Journal: Molecular Systems Biology
Article Title: Proteome-wide copy-number estimation from transcriptomics
doi: 10.1038/s44320-024-00064-3
Figure Lengend Snippet: ( A , B ) Knockdown of NUP37 increases CDK4 abundance in B2B1 breast epithelial cells (Wang et al, ). ( C , D ) Overexpression of inducible NUP37 (iNUP37) decreases CDK4 abundance in B2B1 breast epithelial cells. ( E , F ) Overexpression of iNUP37 decreases CDK4 abundance in MCF7 luminal breast cancer cells. ( G , H ) Residual DNM1L protein after knockdown correlates with CDK4 abundance in T47D ( G ) and ZR751 ( H ) luminal breast cancer cells. Actin was used as a housekeeping control not used for loading normalization (“Methods”). Quantities are scaled to shScramble control samples. Data information: For ( A , C , E ), representative immunoblots for NUP37 and CDK4 are shown with vinculin and tubulin ( A , C ) or p38 ( E ) as loading controls and FLAG ( C , E ) to confirm ectopic expression of iNUP37 or luciferase (luc) control. For ( B , D , F ), immunoblot results are summarized as the mean total NUP37 (endogenous ( B , D , F ) and induced ( D , F )) or CDK4 ± s.e.m. of n = 6 biological replicates. Differences were assessed by one-sided t test. For ( G , H ), representative immunoblots for DNM1L and CDK4 are shown with vinculin and p38 as loading controls and actin as a housekeeping control. Data are summaries from n = 6 biological replicates.
Article Snippet: ZR751 cells ( H. sapiens ) , ATCC , Cat # CRL-1500.
Techniques: Knockdown, Over Expression, Control, Western Blot, Expressing, Luciferase
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