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BUHLMANN Laboratories stimulation buffer
Stimulation Buffer, supplied by BUHLMANN Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/stimulating+buffer/stimulation+buffer/pmc06597347-127-55-63
Average 90 stars, based on 1 article reviews
stimulation buffer - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

Concentration Assay:

Article Title: Clinical and immunological differences between asymptomatic HDM-sensitized and HDM-allergic rhinitis patients
Article Snippet: .. 22 Briefly, whole blood samples were stimulated with D pteronyssinus allergen extract containing a final concentration of 0.333, 3.33, 33.3 or 333 ng/mL of allergen (Buhlmann Laboratories, Schönenbuch, Switzerland); 0.55 μg/mL of anti-FcεRI mAb (Buhlmann Laboratories); or 2 μmol/L /mL l -formyl- l -methionyl- l -leucyl- l -phenylalanine (fMLP; Sigma-Aldrich, St. Louis, MO, USA) or stimulation buffer alone (containing 3 ng/mL of IL-3; Buhlmann Laboratories). ..

Sedimentation:

Article Title: Correlation between cysteinyl leukotriene release from leukocytes and clinical response to a leukotriene inhibitor.
Article Snippet: Study objectives: Antileukotriene drugs are widely used in patients with bronchial asthma, but not all patients show significant clinical improvements, and no factors have been identified that are correlated with the clinical response to these drugs.. This study was designed to examine the factors correlated with a response to a leukotriene receptor antagonist, pranlukast, in patients with asthma.. Design: WBC counts, IgE, and ex vivo leukotriene release from leukocytes were measured, and 31 patients with asthma were treated with pranlukast, a leukotriene receptor antagonist, for 4 weeks.

Binding Assay:

Article Title: IgE and IgG4 Repertoire in Asymptomatic HDM-Sensitized and HDM-Induced Allergic Rhinitis Patients
Article Snippet: .. N-formyl-methionyl-leucyl-phenylalanine (fMLP; BUHLMANN Laboratories AG, CH-4124 Schönenbuch, Switzerland) and highly specific monoclonal antibody binding to the high-affinity IgE receptor (anti-FcεRI mAb; BUHLMANN Laboratories AG, CH-4124 Schönenbuch, Switzerland) were used as positive controls, while 50 μL stimulation buffer (containing 3 ng/mL IL-3; BUHLMANN Laboratories AG, CH-4124 Schönenbuch, Switzerland) was used as negative control. ..

Negative Control:

Article Title: IgE and IgG4 Repertoire in Asymptomatic HDM-Sensitized and HDM-Induced Allergic Rhinitis Patients
Article Snippet: .. N-formyl-methionyl-leucyl-phenylalanine (fMLP; BUHLMANN Laboratories AG, CH-4124 Schönenbuch, Switzerland) and highly specific monoclonal antibody binding to the high-affinity IgE receptor (anti-FcεRI mAb; BUHLMANN Laboratories AG, CH-4124 Schönenbuch, Switzerland) were used as positive controls, while 50 μL stimulation buffer (containing 3 ng/mL IL-3; BUHLMANN Laboratories AG, CH-4124 Schönenbuch, Switzerland) was used as negative control. ..



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(A) cAMP inhibition mediated by GPR1 and CMKLR1 in response to different concentrations of chemerin and C9. (B) G protein dissociation assay based on NanoBiT technology in transfected cells that express GPR1 and CMKLR1, respectively, and stimulated with chemerin and C9 at different concentrations. Control: HBSS without ligand addition. (C) IP-one accumulation in cells expressing GPR1 and CMKLR1, respectively, in response to chemerin and C9 at different concentrations. (D) β-arrestin recruitment upon chemerin and C9 <t>stimulation</t> based on NanoBiT technology, in transfected cells expressing GPR1 and CMKLR1, respectively. Data shown are means ± SEM from 3 independent experiments. The underlying data can be found in .
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(A) cAMP inhibition mediated by GPR1 and CMKLR1 in response to different concentrations of chemerin and C9. (B) G protein dissociation assay based on NanoBiT technology in transfected cells that express GPR1 and CMKLR1, respectively, and stimulated with chemerin and C9 at different concentrations. Control: HBSS without ligand addition. (C) IP-one accumulation in cells expressing GPR1 and CMKLR1, respectively, in response to chemerin and C9 at different concentrations. (D) β-arrestin recruitment upon chemerin and C9 <t>stimulation</t> based on NanoBiT technology, in transfected cells expressing GPR1 and CMKLR1, respectively. Data shown are means ± SEM from 3 independent experiments. The underlying data can be found in .
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Image Search Results


(A) cAMP inhibition mediated by GPR1 and CMKLR1 in response to different concentrations of chemerin and C9. (B) G protein dissociation assay based on NanoBiT technology in transfected cells that express GPR1 and CMKLR1, respectively, and stimulated with chemerin and C9 at different concentrations. Control: HBSS without ligand addition. (C) IP-one accumulation in cells expressing GPR1 and CMKLR1, respectively, in response to chemerin and C9 at different concentrations. (D) β-arrestin recruitment upon chemerin and C9 stimulation based on NanoBiT technology, in transfected cells expressing GPR1 and CMKLR1, respectively. Data shown are means ± SEM from 3 independent experiments. The underlying data can be found in .

Journal: PLOS Biology

Article Title: Structure of G protein-coupled receptor GPR1 bound to full-length chemerin adipokine reveals a chemokine-like reverse binding mode

doi: 10.1371/journal.pbio.3002838

Figure Lengend Snippet: (A) cAMP inhibition mediated by GPR1 and CMKLR1 in response to different concentrations of chemerin and C9. (B) G protein dissociation assay based on NanoBiT technology in transfected cells that express GPR1 and CMKLR1, respectively, and stimulated with chemerin and C9 at different concentrations. Control: HBSS without ligand addition. (C) IP-one accumulation in cells expressing GPR1 and CMKLR1, respectively, in response to chemerin and C9 at different concentrations. (D) β-arrestin recruitment upon chemerin and C9 stimulation based on NanoBiT technology, in transfected cells expressing GPR1 and CMKLR1, respectively. Data shown are means ± SEM from 3 independent experiments. The underlying data can be found in .

Article Snippet: The cells were resuspended in the stimulation buffer (Cisbio) and incubated with different concentrations of C9 peptide diluted in the stimulation buffer for 30 min at 37°C.

Techniques: Inhibition, Transfection, Control, Expressing