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yeast1  (Millipore)


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    Structured Review

    Millipore yeast1
    Yeast1, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/yeast1/yeast+transformation+kit/pm37659077-290-30-28
    Average 90 stars, based on 1 article reviews
    yeast1 - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Transformation Assay:

    Article Title: Regulation of Rim4 distribution, function, and stability during meiosis by PKA, Cdc14, and 14-3-3 proteins.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Monoclonal anti-EGFP mouse IgG1k [7.1 and 13.1] (1:10,000) Roche Cat# 11814460001; RRID: AB_390913 Monoclonal anti-FLAG tag mouse IgG1 [M2] (1:5,000) Sigma-Aldrich Cat# F3165; RRID: AB_259529 Monoclonal anti-Phospho-PKA Substrate (RRxS*/T*) rabbit IgG [100G7E] (1:1,000) Cell Signaling Technologies Cat# 9624S; RRID: AB_331817 Recombinant anti-Thiophosphate ester (thioP) Rabbit IgG [51-8] (1:3,000) Abcam Cat# ab92570; RRID: AB_10562142 Recombinant Anti-Histone H2B (yeast Htb2) Rabbit IgG [EPR18094] (1:2,500) Abcam Cat# ab188291 Polyclonal anti-hexokinase 1 (Hxk1) Rabbit IgG (1:10,000) United States Biological Cat# 169073 Polyclonal anti-PGK1 Rabbit IgG (1:10,000) Antibodies Online Inc. Cat# ABIN568371; RRID: AB_2924789 StarBright B700 labeled goat antimouse IgG secondary antibody (1:10,000) Bio-Rad Cat# 12004158; RRID: AB_2884948 Alexa Fluor 488 labeled goat antirabbit IgG secondary (1:10,000) Invitrogen Cat# A11034; RRID: AB_2576217 ECL-anti-rabbit HRP IgG (1:10,000) Cytiva Cat# NA9340; RRID: AB_772191 Chemicals, peptides, and recombinant proteins SYTOX Green Thermo-Fisher Cat# S7020 b-estradiol Sigma-Aldrich Cat# E8875 1NM-PP1 APExBIO Cat# B1299 Ni-NTA agarose Qiagen Cat# 30230 Imidazole Fisher Scientific Cat# ICN1020335 Dynabeads Protein G Fisher Scientific Cat# 10004D PhosStop Roche Cat# 4906845001 Pepstatin Roche Cat# 11359053001 cOmplete Protease Inhibitor Cocktail Roche Cat# 11873580001 PMSF Sigma-Aldrich Cat# 78830 Bovine Serum Albumin Sigma-Aldrich Cat# A2153 4-20% CriterionTM TGX Stain-FreeTM Gel Bio-Rad Cat# 5678095 SurePAGETM 4–20% Bis-Tris Gel GenScript Cat# M00657 Anti-FLAG M2 Affinity Gel Sigma-Aldrich Cat# A2220 33FLAG peptides Sigma-Aldrich Cat# F4799 l-Phosphatase NEB Cat# P0753S N6-PhET-ATPgS Axxora Cat# BLG-P026-05 p-Nitrobenzyl mesylate (PNBM) Abcam Cat# Ab138910 Zymolyse (20T) AMS Bio Cat# 120491-1 Protein Kinase A Catalytic Subunit from bovine heart Sigma-Aldrich Cat# P2645 Rim4-6His This paper NA Rim4-GFP-6His This paper NA Rim4(5C)-6His This paper NA Rim4(PxLm)-6His This paper NA (Continued on next page) 18 Cell Reports 42, 113052, September 26, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Bmh1-GFP-6His This paper NA Bmh2-GFP-6His This paper NA Bmh1-3FLAG-6His This paper NA 6His-3FLAG-Cdc14 (Feng et al.)45 NA Critical commercial assays Yeast Transformation Kit Sigma-Aldrich Cat# YEAST1 GenCatchTM Plasmid DNA Mini-Prep Kit Epoch Cat# 2160250 GenCatchTM Gel Extraction Kit Epoch Cat# 2260250 Experimental models: Organisms/strains This paper Table S1 Recombinant DNA This paper Table S2 Software and algorithms Image LabTM (version 6.1) Bio-Rad https://www.bio-rad.com/en-us/product/ image-lab-software ImageJ (version 1.53u) National Institutes of Health (Public Domain) https://imagej-nih-gov.foyer.swmed.edu/ij/ SlideBook 6 Intelligent imaging https://www.intelligent-imaging.com/ slidebook Flowing Software (version 2.5.1) Turku Bioscience Center https://bioscience.fi/services/cell-imaging/ flowing-software/ SnapGene (version 7.0.2) Dotmatic https://www.snapgene.com/ GraphPad Prism (version 10.0.1) Dotmatic https://www.graphpad.com/ AlphaFold DeepMind Technologies https://alphafold.com/ Robetta Baker Lab, HHMI’s Janelia Research Campus http://www.robetta.org/ .. Further information and requests for resources and reagents should be directed to and will be fulfilled by the lead contact, Fei Wang (Fei.Wang@UTSouthwestern.edu).

    Plasmid Preparation:

    Article Title: Regulation of Rim4 distribution, function, and stability during meiosis by PKA, Cdc14, and 14-3-3 proteins.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Monoclonal anti-EGFP mouse IgG1k [7.1 and 13.1] (1:10,000) Roche Cat# 11814460001; RRID: AB_390913 Monoclonal anti-FLAG tag mouse IgG1 [M2] (1:5,000) Sigma-Aldrich Cat# F3165; RRID: AB_259529 Monoclonal anti-Phospho-PKA Substrate (RRxS*/T*) rabbit IgG [100G7E] (1:1,000) Cell Signaling Technologies Cat# 9624S; RRID: AB_331817 Recombinant anti-Thiophosphate ester (thioP) Rabbit IgG [51-8] (1:3,000) Abcam Cat# ab92570; RRID: AB_10562142 Recombinant Anti-Histone H2B (yeast Htb2) Rabbit IgG [EPR18094] (1:2,500) Abcam Cat# ab188291 Polyclonal anti-hexokinase 1 (Hxk1) Rabbit IgG (1:10,000) United States Biological Cat# 169073 Polyclonal anti-PGK1 Rabbit IgG (1:10,000) Antibodies Online Inc. Cat# ABIN568371; RRID: AB_2924789 StarBright B700 labeled goat antimouse IgG secondary antibody (1:10,000) Bio-Rad Cat# 12004158; RRID: AB_2884948 Alexa Fluor 488 labeled goat antirabbit IgG secondary (1:10,000) Invitrogen Cat# A11034; RRID: AB_2576217 ECL-anti-rabbit HRP IgG (1:10,000) Cytiva Cat# NA9340; RRID: AB_772191 Chemicals, peptides, and recombinant proteins SYTOX Green Thermo-Fisher Cat# S7020 b-estradiol Sigma-Aldrich Cat# E8875 1NM-PP1 APExBIO Cat# B1299 Ni-NTA agarose Qiagen Cat# 30230 Imidazole Fisher Scientific Cat# ICN1020335 Dynabeads Protein G Fisher Scientific Cat# 10004D PhosStop Roche Cat# 4906845001 Pepstatin Roche Cat# 11359053001 cOmplete Protease Inhibitor Cocktail Roche Cat# 11873580001 PMSF Sigma-Aldrich Cat# 78830 Bovine Serum Albumin Sigma-Aldrich Cat# A2153 4-20% CriterionTM TGX Stain-FreeTM Gel Bio-Rad Cat# 5678095 SurePAGETM 4–20% Bis-Tris Gel GenScript Cat# M00657 Anti-FLAG M2 Affinity Gel Sigma-Aldrich Cat# A2220 33FLAG peptides Sigma-Aldrich Cat# F4799 l-Phosphatase NEB Cat# P0753S N6-PhET-ATPgS Axxora Cat# BLG-P026-05 p-Nitrobenzyl mesylate (PNBM) Abcam Cat# Ab138910 Zymolyse (20T) AMS Bio Cat# 120491-1 Protein Kinase A Catalytic Subunit from bovine heart Sigma-Aldrich Cat# P2645 Rim4-6His This paper NA Rim4-GFP-6His This paper NA Rim4(5C)-6His This paper NA Rim4(PxLm)-6His This paper NA (Continued on next page) 18 Cell Reports 42, 113052, September 26, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Bmh1-GFP-6His This paper NA Bmh2-GFP-6His This paper NA Bmh1-3FLAG-6His This paper NA 6His-3FLAG-Cdc14 (Feng et al.)45 NA Critical commercial assays Yeast Transformation Kit Sigma-Aldrich Cat# YEAST1 GenCatchTM Plasmid DNA Mini-Prep Kit Epoch Cat# 2160250 GenCatchTM Gel Extraction Kit Epoch Cat# 2260250 Experimental models: Organisms/strains This paper Table S1 Recombinant DNA This paper Table S2 Software and algorithms Image LabTM (version 6.1) Bio-Rad https://www.bio-rad.com/en-us/product/ image-lab-software ImageJ (version 1.53u) National Institutes of Health (Public Domain) https://imagej-nih-gov.foyer.swmed.edu/ij/ SlideBook 6 Intelligent imaging https://www.intelligent-imaging.com/ slidebook Flowing Software (version 2.5.1) Turku Bioscience Center https://bioscience.fi/services/cell-imaging/ flowing-software/ SnapGene (version 7.0.2) Dotmatic https://www.snapgene.com/ GraphPad Prism (version 10.0.1) Dotmatic https://www.graphpad.com/ AlphaFold DeepMind Technologies https://alphafold.com/ Robetta Baker Lab, HHMI’s Janelia Research Campus http://www.robetta.org/ .. Further information and requests for resources and reagents should be directed to and will be fulfilled by the lead contact, Fei Wang (Fei.Wang@UTSouthwestern.edu).

    Gel Extraction:

    Article Title: Regulation of Rim4 distribution, function, and stability during meiosis by PKA, Cdc14, and 14-3-3 proteins.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Monoclonal anti-EGFP mouse IgG1k [7.1 and 13.1] (1:10,000) Roche Cat# 11814460001; RRID: AB_390913 Monoclonal anti-FLAG tag mouse IgG1 [M2] (1:5,000) Sigma-Aldrich Cat# F3165; RRID: AB_259529 Monoclonal anti-Phospho-PKA Substrate (RRxS*/T*) rabbit IgG [100G7E] (1:1,000) Cell Signaling Technologies Cat# 9624S; RRID: AB_331817 Recombinant anti-Thiophosphate ester (thioP) Rabbit IgG [51-8] (1:3,000) Abcam Cat# ab92570; RRID: AB_10562142 Recombinant Anti-Histone H2B (yeast Htb2) Rabbit IgG [EPR18094] (1:2,500) Abcam Cat# ab188291 Polyclonal anti-hexokinase 1 (Hxk1) Rabbit IgG (1:10,000) United States Biological Cat# 169073 Polyclonal anti-PGK1 Rabbit IgG (1:10,000) Antibodies Online Inc. Cat# ABIN568371; RRID: AB_2924789 StarBright B700 labeled goat antimouse IgG secondary antibody (1:10,000) Bio-Rad Cat# 12004158; RRID: AB_2884948 Alexa Fluor 488 labeled goat antirabbit IgG secondary (1:10,000) Invitrogen Cat# A11034; RRID: AB_2576217 ECL-anti-rabbit HRP IgG (1:10,000) Cytiva Cat# NA9340; RRID: AB_772191 Chemicals, peptides, and recombinant proteins SYTOX Green Thermo-Fisher Cat# S7020 b-estradiol Sigma-Aldrich Cat# E8875 1NM-PP1 APExBIO Cat# B1299 Ni-NTA agarose Qiagen Cat# 30230 Imidazole Fisher Scientific Cat# ICN1020335 Dynabeads Protein G Fisher Scientific Cat# 10004D PhosStop Roche Cat# 4906845001 Pepstatin Roche Cat# 11359053001 cOmplete Protease Inhibitor Cocktail Roche Cat# 11873580001 PMSF Sigma-Aldrich Cat# 78830 Bovine Serum Albumin Sigma-Aldrich Cat# A2153 4-20% CriterionTM TGX Stain-FreeTM Gel Bio-Rad Cat# 5678095 SurePAGETM 4–20% Bis-Tris Gel GenScript Cat# M00657 Anti-FLAG M2 Affinity Gel Sigma-Aldrich Cat# A2220 33FLAG peptides Sigma-Aldrich Cat# F4799 l-Phosphatase NEB Cat# P0753S N6-PhET-ATPgS Axxora Cat# BLG-P026-05 p-Nitrobenzyl mesylate (PNBM) Abcam Cat# Ab138910 Zymolyse (20T) AMS Bio Cat# 120491-1 Protein Kinase A Catalytic Subunit from bovine heart Sigma-Aldrich Cat# P2645 Rim4-6His This paper NA Rim4-GFP-6His This paper NA Rim4(5C)-6His This paper NA Rim4(PxLm)-6His This paper NA (Continued on next page) 18 Cell Reports 42, 113052, September 26, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Bmh1-GFP-6His This paper NA Bmh2-GFP-6His This paper NA Bmh1-3FLAG-6His This paper NA 6His-3FLAG-Cdc14 (Feng et al.)45 NA Critical commercial assays Yeast Transformation Kit Sigma-Aldrich Cat# YEAST1 GenCatchTM Plasmid DNA Mini-Prep Kit Epoch Cat# 2160250 GenCatchTM Gel Extraction Kit Epoch Cat# 2260250 Experimental models: Organisms/strains This paper Table S1 Recombinant DNA This paper Table S2 Software and algorithms Image LabTM (version 6.1) Bio-Rad https://www.bio-rad.com/en-us/product/ image-lab-software ImageJ (version 1.53u) National Institutes of Health (Public Domain) https://imagej-nih-gov.foyer.swmed.edu/ij/ SlideBook 6 Intelligent imaging https://www.intelligent-imaging.com/ slidebook Flowing Software (version 2.5.1) Turku Bioscience Center https://bioscience.fi/services/cell-imaging/ flowing-software/ SnapGene (version 7.0.2) Dotmatic https://www.snapgene.com/ GraphPad Prism (version 10.0.1) Dotmatic https://www.graphpad.com/ AlphaFold DeepMind Technologies https://alphafold.com/ Robetta Baker Lab, HHMI’s Janelia Research Campus http://www.robetta.org/ .. Further information and requests for resources and reagents should be directed to and will be fulfilled by the lead contact, Fei Wang (Fei.Wang@UTSouthwestern.edu).

    Recombinant:

    Article Title: Regulation of Rim4 distribution, function, and stability during meiosis by PKA, Cdc14, and 14-3-3 proteins.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Monoclonal anti-EGFP mouse IgG1k [7.1 and 13.1] (1:10,000) Roche Cat# 11814460001; RRID: AB_390913 Monoclonal anti-FLAG tag mouse IgG1 [M2] (1:5,000) Sigma-Aldrich Cat# F3165; RRID: AB_259529 Monoclonal anti-Phospho-PKA Substrate (RRxS*/T*) rabbit IgG [100G7E] (1:1,000) Cell Signaling Technologies Cat# 9624S; RRID: AB_331817 Recombinant anti-Thiophosphate ester (thioP) Rabbit IgG [51-8] (1:3,000) Abcam Cat# ab92570; RRID: AB_10562142 Recombinant Anti-Histone H2B (yeast Htb2) Rabbit IgG [EPR18094] (1:2,500) Abcam Cat# ab188291 Polyclonal anti-hexokinase 1 (Hxk1) Rabbit IgG (1:10,000) United States Biological Cat# 169073 Polyclonal anti-PGK1 Rabbit IgG (1:10,000) Antibodies Online Inc. Cat# ABIN568371; RRID: AB_2924789 StarBright B700 labeled goat antimouse IgG secondary antibody (1:10,000) Bio-Rad Cat# 12004158; RRID: AB_2884948 Alexa Fluor 488 labeled goat antirabbit IgG secondary (1:10,000) Invitrogen Cat# A11034; RRID: AB_2576217 ECL-anti-rabbit HRP IgG (1:10,000) Cytiva Cat# NA9340; RRID: AB_772191 Chemicals, peptides, and recombinant proteins SYTOX Green Thermo-Fisher Cat# S7020 b-estradiol Sigma-Aldrich Cat# E8875 1NM-PP1 APExBIO Cat# B1299 Ni-NTA agarose Qiagen Cat# 30230 Imidazole Fisher Scientific Cat# ICN1020335 Dynabeads Protein G Fisher Scientific Cat# 10004D PhosStop Roche Cat# 4906845001 Pepstatin Roche Cat# 11359053001 cOmplete Protease Inhibitor Cocktail Roche Cat# 11873580001 PMSF Sigma-Aldrich Cat# 78830 Bovine Serum Albumin Sigma-Aldrich Cat# A2153 4-20% CriterionTM TGX Stain-FreeTM Gel Bio-Rad Cat# 5678095 SurePAGETM 4–20% Bis-Tris Gel GenScript Cat# M00657 Anti-FLAG M2 Affinity Gel Sigma-Aldrich Cat# A2220 33FLAG peptides Sigma-Aldrich Cat# F4799 l-Phosphatase NEB Cat# P0753S N6-PhET-ATPgS Axxora Cat# BLG-P026-05 p-Nitrobenzyl mesylate (PNBM) Abcam Cat# Ab138910 Zymolyse (20T) AMS Bio Cat# 120491-1 Protein Kinase A Catalytic Subunit from bovine heart Sigma-Aldrich Cat# P2645 Rim4-6His This paper NA Rim4-GFP-6His This paper NA Rim4(5C)-6His This paper NA Rim4(PxLm)-6His This paper NA (Continued on next page) 18 Cell Reports 42, 113052, September 26, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Bmh1-GFP-6His This paper NA Bmh2-GFP-6His This paper NA Bmh1-3FLAG-6His This paper NA 6His-3FLAG-Cdc14 (Feng et al.)45 NA Critical commercial assays Yeast Transformation Kit Sigma-Aldrich Cat# YEAST1 GenCatchTM Plasmid DNA Mini-Prep Kit Epoch Cat# 2160250 GenCatchTM Gel Extraction Kit Epoch Cat# 2260250 Experimental models: Organisms/strains This paper Table S1 Recombinant DNA This paper Table S2 Software and algorithms Image LabTM (version 6.1) Bio-Rad https://www.bio-rad.com/en-us/product/ image-lab-software ImageJ (version 1.53u) National Institutes of Health (Public Domain) https://imagej-nih-gov.foyer.swmed.edu/ij/ SlideBook 6 Intelligent imaging https://www.intelligent-imaging.com/ slidebook Flowing Software (version 2.5.1) Turku Bioscience Center https://bioscience.fi/services/cell-imaging/ flowing-software/ SnapGene (version 7.0.2) Dotmatic https://www.snapgene.com/ GraphPad Prism (version 10.0.1) Dotmatic https://www.graphpad.com/ AlphaFold DeepMind Technologies https://alphafold.com/ Robetta Baker Lab, HHMI’s Janelia Research Campus http://www.robetta.org/ .. Further information and requests for resources and reagents should be directed to and will be fulfilled by the lead contact, Fei Wang (Fei.Wang@UTSouthwestern.edu).

    Software:

    Article Title: Regulation of Rim4 distribution, function, and stability during meiosis by PKA, Cdc14, and 14-3-3 proteins.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Monoclonal anti-EGFP mouse IgG1k [7.1 and 13.1] (1:10,000) Roche Cat# 11814460001; RRID: AB_390913 Monoclonal anti-FLAG tag mouse IgG1 [M2] (1:5,000) Sigma-Aldrich Cat# F3165; RRID: AB_259529 Monoclonal anti-Phospho-PKA Substrate (RRxS*/T*) rabbit IgG [100G7E] (1:1,000) Cell Signaling Technologies Cat# 9624S; RRID: AB_331817 Recombinant anti-Thiophosphate ester (thioP) Rabbit IgG [51-8] (1:3,000) Abcam Cat# ab92570; RRID: AB_10562142 Recombinant Anti-Histone H2B (yeast Htb2) Rabbit IgG [EPR18094] (1:2,500) Abcam Cat# ab188291 Polyclonal anti-hexokinase 1 (Hxk1) Rabbit IgG (1:10,000) United States Biological Cat# 169073 Polyclonal anti-PGK1 Rabbit IgG (1:10,000) Antibodies Online Inc. Cat# ABIN568371; RRID: AB_2924789 StarBright B700 labeled goat antimouse IgG secondary antibody (1:10,000) Bio-Rad Cat# 12004158; RRID: AB_2884948 Alexa Fluor 488 labeled goat antirabbit IgG secondary (1:10,000) Invitrogen Cat# A11034; RRID: AB_2576217 ECL-anti-rabbit HRP IgG (1:10,000) Cytiva Cat# NA9340; RRID: AB_772191 Chemicals, peptides, and recombinant proteins SYTOX Green Thermo-Fisher Cat# S7020 b-estradiol Sigma-Aldrich Cat# E8875 1NM-PP1 APExBIO Cat# B1299 Ni-NTA agarose Qiagen Cat# 30230 Imidazole Fisher Scientific Cat# ICN1020335 Dynabeads Protein G Fisher Scientific Cat# 10004D PhosStop Roche Cat# 4906845001 Pepstatin Roche Cat# 11359053001 cOmplete Protease Inhibitor Cocktail Roche Cat# 11873580001 PMSF Sigma-Aldrich Cat# 78830 Bovine Serum Albumin Sigma-Aldrich Cat# A2153 4-20% CriterionTM TGX Stain-FreeTM Gel Bio-Rad Cat# 5678095 SurePAGETM 4–20% Bis-Tris Gel GenScript Cat# M00657 Anti-FLAG M2 Affinity Gel Sigma-Aldrich Cat# A2220 33FLAG peptides Sigma-Aldrich Cat# F4799 l-Phosphatase NEB Cat# P0753S N6-PhET-ATPgS Axxora Cat# BLG-P026-05 p-Nitrobenzyl mesylate (PNBM) Abcam Cat# Ab138910 Zymolyse (20T) AMS Bio Cat# 120491-1 Protein Kinase A Catalytic Subunit from bovine heart Sigma-Aldrich Cat# P2645 Rim4-6His This paper NA Rim4-GFP-6His This paper NA Rim4(5C)-6His This paper NA Rim4(PxLm)-6His This paper NA (Continued on next page) 18 Cell Reports 42, 113052, September 26, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Bmh1-GFP-6His This paper NA Bmh2-GFP-6His This paper NA Bmh1-3FLAG-6His This paper NA 6His-3FLAG-Cdc14 (Feng et al.)45 NA Critical commercial assays Yeast Transformation Kit Sigma-Aldrich Cat# YEAST1 GenCatchTM Plasmid DNA Mini-Prep Kit Epoch Cat# 2160250 GenCatchTM Gel Extraction Kit Epoch Cat# 2260250 Experimental models: Organisms/strains This paper Table S1 Recombinant DNA This paper Table S2 Software and algorithms Image LabTM (version 6.1) Bio-Rad https://www.bio-rad.com/en-us/product/ image-lab-software ImageJ (version 1.53u) National Institutes of Health (Public Domain) https://imagej-nih-gov.foyer.swmed.edu/ij/ SlideBook 6 Intelligent imaging https://www.intelligent-imaging.com/ slidebook Flowing Software (version 2.5.1) Turku Bioscience Center https://bioscience.fi/services/cell-imaging/ flowing-software/ SnapGene (version 7.0.2) Dotmatic https://www.snapgene.com/ GraphPad Prism (version 10.0.1) Dotmatic https://www.graphpad.com/ AlphaFold DeepMind Technologies https://alphafold.com/ Robetta Baker Lab, HHMI’s Janelia Research Campus http://www.robetta.org/ .. Further information and requests for resources and reagents should be directed to and will be fulfilled by the lead contact, Fei Wang (Fei.Wang@UTSouthwestern.edu).

    Imaging:

    Article Title: Regulation of Rim4 distribution, function, and stability during meiosis by PKA, Cdc14, and 14-3-3 proteins.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Monoclonal anti-EGFP mouse IgG1k [7.1 and 13.1] (1:10,000) Roche Cat# 11814460001; RRID: AB_390913 Monoclonal anti-FLAG tag mouse IgG1 [M2] (1:5,000) Sigma-Aldrich Cat# F3165; RRID: AB_259529 Monoclonal anti-Phospho-PKA Substrate (RRxS*/T*) rabbit IgG [100G7E] (1:1,000) Cell Signaling Technologies Cat# 9624S; RRID: AB_331817 Recombinant anti-Thiophosphate ester (thioP) Rabbit IgG [51-8] (1:3,000) Abcam Cat# ab92570; RRID: AB_10562142 Recombinant Anti-Histone H2B (yeast Htb2) Rabbit IgG [EPR18094] (1:2,500) Abcam Cat# ab188291 Polyclonal anti-hexokinase 1 (Hxk1) Rabbit IgG (1:10,000) United States Biological Cat# 169073 Polyclonal anti-PGK1 Rabbit IgG (1:10,000) Antibodies Online Inc. Cat# ABIN568371; RRID: AB_2924789 StarBright B700 labeled goat antimouse IgG secondary antibody (1:10,000) Bio-Rad Cat# 12004158; RRID: AB_2884948 Alexa Fluor 488 labeled goat antirabbit IgG secondary (1:10,000) Invitrogen Cat# A11034; RRID: AB_2576217 ECL-anti-rabbit HRP IgG (1:10,000) Cytiva Cat# NA9340; RRID: AB_772191 Chemicals, peptides, and recombinant proteins SYTOX Green Thermo-Fisher Cat# S7020 b-estradiol Sigma-Aldrich Cat# E8875 1NM-PP1 APExBIO Cat# B1299 Ni-NTA agarose Qiagen Cat# 30230 Imidazole Fisher Scientific Cat# ICN1020335 Dynabeads Protein G Fisher Scientific Cat# 10004D PhosStop Roche Cat# 4906845001 Pepstatin Roche Cat# 11359053001 cOmplete Protease Inhibitor Cocktail Roche Cat# 11873580001 PMSF Sigma-Aldrich Cat# 78830 Bovine Serum Albumin Sigma-Aldrich Cat# A2153 4-20% CriterionTM TGX Stain-FreeTM Gel Bio-Rad Cat# 5678095 SurePAGETM 4–20% Bis-Tris Gel GenScript Cat# M00657 Anti-FLAG M2 Affinity Gel Sigma-Aldrich Cat# A2220 33FLAG peptides Sigma-Aldrich Cat# F4799 l-Phosphatase NEB Cat# P0753S N6-PhET-ATPgS Axxora Cat# BLG-P026-05 p-Nitrobenzyl mesylate (PNBM) Abcam Cat# Ab138910 Zymolyse (20T) AMS Bio Cat# 120491-1 Protein Kinase A Catalytic Subunit from bovine heart Sigma-Aldrich Cat# P2645 Rim4-6His This paper NA Rim4-GFP-6His This paper NA Rim4(5C)-6His This paper NA Rim4(PxLm)-6His This paper NA (Continued on next page) 18 Cell Reports 42, 113052, September 26, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Bmh1-GFP-6His This paper NA Bmh2-GFP-6His This paper NA Bmh1-3FLAG-6His This paper NA 6His-3FLAG-Cdc14 (Feng et al.)45 NA Critical commercial assays Yeast Transformation Kit Sigma-Aldrich Cat# YEAST1 GenCatchTM Plasmid DNA Mini-Prep Kit Epoch Cat# 2160250 GenCatchTM Gel Extraction Kit Epoch Cat# 2260250 Experimental models: Organisms/strains This paper Table S1 Recombinant DNA This paper Table S2 Software and algorithms Image LabTM (version 6.1) Bio-Rad https://www.bio-rad.com/en-us/product/ image-lab-software ImageJ (version 1.53u) National Institutes of Health (Public Domain) https://imagej-nih-gov.foyer.swmed.edu/ij/ SlideBook 6 Intelligent imaging https://www.intelligent-imaging.com/ slidebook Flowing Software (version 2.5.1) Turku Bioscience Center https://bioscience.fi/services/cell-imaging/ flowing-software/ SnapGene (version 7.0.2) Dotmatic https://www.snapgene.com/ GraphPad Prism (version 10.0.1) Dotmatic https://www.graphpad.com/ AlphaFold DeepMind Technologies https://alphafold.com/ Robetta Baker Lab, HHMI’s Janelia Research Campus http://www.robetta.org/ .. Further information and requests for resources and reagents should be directed to and will be fulfilled by the lead contact, Fei Wang (Fei.Wang@UTSouthwestern.edu).



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