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Image Search Results
Journal: PLOS Biology
Article Title: Structure of G protein-coupled receptor GPR1 bound to full-length chemerin adipokine reveals a chemokine-like reverse binding mode
doi: 10.1371/journal.pbio.3002838
Figure Lengend Snippet: (A) cAMP inhibition mediated by GPR1 and CMKLR1 in response to different concentrations of chemerin and C9. (B) G protein dissociation assay based on NanoBiT technology in transfected cells that express GPR1 and CMKLR1, respectively, and stimulated with chemerin and C9 at different concentrations. Control: HBSS without ligand addition. (C) IP-one accumulation in cells expressing GPR1 and CMKLR1, respectively, in response to chemerin and C9 at different concentrations. (D) β-arrestin recruitment upon chemerin and C9 stimulation based on NanoBiT technology, in transfected cells expressing GPR1 and CMKLR1, respectively. Data shown are means ± SEM from 3 independent experiments. The underlying data can be found in .
Article Snippet: The cells were resuspended in the stimulation buffer (
Techniques: Inhibition, Transfection, Control, Expressing