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sureprint g3 human cgh 4×180k microarray slides  (Agilent technologies)


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    Agilent technologies sureprint g3 human cgh 4×180k microarray slides
    Sureprint G3 Human Cgh 4×180k Microarray Slides, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/180k+microarray+slides/pmc05830403-441-22-29
    Average 90 stars, based on 1 article reviews
    sureprint g3 human cgh 4×180k microarray slides - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Hybridization:

    Article Title: Engineering microdeletions and microduplications by targeting segmental duplications with CRISPR
    Article Snippet: .. Array-based comparative genomic hybridization (aCGH) was performed on the Agilent 4×180K SurePrint G3 Human CGH Microarray (Design #022060) according to the protocol provided by the manufacturer. .. The assay assessed for imbalances (i.e., gains/losses) in the genomic DNA sample being tested by comparing the test DNA to a Promega male control DNA sample obtained from a pool of karyotypically normal individual(s).

    Article Title: Single cell polarity in liquid phase facilitates tumour metastasis
    Article Snippet: .. Labelling of CTC DNA and corresponding reference DNA from WGA libraries of single leukocytes of a healthy female donor and hybridisation on SurePrint G3 Human CGH 4×180K microarray slides (Agilent Technologies, design code 022060) were performed according to the manufacturer’s protocol (Agilent Oligonucleotide Array-Based CGH for Genomic DNA Analysis, version 7.1, December 2011) . .. Slides were scanned using an Agilent Microarray Scanner Type C, and images were processed with Agilent Genomic Feature Extraction Software (version 10.7) and imported and analysed with the Agilent Genomic Workbench Software (version 6.5 lite).

    Microarray:

    Article Title: Engineering microdeletions and microduplications by targeting segmental duplications with CRISPR
    Article Snippet: .. Array-based comparative genomic hybridization (aCGH) was performed on the Agilent 4×180K SurePrint G3 Human CGH Microarray (Design #022060) according to the protocol provided by the manufacturer. .. The assay assessed for imbalances (i.e., gains/losses) in the genomic DNA sample being tested by comparing the test DNA to a Promega male control DNA sample obtained from a pool of karyotypically normal individual(s).

    Article Title: Single cell polarity in liquid phase facilitates tumour metastasis
    Article Snippet: .. Labelling of CTC DNA and corresponding reference DNA from WGA libraries of single leukocytes of a healthy female donor and hybridisation on SurePrint G3 Human CGH 4×180K microarray slides (Agilent Technologies, design code 022060) were performed according to the manufacturer’s protocol (Agilent Oligonucleotide Array-Based CGH for Genomic DNA Analysis, version 7.1, December 2011) . .. Slides were scanned using an Agilent Microarray Scanner Type C, and images were processed with Agilent Genomic Feature Extraction Software (version 10.7) and imported and analysed with the Agilent Genomic Workbench Software (version 6.5 lite).

    Article Title: Integrative analysis of the microRNA-mRNA response to radiochemotherapy in primary head and neck squamous cell carcinoma cells
    Article Snippet: .. For array CGH analysis of the primary cell cultures the SurePrint G3 human CGH Microarray Kit 4x180k (Agilent Technologies, Santa Clara, CA, AMADID: 022060) was used. .. Tumor DNA (250 ng) and sex-mismatched normal reference DNA (250 ng) (Promega, Madison, WI) were used for hybridization.

    Whole Genome Amplification:

    Article Title: Single cell polarity in liquid phase facilitates tumour metastasis
    Article Snippet: .. Labelling of CTC DNA and corresponding reference DNA from WGA libraries of single leukocytes of a healthy female donor and hybridisation on SurePrint G3 Human CGH 4×180K microarray slides (Agilent Technologies, design code 022060) were performed according to the manufacturer’s protocol (Agilent Oligonucleotide Array-Based CGH for Genomic DNA Analysis, version 7.1, December 2011) . .. Slides were scanned using an Agilent Microarray Scanner Type C, and images were processed with Agilent Genomic Feature Extraction Software (version 10.7) and imported and analysed with the Agilent Genomic Workbench Software (version 6.5 lite).



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    Image Search Results


    (a-b) LncRNA microarray expression data from BMDMs incubated in distinct polarizing conditions. A total of 1,251 differentially-expressed lncRNAs were identified between the M(LPS+IFN-γ) and M(IL-4) macrophages. (a) Scatter plots show the variation in lncRNA expression between the M(LPS+IFN-γ) and M(IL-4) macrophages. The values of the X and Y axes in the scatter plot are the averaged normalized values in each group (log2-scaled). (b) Heat maps of lncRNAs expression profiles between the two groups. “Red” indicates high relative expression and “green” indicates low relative expression. One ANOVA test was used for statistical analysis. LncRNA with expression fold change > 2 and with FDR-adiusted P value < 0.05 was considered statistically significant. (c) Confirmation of the differential expression of lncRNAs by RT-qPCR. Four differentially expressed lncRNAs were validated by RT-qPCR. The y-axis represents the log2-transformed median fold-change in expression. (d) LncRNA Dnmt3aos locates on the Dnmt3a opposite strand on mouse chromosome 12. (e) RT-qPCR detection of Dnmt3aos level in cellular fractions from BMDMs. U6 and GAPDH were the nuclear and cytoplasmic controls, respectively. Data were expressed as the means ± SD of three independent experiments. (f) Dnmt3aos expression in BMDMs detected by RNA-FISH. Scale bar, 20 mm.

    Journal: bioRxiv

    Article Title: LncRNA Dnmt3aos regulates Dnmt3a expression leading to aberrant DNA methylation in macrophage polarization

    doi: 10.1101/514307

    Figure Lengend Snippet: (a-b) LncRNA microarray expression data from BMDMs incubated in distinct polarizing conditions. A total of 1,251 differentially-expressed lncRNAs were identified between the M(LPS+IFN-γ) and M(IL-4) macrophages. (a) Scatter plots show the variation in lncRNA expression between the M(LPS+IFN-γ) and M(IL-4) macrophages. The values of the X and Y axes in the scatter plot are the averaged normalized values in each group (log2-scaled). (b) Heat maps of lncRNAs expression profiles between the two groups. “Red” indicates high relative expression and “green” indicates low relative expression. One ANOVA test was used for statistical analysis. LncRNA with expression fold change > 2 and with FDR-adiusted P value < 0.05 was considered statistically significant. (c) Confirmation of the differential expression of lncRNAs by RT-qPCR. Four differentially expressed lncRNAs were validated by RT-qPCR. The y-axis represents the log2-transformed median fold-change in expression. (d) LncRNA Dnmt3aos locates on the Dnmt3a opposite strand on mouse chromosome 12. (e) RT-qPCR detection of Dnmt3aos level in cellular fractions from BMDMs. U6 and GAPDH were the nuclear and cytoplasmic controls, respectively. Data were expressed as the means ± SD of three independent experiments. (f) Dnmt3aos expression in BMDMs detected by RNA-FISH. Scale bar, 20 mm.

    Article Snippet: Each SBC mouse (4*180K) LncRNA microarray slide (Agilent Technologies Inc.) was hybridized with 1.65 μg Cy3-labeled cRNA using a gene expression hybridization kit (Agilent Technologies, Inc.).

    Techniques: Microarray, Expressing, Incubation, Quantitative RT-PCR, Transformation Assay