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2470 arrayer high-throughput microarray printing equipment  (Quanterix)


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    Quanterix 2470 arrayer high-throughput microarray printing equipment
    2470 Arrayer High Throughput Microarray Printing Equipment, supplied by Quanterix, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/2470+microarray/2470+arrayer/ppr0652443-65-51-56
    Average 90 stars, based on 1 article reviews
    2470 arrayer high-throughput microarray printing equipment - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    other:

    Article Title: Crosstalk with lung fibroblasts shapes the growth and therapeutic response of mesothelioma cells.
    Article Snippet: Samples were denatured by heating to 95 °C for 5 min prior to printing as serial dilutions (1.50 mg/ml, 0.75mg/ml, 0.375mg/ml and 0.1875mg/ml) in arrays consisting of 36 × 12 spots at a 500 μm spot-to-spot distance on the Aushon 2470 Arrayer Platform (Quanterix, MA, USA) using 8 × 185 μM pins, with 2 deposition rounds per feature.

    Article Title: Virtual Screening and Reality Verification:Elemene Injectable Emulsion acts on the key targets and pathways of Colorectal Adenoma Cancerization
    Article Snippet: 2.3.2 Differential protein data acquisition and pathway enrichment analysis by RPPA technology The quality of the sample is controlled by protein quality(the protein content is more signi cant than 1.5μg/μl).Serial gradient dilution (1,1/2,1/4,1/8,1/16) was carried out with uent 480/780 (Tecan,Swit), and more than 350 protein microarray chips were prepared with 2470 arrayer high-throughput microarray printing equipment (Quanterix,MA,USA).

    Article Title: Targeting of CYP2E1 by miRNAs in alcohol-induced intestine injury
    Article Snippet: GST proteins were then arrayed, in duplicate, using an Aushon 2470 Microarrayer (Quanterix) onto nitrocellulose-coated glass slides (Grace Bio-Labs).

    Article Title: Dynamic phospho-proteogenomic analysis of gastric cancer cells suggests host immunity provides survival benefit
    Article Snippet: The dots were printed on nitrocellulose-embedded glass slides (Grace BioLabs, Bend, OR, USA) with an Aushon 2470 Microarrayer (Quanterix, Billerica, MA, USA).

    Article Title: Targeting of CYP2E1 by miRNAs in alcohol-induced intestine injury.
    Article Snippet: GST proteins were then arrayed, in duplicate, using an Aushon 2470 Microarrayer (Quanterix) onto nitrocellulose-coated glass slides (Grace Bio-Labs).

    Control:

    Article Title: SOD1 is a synthetic-lethal target in PPM1D -mutant leukemia cells
    Article Snippet: .. The Quanterix 2470 Arrayer (Quanterix, Billerica, MA, USA) with a 40 pin (185 μm) configuration was used to spot samples and control lysates onto nitrocellulose-coated slides (Grace Bio-Labs, Bend, OR, USA) using an array format of 960 lysates/slide (2880 spots/slide). .. The slides were processed as described and probed with a set of 264 antibodies against total proteins and phosphoproteins using an automated slide stainer Autolink 48 (Dako, Santa Clara, CA, USA).

    Article Title: ESR1 and p53 interactome alteration defines mechanisms of tamoxifen response in luminal breast cancer
    Article Snippet: .. The Quanterix 2470 Arrayer (Quanterix, Billerica, MA) with a 40 pin (185 μm) configuration was used to spot samples and control lysates onto nitrocellulose-coated slides (Grace Bio-labs, Bend, OR) using an array format of 960 lysates/slide (2880 spots/slide). .. The slides were processed and probed with a set of 210 antibodies against total proteins and phosphoproteins using an automated slide stainer Autolink 48 (Dako, Santa Clara, CA, USA).

    Membrane:

    Article Title: Mitochondrial reprogramming by activating OXPHOS via glutamine metabolism in African American patients with bladder cancer
    Article Snippet: .. Subsequently, the protein extracts were printed on to nitrocellulose membrane-coated slides (Grace Bio-Labs) by Quanterix 2470 Arrayer (Quanterix Billerica). ..



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    Image Search Results


    Protein microarray data indicate that phosphorylation of S298 in maCx35 may act as a functional switch. ( a ) Membrane topology of connexins. N- and C-terminal tails (NT and CT, respectively) and the cytoplasmic loop (CL) reside inside the cytoplasm. The two extracellular loops (EL) are also indicated. ( b ) Sequence of the C-terminal end of maCx35/mmCx36, as indicated by the box in ( a ). Sequence identity (*) is high for mmCx36 and maCx35 at the C-terminal end. The blue box indicates a PKA/CaMKII consensus site at position S315 and S298 for mmCx36 and maCx35, respectively. The last four amino acids represent a PDZ binding domain, present in both proteins. ( c ) Microarray layout (A-D); the respective baits (all fused to GST) are found below. An array probed with Alexa Fluor 555 anti-GST antibody served as positive control. Peptides fused to biotin containing the C-terminal tail of maCx35 (35 WT), phosphorylated maCx35 (35 S298 phos), and a truncated version (35 S298 ter) were used as probes and showed differential results, marked by rounded squares. 14–3–3 proteins (red) and NHERF2 aa265-288 (blue D7) showed an increased binding to the 35 S298 phospho probe compared to 35 WT. In contrast, PDZK1, PDZ10 of MUPP1, and nNOS (blue) decreased their binding to the 35 S298 phospho probe. The truncated 35 S298 ter probe showed no binding interactions.

    Journal: Scientific Reports

    Article Title: Phosphorylation of Connexin36 near the C-terminus switches binding affinities for PDZ-domain and 14–3–3 proteins in vitro

    doi: 10.1038/s41598-020-75375-0

    Figure Lengend Snippet: Protein microarray data indicate that phosphorylation of S298 in maCx35 may act as a functional switch. ( a ) Membrane topology of connexins. N- and C-terminal tails (NT and CT, respectively) and the cytoplasmic loop (CL) reside inside the cytoplasm. The two extracellular loops (EL) are also indicated. ( b ) Sequence of the C-terminal end of maCx35/mmCx36, as indicated by the box in ( a ). Sequence identity (*) is high for mmCx36 and maCx35 at the C-terminal end. The blue box indicates a PKA/CaMKII consensus site at position S315 and S298 for mmCx36 and maCx35, respectively. The last four amino acids represent a PDZ binding domain, present in both proteins. ( c ) Microarray layout (A-D); the respective baits (all fused to GST) are found below. An array probed with Alexa Fluor 555 anti-GST antibody served as positive control. Peptides fused to biotin containing the C-terminal tail of maCx35 (35 WT), phosphorylated maCx35 (35 S298 phos), and a truncated version (35 S298 ter) were used as probes and showed differential results, marked by rounded squares. 14–3–3 proteins (red) and NHERF2 aa265-288 (blue D7) showed an increased binding to the 35 S298 phospho probe compared to 35 WT. In contrast, PDZK1, PDZ10 of MUPP1, and nNOS (blue) decreased their binding to the 35 S298 phospho probe. The truncated 35 S298 ter probe showed no binding interactions.

    Article Snippet: Purified GST fusion proteins (listed in Fig. c and Supplementary Fig. ) were spotted onto nitrocellulose-coated glass slides with an Aushon 2470 microarray robot; each protein was spotted in duplicate.

    Techniques: Microarray, Functional Assay, Sequencing, Binding Assay, Positive Control

    Materials

    Journal: Journal of visualized experiments : JoVE

    Article Title: Analysis of Histone Antibody Specificity with Peptide Microarrays

    doi: 10.3791/55912

    Figure Lengend Snippet: Materials

    Article Snippet: contact microarray printer , Aushon , 2470 , Aushon 2470 Microarray Printer.

    Techniques: Microscopy, Microarray, Blocking Assay, Plasmid Preparation, Hybridization, Peptide Microarray, Modification, High Throughput Screening Assay

    A screen shot of the ArrayNinja design module is shown in the dotted line. The control panel (top) shows all of the parameters that can be altered on the microarray printer. As these parameters are adjusted, the cartoon image of the slide layout (bottom left) updates in real time. After the layout is set, the user can mouse over individual spots to enter unique feature identifiers. ArrayNinja constructs from this user input a map of the position of each feature in the source plate(s) (bottom right) needed to fabricate a specified microarray slide layout.

    Journal: Journal of visualized experiments : JoVE

    Article Title: Analysis of Histone Antibody Specificity with Peptide Microarrays

    doi: 10.3791/55912

    Figure Lengend Snippet: A screen shot of the ArrayNinja design module is shown in the dotted line. The control panel (top) shows all of the parameters that can be altered on the microarray printer. As these parameters are adjusted, the cartoon image of the slide layout (bottom left) updates in real time. After the layout is set, the user can mouse over individual spots to enter unique feature identifiers. ArrayNinja constructs from this user input a map of the position of each feature in the source plate(s) (bottom right) needed to fabricate a specified microarray slide layout.

    Article Snippet: contact microarray printer , Aushon , 2470 , Aushon 2470 Microarray Printer.

    Techniques: Microarray, Construct

    (A) Histone peptide microarray fabrication on streptavidin-coated microscope slides using a contact microarray printer. (B) Microarrays fabricated with 3 subarrays of a 48 × 48 grid of peptide features. Separation of (C) 3 subarrays with a hydrophobic wax pen, (D) 2 subarrays with a silicon adhesive, and (E) 48 subarrays with a wax imprint. All microarrays shown are fabricated using 25 × 75 mm microscope slides.

    Journal: Journal of visualized experiments : JoVE

    Article Title: Analysis of Histone Antibody Specificity with Peptide Microarrays

    doi: 10.3791/55912

    Figure Lengend Snippet: (A) Histone peptide microarray fabrication on streptavidin-coated microscope slides using a contact microarray printer. (B) Microarrays fabricated with 3 subarrays of a 48 × 48 grid of peptide features. Separation of (C) 3 subarrays with a hydrophobic wax pen, (D) 2 subarrays with a silicon adhesive, and (E) 48 subarrays with a wax imprint. All microarrays shown are fabricated using 25 × 75 mm microscope slides.

    Article Snippet: contact microarray printer , Aushon , 2470 , Aushon 2470 Microarray Printer.

    Techniques: Peptide Microarray, Microscopy, Microarray