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yap1 phosphorylated on serine 127  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc yap1 phosphorylated on serine 127
    Alterations in mammary tumors upon Dnph1 knockout (A) Examples of CD31 staining in mammary tumors from a MMTV-Her2/Dnph1 +/+ and a MMTV-Her2/Dnph1 −/− mouse. Right panels show ImageJ-mediated image modulation to enhance vessel structures, allowing the computer-mediated quantification of blood vessel density. Scale bars, 0.4 mm. (B) Corresponding quantification of vessel density. Tumors were age- and weight-matched; two-tailed, unpaired t test. (C) Tumor vessel density plotted against the weight of the tumor. These tumors were not weight- or age-matched, yet include those shown in panel B. Shown are linear regressions; while the slopes were not significantly different, the Y-intercepts were significantly different ( p = 0.0007). (D) Relative levels of AMP, GMP, and UMP in mammary tumors from MMTV-Her2/Dnph1 +/+ and MMTV-Her2/Dnph1 −/− mice; two-tailed, unpaired t test. (E and F) Immunohistochemical staining for phospho-AMPKα or <t>phospho-YAP1,</t> respectively, on age- and weight-matched tumors as in panel B. Examples of immunohistochemical staining are shown at the bottom. Scale bars, 0.4 mm. Means with standard deviation are shown in panels B and D-F.
    Yap1 Phosphorylated On Serine 127, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 913 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/yap1/Phospho-YAP+(Ser127)+Antibody/pmc12990376-7-0-6
    Average 96 stars, based on 913 article reviews
    yap1 phosphorylated on serine 127 - by Bioz Stars, 2026-09
    96/100 stars

    Images

    1) Product Images from "Promotion of breast cancer by the DNPH1 enzyme"

    Article Title: Promotion of breast cancer by the DNPH1 enzyme

    Journal: iScience

    doi: 10.1016/j.isci.2026.115227

    Alterations in mammary tumors upon Dnph1 knockout (A) Examples of CD31 staining in mammary tumors from a MMTV-Her2/Dnph1 +/+ and a MMTV-Her2/Dnph1 −/− mouse. Right panels show ImageJ-mediated image modulation to enhance vessel structures, allowing the computer-mediated quantification of blood vessel density. Scale bars, 0.4 mm. (B) Corresponding quantification of vessel density. Tumors were age- and weight-matched; two-tailed, unpaired t test. (C) Tumor vessel density plotted against the weight of the tumor. These tumors were not weight- or age-matched, yet include those shown in panel B. Shown are linear regressions; while the slopes were not significantly different, the Y-intercepts were significantly different ( p = 0.0007). (D) Relative levels of AMP, GMP, and UMP in mammary tumors from MMTV-Her2/Dnph1 +/+ and MMTV-Her2/Dnph1 −/− mice; two-tailed, unpaired t test. (E and F) Immunohistochemical staining for phospho-AMPKα or phospho-YAP1, respectively, on age- and weight-matched tumors as in panel B. Examples of immunohistochemical staining are shown at the bottom. Scale bars, 0.4 mm. Means with standard deviation are shown in panels B and D-F.
    Figure Legend Snippet: Alterations in mammary tumors upon Dnph1 knockout (A) Examples of CD31 staining in mammary tumors from a MMTV-Her2/Dnph1 +/+ and a MMTV-Her2/Dnph1 −/− mouse. Right panels show ImageJ-mediated image modulation to enhance vessel structures, allowing the computer-mediated quantification of blood vessel density. Scale bars, 0.4 mm. (B) Corresponding quantification of vessel density. Tumors were age- and weight-matched; two-tailed, unpaired t test. (C) Tumor vessel density plotted against the weight of the tumor. These tumors were not weight- or age-matched, yet include those shown in panel B. Shown are linear regressions; while the slopes were not significantly different, the Y-intercepts were significantly different ( p = 0.0007). (D) Relative levels of AMP, GMP, and UMP in mammary tumors from MMTV-Her2/Dnph1 +/+ and MMTV-Her2/Dnph1 −/− mice; two-tailed, unpaired t test. (E and F) Immunohistochemical staining for phospho-AMPKα or phospho-YAP1, respectively, on age- and weight-matched tumors as in panel B. Examples of immunohistochemical staining are shown at the bottom. Scale bars, 0.4 mm. Means with standard deviation are shown in panels B and D-F.

    Techniques Used: Knock-Out, Staining, Two Tailed Test, Immunohistochemical staining, Standard Deviation

    Model how DNPH1 promotes breast tumor development and progression This model is centered around a DNPH1-YAP1 axis, yet DNPH1 may utilize to-be-identified effectors other than YAP1 to stimulate, e.g., stem cells or angiogenesis.
    Figure Legend Snippet: Model how DNPH1 promotes breast tumor development and progression This model is centered around a DNPH1-YAP1 axis, yet DNPH1 may utilize to-be-identified effectors other than YAP1 to stimulate, e.g., stem cells or angiogenesis.

    Techniques Used:

    Related Articles

    Immunohistochemistry:

    Article Title: YAP1 Dysfunction Promotes Molecular Properties Linked to Breast Cancer Susceptibility
    Article Snippet: .. YAP1 (clone #D8H1X, cat. #14074, Cell Signaling Technology) IHC was performed on a Ventana DISCOVERY ULTRA IHC automated stainer (Ventana Medical Systems, Roche Diagnostics). ..

    Control:

    Article Title: Af-CUT&Tag: a sensitive and antibody-free chromatin profiling method using genetically encoded tags and high-affinity binders fused to Tn5
    Article Snippet: Human RKO (Cat#TCHu116), DLD1 (Cat#CC0507), and SW480 (Cat#CCL-228) cell lines were obtained from the Shanghai Institute of Biochemistry and Cell Biology, Guangzhou Cellcook Biotech Company, and the American Type Culture Collection (ATCC), respectively, and cultured according to the suppliers’ instructions. .. We used the following antibodies: Rabbit Control IgG (Abclonal, AC005), Phospho-Rpb1 CTD (Ser2) (Cell Signaling Technology, 13499), CTCF (Cell Signaling Technology, 3418), YAP1 (Cell Signaling Technology, 14074), TAZ (Cell Signaling Technology, 72804), HiBiT (Promega, N7200), Histone H3 (acetyl K27) (Abcam, ab4729), Ki-67 (Abcam, ab279653), PCNA (Santa Cruz, sc-56), Goat anti-rabbit secondary antibody (Thermo Fisher Scientific, 31210), and Goat anti-mouse secondary antibody (Thermo Fisher Scientific, 31160). .. C57BL/6JGpt-Rosa26 tm1(CAG-LSL-Cas9-tdTomato) /Gpt mice were purchased from GemPharmatech (T002249).

    Article Title: A Three-subtype Molecular model of Cervical Cancer: Multiple PI3K Pathway inhibitors suppress growth and cooperate with HPV-directed immunotherapy
    Article Snippet: In total, 20 - 40 micrograms of whole cell protein were separated in a 4–12% NuPAGE Bis-Tris-gel, transferred to PVDF membrane (Thermo Fisher Scientific, Carlsbad, CA, United States), and immunoblotted with primary antibodies in 0.05% Tween 20-Tris-buffered saline (TBST) containing 5% skim milk at 4°C with shaking overnight. .. The primary antibodies used in this study were: rabbit monoclonal anti-PD-L1, (Cell Signaling Technology Cat# 13684, RRID: AB_2687655), YAP1, (Cell Signaling Technology Cat# 14074, RRID: AB_2650491), EGFR (Cell Signaling Technology Cat# 4267, RRID: AB_2800085), CTGF (Cell Signaling Technology Cat# 86641, RRID: AB_2800085), Integrin α6 (Cell Signaling Technology Cat# 3750, RRID: AB_2249263), IRF1 (Cell Signaling Technology Cat# 8478, RRID: AB_10949108) at a dilution of 1:500; mouse monoclonal anti-HPV16 E7 at 1:100 (Santa Cruz Cat# sc-51951, RRID: AB_629662), and goat monoclonal anti-p16 at 1:500 (R&D system Cat# AF5779, RRID: AB_1964666). β-actin, used as an internal control, was detected by rabbit anti-β-actin antibody (Cell Signaling Technology Cat# 4967, RRID: AB_330288). .. Anti-rabbit IgG, HRP-linked Antibody (Cell Signaling Technology Cat# 7074, RRID: AB_2099233) and Anti-mouse IgG, HRP-linked Antibody at 1:3000 - 1:5000 (Cell Signaling Technology Cat# 7076, RRID: AB_330924) were used as a secondary antibody.

    Incubation:

    Article Title: TROP2 confers resistance to oxidative stress-induced cancer cell death through YAP/HMOX1 signaling
    Article Snippet: Total protein (20-30ug/sample) was separated on SDS-PAGE (10%) and transferred to a PVDF membrane (Bio-Rad, Hercules, CA, USA). .. After that, the membranes were blocked in 5% fat-free milk for 1 h. The membranes were incubated with the primary antibodies including TROP2 (1:1000, cat #47866, CST), GPX4 (1:1000, cat #AF7020, Beyotime), SLC7A11 (1:1000, cat #AF7992, Beyotime), ACSL4 (1:1000, cat #AG1908, Beyotime), HMOX1 (1:1000, cat #AG2181, Beyotime), YAP1 (1:1000, cat #14074, CST), P-YAP1 (1:1000, cat #13619, CST), LATS1 (1:1000, cat #3477, CST), p-LATS1 (1:1000, cat #9157, CST), Histone3 (1:1000, cat #9715, CST),α-Tubulin (1:1000, cat #2144, CST) at 4 °C overnight and the secondary antibodies at room temperature for 2 h. Protein bands were visualized using the ECL (Bio-Rad, Hercules, CA, USA). .. CCK8 assay (Beyotime Biotechnology, Shanghai, China) was used for cell survival detection based on the manufacturer’s manual.

    Article Title: TROP2 confers resistance to oxidative stress-induced cancer cell death through YAP/HMOX1 signaling.
    Article Snippet: .. The membranes were incubated with the primary antibodies including TROP2 (1:1000, cat #47866, CST), GPX4 (1:1000, cat #AF7020, Beyotime), SLC7A11 (1:1000, cat #AF7992, Beyotime), ACSL4 (1:1000, cat #AG1908, Beyotime), HMOX1 (1:1000, cat #AG2181, Beyotime), YAP1 (1:1000, cat #14074, CST), P-YAP1 (1:1000, cat #13619, CST), LATS1 (1:1000, cat #3477, CST), p-LATS1 (1:1000, cat #9157, CST), Histone3 (1:1000, cat #9715, CST),α-Tubulin (1:1000, cat #2144, CST) at 4oC overnight and the secondary antibodies at room temperature for 2h. .. Protein bands were visualized using the ECL (Bio-Rad, Hercules, CA, USA).

    other:


    Isolation:

    Article Title: Co-repression of Yap1 and Sox9 Abrogates Established Cholangiocarcinoma by Eliminating Transcriptional Compensation.
    Article Snippet: An aliquot of chromatin (40 ug) was precleared with protein A agarose beads (Invitrogen, CA). .. Genomic DNA regions of interest were isolated using antibodies against SOX9 (Millipore Sigma# AB5535), YAP1 (Cell Signaling# 14074S), TEAD1 (Santa Cruz# sc-101184) and TAZ (Millipore Sigma# HPA007415). ..



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    Alterations in mammary tumors upon Dnph1 knockout (A) Examples of CD31 staining in mammary tumors from a MMTV-Her2/Dnph1 +/+ and a MMTV-Her2/Dnph1 −/− mouse. Right panels show ImageJ-mediated image modulation to enhance vessel structures, allowing the computer-mediated quantification of blood vessel density. Scale bars, 0.4 mm. (B) Corresponding quantification of vessel density. Tumors were age- and weight-matched; two-tailed, unpaired t test. (C) Tumor vessel density plotted against the weight of the tumor. These tumors were not weight- or age-matched, yet include those shown in panel B. Shown are linear regressions; while the slopes were not significantly different, the Y-intercepts were significantly different ( p = 0.0007). (D) Relative levels of AMP, GMP, and UMP in mammary tumors from MMTV-Her2/Dnph1 +/+ and MMTV-Her2/Dnph1 −/− mice; two-tailed, unpaired t test. (E and F) Immunohistochemical staining for phospho-AMPKα or <t>phospho-YAP1,</t> respectively, on age- and weight-matched tumors as in panel B. Examples of immunohistochemical staining are shown at the bottom. Scale bars, 0.4 mm. Means with standard deviation are shown in panels B and D-F.
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    Image Search Results


    ( A )YAP1 protein is expressed in different cell types relevant to the brain, such as astrocytes (CCF-STTG1), microglia (HMC3), neuroblastoma (SH-SY5Y), and NT2 cells, as demonstrated by immunoblotting. Data are mean ± SEM ( n = 3). ( B ) Images show immunocytochemical evidence of YAP1 expression (in green) and DAPI-stained nuclei (in blue) in different cell types and human neurons. Scale: 20 µm.

    Journal: Neurology International

    Article Title: YAP1 Upregulates Cytoskeleton Regulator ARHGEF1 and Tissue Regeneration Factor NEDD9 in a Multiplex Proteomic Study

    doi: 10.3390/neurolint18050096

    Figure Lengend Snippet: ( A )YAP1 protein is expressed in different cell types relevant to the brain, such as astrocytes (CCF-STTG1), microglia (HMC3), neuroblastoma (SH-SY5Y), and NT2 cells, as demonstrated by immunoblotting. Data are mean ± SEM ( n = 3). ( B ) Images show immunocytochemical evidence of YAP1 expression (in green) and DAPI-stained nuclei (in blue) in different cell types and human neurons. Scale: 20 µm.

    Article Snippet: We cultured the immortalized human neuroblastoma cell line SH-SY5Y (cat # CRL2266, ATCC) in DMEM/F12 medium and infected only once with Lenti ORF particles, human YAP1 (mGFP-tagged) transcript variant 1 (cat # RC225864L4V, Origene, Rockville, MD, USA), and generated stable cells expressing YAP1-GFP with puromycin selection at 5.0 μg per milliliter for two weeks.

    Techniques: Western Blot, Expressing, Staining

    YAP1 protein levels are significantly downregulated in the nuclear fractions but not the cytosolic fractions of the AD brains relative to NC brains. Brain lysates were immunoblotted, and protein levels were quantified by normalizing to HDAC2 levels for nuclear fractions and to GAPDH levels for cytosolic fractions. Data are mean ± SEM ( n = 5 per group). *** p < 0.001 by t -test.

    Journal: Neurology International

    Article Title: YAP1 Upregulates Cytoskeleton Regulator ARHGEF1 and Tissue Regeneration Factor NEDD9 in a Multiplex Proteomic Study

    doi: 10.3390/neurolint18050096

    Figure Lengend Snippet: YAP1 protein levels are significantly downregulated in the nuclear fractions but not the cytosolic fractions of the AD brains relative to NC brains. Brain lysates were immunoblotted, and protein levels were quantified by normalizing to HDAC2 levels for nuclear fractions and to GAPDH levels for cytosolic fractions. Data are mean ± SEM ( n = 5 per group). *** p < 0.001 by t -test.

    Article Snippet: We cultured the immortalized human neuroblastoma cell line SH-SY5Y (cat # CRL2266, ATCC) in DMEM/F12 medium and infected only once with Lenti ORF particles, human YAP1 (mGFP-tagged) transcript variant 1 (cat # RC225864L4V, Origene, Rockville, MD, USA), and generated stable cells expressing YAP1-GFP with puromycin selection at 5.0 μg per milliliter for two weeks.

    Techniques:

    Antibody-based multiplex microarray screening of 8000 proteins revealed alterations of specific proteins. ( A ) SH-SY5Y cells expressing YAP1-GFP. Scale, 50 µm. ( B ) YAP1-GFP expression supported by immunoblot. ( C ) Examples of glass slide luminescence. ( D ) Heatmap of control and YAP1 biomarkers in 8000 samples. The data were imported to JMP Genomics 10.2 for Hierarchical Cluster analysis.

    Journal: Neurology International

    Article Title: YAP1 Upregulates Cytoskeleton Regulator ARHGEF1 and Tissue Regeneration Factor NEDD9 in a Multiplex Proteomic Study

    doi: 10.3390/neurolint18050096

    Figure Lengend Snippet: Antibody-based multiplex microarray screening of 8000 proteins revealed alterations of specific proteins. ( A ) SH-SY5Y cells expressing YAP1-GFP. Scale, 50 µm. ( B ) YAP1-GFP expression supported by immunoblot. ( C ) Examples of glass slide luminescence. ( D ) Heatmap of control and YAP1 biomarkers in 8000 samples. The data were imported to JMP Genomics 10.2 for Hierarchical Cluster analysis.

    Article Snippet: We cultured the immortalized human neuroblastoma cell line SH-SY5Y (cat # CRL2266, ATCC) in DMEM/F12 medium and infected only once with Lenti ORF particles, human YAP1 (mGFP-tagged) transcript variant 1 (cat # RC225864L4V, Origene, Rockville, MD, USA), and generated stable cells expressing YAP1-GFP with puromycin selection at 5.0 μg per milliliter for two weeks.

    Techniques: Multiplex Assay, Microarray, Expressing, Western Blot, Control

    YAP1 expression increased markers of synaptic endocytosis and ATP-dependent activity. ( A ) Gene ontology (GO) biological process over-representation analysis with 283 differentially expressed biomarkers between YAP1 and control samples. ( B ) GO cellular component over-representation analysis with 283 differentially expressed biomarkers between control and YAP1-expressing SH-SY5Y cells.

    Journal: Neurology International

    Article Title: YAP1 Upregulates Cytoskeleton Regulator ARHGEF1 and Tissue Regeneration Factor NEDD9 in a Multiplex Proteomic Study

    doi: 10.3390/neurolint18050096

    Figure Lengend Snippet: YAP1 expression increased markers of synaptic endocytosis and ATP-dependent activity. ( A ) Gene ontology (GO) biological process over-representation analysis with 283 differentially expressed biomarkers between YAP1 and control samples. ( B ) GO cellular component over-representation analysis with 283 differentially expressed biomarkers between control and YAP1-expressing SH-SY5Y cells.

    Article Snippet: We cultured the immortalized human neuroblastoma cell line SH-SY5Y (cat # CRL2266, ATCC) in DMEM/F12 medium and infected only once with Lenti ORF particles, human YAP1 (mGFP-tagged) transcript variant 1 (cat # RC225864L4V, Origene, Rockville, MD, USA), and generated stable cells expressing YAP1-GFP with puromycin selection at 5.0 μg per milliliter for two weeks.

    Techniques: Expressing, Activity Assay, Control

    ( A ) GO molecular function over-representation analysis with 283 differentially expressed biomarkers between YAP1 and control samples. ( B ) Interaction network of human YAP1 protein as analyzed by STRING.

    Journal: Neurology International

    Article Title: YAP1 Upregulates Cytoskeleton Regulator ARHGEF1 and Tissue Regeneration Factor NEDD9 in a Multiplex Proteomic Study

    doi: 10.3390/neurolint18050096

    Figure Lengend Snippet: ( A ) GO molecular function over-representation analysis with 283 differentially expressed biomarkers between YAP1 and control samples. ( B ) Interaction network of human YAP1 protein as analyzed by STRING.

    Article Snippet: We cultured the immortalized human neuroblastoma cell line SH-SY5Y (cat # CRL2266, ATCC) in DMEM/F12 medium and infected only once with Lenti ORF particles, human YAP1 (mGFP-tagged) transcript variant 1 (cat # RC225864L4V, Origene, Rockville, MD, USA), and generated stable cells expressing YAP1-GFP with puromycin selection at 5.0 μg per milliliter for two weeks.

    Techniques: Control

    The bar graph shows GO analysis results of the increased ( A ) and decreased proteins ( B ) due to YAP1 expression using ShinyGO 0.85.1. Sets of nonredundant, significant GO enrichment terms are displayed to show the foldenrichment and the number of proteins matched to each term. The fold-enrichment values are displayed with the highest on top, and the shade of each bar reflects the number of proteins found in a given GO category.

    Journal: Neurology International

    Article Title: YAP1 Upregulates Cytoskeleton Regulator ARHGEF1 and Tissue Regeneration Factor NEDD9 in a Multiplex Proteomic Study

    doi: 10.3390/neurolint18050096

    Figure Lengend Snippet: The bar graph shows GO analysis results of the increased ( A ) and decreased proteins ( B ) due to YAP1 expression using ShinyGO 0.85.1. Sets of nonredundant, significant GO enrichment terms are displayed to show the foldenrichment and the number of proteins matched to each term. The fold-enrichment values are displayed with the highest on top, and the shade of each bar reflects the number of proteins found in a given GO category.

    Article Snippet: We cultured the immortalized human neuroblastoma cell line SH-SY5Y (cat # CRL2266, ATCC) in DMEM/F12 medium and infected only once with Lenti ORF particles, human YAP1 (mGFP-tagged) transcript variant 1 (cat # RC225864L4V, Origene, Rockville, MD, USA), and generated stable cells expressing YAP1-GFP with puromycin selection at 5.0 μg per milliliter for two weeks.

    Techniques: Expressing

    Evidence of increased levels of ( A ) NEDD9 and ( B ) ARHGEF1 inYAP1-GFP-expressing SH-SY5Y cells when compared to control cells by immunoblotting. ( C ) The LC3-16/LC3-18 ratio was also increased, indicating YAP1 activates autophagy. However, levels of ( D ) p16INK4a, a marker of senescence, were almost completely abolished. Data are mean + SEM, n = 3/group. *, p < 0.05, **, p < 0.01, ***, p < 0.001 by t -test.

    Journal: Neurology International

    Article Title: YAP1 Upregulates Cytoskeleton Regulator ARHGEF1 and Tissue Regeneration Factor NEDD9 in a Multiplex Proteomic Study

    doi: 10.3390/neurolint18050096

    Figure Lengend Snippet: Evidence of increased levels of ( A ) NEDD9 and ( B ) ARHGEF1 inYAP1-GFP-expressing SH-SY5Y cells when compared to control cells by immunoblotting. ( C ) The LC3-16/LC3-18 ratio was also increased, indicating YAP1 activates autophagy. However, levels of ( D ) p16INK4a, a marker of senescence, were almost completely abolished. Data are mean + SEM, n = 3/group. *, p < 0.05, **, p < 0.01, ***, p < 0.001 by t -test.

    Article Snippet: We cultured the immortalized human neuroblastoma cell line SH-SY5Y (cat # CRL2266, ATCC) in DMEM/F12 medium and infected only once with Lenti ORF particles, human YAP1 (mGFP-tagged) transcript variant 1 (cat # RC225864L4V, Origene, Rockville, MD, USA), and generated stable cells expressing YAP1-GFP with puromycin selection at 5.0 μg per milliliter for two weeks.

    Techniques: Expressing, Control, Western Blot, Marker

    Alterations in mammary tumors upon Dnph1 knockout (A) Examples of CD31 staining in mammary tumors from a MMTV-Her2/Dnph1 +/+ and a MMTV-Her2/Dnph1 −/− mouse. Right panels show ImageJ-mediated image modulation to enhance vessel structures, allowing the computer-mediated quantification of blood vessel density. Scale bars, 0.4 mm. (B) Corresponding quantification of vessel density. Tumors were age- and weight-matched; two-tailed, unpaired t test. (C) Tumor vessel density plotted against the weight of the tumor. These tumors were not weight- or age-matched, yet include those shown in panel B. Shown are linear regressions; while the slopes were not significantly different, the Y-intercepts were significantly different ( p = 0.0007). (D) Relative levels of AMP, GMP, and UMP in mammary tumors from MMTV-Her2/Dnph1 +/+ and MMTV-Her2/Dnph1 −/− mice; two-tailed, unpaired t test. (E and F) Immunohistochemical staining for phospho-AMPKα or phospho-YAP1, respectively, on age- and weight-matched tumors as in panel B. Examples of immunohistochemical staining are shown at the bottom. Scale bars, 0.4 mm. Means with standard deviation are shown in panels B and D-F.

    Journal: iScience

    Article Title: Promotion of breast cancer by the DNPH1 enzyme

    doi: 10.1016/j.isci.2026.115227

    Figure Lengend Snippet: Alterations in mammary tumors upon Dnph1 knockout (A) Examples of CD31 staining in mammary tumors from a MMTV-Her2/Dnph1 +/+ and a MMTV-Her2/Dnph1 −/− mouse. Right panels show ImageJ-mediated image modulation to enhance vessel structures, allowing the computer-mediated quantification of blood vessel density. Scale bars, 0.4 mm. (B) Corresponding quantification of vessel density. Tumors were age- and weight-matched; two-tailed, unpaired t test. (C) Tumor vessel density plotted against the weight of the tumor. These tumors were not weight- or age-matched, yet include those shown in panel B. Shown are linear regressions; while the slopes were not significantly different, the Y-intercepts were significantly different ( p = 0.0007). (D) Relative levels of AMP, GMP, and UMP in mammary tumors from MMTV-Her2/Dnph1 +/+ and MMTV-Her2/Dnph1 −/− mice; two-tailed, unpaired t test. (E and F) Immunohistochemical staining for phospho-AMPKα or phospho-YAP1, respectively, on age- and weight-matched tumors as in panel B. Examples of immunohistochemical staining are shown at the bottom. Scale bars, 0.4 mm. Means with standard deviation are shown in panels B and D-F.

    Article Snippet: YAP1 phosphorylated on serine 127 , Cell Signaling , Cat# 4911.

    Techniques: Knock-Out, Staining, Two Tailed Test, Immunohistochemical staining, Standard Deviation

    Model how DNPH1 promotes breast tumor development and progression This model is centered around a DNPH1-YAP1 axis, yet DNPH1 may utilize to-be-identified effectors other than YAP1 to stimulate, e.g., stem cells or angiogenesis.

    Journal: iScience

    Article Title: Promotion of breast cancer by the DNPH1 enzyme

    doi: 10.1016/j.isci.2026.115227

    Figure Lengend Snippet: Model how DNPH1 promotes breast tumor development and progression This model is centered around a DNPH1-YAP1 axis, yet DNPH1 may utilize to-be-identified effectors other than YAP1 to stimulate, e.g., stem cells or angiogenesis.

    Article Snippet: YAP1 phosphorylated on serine 127 , Cell Signaling , Cat# 4911.

    Techniques: