Journal: Cells

Article Title: Conjugated Bile Acids Promote Lymphangiogenesis by Modulation of the Reactive Oxygen Species–p90RSK–Vascular Endothelial Growth Factor Receptor 3 Pathway

doi: 10.3390/cells12040526

Figure Lengend Snippet: Antibodies used in Western blot, immunohistochemistry, and immunofluorescence.

Article Snippet: YAP1 , WB , 1:1000 , Novus , NB110-58358 , AB_922796.

Techniques: Western Blot, Immunohistochemistry, Immunofluorescence

Journal: Developmental cell

Article Title: Yorkie growth promoting activity is limited by Atg1-mediated phosphorylation

doi: 10.1016/j.devcel.2020.01.011

Figure Lengend Snippet: Confocal images of third instar wing discs stained for the ban-LacZ (A-D) reporter of Yorkie transcriptional activity, or the endogenous Diap1 (E-H) and Ex (I-L) proteins. All larvae contained the en-Gal4 driver and only UAS-mCherry (A,E,I), or, in addition, the indicated UAS-RNAi transgenes knocking down Hippo (B,F,J), Atg1 (C,G,K) or Acinus (D,H,L). The mCherry-marked posterior en-Gal4 expression domains are shown in (A’-L’). Images are representative examples from at least three independent crosses with 5 or more examples each. Scale bar = 100 μm. Detailed genotypes are listed in Table S2.

Article Snippet: Proteins used were human ULK1 (Sigma product number: SRP5096 Lot: G1163–1), human TAZ (OriGene Technologies product number: TP304082 Lot: 1160CA), and human Yap1 (OriGene Technologies product number: TP325864 Lot: 10a73e).

Techniques: Staining, Activity Assay, Expressing

Journal: Developmental cell

Article Title: Yorkie growth promoting activity is limited by Atg1-mediated phosphorylation

doi: 10.1016/j.devcel.2020.01.011

Figure Lengend Snippet: (A) Diagram of Atg1 target consensus sequence, compared to potential phosphorylation sites at serines 74, and 97 in Yorkie and corresponding sites in human and mouse YAP and TAZ proteins.

Article Snippet: Proteins used were human ULK1 (Sigma product number: SRP5096 Lot: G1163–1), human TAZ (OriGene Technologies product number: TP304082 Lot: 1160CA), and human Yap1 (OriGene Technologies product number: TP325864 Lot: 10a73e).

Techniques: Sequencing

Journal: Developmental cell

Article Title: Yorkie growth promoting activity is limited by Atg1-mediated phosphorylation

doi: 10.1016/j.devcel.2020.01.011

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Proteins used were human ULK1 (Sigma product number: SRP5096 Lot: G1163–1), human TAZ (OriGene Technologies product number: TP304082 Lot: 1160CA), and human Yap1 (OriGene Technologies product number: TP325864 Lot: 10a73e).

Techniques: Recombinant, SYBR Green Assay, Plasmid Preparation, Protease Inhibitor, Transfection, Chromatography, Electron Microscopy, Software