β2m expressed hct15  (ATCC)

Journal: Molecular Medicine Reports

Article Title: Development of an innovative approach for early diagnosis of cervical cancer using TCR-like antibodies targeting HPV18 E6 and E7 peptides

doi: 10.3892/mmr.2025.13583

Figure Lengend Snippet: Sequence analysis of the β2m chain. β2m-T7 promoter and terminator sequencing results (300 bp on each side) were compared with the original β2m chain map. Sanger sequencing was conducted using T7 vector primers. β2m, β-2-microglobulin.

Article Snippet: Wells were rinsed three times with 1X PBS-T and 100 μl anti-β2m HRP (1:3,000; Cat. No. GTX40576; GeneTex, Inc.) was added to each well.

Techniques: Sequencing, Plasmid Preparation

Journal: Molecular Medicine Reports

Article Title: Development of an innovative approach for early diagnosis of cervical cancer using TCR-like antibodies targeting HPV18 E6 and E7 peptides

doi: 10.3892/mmr.2025.13583

Figure Lengend Snippet: Evaluation of P-MHC-A2402 complex components. (A) Agarose gel electrophoresis visualizing the PCR amplification of HLA-A24 and β2m chains. L, 1 KB plus DNA ladder; 1, HLA-A24 chain at ~1,144 bp; 2, β2m chain at ~495 bp; 3, negative control (ddH 2 O). (B) SDS-PAGE analysis of inclusion body fractions of heavy and light chains. L, pre-stained protein ladder (9–245 kDa); 1, HLA-A24 fraction band at ~35 kDa or β2m fraction band at ~12 kDa. HLA-ELISA analysis of the stability of the generated p-MHC complex. The OD 405 nm results indicate successful formation of the HPV18 (C) E6 and (D) E7 p-MHC-A24 complexes. ***P<0.001. HLA, human leukocyte antigen; β2m, β-2-microglobulin; OD, optical density.

Article Snippet: Wells were rinsed three times with 1X PBS-T and 100 μl anti-β2m HRP (1:3,000; Cat. No. GTX40576; GeneTex, Inc.) was added to each well.

Techniques: Agarose Gel Electrophoresis, Amplification, Negative Control, SDS Page, Staining, Enzyme-linked Immunosorbent Assay, Generated