microplate Reader Absorbance Measurement:

    Article Title: Adverse predictive value of ASPM on lung adenocarcinoma overall survival depended on chemotherapy status.
    Article Snippet: .. Cell proliferation assay Cells were seeded into a 96-well plate at a density of 5 × 103 cells/well and treated with Cell Counting Kit-8 (CCK-8; CK04–100, dojindo, Kumamoto prefecture, Kyushu, Japan) for 2 h. the absorbance was measured with an ELx800 absorbance Reader (Biotek instruments, inc., Winooski, Vt, uSa) at 450 nm. ..

    Cell Counting:

    Article Title: Anti-transferrin receptor 1 antibody reduces angiotensin II-induced vascular remodeling.
    Article Snippet: The cell culture medium was replaced with serum-free medium for 24 h. Then, HASMCs were pretreated with or without antihuman TfR1 antibody (PPMX-T003; 1000 ng/mL, Perseus Proteomics Inc). .. After 24 h, HASMCs were treated with 0.1 μM AngII in the presence or absence of PPMX-T003 for 24 h. Cell proliferation was examined by a cell counting using cell counting kit 8 according to the manufacturer’s instructions (Dojindo, Kumamoto, Japan). .. In addition, intracellular ferrous iron (Fe2+) was assessed by FerroOrange staining according to the manufacturer’s instructions (Dojindo).

    Apoptosis Assay:

    Article Title: NNMT/1-MNA protects against hepatic ischemia-reperfusion injury through the AKT/FOXO1/ANGPT2/JNK axis.
    Article Snippet: .. Cell viability and apoptosis assay Cell viability was assessed by a Cell Counting Kit-8 assay (CCK-8, Dojindo Molecular Technologies, CK04–13). .. A lactate dehydrogenase (LDH) assay kit from Promega (Madison, WI, USA) was utilized to quantify the LDH concentration in the culture medium for evaluating cell death.

    Viability Assay:

    Article Title: GSDME Deficiency Suppresses ER Stress-Induced Autophagic Cell Death via mTOR/S6K1 Activation in Hepatobiliary Carcinomas.
    Article Snippet: .. Cell Viability Assay Cell proliferation was quantified using the Cell Counting Kit-8 (CCK-8®; Dojindo Laboratories, Cat# CK04, Kumamoto, Japan). ..



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    Dojindo Labs cell proliferation analysis gc 2spd ts cells
    Effect of down-regulating MRE11 and RAD50 on cell proliferation and apoptosis of <t>GC-2spd(ts)</t> cells. a Down-regulation of MRE11 and RAD50 by transfecting GC-2spd(ts) cells with siRNA was analyzed through western blotting using GAPDH as the loading control. b Proliferation of GC-2spd(ts) cells after transfection with MRE11 or RAD50 siRNA was measured by the CCK8 assay. Apoptosis of GC-2spd(ts) cells after siRNA transfection for 72 h was analyzed by c flow cytometry and d TUNEL assays. The scale bars represent 40 μm. Each assay was performed in triplicate. Data are presented as means ± SD. *P < 0.05.
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    Image Search Results


    Effect of down-regulating MRE11 and RAD50 on cell proliferation and apoptosis of GC-2spd(ts) cells. a Down-regulation of MRE11 and RAD50 by transfecting GC-2spd(ts) cells with siRNA was analyzed through western blotting using GAPDH as the loading control. b Proliferation of GC-2spd(ts) cells after transfection with MRE11 or RAD50 siRNA was measured by the CCK8 assay. Apoptosis of GC-2spd(ts) cells after siRNA transfection for 72 h was analyzed by c flow cytometry and d TUNEL assays. The scale bars represent 40 μm. Each assay was performed in triplicate. Data are presented as means ± SD. *P < 0.05.

    Journal: Journal of Assisted Reproduction and Genetics

    Article Title: Decreased expression of MRE11 and RAD50 in testes from humans with spermatogenic failure

    doi: 10.1007/s10815-019-01686-5

    Figure Lengend Snippet: Effect of down-regulating MRE11 and RAD50 on cell proliferation and apoptosis of GC-2spd(ts) cells. a Down-regulation of MRE11 and RAD50 by transfecting GC-2spd(ts) cells with siRNA was analyzed through western blotting using GAPDH as the loading control. b Proliferation of GC-2spd(ts) cells after transfection with MRE11 or RAD50 siRNA was measured by the CCK8 assay. Apoptosis of GC-2spd(ts) cells after siRNA transfection for 72 h was analyzed by c flow cytometry and d TUNEL assays. The scale bars represent 40 μm. Each assay was performed in triplicate. Data are presented as means ± SD. *P < 0.05.

    Article Snippet: Cell proliferation analysis GC-2spd(ts) cells were seeded and cultured in triplicate for each group at 3 × 10 3 cells/well in 96-well plates and reached 60–70% confluence after 24 h. Cell proliferation was determined using the Cell Counting Kit-8 (CCK-8) assay (Dojindo Molecular Technologies, Shanghai, China) according to the manufacturer’s protocol at 24, 48, and 72 h after siRNA transfection.

    Techniques: Western Blot, Control, Transfection, CCK-8 Assay, Flow Cytometry, TUNEL Assay