Journal: eLife
Article Title: A transient role of the ciliary gene Inpp5e in controlling direct versus indirect neurogenesis in cortical development
doi: 10.7554/eLife.58162
Figure Lengend Snippet:
Article Snippet: Immunofluorescence staining was performed on 12 to 14 μm cryostat sections as described previously ( ) with antibodies against Arl13b (mouse) (Neuromab 75–287; 1:1500), rat anti-BrdU (1:50, Abcam #ab6326), mouse anti-BrdU/IdU (B44) (1:50, BD Biosciences #347580), rabbit anti-Cleaved Caspase 3 (1:100, Cell Signaling Technology, #9664), rat anti-Ctip2 (1:1000, Abcam #ab18465), rabbit anti-GFAP (1:1000, Agilent/Dako #Z 0334), rat anti-L1, clone 324 (1:1000, Millipore #MAB5272), rabbit anti-Pax6 (1:400, Biolegend #901301), mouse anti-PCNA (1:500, Abcam #ab29), rabbit anti-Prox1 (1:1000, RELIA Tech #102-PA32). rabbit anti-pHH3 (1:100, Millipore #06–570), mouse anti-Satb2 (1:200, Abcam #ab51502), rabbit anti-Tbr1 (1:400, Abcam #ab31940), rabbit anti-Tbr2 (1:1000, Abcam #ab23345) and rabbit anti-γTUB (Sigma-Aldrich SAB4503045; 1:100).
Techniques: Plasmid Preparation, Software