stimulation buffer (Cisbio Bioassays)
86
Structured Review
Cisbio Bioassays
stimulation buffer
Stimulation Buffer, supplied by Cisbio Bioassays, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/stimulating+buffer/stimulation+buf/pmc11515964-125-6-8
Average 86 stars, based on 1 article reviews
Stimulation Buffer, supplied by Cisbio Bioassays, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/stimulating+buffer/stimulation+buf/pmc11515964-125-6-8
Average 86 stars, based on 1 article reviews
stimulation buffer - by Bioz Stars,
2026-11
86/100 stars
Images
1) Product Images from "Structure of G protein-coupled receptor GPR1 bound to full-length chemerin adipokine reveals a chemokine-like reverse binding mode"
Article Title: Structure of G protein-coupled receptor GPR1 bound to full-length chemerin adipokine reveals a chemokine-like reverse binding mode
Journal: PLOS Biology
doi: 10.1371/journal.pbio.3002838
Figure Legend Snippet: (A) cAMP inhibition mediated by GPR1 and CMKLR1 in response to different concentrations of chemerin and C9. (B) G protein dissociation assay based on NanoBiT technology in transfected cells that express GPR1 and CMKLR1, respectively, and stimulated with chemerin and C9 at different concentrations. Control: HBSS without ligand addition. (C) IP-one accumulation in cells expressing GPR1 and CMKLR1, respectively, in response to chemerin and C9 at different concentrations. (D) β-arrestin recruitment upon chemerin and C9 stimulation based on NanoBiT technology, in transfected cells expressing GPR1 and CMKLR1, respectively. Data shown are means ± SEM from 3 independent experiments. The underlying data can be found in .
Techniques Used: Inhibition, Transfection, Control, Expressing
Related Articles
Concentration Assay:Article Title: Inhibiting a promiscuous GPCR: iterative discovery of bitter taste receptor ligands Article Snippet: .. Then, the library compounds and controls, dissolved in DMSO, were prediluted to twofold final concentration into 96 deep-well plates (nerbe plus, Winsen/Luhe, Germany) containing Article Title: Inhibiting a promiscuous GPCR: iterative discovery of bitter taste receptor ligands Article Snippet: .. Then, the library compounds and controls, dissolved in DMSO, were prediluted to 2-fold final concentration into 96 deep-well plates (nerbe plus, Winsen/Luhe, Germany) containing other:Article Title: Structure of an LGR dimer – an evolutionary predecessor of glycoprotein hormone receptors Article Snippet: Next, cells were harvested with centrifugation at 200 xg for 5 min, washed with phosphate-buffered saline (PBS), resuspended in stimulating buffer (Cisbio), seeded onto a 384-well plate and incubated with serially diluted ligand for 2 h at 37 °C in the presence of 8% CO 2 . Article Title: Bruton's Tyrosine Kinase Inhibitors with Distinct Binding Modes Reveal Differential Functional Impact on B-Cell Receptor Signaling Article Snippet: Inositol phosphate 1 homogeneous time-resolved fluorescence assay Compounds were added in a 384-well low-volume plate using D300e digital dispenser (TECAN), and 50,000 Ramos cells (wildtype and BTK knockout) were incubated with compounds in cell growth medium for 4 hours before 10 mg/mL goat F(ab’)2 antihuman IgM (Southern Biotech, catalog no. 2022–01) was added in stimulation buffer (IP-One kit, Cisbio, catalog no. 40451). Incubation:Article Title: Structure of G protein-coupled receptor GPR1 bound to full-length chemerin adipokine reveals a chemokine-like reverse binding mode. Article Snippet: WT GPR1 and its mutants, and WT human CMKLR1 for comparison, were transiently expressed in HEK293T cells for 24 h. IP1 accumulation was tested using the IP-One Gq HTRF kit (Cisbio). .. The cells were resuspended in the Article Title: Structure of G protein-coupled receptor GPR1 bound to full-length chemerin adipokine reveals a chemokine-like reverse binding mode Article Snippet: WT GPR1 and its mutants, and WT human CMKLR1 for comparison, were transiently expressed in HEK293T cells for 24 h. IP1 accumulation was tested using the IP-One Gq HTRF kit (Cisbio). .. The cells were resuspended in the Beta-Arrestin Assay:Article Title: Discovery of Novel and Selective GPR17 Antagonists as Pharmacological Tools for Developing New Therapeutic Strategies in Diabetes and Obesity Article Snippet: .. Cells were serum starved for 2 h and treated with varying concentrations of test compound and vehicle for 10 min in |