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agonists yoda1  (MedChemExpress)


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    Structured Review

    MedChemExpress agonists yoda1
    Agonists Yoda1, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 97/100, based on 108 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/yoda1/GsMTx4/pmc13084373-57-3-13
    Average 97 stars, based on 108 article reviews
    agonists yoda1 - by Bioz Stars, 2026-10
    97/100 stars

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    Related Articles

    Concentration Assay:

    Article Title: Exploring CACNA1H as a Potential Candidate Biomarker for Calcific Aortic Valve Disease
    Article Snippet: .. A concentration of 5 μM Yoda1 (MedChemExpress, HY-18723, USA) was chosen to stimulate AVICs in vitro, thereby simulating mechanical stress to induce calcification of AVICs.11 The P65/S536D plasmid was obtained from Geneyuan Biotechnology. ..

    In Vitro:

    Article Title: Exploring CACNA1H as a Potential Candidate Biomarker for Calcific Aortic Valve Disease
    Article Snippet: .. A concentration of 5 μM Yoda1 (MedChemExpress, HY-18723, USA) was chosen to stimulate AVICs in vitro, thereby simulating mechanical stress to induce calcification of AVICs.11 The P65/S536D plasmid was obtained from Geneyuan Biotechnology. ..

    Plasmid Preparation:

    Article Title: Exploring CACNA1H as a Potential Candidate Biomarker for Calcific Aortic Valve Disease
    Article Snippet: .. A concentration of 5 μM Yoda1 (MedChemExpress, HY-18723, USA) was chosen to stimulate AVICs in vitro, thereby simulating mechanical stress to induce calcification of AVICs.11 The P65/S536D plasmid was obtained from Geneyuan Biotechnology. ..

    Cell Culture:

    Article Title: Stiff matrix promotes lung cancer cell migration through down-regulating the Piezo1 channel expression to facilitate Ca 2+ -dependent filopodia formation
    Article Snippet: .. In some cases, cells were cultured in medium containing 2 μM Yoda1 (#M9372, AbMole, USA) to activate the Piezo1 channel, 2.5 μM GsMTx4 (#HY-P1410, MedChemExpress, USA) to block the Piezo1 channel, 25 μM BAPTA-AM (#M4973, AbMole, USA) to chelate intracellular Ca 2+ or adding 2 mM CaCl 2 to observe extracellular Ca 2+ influx, 4 μM cyclosporine A (CsA) (#M1831, AbMole, USA) to inhibit CaN, 0.5 μM dasatinib (Das, #9052S, Cell signaling technology) to inhibit YAP nuclear translocation, as well as 10 μM PF-573228 (PF, #HY-10461, MCE) to inhibit focal adhesion kinase (FAK) activation, respectively. .. Cells were seeded into 6‐well plates with PA hydrogels-covered glass coverslips at a density of 2 × 10 4 cells/well, and cultured further for 48 h. Following fixation with 4 % paraformaldehyde and permeabilization with 0.1 % Triton-X, cells were incubated with rhodamine-labeled phalloidin (1:100 dilution, #C2207S, Beyotime, China) for 40 min. Hoechst 33342 (1:1000, #H3570, ThermoFisher, USA) was used to stain nuclei.

    Blocking Assay:

    Article Title: Stiff matrix promotes lung cancer cell migration through down-regulating the Piezo1 channel expression to facilitate Ca 2+ -dependent filopodia formation
    Article Snippet: .. In some cases, cells were cultured in medium containing 2 μM Yoda1 (#M9372, AbMole, USA) to activate the Piezo1 channel, 2.5 μM GsMTx4 (#HY-P1410, MedChemExpress, USA) to block the Piezo1 channel, 25 μM BAPTA-AM (#M4973, AbMole, USA) to chelate intracellular Ca 2+ or adding 2 mM CaCl 2 to observe extracellular Ca 2+ influx, 4 μM cyclosporine A (CsA) (#M1831, AbMole, USA) to inhibit CaN, 0.5 μM dasatinib (Das, #9052S, Cell signaling technology) to inhibit YAP nuclear translocation, as well as 10 μM PF-573228 (PF, #HY-10461, MCE) to inhibit focal adhesion kinase (FAK) activation, respectively. .. Cells were seeded into 6‐well plates with PA hydrogels-covered glass coverslips at a density of 2 × 10 4 cells/well, and cultured further for 48 h. Following fixation with 4 % paraformaldehyde and permeabilization with 0.1 % Triton-X, cells were incubated with rhodamine-labeled phalloidin (1:100 dilution, #C2207S, Beyotime, China) for 40 min. Hoechst 33342 (1:1000, #H3570, ThermoFisher, USA) was used to stain nuclei.

    Translocation Assay:

    Article Title: Stiff matrix promotes lung cancer cell migration through down-regulating the Piezo1 channel expression to facilitate Ca 2+ -dependent filopodia formation
    Article Snippet: .. In some cases, cells were cultured in medium containing 2 μM Yoda1 (#M9372, AbMole, USA) to activate the Piezo1 channel, 2.5 μM GsMTx4 (#HY-P1410, MedChemExpress, USA) to block the Piezo1 channel, 25 μM BAPTA-AM (#M4973, AbMole, USA) to chelate intracellular Ca 2+ or adding 2 mM CaCl 2 to observe extracellular Ca 2+ influx, 4 μM cyclosporine A (CsA) (#M1831, AbMole, USA) to inhibit CaN, 0.5 μM dasatinib (Das, #9052S, Cell signaling technology) to inhibit YAP nuclear translocation, as well as 10 μM PF-573228 (PF, #HY-10461, MCE) to inhibit focal adhesion kinase (FAK) activation, respectively. .. Cells were seeded into 6‐well plates with PA hydrogels-covered glass coverslips at a density of 2 × 10 4 cells/well, and cultured further for 48 h. Following fixation with 4 % paraformaldehyde and permeabilization with 0.1 % Triton-X, cells were incubated with rhodamine-labeled phalloidin (1:100 dilution, #C2207S, Beyotime, China) for 40 min. Hoechst 33342 (1:1000, #H3570, ThermoFisher, USA) was used to stain nuclei.

    Activation Assay:

    Article Title: Stiff matrix promotes lung cancer cell migration through down-regulating the Piezo1 channel expression to facilitate Ca 2+ -dependent filopodia formation
    Article Snippet: .. In some cases, cells were cultured in medium containing 2 μM Yoda1 (#M9372, AbMole, USA) to activate the Piezo1 channel, 2.5 μM GsMTx4 (#HY-P1410, MedChemExpress, USA) to block the Piezo1 channel, 25 μM BAPTA-AM (#M4973, AbMole, USA) to chelate intracellular Ca 2+ or adding 2 mM CaCl 2 to observe extracellular Ca 2+ influx, 4 μM cyclosporine A (CsA) (#M1831, AbMole, USA) to inhibit CaN, 0.5 μM dasatinib (Das, #9052S, Cell signaling technology) to inhibit YAP nuclear translocation, as well as 10 μM PF-573228 (PF, #HY-10461, MCE) to inhibit focal adhesion kinase (FAK) activation, respectively. .. Cells were seeded into 6‐well plates with PA hydrogels-covered glass coverslips at a density of 2 × 10 4 cells/well, and cultured further for 48 h. Following fixation with 4 % paraformaldehyde and permeabilization with 0.1 % Triton-X, cells were incubated with rhodamine-labeled phalloidin (1:100 dilution, #C2207S, Beyotime, China) for 40 min. Hoechst 33342 (1:1000, #H3570, ThermoFisher, USA) was used to stain nuclei.

    Article Title: Mechanically heterogeneous nanofibrous hydrogel drives Piezo1-mediated cell aggregation and collective migration.
    Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.

    other:

    Article Title: Age-mimicking hydrogel stiffness recapitulates the mechanical niche of the hippocampus to regulate neural stem cell senescence
    Article Snippet: After 24 h of culture, the medium was replaced with medium containing 2 μM Yoda1 (MCE, HY-18723) to treat the cells for 48 h. Subsequently, 15 μM EdU was added to the medium for 4 h to label proliferating cells.

    Functional Assay:

    Article Title: Mechanically heterogeneous nanofibrous hydrogel drives Piezo1-mediated cell aggregation and collective migration.
    Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.

    Inhibition:

    Article Title: Mechanically heterogeneous nanofibrous hydrogel drives Piezo1-mediated cell aggregation and collective migration.
    Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.

    Live Cell Imaging:

    Article Title: Mechanically heterogeneous nanofibrous hydrogel drives Piezo1-mediated cell aggregation and collective migration.
    Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.



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    Piezo1 participates in stiffness-induced SCC progression. (A) Volcano plots and heatmap showing differential expressed mechanoreceptors between soft and stiff matrices cultured A431 cSCC cells. (B) Co-expression of COL1A1/COL3A1 and PIEZO1 in spatial transcriptomic data of cSCC. (C) Schematic representation of performing gradient gene expression analysis. (D) Gradient gene expression analysis of COL1A1, COL3A1 and PIEZO1 from the tumor center to the periphery, detail see methods. (E) Spearman correlation between PIEZO1 and COL1A1/COL3A1 expression by spatial gradient. (F) The effects of changing matrix stiffness on Piezo1 mRNA levels (normalized to GAPDH, n = 4) in A431, XL50 and H520 cells. (G) The effects of changing matrix stiffness on Piezo1 protein levels (normalized to β-actin) in A431, XL50 and H520 cells. (H) Representative images of A431-shNC and A431-shPiezo1 spheroids cultured on soft, medium or stiff gels. Bar: 200 μm. (I) Quantification of the relative migration areas (calculated by migration area/spheroid area) of A431-shNC and A431-shPiezo1 spheroids cultured on soft, medium and stiff gels ( n = 3). (J) The effects of changing matrix stiffness on Ki67, PCNA, MMP2 and MMP9 protein levels (normalized to β-actin) in A431-shNC and A431-shPiezo1 cells. (K) Representative images of A431 spheroids cultured on soft or stiff gels treated with vehicle (DMSO) or 500 nM GsMTx-4 to inhibit Piezo1. Bar: 200 μm. (L) Quantification of the relative migration areas (calculated by migration area/spheroid area) of A431 spheroids cultured on soft or stiff gels treated with vehicle (DMSO) or 500 nM GsMTx-4 to inhibit Piezo1 ( n = 4). (M) The effects of changing matrix stiffness on Ki67, PCNA, MMP2 and MMP9 protein levels (normalized to β-actin) in A431 cells treated with vehicle (DMSO) or 5 μM <t>Yoda1</t> to activate Piezo1.
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    Piezo1 participates in stiffness-induced SCC progression. (A) Volcano plots and heatmap showing differential expressed mechanoreceptors between soft and stiff matrices cultured A431 cSCC cells. (B) Co-expression of COL1A1/COL3A1 and PIEZO1 in spatial transcriptomic data of cSCC. (C) Schematic representation of performing gradient gene expression analysis. (D) Gradient gene expression analysis of COL1A1, COL3A1 and PIEZO1 from the tumor center to the periphery, detail see methods. (E) Spearman correlation between PIEZO1 and COL1A1/COL3A1 expression by spatial gradient. (F) The effects of changing matrix stiffness on Piezo1 mRNA levels (normalized to GAPDH, n = 4) in A431, XL50 and H520 cells. (G) The effects of changing matrix stiffness on Piezo1 protein levels (normalized to β-actin) in A431, XL50 and H520 cells. (H) Representative images of A431-shNC and A431-shPiezo1 spheroids cultured on soft, medium or stiff gels. Bar: 200 μm. (I) Quantification of the relative migration areas (calculated by migration area/spheroid area) of A431-shNC and A431-shPiezo1 spheroids cultured on soft, medium and stiff gels ( n = 3). (J) The effects of changing matrix stiffness on Ki67, PCNA, MMP2 and MMP9 protein levels (normalized to β-actin) in A431-shNC and A431-shPiezo1 cells. (K) Representative images of A431 spheroids cultured on soft or stiff gels treated with vehicle (DMSO) or 500 nM GsMTx-4 to inhibit Piezo1. Bar: 200 μm. (L) Quantification of the relative migration areas (calculated by migration area/spheroid area) of A431 spheroids cultured on soft or stiff gels treated with vehicle (DMSO) or 500 nM GsMTx-4 to inhibit Piezo1 ( n = 4). (M) The effects of changing matrix stiffness on Ki67, PCNA, MMP2 and MMP9 protein levels (normalized to β-actin) in A431 cells treated with vehicle (DMSO) or 5 μM <t>Yoda1</t> to activate Piezo1.
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    Piezo1 participates in stiffness-induced SCC progression. (A) Volcano plots and heatmap showing differential expressed mechanoreceptors between soft and stiff matrices cultured A431 cSCC cells. (B) Co-expression of COL1A1/COL3A1 and PIEZO1 in spatial transcriptomic data of cSCC. (C) Schematic representation of performing gradient gene expression analysis. (D) Gradient gene expression analysis of COL1A1, COL3A1 and PIEZO1 from the tumor center to the periphery, detail see methods. (E) Spearman correlation between PIEZO1 and COL1A1/COL3A1 expression by spatial gradient. (F) The effects of changing matrix stiffness on Piezo1 mRNA levels (normalized to GAPDH, n = 4) in A431, XL50 and H520 cells. (G) The effects of changing matrix stiffness on Piezo1 protein levels (normalized to β-actin) in A431, XL50 and H520 cells. (H) Representative images of A431-shNC and A431-shPiezo1 spheroids cultured on soft, medium or stiff gels. Bar: 200 μm. (I) Quantification of the relative migration areas (calculated by migration area/spheroid area) of A431-shNC and A431-shPiezo1 spheroids cultured on soft, medium and stiff gels ( n = 3). (J) The effects of changing matrix stiffness on Ki67, PCNA, MMP2 and MMP9 protein levels (normalized to β-actin) in A431-shNC and A431-shPiezo1 cells. (K) Representative images of A431 spheroids cultured on soft or stiff gels treated with vehicle (DMSO) or 500 nM GsMTx-4 to inhibit Piezo1. Bar: 200 μm. (L) Quantification of the relative migration areas (calculated by migration area/spheroid area) of A431 spheroids cultured on soft or stiff gels treated with vehicle (DMSO) or 500 nM GsMTx-4 to inhibit Piezo1 ( n = 4). (M) The effects of changing matrix stiffness on Ki67, PCNA, MMP2 and MMP9 protein levels (normalized to β-actin) in A431 cells treated with vehicle (DMSO) or 5 μM <t>Yoda1</t> to activate Piezo1.
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    Image Search Results


    Piezo1 participates in stiffness-induced SCC progression. (A) Volcano plots and heatmap showing differential expressed mechanoreceptors between soft and stiff matrices cultured A431 cSCC cells. (B) Co-expression of COL1A1/COL3A1 and PIEZO1 in spatial transcriptomic data of cSCC. (C) Schematic representation of performing gradient gene expression analysis. (D) Gradient gene expression analysis of COL1A1, COL3A1 and PIEZO1 from the tumor center to the periphery, detail see methods. (E) Spearman correlation between PIEZO1 and COL1A1/COL3A1 expression by spatial gradient. (F) The effects of changing matrix stiffness on Piezo1 mRNA levels (normalized to GAPDH, n = 4) in A431, XL50 and H520 cells. (G) The effects of changing matrix stiffness on Piezo1 protein levels (normalized to β-actin) in A431, XL50 and H520 cells. (H) Representative images of A431-shNC and A431-shPiezo1 spheroids cultured on soft, medium or stiff gels. Bar: 200 μm. (I) Quantification of the relative migration areas (calculated by migration area/spheroid area) of A431-shNC and A431-shPiezo1 spheroids cultured on soft, medium and stiff gels ( n = 3). (J) The effects of changing matrix stiffness on Ki67, PCNA, MMP2 and MMP9 protein levels (normalized to β-actin) in A431-shNC and A431-shPiezo1 cells. (K) Representative images of A431 spheroids cultured on soft or stiff gels treated with vehicle (DMSO) or 500 nM GsMTx-4 to inhibit Piezo1. Bar: 200 μm. (L) Quantification of the relative migration areas (calculated by migration area/spheroid area) of A431 spheroids cultured on soft or stiff gels treated with vehicle (DMSO) or 500 nM GsMTx-4 to inhibit Piezo1 ( n = 4). (M) The effects of changing matrix stiffness on Ki67, PCNA, MMP2 and MMP9 protein levels (normalized to β-actin) in A431 cells treated with vehicle (DMSO) or 5 μM Yoda1 to activate Piezo1.

    Journal: Journal of Advanced Research

    Article Title: Matrix stiffness drives squamous cell carcinoma progression via a Piezo1-mediated mechanotransduction feedback loop

    doi: 10.1016/j.jare.2025.10.041

    Figure Lengend Snippet: Piezo1 participates in stiffness-induced SCC progression. (A) Volcano plots and heatmap showing differential expressed mechanoreceptors between soft and stiff matrices cultured A431 cSCC cells. (B) Co-expression of COL1A1/COL3A1 and PIEZO1 in spatial transcriptomic data of cSCC. (C) Schematic representation of performing gradient gene expression analysis. (D) Gradient gene expression analysis of COL1A1, COL3A1 and PIEZO1 from the tumor center to the periphery, detail see methods. (E) Spearman correlation between PIEZO1 and COL1A1/COL3A1 expression by spatial gradient. (F) The effects of changing matrix stiffness on Piezo1 mRNA levels (normalized to GAPDH, n = 4) in A431, XL50 and H520 cells. (G) The effects of changing matrix stiffness on Piezo1 protein levels (normalized to β-actin) in A431, XL50 and H520 cells. (H) Representative images of A431-shNC and A431-shPiezo1 spheroids cultured on soft, medium or stiff gels. Bar: 200 μm. (I) Quantification of the relative migration areas (calculated by migration area/spheroid area) of A431-shNC and A431-shPiezo1 spheroids cultured on soft, medium and stiff gels ( n = 3). (J) The effects of changing matrix stiffness on Ki67, PCNA, MMP2 and MMP9 protein levels (normalized to β-actin) in A431-shNC and A431-shPiezo1 cells. (K) Representative images of A431 spheroids cultured on soft or stiff gels treated with vehicle (DMSO) or 500 nM GsMTx-4 to inhibit Piezo1. Bar: 200 μm. (L) Quantification of the relative migration areas (calculated by migration area/spheroid area) of A431 spheroids cultured on soft or stiff gels treated with vehicle (DMSO) or 500 nM GsMTx-4 to inhibit Piezo1 ( n = 4). (M) The effects of changing matrix stiffness on Ki67, PCNA, MMP2 and MMP9 protein levels (normalized to β-actin) in A431 cells treated with vehicle (DMSO) or 5 μM Yoda1 to activate Piezo1.

    Article Snippet: GsMTx-4 (500 nM, Abcam) was used for inhibiting Piezo1, Yoda1 (5 μM, MedChemExpress) was applied for activating Piezo1, verteporfin (5 μM, Selleck) was applied for inhibiting YAP and PY-60 (10 μM, Selleck) was used for activating YAP.

    Techniques: Cell Culture, Expressing, Gene Expression, Migration

    YAP functions as the downstream molecule of Piezo1 in the process of stiffness-induced SCC progression. (A) The effects of changing matrix stiffness on YAP and TAZ mRNA levels (normalized to GAPDH) in A431 cells treated with vehicle (DMSO) or 5 μM Yoda1 to activate Piezo1 ( n = 3). (B) The effects of changing matrix stiffness on YAP and p-YAP protein levels (normalized to β-actin) in A431 cells treated with vehicle (DMSO) or 5 μM Yoda1 to activate Piezo1 ( n = 3). (C) The effects of changing matrix stiffness on KI67, PCNA, MMP2 and MMP9 mRNA levels (normalized to GAPDH) in A431 cells treated with vehicle (DMSO) or 10 μM verteporfin to inhibit YAP ( n = 3). (D) The effects of changing matrix stiffness on Ki67, PCNA, MMP2 and MMP9 protein levels (normalized to β-actin) in A431 cells treated with vehicle (DMSO) or 10 μM verteporfin to inhibit YAP. (E) The mRNA levels of KI67, PCNA, MMP2 and MMP9 (normalized to GAPDH) in scramble shRNA-transfected (shNC) and Piezo1 knockdown (shPiezo1) A431 cells cultured in stiff matrix treated with vehicle (DMSO) or 10 μM PY-60 to activate YAP ( n = 3). (F) The protein levels of Ki67, PCNA, MMP2 and MMP9 (normalized to β-actin) in scramble shRNA-transfected (shNC) and Piezo1 knockdown (shPiezo1) A431 cells cultured in stiff matrix treated with vehicle (DMSO) or 10 μM PY-60 to activate YAP. (G) The effects of changing matrix stiffness on MST1, MST2, LATS1 and LATS2 mRNA levels (normalized to GAPDH) in A431 cells treated with vehicle (DMSO) or 5 μM Yoda1 to activate Piezo1 ( n = 3). (H) The effects of changing matrix stiffness on LATS1 and p-LATS1 protein levels (normalized to β-actin) in A431 cells treated with vehicle (DMSO) or 5 μM Yoda1 to activate Piezo1.

    Journal: Journal of Advanced Research

    Article Title: Matrix stiffness drives squamous cell carcinoma progression via a Piezo1-mediated mechanotransduction feedback loop

    doi: 10.1016/j.jare.2025.10.041

    Figure Lengend Snippet: YAP functions as the downstream molecule of Piezo1 in the process of stiffness-induced SCC progression. (A) The effects of changing matrix stiffness on YAP and TAZ mRNA levels (normalized to GAPDH) in A431 cells treated with vehicle (DMSO) or 5 μM Yoda1 to activate Piezo1 ( n = 3). (B) The effects of changing matrix stiffness on YAP and p-YAP protein levels (normalized to β-actin) in A431 cells treated with vehicle (DMSO) or 5 μM Yoda1 to activate Piezo1 ( n = 3). (C) The effects of changing matrix stiffness on KI67, PCNA, MMP2 and MMP9 mRNA levels (normalized to GAPDH) in A431 cells treated with vehicle (DMSO) or 10 μM verteporfin to inhibit YAP ( n = 3). (D) The effects of changing matrix stiffness on Ki67, PCNA, MMP2 and MMP9 protein levels (normalized to β-actin) in A431 cells treated with vehicle (DMSO) or 10 μM verteporfin to inhibit YAP. (E) The mRNA levels of KI67, PCNA, MMP2 and MMP9 (normalized to GAPDH) in scramble shRNA-transfected (shNC) and Piezo1 knockdown (shPiezo1) A431 cells cultured in stiff matrix treated with vehicle (DMSO) or 10 μM PY-60 to activate YAP ( n = 3). (F) The protein levels of Ki67, PCNA, MMP2 and MMP9 (normalized to β-actin) in scramble shRNA-transfected (shNC) and Piezo1 knockdown (shPiezo1) A431 cells cultured in stiff matrix treated with vehicle (DMSO) or 10 μM PY-60 to activate YAP. (G) The effects of changing matrix stiffness on MST1, MST2, LATS1 and LATS2 mRNA levels (normalized to GAPDH) in A431 cells treated with vehicle (DMSO) or 5 μM Yoda1 to activate Piezo1 ( n = 3). (H) The effects of changing matrix stiffness on LATS1 and p-LATS1 protein levels (normalized to β-actin) in A431 cells treated with vehicle (DMSO) or 5 μM Yoda1 to activate Piezo1.

    Article Snippet: GsMTx-4 (500 nM, Abcam) was used for inhibiting Piezo1, Yoda1 (5 μM, MedChemExpress) was applied for activating Piezo1, verteporfin (5 μM, Selleck) was applied for inhibiting YAP and PY-60 (10 μM, Selleck) was used for activating YAP.

    Techniques: shRNA, Transfection, Knockdown, Cell Culture

    RCC2 is essential to Piezo1-induced YAP activation in stiff matrix. (A) Uniform Manifold Approximation and Projection (UMAP) plots showing the major cell types in shNC ( n = 2) and shPiezo1 ( n = 2) cSCC tumor tissues. (B) Percentage and Ro/e of major cell types in shNC and shPiezo1 tumor tissue. (C) Four-quadrant plot displaying DEGs between shNC and shPiezo1 malignant cell and correlations between genes and Piezo1 to identified Piezo1-YAP regulators. (D) The effects of changing matrix stiffness on RCC2 mRNA (normalized to GAPDH) and protein (normalized to β-actin) levels in A431 cells treated with vehicle (DMSO) or 5 μM Yoda1 to activate Piezo1 ( n = 3). (E) The effects of RCC2 overexpression on RCC2, KI67, PCNA, MMP2 and MMP9 protein (normalized to β-actin) levels in A431 cells ( n = 3). (F) The effects of RCC2 overexpression on YAP and TAZ mRNA levels (normalized to GAPDH) and YAP and p-YAP protein levels (normalized to β-actin) in A431 cells ( n = 3).

    Journal: Journal of Advanced Research

    Article Title: Matrix stiffness drives squamous cell carcinoma progression via a Piezo1-mediated mechanotransduction feedback loop

    doi: 10.1016/j.jare.2025.10.041

    Figure Lengend Snippet: RCC2 is essential to Piezo1-induced YAP activation in stiff matrix. (A) Uniform Manifold Approximation and Projection (UMAP) plots showing the major cell types in shNC ( n = 2) and shPiezo1 ( n = 2) cSCC tumor tissues. (B) Percentage and Ro/e of major cell types in shNC and shPiezo1 tumor tissue. (C) Four-quadrant plot displaying DEGs between shNC and shPiezo1 malignant cell and correlations between genes and Piezo1 to identified Piezo1-YAP regulators. (D) The effects of changing matrix stiffness on RCC2 mRNA (normalized to GAPDH) and protein (normalized to β-actin) levels in A431 cells treated with vehicle (DMSO) or 5 μM Yoda1 to activate Piezo1 ( n = 3). (E) The effects of RCC2 overexpression on RCC2, KI67, PCNA, MMP2 and MMP9 protein (normalized to β-actin) levels in A431 cells ( n = 3). (F) The effects of RCC2 overexpression on YAP and TAZ mRNA levels (normalized to GAPDH) and YAP and p-YAP protein levels (normalized to β-actin) in A431 cells ( n = 3).

    Article Snippet: GsMTx-4 (500 nM, Abcam) was used for inhibiting Piezo1, Yoda1 (5 μM, MedChemExpress) was applied for activating Piezo1, verteporfin (5 μM, Selleck) was applied for inhibiting YAP and PY-60 (10 μM, Selleck) was used for activating YAP.

    Techniques: Activation Assay, Over Expression

    ePLs modify dPIEZO activity in Drosophila cells (A–C) Representative Fura-2 imaging data for dPIEZO in control S2R+ cells and cells supplemented with 100 μM 18-AG. A, Typical ratiometric images before (Basal, at 60 s) and after the application of 10 μM Yoda1 (Yoda1, at 360 s) or 5 μM ionomycin (Ionomycin, at 540 s) in dPIEZO expressing cells (left) and dPIEZO expressing cells supplemented with 100 μM 18-AG (right). B, C, Representative Ca 2+ level traces in dPIEZO expressing cells (B), and 18-AG supplemented dPIEZO expressing cells (C). Red traces indicate Yoda1 responding cells (Δratio > 2). (D, E) Proportion of cells responding to Yoda1 (Δratio > 2) (D) and maximum Δratio response to Yoda1 normalized by the ionomycin response (E) in mock-transfected cells (control; n = 10, 18-AG; n = 10) and dPIEZO expressing cells (control, n = 10, 18-AG, n = 10). Each point represents a biological replicate; 25–40 cells were analyzed in each assay. Data are presented as mean ± SEM. ∗ p < 0.05 and ∗∗ p < 0.01; Tukey’s test. (F, G) Representative traces of patch-clamp recordings of mechanical stimuli-evoked dPIEZO activation without (F) or with (G) 100 μM 18-AG. (H) Quantification of the peak current density. Data are presented as mean ± SEM. ∗∗ p < 0.01; Mann-Whitney U test. (I) Proportion of responding cells (current size > 5 pA).

    Journal: iScience

    Article Title: Ether phospholipids modulate somatosensory responses by tuning multiple receptor functions in Drosophila

    doi: 10.1016/j.isci.2026.115209

    Figure Lengend Snippet: ePLs modify dPIEZO activity in Drosophila cells (A–C) Representative Fura-2 imaging data for dPIEZO in control S2R+ cells and cells supplemented with 100 μM 18-AG. A, Typical ratiometric images before (Basal, at 60 s) and after the application of 10 μM Yoda1 (Yoda1, at 360 s) or 5 μM ionomycin (Ionomycin, at 540 s) in dPIEZO expressing cells (left) and dPIEZO expressing cells supplemented with 100 μM 18-AG (right). B, C, Representative Ca 2+ level traces in dPIEZO expressing cells (B), and 18-AG supplemented dPIEZO expressing cells (C). Red traces indicate Yoda1 responding cells (Δratio > 2). (D, E) Proportion of cells responding to Yoda1 (Δratio > 2) (D) and maximum Δratio response to Yoda1 normalized by the ionomycin response (E) in mock-transfected cells (control; n = 10, 18-AG; n = 10) and dPIEZO expressing cells (control, n = 10, 18-AG, n = 10). Each point represents a biological replicate; 25–40 cells were analyzed in each assay. Data are presented as mean ± SEM. ∗ p < 0.05 and ∗∗ p < 0.01; Tukey’s test. (F, G) Representative traces of patch-clamp recordings of mechanical stimuli-evoked dPIEZO activation without (F) or with (G) 100 μM 18-AG. (H) Quantification of the peak current density. Data are presented as mean ± SEM. ∗∗ p < 0.01; Mann-Whitney U test. (I) Proportion of responding cells (current size > 5 pA).

    Article Snippet: Yoda1 , Tocris , 5586.

    Techniques: Activity Assay, Imaging, Control, Expressing, Transfection, Patch Clamp, Activation Assay, MANN-WHITNEY