znf410 (Proteintech)
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93
Structured Review
Proteintech
znf410
Znf410, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/znf410/ZNF410+Antibody/bio_rxiv__64898__2026__02__06__704516-299-39-40
Average 93 stars, based on 8 article reviews
Znf410, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/znf410/ZNF410+Antibody/bio_rxiv__64898__2026__02__06__704516-299-39-40
Average 93 stars, based on 8 article reviews
znf410 - by Bioz Stars,
2026-09
93/100 stars
Images
1) Product Images from "Regulation of BCL11A DNA binding and expression in human erythrocyte precursor HUDEP-2 cells"
Article Title: Regulation of BCL11A DNA binding and expression in human erythrocyte precursor HUDEP-2 cells
Journal: bioRxiv
doi: 10.64898/2026.02.06.704516
Figure Legend Snippet: Summary of pomalidomide- and lenalidomide-derivative compound screen for HbF inducers in HUDEP-2 cells and unbiased global proteomic follow-up. For screening, cells were treated at stages 1 and 2 and collected at stage 3 (see ). ( A ) Pomalidomide (Poma) treatment (1 µM, 6 days) in HUDEP-2 cells induces a 4-fold increase of %HbF + cells as quantified by flow cytometry (p < 0.0001, two-sided t-test). ( B ) A total of 19 compounds induced HbF levels to at least 50% of the internal pomalidomide positive control, as quantified by flow cytometry. Compounds discussed in text are highlighted in color. Compounds 1088 (4-carbon linker), 1089 (5-carbon linker) and 1091 (7-carbon linker) share chemical structure similarity with 1090 , which contains a 6-carbon linker (Supplementary Table S4). ( C ) Direct targets of pomalidomide in HUDEP-2 cells (indicated by asterisks) include known targets IKZF1 and FAM83F and novel targets FBXO22, RASSF5, CDIP1, and KCTD15 (Supplementary Table S6). ( D ) Compound 1075 led to specific degradation of IKZF1 and ZFP91. ( E ) Compound 1090 led to degradation of a subset of major pomalidomide targets, with increased specificity for ZFP91 and CYP2R1. ( F ) Compound 1126 led to degradation of a subset of pomalidomide targets with increased specificity for CYP2R1 and ZPF91 and decreased specificity for IKZF1. ( G ) Compound 1235 , a derivative of lenalidomide, led to degradation of the known lenalidomide targets (indicated by asterisks) IKZF1, CSNK1A1, and FAM83F, along with the novel targets CYP2R1, FIZ1, CDIP1, DAGLB, and ENTPD5. Two proteins (in red labels) were increased: GTF2H3 (FC = 1.67, adjusted p = 0.0022) and NFYA (FC = 2.27, adjusted p = 0.035). NFYA is necessary for hematopoietic stem cell proliferation . ( H ) Western blot of HUDEP-2 cells at stage 2 treated for 6 hours confirms degradation of IKZF1, shows no detectable degradation of BCL11A, and verifies the presence of ZNF410, which was not detected in the mass spectrometry (Supplementary Table S5). FC = fold change. AdjP = adjusted p-value.
Techniques Used: Flow Cytometry, Positive Control, Western Blot, Mass Spectrometry
Figure Legend Snippet: Models of BCL11A regulation across three complementary layers. ( A ) The ZF0-mediated oligomerization assembles different BCL11A splice variants (depicted as different lengths), all of which contain ZF0, enabling BCL11A to form high-order polymers. ( B ) The EED inhibitor FTX6058 reduces BCL11A dosage mainly through LIN28B, which modulates BCL11A translation. ( C ) Sequence alignment of known pomalidomide degrons (IKZF1 and ZFP91) and dWIZ-2 target (WIZ), and the corresponding ZF units of ZNF410 and BCL11A. Zinc-coordinating residues are colored blue, and residues critical for the selectivity of pomalidomide- or lenalidomide-derived degraders are shown in red. ( D ) The pomalidomide-derived compound 1075 induces degradation of ZFP91 and IKZF1, reshaping the regulatory network contributing to HbF induction.
Techniques Used: Sequencing, Derivative Assay
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Expressing:Article Title: ZNF410 represses fetal globin by singular control of CHD4. Article Snippet: .. Nuclear extracts from CD34+ HSPC-derived erythroblasts on day 11 of erythroid maturation culture were used to assess Chromatin Immunoprecipitation:Article Title: ZNF410 Uniquely Activates the NuRD Component CHD4 to Silence Fetal Hemoglobin Expression Article Snippet: .. Article Title: ZNF410 uniquely activates the NuRD component CHD4 to silence fetal hemoglobin expression Article Snippet: .. Western Blot:Article Title: ZNF410 uniquely activates the NuRD component CHD4 to silence fetal hemoglobin expression Article Snippet: .. Western blotting analysis was carried out according to standard protocol with the following antibodies: |
