Review




Structured Review

Proteintech xbp1u
Xbp1u, supplied by Proteintech, used in various techniques. Bioz Stars score: 95/100, based on 105 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/xbp/XBP-1U+specific+Antibody/pmc12997330-141-24-25
Average 95 stars, based on 105 article reviews
xbp1u - by Bioz Stars, 2026-09
95/100 stars

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Related Articles

Incubation:

Article Title: Complement 5a receptor 2 attenuates diabetic kidney disease by promoting mitochondria-associated endoplasmic reticulum membrane formation mediated by PSS-MFN2 interaction
Article Snippet: .. The membranes were blocked with 5% skim milk and then incubated with primary antibodies against C5aR2 (sc-515734; Santa Cruz Biotechnology), PSS1 (ab157222; Abcam), PSS2 (ARP49961_P050; Aviva Systems Biology Corporation), MFN2 (12186-1-AP; Proteintech), XBP-1s (143F; BioLegend), p-EIF2α (28740-1-AP; Proteintech), EIF2α (11170-1-AP; Proteintech), CHOP (15204-1-AP; Proteintech), COX IV (11242-1-AP; Proteintech), calnexin (10427-2-AP; Proteintech), ERK1/2 (4695; Cell Signaling Technology), p-ERK1/2 (4370; Cell Signaling Technology), HA (ab9110, Abcam), FLAG (ab213519, Abcam), α-tubulin (HRP-80762; Proteintech), and β-actin (Ac028; ABclonal). .. The membranes were subsequently incubated with HRP-conjugated secondary antibodies (SA00001; Proteintech).

Article Title: Complement 5a receptor 2 attenuates diabetic kidney disease by promoting mitochondria-associated endoplasmic reticulum membrane formation mediated by PSS-MFN2 interaction.
Article Snippet: .. The membranes were blocked with 5% skim milk and then incubated with primary antibodies against C5aR2 (sc-515734; Santa Cruz Biotechnology), PSS1 (ab157222; Abcam), PSS2 (ARP49961_P050; Aviva Systems Biology Corporation), MFN2 (12186-1-AP; Proteintech), XBP-1s (143F; BioLegend), p-EIF2α (28740-1-AP; Proteintech), EIF2α (11170-1-AP; Proteintech), CHOP (15204-1-AP; Proteintech), COX IV (11242-1-AP; Proteintech), calnexin (10427-2-AP; Proteintech), ERK1/2 (4695; Cell Signaling Technology), pERK1/2 (4370; Cell Signaling Technology), HA (ab9110, Abcam), FLAG (ab213519, Abcam), α-tubulin (HRP-80762; Proteintech), and β-actin (Ac028; ABclonal). .. The membranes were subsequently incubated with HRP-conjugated secondary antibodies (SA00001; Proteintech).

Article Title: Tanshinone IIA ameliorates experimental diabetic cardiomyopathy by inhibiting endoplasmic reticulum stress in cardiomyocytes via SIRT1.
Article Snippet: The State Key Laboratory of Functions and Applications of Medicinal Plants, Guizhou Medical University, Guiyang, China The Department of Pharmacology of Materia Medica (The High Efficacy Application of Natural Medicinal Resources Engineering Center of Guizhou Province and The High Educational Key Laboratory of Guizhou Province for Natural Medicinal Pharmacology and Druggability), School of Pharmaceutical Sciences, Guizhou Medical University, Guiyang, China The Key Laboratory of Optimal Utilization of Natural Medicine Resources (The Union Key Laboratory of Guiyang City-Guizhou Medical University), School of Pharmaceutical Sciences, Guizhou Medical University, Guiyang, China The Department of Pharmacology, College of Basic Medical Sciences of Guizhou Medical University, Guiyang, China The Department of Pharmacy, The Second Affiliated Hospital of Guizhou Medical University, Guiyang, China The Key Laboratory of Endemic and Ethnic Diseases of Ministry of Education, Guizhou Medical University, Guiyang, China

Article Title: Mesenchymal Stem Cells Inhibit Epithelial-to-Mesenchymal Transition by Modulating the IRE1 α Branch of the Endoplasmic Reticulum Stress Response
Article Snippet: Total protein was extracted from A549 cells and lung tissues using radioimmunoprecipitation assay (RIPA) buffer (Beyotime, Shanghai, China). .. After the proteins were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, they were transferred to membranes, which were blocked with Protein Free Rapid Blocking Buffer (PS108, EpiZyme) and incubated with primary antibodies against CHOP (A5462, Bimake), BiP (11587-1-AP; Proteintech, Wuhan, China), ATF6 (D262665, Sangon, China), ATF4 (A5514, Bimake), XBP-1s (24868-1-AP, Proteintech), XBP-1u (25997-1-AP, Proteintech), IRE1 α (A00683-1, Boster, Wuhan, China), phospho-IRE1 α (S724, human) (ab124945, Abcam, Cambridge, UK), phospho-IRE1 α (S724, mouse) (530878, ZEN-BIO, Chengdu, China), Vimentin (ET1610-39, HuaBio, Hangzhou, China), and E-cadherin (340341, ZEN-BIO) overnight. .. GAPDH (bs-0755R, Bioss, Beijing, China) was used as the internal reference protein.

Article Title: Co-inhibition of immunoproteasome subunits LMP2 and LMP7 enables prevention of transplant arteriosclerosis.
Article Snippet: . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Co-inhibition of immunoproteasome subunits LMP2 and LMP7 enables prevention of transplant arteriosclerosis

Ubiquitin Proteomics:

Article Title: Dexamethasone-stimulated glucocorticoid receptor signaling positively regulates the endoplasmic reticulum-associated degradation (ERAD) mechanism in hepatocellular carcinoma cells.
Article Snippet: Hepatocellular carcinoma is one of the most common types of primary liver cancer in adults and also it is the third leading cause of cancer-related deaths worldwide.. Although there are various treatment options such as surgery, radiation, targeted drug therapy, immunotherapy and chemotherapy, most hepatocellular carcinomas are highly resistant to systemic treatments.. Today, the molecular pathogenesis of hepatocellular carcinoma remains largely obscure.

Blocking Assay:

Article Title: Tanshinone IIA ameliorates experimental diabetic cardiomyopathy by inhibiting endoplasmic reticulum stress in cardiomyocytes via SIRT1.
Article Snippet: The State Key Laboratory of Functions and Applications of Medicinal Plants, Guizhou Medical University, Guiyang, China The Department of Pharmacology of Materia Medica (The High Efficacy Application of Natural Medicinal Resources Engineering Center of Guizhou Province and The High Educational Key Laboratory of Guizhou Province for Natural Medicinal Pharmacology and Druggability), School of Pharmaceutical Sciences, Guizhou Medical University, Guiyang, China The Key Laboratory of Optimal Utilization of Natural Medicine Resources (The Union Key Laboratory of Guiyang City-Guizhou Medical University), School of Pharmaceutical Sciences, Guizhou Medical University, Guiyang, China The Department of Pharmacology, College of Basic Medical Sciences of Guizhou Medical University, Guiyang, China The Department of Pharmacy, The Second Affiliated Hospital of Guizhou Medical University, Guiyang, China The Key Laboratory of Endemic and Ethnic Diseases of Ministry of Education, Guizhou Medical University, Guiyang, China

Article Title: Mesenchymal Stem Cells Inhibit Epithelial-to-Mesenchymal Transition by Modulating the IRE1 α Branch of the Endoplasmic Reticulum Stress Response
Article Snippet: Total protein was extracted from A549 cells and lung tissues using radioimmunoprecipitation assay (RIPA) buffer (Beyotime, Shanghai, China). .. After the proteins were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, they were transferred to membranes, which were blocked with Protein Free Rapid Blocking Buffer (PS108, EpiZyme) and incubated with primary antibodies against CHOP (A5462, Bimake), BiP (11587-1-AP; Proteintech, Wuhan, China), ATF6 (D262665, Sangon, China), ATF4 (A5514, Bimake), XBP-1s (24868-1-AP, Proteintech), XBP-1u (25997-1-AP, Proteintech), IRE1 α (A00683-1, Boster, Wuhan, China), phospho-IRE1 α (S724, human) (ab124945, Abcam, Cambridge, UK), phospho-IRE1 α (S724, mouse) (530878, ZEN-BIO, Chengdu, China), Vimentin (ET1610-39, HuaBio, Hangzhou, China), and E-cadherin (340341, ZEN-BIO) overnight. .. GAPDH (bs-0755R, Bioss, Beijing, China) was used as the internal reference protein.

Nucleic Acid Electrophoresis:

Article Title: Mesenchymal Stem Cells Inhibit Epithelial-to-Mesenchymal Transition by Modulating the IRE1 α Branch of the Endoplasmic Reticulum Stress Response
Article Snippet: Total protein was extracted from A549 cells and lung tissues using radioimmunoprecipitation assay (RIPA) buffer (Beyotime, Shanghai, China). .. After the proteins were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, they were transferred to membranes, which were blocked with Protein Free Rapid Blocking Buffer (PS108, EpiZyme) and incubated with primary antibodies against CHOP (A5462, Bimake), BiP (11587-1-AP; Proteintech, Wuhan, China), ATF6 (D262665, Sangon, China), ATF4 (A5514, Bimake), XBP-1s (24868-1-AP, Proteintech), XBP-1u (25997-1-AP, Proteintech), IRE1 α (A00683-1, Boster, Wuhan, China), phospho-IRE1 α (S724, human) (ab124945, Abcam, Cambridge, UK), phospho-IRE1 α (S724, mouse) (530878, ZEN-BIO, Chengdu, China), Vimentin (ET1610-39, HuaBio, Hangzhou, China), and E-cadherin (340341, ZEN-BIO) overnight. .. GAPDH (bs-0755R, Bioss, Beijing, China) was used as the internal reference protein.



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Image Search Results


SEC-B induces UPR activation, leading to ER stress. A: Confocal microscopy of ER-Tracker Green-stained HUVECs after 4 and 24 h of treatment with 10 μM SEC-B. The scale bar represents 25 μm. B: TEM images also showed the disorganization and enlargement of ER membranes as indicated by the red arrow (the scale bar represents 0.5 μm). C and D: Western immunoblotting analysis and quantification of XBP-1s and ATF6 expression levels in HUVECs treated with 10 μM SEC-B. Values are the mean ± SD of the values obtained from five independent experiments. ∗∗∗ P < 0.001 versus untreated cells. E: Confocal images of GRP78 immunostaining in HUVECs after 24 h treatment with 10 μM SEC-B. The scale bar represents 20 μm. F: Aggresome formation detected by the ProteoStat® Aggresome Detection Kit after 24 h of 10 μM SEC-B treatment. The scale bar represents 25 μm. Quantitative analysis of positive fluorescent area per cell, in HUVECs treated with 10 μM SEC-B for 24 h. Data are expressed as mean ± SD (n = 3). ∗∗ P < 0.01 versus untreated cells.

Journal: Journal of Lipid Research

Article Title: S-nitrosylation contributes to ER stress and aggresome formation in secosterol-B-mediated endothelial dysfunction

doi: 10.1016/j.jlr.2026.101017

Figure Lengend Snippet: SEC-B induces UPR activation, leading to ER stress. A: Confocal microscopy of ER-Tracker Green-stained HUVECs after 4 and 24 h of treatment with 10 μM SEC-B. The scale bar represents 25 μm. B: TEM images also showed the disorganization and enlargement of ER membranes as indicated by the red arrow (the scale bar represents 0.5 μm). C and D: Western immunoblotting analysis and quantification of XBP-1s and ATF6 expression levels in HUVECs treated with 10 μM SEC-B. Values are the mean ± SD of the values obtained from five independent experiments. ∗∗∗ P < 0.001 versus untreated cells. E: Confocal images of GRP78 immunostaining in HUVECs after 24 h treatment with 10 μM SEC-B. The scale bar represents 20 μm. F: Aggresome formation detected by the ProteoStat® Aggresome Detection Kit after 24 h of 10 μM SEC-B treatment. The scale bar represents 25 μm. Quantitative analysis of positive fluorescent area per cell, in HUVECs treated with 10 μM SEC-B for 24 h. Data are expressed as mean ± SD (n = 3). ∗∗ P < 0.01 versus untreated cells.

Article Snippet: Blots were probed with the following antibodies: anti-spliced X-box binding protein 1 (XBP-1s) (E9V3E, #40435), ATF6 (D4Z8V, #65880), BiP/GRP78 (C50B12, #3177), and protein disulfide isomerase (PDI) (#2446) from Cell Signaling Technology; anti-endothelial NOS (eNOS) (A1548) from ABclonal; and anti-NOS2 (N-20, sc-651) from Santa Cruz Biotechnology.

Techniques: Activation Assay, Confocal Microscopy, Staining, Western Blot, Expressing, Immunostaining