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Structured Review

Proteintech antitgm2
Antitgm2, supplied by Proteintech, used in various techniques. Bioz Stars score: 92/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/xrcc6/KU70%2CXRCC6+Antibody/pm41831177-78-26-27
Average 92 stars, based on 15 article reviews
antitgm2 - by Bioz Stars, 2026-09
92/100 stars

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Related Articles

Expressing:

Article Title: MGMT activated by Wnt pathway promotes cisplatin tolerance through inducing slow-cycling cells and nonhomologous end joining in colorectal cancer
Article Snippet: .. The tubes were heated and boiled at 100 °C for 10 min. To detect CDK1 (ET1607-51; Huabio, Hangzhou, China), XRCC6 (66607-1-Ig; Proteintech), and HA tag (51064-2-AP; Proteintech) expression, Western blotting was performed. ..

Article Title: MGMT activated by Wnt pathway promotes cisplatin tolerance through inducing slow-cycling cells and nonhomologous end joining in colorectal cancer.
Article Snippet: .. The tubes were heated and boiled at 100 C for 10 min. To detect CDK1 (ET1607-51; Huabio, Hangzhou, China), XRCC6 (66607-1-Ig; Proteintech), and HA tag (51064-2-AP; Proteintech) expression, Western blotting was performed. ..

Western Blot:

Article Title: MGMT activated by Wnt pathway promotes cisplatin tolerance through inducing slow-cycling cells and nonhomologous end joining in colorectal cancer
Article Snippet: .. The tubes were heated and boiled at 100 °C for 10 min. To detect CDK1 (ET1607-51; Huabio, Hangzhou, China), XRCC6 (66607-1-Ig; Proteintech), and HA tag (51064-2-AP; Proteintech) expression, Western blotting was performed. ..

Article Title: MGMT activated by Wnt pathway promotes cisplatin tolerance through inducing slow-cycling cells and nonhomologous end joining in colorectal cancer.
Article Snippet: .. The tubes were heated and boiled at 100 C for 10 min. To detect CDK1 (ET1607-51; Huabio, Hangzhou, China), XRCC6 (66607-1-Ig; Proteintech), and HA tag (51064-2-AP; Proteintech) expression, Western blotting was performed. ..

Staining:


Ubiquitin Proteomics:

Article Title: MGMT activated by Wnt pathway promotes cisplatin tolerance through inducing slow-cycling cells and nonhomologous end joining in colorectal cancer
Article Snippet: Equal amounts of proteins were added to the sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and then transferred to polyvinylidene fluoride (PVDF) blotting membranes (Millipore, Billerica, MA, USA). .. Next, PVDF blotting membranes were blocked with 5% nonfat milk for 2 h at room temperature and blocked with a mixture of proteins targeting MGMT (1:2000; Proteintech), poly (adenosine diphosphate (ADP)-ribose) polymerase 1 (PARP1) (1:2000; Proteintech), cleaved PARP1 (1:1000; Cell Signaling Technology, Inc., Danvers, MA, USA), caspase-3 (1:1000; Cell Signaling Technology, Inc.), cleaved caspase-3 (1:1000; Cell Signaling Technology, Inc.), caspase-7 (1:1000; Cell Signaling Technology, Inc.), cleaved caspase-7 (1:1000; Cell Signaling Technology, Inc.), cyclin D1 (1: 1000; Cell Signaling Technology, Inc.), beta catenin (1:10,000; Proteintech), cyclin dependent kinase inhibitor 1A (P21) (1:1000; Proteintech), cyclin dependent kinase inhibitor 2A (P16) (1:1000; Proteintech), CDK1 (1:1000; Proteintech), XRCC6 (1:10000; Proteintech), HA tag (1:5000; Proteintech), ubiquitin (1:2000; PTM Bio, Hangzhou, China), GAPDH (1:10000; Proteintech), and α-tubulin (1:10000; Proteintech) at 4 °C overnight. .. The membranes were then washed three times with Tris-buffered saline with Tween-20 (TBST) and incubated with the corresponding secondary goat-anti-rabbit IgG (1:10000; Proteintech) or goat-anti-mouse IgG antibody (1:10000; Proteintech) for 2 h at room temperature.

Membrane:

Article Title: Chemoproteomic Profiling of Signaling Metabolite Fructose-1,6-Bisphosphate Interacting Proteins in Living Cells.
Article Snippet: .. The membrane was incubated with primary antibodies GAPDH (Abcam, 1:10000), ALDH2 (Proteintech, 1:3000), ALDH1B1 (Proteintech, 1:3000), SOS1 (Proteintech, 1:3000), XRCC5 (Proteintech, 1:3000), XRCC6 (Proteintech, 1:3000), VIM (Proteintech, 1:3000), MDH2 (Proteintech, 1:3000), ANXA2 (Proteintech, 1:3000), PKM2 (Abcam, 1:3000). ..

Incubation:

Article Title: Chemoproteomic Profiling of Signaling Metabolite Fructose-1,6-Bisphosphate Interacting Proteins in Living Cells.
Article Snippet: .. The membrane was incubated with primary antibodies GAPDH (Abcam, 1:10000), ALDH2 (Proteintech, 1:3000), ALDH1B1 (Proteintech, 1:3000), SOS1 (Proteintech, 1:3000), XRCC5 (Proteintech, 1:3000), XRCC6 (Proteintech, 1:3000), VIM (Proteintech, 1:3000), MDH2 (Proteintech, 1:3000), ANXA2 (Proteintech, 1:3000), PKM2 (Abcam, 1:3000). ..



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