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Effect of down-regulating MRE11 and RAD50 on cell proliferation and apoptosis of GC-2spd(ts) cells. a Down-regulation of MRE11 and RAD50 by transfecting GC-2spd(ts) cells with siRNA was analyzed through western blotting using GAPDH as the loading control. b Proliferation of GC-2spd(ts) cells after transfection with MRE11 or RAD50 siRNA was measured by the CCK8 assay. Apoptosis of GC-2spd(ts) cells after siRNA transfection for 72 h was analyzed by c flow cytometry and d TUNEL assays. The scale bars represent 40 μm. Each assay was performed in triplicate. Data are presented as means ± SD. *P < 0.05.

Journal: Journal of Assisted Reproduction and Genetics

Article Title: Decreased expression of MRE11 and RAD50 in testes from humans with spermatogenic failure

doi: 10.1007/s10815-019-01686-5

Figure Lengend Snippet: Effect of down-regulating MRE11 and RAD50 on cell proliferation and apoptosis of GC-2spd(ts) cells. a Down-regulation of MRE11 and RAD50 by transfecting GC-2spd(ts) cells with siRNA was analyzed through western blotting using GAPDH as the loading control. b Proliferation of GC-2spd(ts) cells after transfection with MRE11 or RAD50 siRNA was measured by the CCK8 assay. Apoptosis of GC-2spd(ts) cells after siRNA transfection for 72 h was analyzed by c flow cytometry and d TUNEL assays. The scale bars represent 40 μm. Each assay was performed in triplicate. Data are presented as means ± SD. *P < 0.05.

Article Snippet: Cell proliferation analysis GC-2spd(ts) cells were seeded and cultured in triplicate for each group at 3 × 10 3 cells/well in 96-well plates and reached 60–70% confluence after 24 h. Cell proliferation was determined using the Cell Counting Kit-8 (CCK-8) assay (Dojindo Molecular Technologies, Shanghai, China) according to the manufacturer’s protocol at 24, 48, and 72 h after siRNA transfection.

Techniques: Western Blot, Control, Transfection, CCK-8 Assay, Flow Cytometry, TUNEL Assay