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stimulation buffer  (Becton Dickinson)

 
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    Structured Review

    Becton Dickinson stimulation buffer
    Stimulation Buffer, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/stimulating+buffer/stimulation+buffer/us08822407-264-18-26
    Average 90 stars, based on 1 article reviews
    stimulation buffer - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Incubation:

    Article Title: Preparation of conjugates comprising adenine derivatives and allergenic proteins and their use for specific immunotherapy of allengenic diseases
    Article Snippet: .. 100 μl whole blood from four allergic patients sensitized to Dermatophagoides pteronyssinus were incubated together with 20 μl stimulation buffer (negative control) provided by the manufacturer (Becton Dickinson) or 20 μl serial dilutions of DP or nDer p2 or their respective conjugates DP-conj or nDer p2-conj (0.5-0.05-0.005 μg/ml) or 20 μl fMLP or 20 μg anti-IgE monoclonal antibody as positive control for 15 min at 37° C. in water. ..

    Negative Control:

    Article Title: Preparation of conjugates comprising adenine derivatives and allergenic proteins and their use for specific immunotherapy of allengenic diseases
    Article Snippet: .. 100 μl whole blood from four allergic patients sensitized to Dermatophagoides pteronyssinus were incubated together with 20 μl stimulation buffer (negative control) provided by the manufacturer (Becton Dickinson) or 20 μl serial dilutions of DP or nDer p2 or their respective conjugates DP-conj or nDer p2-conj (0.5-0.05-0.005 μg/ml) or 20 μl fMLP or 20 μg anti-IgE monoclonal antibody as positive control for 15 min at 37° C. in water. ..

    Positive Control:

    Article Title: Preparation of conjugates comprising adenine derivatives and allergenic proteins and their use for specific immunotherapy of allengenic diseases
    Article Snippet: .. 100 μl whole blood from four allergic patients sensitized to Dermatophagoides pteronyssinus were incubated together with 20 μl stimulation buffer (negative control) provided by the manufacturer (Becton Dickinson) or 20 μl serial dilutions of DP or nDer p2 or their respective conjugates DP-conj or nDer p2-conj (0.5-0.05-0.005 μg/ml) or 20 μl fMLP or 20 μg anti-IgE monoclonal antibody as positive control for 15 min at 37° C. in water. ..



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    Image Search Results


    (A) cAMP inhibition mediated by GPR1 and CMKLR1 in response to different concentrations of chemerin and C9. (B) G protein dissociation assay based on NanoBiT technology in transfected cells that express GPR1 and CMKLR1, respectively, and stimulated with chemerin and C9 at different concentrations. Control: HBSS without ligand addition. (C) IP-one accumulation in cells expressing GPR1 and CMKLR1, respectively, in response to chemerin and C9 at different concentrations. (D) β-arrestin recruitment upon chemerin and C9 stimulation based on NanoBiT technology, in transfected cells expressing GPR1 and CMKLR1, respectively. Data shown are means ± SEM from 3 independent experiments. The underlying data can be found in .

    Journal: PLOS Biology

    Article Title: Structure of G protein-coupled receptor GPR1 bound to full-length chemerin adipokine reveals a chemokine-like reverse binding mode

    doi: 10.1371/journal.pbio.3002838

    Figure Lengend Snippet: (A) cAMP inhibition mediated by GPR1 and CMKLR1 in response to different concentrations of chemerin and C9. (B) G protein dissociation assay based on NanoBiT technology in transfected cells that express GPR1 and CMKLR1, respectively, and stimulated with chemerin and C9 at different concentrations. Control: HBSS without ligand addition. (C) IP-one accumulation in cells expressing GPR1 and CMKLR1, respectively, in response to chemerin and C9 at different concentrations. (D) β-arrestin recruitment upon chemerin and C9 stimulation based on NanoBiT technology, in transfected cells expressing GPR1 and CMKLR1, respectively. Data shown are means ± SEM from 3 independent experiments. The underlying data can be found in .

    Article Snippet: The cells were resuspended in the stimulation buffer (Cisbio) and incubated with different concentrations of C9 peptide diluted in the stimulation buffer for 30 min at 37°C.

    Techniques: Inhibition, Transfection, Control, Expressing