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Proteintech wuhan
Wuhan, supplied by Proteintech, used in various techniques. Bioz Stars score: 98/100, based on 10433 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/wuhan/GAPDH+Monoclonal+antibody/pmc13011214-154-73-72
Average 98 stars, based on 10433 article reviews
wuhan - by Bioz Stars, 2026-09
98/100 stars

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Related Articles

Immunohistochemistry:

Article Title: PKA and PKC signaling pathways mediate vitamin D₃-regulated intestinal phosphorus absorption in broiler chickens
Article Snippet: PVDF membranes were incubated with primary antibodies against NaPi-IIb (1:1000, A9460; ABclonal, Wuhan, China), phosphorylated PKA (p-PKA; 1:1000, 5661S), phosphorylated PI3K (p-PI3K; 1:1000, 4228), phosphorylated ERK (p-ERK; 1:1000, 9101S), phosphorylated p38MAPK (p-p38MAPK; 1:1000, 9216S), total PKA (t-PKA; 1:1000, 5842S) (Cell Signaling Technology, Boston, USA), total PI3K (t-PI3K; 1:10000, 60225-1-Ig), total ERK (t-ERK; 1:8000, 11257-1-AP-50), total p38MAPK(t-p38MAPK; 1:1000, 14064-1-AP), total PKC (t-PKC; 1:1000, 21991-1-AP), phosphorylated PKC (p-PKC; 1:5000, 29123-1-AP), and GAPDH (1:10000, 60004-1-Ig) (Proteintech, Wuhan, China).

Article Title: miR-3614-5p prevents endothelial differentiation and angiogenesis in human exfoliated deciduous teeth by targeting lysophosphatidic acid receptor 2.
Article Snippet: The contribution of microRNAs (miRNAs) to the endothelial differentiation of stem cells from human exfoliated deciduous teeth (SHED) remains largely unclear.. This study sought to uncover novel miRNAs implicated in this process.. SHED were obtained from deciduous teeth and verified via flow cytometric profiling of CD34, CD90, and CD105.

Article Title: The PRKN/METTL3/CLDN2 axis promotes colorectal cancer development through epigenetic mechanisms.
Article Snippet: For IHC staining as described [21], slides (4 μm) from clinical CRC tumors and mouse xenografts, which were fixed and embedded in paraffin, were probed with anti-METTL3 (rabbit polyclonal (pAb), 1:1,500, Cat. No. 15073-1-AP, Proteintech, Wuhan, China), anti-CLDN2 (rabbit monoclonal (mAb), 1:600, Cat. No. 18932, Cell Signaling Technology, Danvers, MA, USA), anti-Ki67 (mouse mAb, 1:5,000, Cat. No. 9449, Cell Signaling Technology), anti-PCNA (rabbit pAb, 1:3,500, Cat. No. 10205-2-AP, Proteintech), and anti-MMP9 (mouse mAb, 1:900, Cat. No. ab58803, Abcam, Cambridge, UK) antibodies.

Article Title: Resveratrol Mitigates Age-Associated Cognitive Decline via Inhibition of cGAS-STING-Mediated Microglial Senescence.
Article Snippet: The sections were subjected to antigen retrieval with sodium citrate (10 mM, pH 6.0) at 95 ◦C for 20 min, then blocked in 5% normal donkey serum, 5% bovine serum albumin (BSA, Millipore Sigma, #A1933, Burlington, MA, USA) and 0.1% TritonX-100/PBS for 1 h, followed by incubation with primary antibodies (pTBK1, 1:100, #5483S, Cell Signaling Technology, Danvers, MA, USA; IBA1, 1:1000, #019-19741, Wako, Tokyo, Japan; P2ry12, 1:100, #848002, Biolegend, CA, USA; TUB33, 1:500, #66375- 1-lg, Proteintech, Wuhan, China; CD68, 1:100, #MCA1957, Bio-Rad, Hercules, CA, USA; β-gal, 1:200, #15518-1-AP, Proteintech, Wuhan, China), diluted with 1% BSA and 0.02% tween in PBS overnight.

Article Title: Mechanism of platelet-rich plasma inhibiting inflammatory response, oxidative stress, and senescence in synovial fibroblasts of patients with knee osteoarthritis via UBE2C based on transcriptome sequencing analysis.
Article Snippet: Extended author information available on the last page of the article Abstract Background The incidence of knee osteoarthritis (KOA) continues to rise with the aging of the population, which seriously reduces the quality of life of patients.. Platelet-rich plasma (PRP), a biological agent rich in growth factors and cytokines, has shown promising potential in the treatment of KOA in recent years.. However, the specific molecular mechanism of its regulation of the pathological process is not clear.

Article Title: A precise theranostic nanoplatform amplifies anti-tumor efficacy via copper ionophores and sonodynamic therapy
Article Snippet: Cells were then incubated overnight at 4 °C with primary rabbit polyclonal antibodies against DLAT (13426-1-AP, 1:200), FDX1 (12592-1-AP, 1:250), CRT (10292-1-AP, 1:200), and HMGB1 (10829-1-AP, 1:200) (Proteintech, Wuhan, China).

Article Title: Nicotinamide N-Methyltransferase Inhibition Mitigates Cerulein-Induced Pancreatic Fibrosis via Galectin-3-Mediated Regulation of Stellate Cell Activation and Macrophage M2 Polarization in Mice.
Article Snippet: Table 1 Immunofluorescence antibody information Antibody name Dilution rate Item number Manufacturer desmin 1: 200 67793-1-Ig Proteintech, Wuhan, Hubei, China α-SMA 1: 200 67735-1-Ig Proteintech, Wuhan, Hubei, China NNMT 1: 100 15123-1-AP Proteintech, Wuhan, Hubei, China F4/80 1: 500 28463-1-AP Proteintech, Wuhan, Hubei, China CD206 1: 50 sc-70586 Santa cruz, Dallas, Texas, USA Collagen I 1: 200 14695-1-AP Proteintech, Wuhan, Hubei, China Goat Anti-Rabbit 1: 200 ab6717 Abcam, Cambridge, UK

Staining:

Article Title: PKA and PKC signaling pathways mediate vitamin D₃-regulated intestinal phosphorus absorption in broiler chickens
Article Snippet: PVDF membranes were incubated with primary antibodies against NaPi-IIb (1:1000, A9460; ABclonal, Wuhan, China), phosphorylated PKA (p-PKA; 1:1000, 5661S), phosphorylated PI3K (p-PI3K; 1:1000, 4228), phosphorylated ERK (p-ERK; 1:1000, 9101S), phosphorylated p38MAPK (p-p38MAPK; 1:1000, 9216S), total PKA (t-PKA; 1:1000, 5842S) (Cell Signaling Technology, Boston, USA), total PI3K (t-PI3K; 1:10000, 60225-1-Ig), total ERK (t-ERK; 1:8000, 11257-1-AP-50), total p38MAPK(t-p38MAPK; 1:1000, 14064-1-AP), total PKC (t-PKC; 1:1000, 21991-1-AP), phosphorylated PKC (p-PKC; 1:5000, 29123-1-AP), and GAPDH (1:10000, 60004-1-Ig) (Proteintech, Wuhan, China).

Article Title: miR-3614-5p prevents endothelial differentiation and angiogenesis in human exfoliated deciduous teeth by targeting lysophosphatidic acid receptor 2.
Article Snippet: The contribution of microRNAs (miRNAs) to the endothelial differentiation of stem cells from human exfoliated deciduous teeth (SHED) remains largely unclear.. This study sought to uncover novel miRNAs implicated in this process.. SHED were obtained from deciduous teeth and verified via flow cytometric profiling of CD34, CD90, and CD105.

Article Title: The PRKN/METTL3/CLDN2 axis promotes colorectal cancer development through epigenetic mechanisms.
Article Snippet: For IHC staining as described [21], slides (4 μm) from clinical CRC tumors and mouse xenografts, which were fixed and embedded in paraffin, were probed with anti-METTL3 (rabbit polyclonal (pAb), 1:1,500, Cat. No. 15073-1-AP, Proteintech, Wuhan, China), anti-CLDN2 (rabbit monoclonal (mAb), 1:600, Cat. No. 18932, Cell Signaling Technology, Danvers, MA, USA), anti-Ki67 (mouse mAb, 1:5,000, Cat. No. 9449, Cell Signaling Technology), anti-PCNA (rabbit pAb, 1:3,500, Cat. No. 10205-2-AP, Proteintech), and anti-MMP9 (mouse mAb, 1:900, Cat. No. ab58803, Abcam, Cambridge, UK) antibodies.

Article Title: Resveratrol Mitigates Age-Associated Cognitive Decline via Inhibition of cGAS-STING-Mediated Microglial Senescence.
Article Snippet: The sections were subjected to antigen retrieval with sodium citrate (10 mM, pH 6.0) at 95 ◦C for 20 min, then blocked in 5% normal donkey serum, 5% bovine serum albumin (BSA, Millipore Sigma, #A1933, Burlington, MA, USA) and 0.1% TritonX-100/PBS for 1 h, followed by incubation with primary antibodies (pTBK1, 1:100, #5483S, Cell Signaling Technology, Danvers, MA, USA; IBA1, 1:1000, #019-19741, Wako, Tokyo, Japan; P2ry12, 1:100, #848002, Biolegend, CA, USA; TUB33, 1:500, #66375- 1-lg, Proteintech, Wuhan, China; CD68, 1:100, #MCA1957, Bio-Rad, Hercules, CA, USA; β-gal, 1:200, #15518-1-AP, Proteintech, Wuhan, China), diluted with 1% BSA and 0.02% tween in PBS overnight.

Article Title: Mechanism of platelet-rich plasma inhibiting inflammatory response, oxidative stress, and senescence in synovial fibroblasts of patients with knee osteoarthritis via UBE2C based on transcriptome sequencing analysis.
Article Snippet: Extended author information available on the last page of the article Abstract Background The incidence of knee osteoarthritis (KOA) continues to rise with the aging of the population, which seriously reduces the quality of life of patients.. Platelet-rich plasma (PRP), a biological agent rich in growth factors and cytokines, has shown promising potential in the treatment of KOA in recent years.. However, the specific molecular mechanism of its regulation of the pathological process is not clear.

Article Title: A precise theranostic nanoplatform amplifies anti-tumor efficacy via copper ionophores and sonodynamic therapy
Article Snippet: Cells were then incubated overnight at 4 °C with primary rabbit polyclonal antibodies against DLAT (13426-1-AP, 1:200), FDX1 (12592-1-AP, 1:250), CRT (10292-1-AP, 1:200), and HMGB1 (10829-1-AP, 1:200) (Proteintech, Wuhan, China).

Article Title: Nicotinamide N-Methyltransferase Inhibition Mitigates Cerulein-Induced Pancreatic Fibrosis via Galectin-3-Mediated Regulation of Stellate Cell Activation and Macrophage M2 Polarization in Mice.
Article Snippet: Table 1 Immunofluorescence antibody information Antibody name Dilution rate Item number Manufacturer desmin 1: 200 67793-1-Ig Proteintech, Wuhan, Hubei, China α-SMA 1: 200 67735-1-Ig Proteintech, Wuhan, Hubei, China NNMT 1: 100 15123-1-AP Proteintech, Wuhan, Hubei, China F4/80 1: 500 28463-1-AP Proteintech, Wuhan, Hubei, China CD206 1: 50 sc-70586 Santa cruz, Dallas, Texas, USA Collagen I 1: 200 14695-1-AP Proteintech, Wuhan, Hubei, China Goat Anti-Rabbit 1: 200 ab6717 Abcam, Cambridge, UK

Sequencing:

Article Title: PKA and PKC signaling pathways mediate vitamin D₃-regulated intestinal phosphorus absorption in broiler chickens
Article Snippet: PVDF membranes were incubated with primary antibodies against NaPi-IIb (1:1000, A9460; ABclonal, Wuhan, China), phosphorylated PKA (p-PKA; 1:1000, 5661S), phosphorylated PI3K (p-PI3K; 1:1000, 4228), phosphorylated ERK (p-ERK; 1:1000, 9101S), phosphorylated p38MAPK (p-p38MAPK; 1:1000, 9216S), total PKA (t-PKA; 1:1000, 5842S) (Cell Signaling Technology, Boston, USA), total PI3K (t-PI3K; 1:10000, 60225-1-Ig), total ERK (t-ERK; 1:8000, 11257-1-AP-50), total p38MAPK(t-p38MAPK; 1:1000, 14064-1-AP), total PKC (t-PKC; 1:1000, 21991-1-AP), phosphorylated PKC (p-PKC; 1:5000, 29123-1-AP), and GAPDH (1:10000, 60004-1-Ig) (Proteintech, Wuhan, China).

Article Title: miR-3614-5p prevents endothelial differentiation and angiogenesis in human exfoliated deciduous teeth by targeting lysophosphatidic acid receptor 2.
Article Snippet: The contribution of microRNAs (miRNAs) to the endothelial differentiation of stem cells from human exfoliated deciduous teeth (SHED) remains largely unclear.. This study sought to uncover novel miRNAs implicated in this process.. SHED were obtained from deciduous teeth and verified via flow cytometric profiling of CD34, CD90, and CD105.

Article Title: The PRKN/METTL3/CLDN2 axis promotes colorectal cancer development through epigenetic mechanisms.
Article Snippet: For IHC staining as described [21], slides (4 μm) from clinical CRC tumors and mouse xenografts, which were fixed and embedded in paraffin, were probed with anti-METTL3 (rabbit polyclonal (pAb), 1:1,500, Cat. No. 15073-1-AP, Proteintech, Wuhan, China), anti-CLDN2 (rabbit monoclonal (mAb), 1:600, Cat. No. 18932, Cell Signaling Technology, Danvers, MA, USA), anti-Ki67 (mouse mAb, 1:5,000, Cat. No. 9449, Cell Signaling Technology), anti-PCNA (rabbit pAb, 1:3,500, Cat. No. 10205-2-AP, Proteintech), and anti-MMP9 (mouse mAb, 1:900, Cat. No. ab58803, Abcam, Cambridge, UK) antibodies.

Article Title: Resveratrol Mitigates Age-Associated Cognitive Decline via Inhibition of cGAS-STING-Mediated Microglial Senescence.
Article Snippet: The sections were subjected to antigen retrieval with sodium citrate (10 mM, pH 6.0) at 95 ◦C for 20 min, then blocked in 5% normal donkey serum, 5% bovine serum albumin (BSA, Millipore Sigma, #A1933, Burlington, MA, USA) and 0.1% TritonX-100/PBS for 1 h, followed by incubation with primary antibodies (pTBK1, 1:100, #5483S, Cell Signaling Technology, Danvers, MA, USA; IBA1, 1:1000, #019-19741, Wako, Tokyo, Japan; P2ry12, 1:100, #848002, Biolegend, CA, USA; TUB33, 1:500, #66375- 1-lg, Proteintech, Wuhan, China; CD68, 1:100, #MCA1957, Bio-Rad, Hercules, CA, USA; β-gal, 1:200, #15518-1-AP, Proteintech, Wuhan, China), diluted with 1% BSA and 0.02% tween in PBS overnight.

Article Title: Mechanism of platelet-rich plasma inhibiting inflammatory response, oxidative stress, and senescence in synovial fibroblasts of patients with knee osteoarthritis via UBE2C based on transcriptome sequencing analysis.
Article Snippet: Extended author information available on the last page of the article Abstract Background The incidence of knee osteoarthritis (KOA) continues to rise with the aging of the population, which seriously reduces the quality of life of patients.. Platelet-rich plasma (PRP), a biological agent rich in growth factors and cytokines, has shown promising potential in the treatment of KOA in recent years.. However, the specific molecular mechanism of its regulation of the pathological process is not clear.

Article Title: A precise theranostic nanoplatform amplifies anti-tumor efficacy via copper ionophores and sonodynamic therapy
Article Snippet: Cells were then incubated overnight at 4 °C with primary rabbit polyclonal antibodies against DLAT (13426-1-AP, 1:200), FDX1 (12592-1-AP, 1:250), CRT (10292-1-AP, 1:200), and HMGB1 (10829-1-AP, 1:200) (Proteintech, Wuhan, China).

Article Title: Nicotinamide N-Methyltransferase Inhibition Mitigates Cerulein-Induced Pancreatic Fibrosis via Galectin-3-Mediated Regulation of Stellate Cell Activation and Macrophage M2 Polarization in Mice.
Article Snippet: Table 1 Immunofluorescence antibody information Antibody name Dilution rate Item number Manufacturer desmin 1: 200 67793-1-Ig Proteintech, Wuhan, Hubei, China α-SMA 1: 200 67735-1-Ig Proteintech, Wuhan, Hubei, China NNMT 1: 100 15123-1-AP Proteintech, Wuhan, Hubei, China F4/80 1: 500 28463-1-AP Proteintech, Wuhan, Hubei, China CD206 1: 50 sc-70586 Santa cruz, Dallas, Texas, USA Collagen I 1: 200 14695-1-AP Proteintech, Wuhan, Hubei, China Goat Anti-Rabbit 1: 200 ab6717 Abcam, Cambridge, UK

Countercurrent Chromatography:

Article Title: PKA and PKC signaling pathways mediate vitamin D₃-regulated intestinal phosphorus absorption in broiler chickens
Article Snippet: PVDF membranes were incubated with primary antibodies against NaPi-IIb (1:1000, A9460; ABclonal, Wuhan, China), phosphorylated PKA (p-PKA; 1:1000, 5661S), phosphorylated PI3K (p-PI3K; 1:1000, 4228), phosphorylated ERK (p-ERK; 1:1000, 9101S), phosphorylated p38MAPK (p-p38MAPK; 1:1000, 9216S), total PKA (t-PKA; 1:1000, 5842S) (Cell Signaling Technology, Boston, USA), total PI3K (t-PI3K; 1:10000, 60225-1-Ig), total ERK (t-ERK; 1:8000, 11257-1-AP-50), total p38MAPK(t-p38MAPK; 1:1000, 14064-1-AP), total PKC (t-PKC; 1:1000, 21991-1-AP), phosphorylated PKC (p-PKC; 1:5000, 29123-1-AP), and GAPDH (1:10000, 60004-1-Ig) (Proteintech, Wuhan, China).

Article Title: miR-3614-5p prevents endothelial differentiation and angiogenesis in human exfoliated deciduous teeth by targeting lysophosphatidic acid receptor 2.
Article Snippet: The contribution of microRNAs (miRNAs) to the endothelial differentiation of stem cells from human exfoliated deciduous teeth (SHED) remains largely unclear.. This study sought to uncover novel miRNAs implicated in this process.. SHED were obtained from deciduous teeth and verified via flow cytometric profiling of CD34, CD90, and CD105.

Article Title: The PRKN/METTL3/CLDN2 axis promotes colorectal cancer development through epigenetic mechanisms.
Article Snippet: For IHC staining as described [21], slides (4 μm) from clinical CRC tumors and mouse xenografts, which were fixed and embedded in paraffin, were probed with anti-METTL3 (rabbit polyclonal (pAb), 1:1,500, Cat. No. 15073-1-AP, Proteintech, Wuhan, China), anti-CLDN2 (rabbit monoclonal (mAb), 1:600, Cat. No. 18932, Cell Signaling Technology, Danvers, MA, USA), anti-Ki67 (mouse mAb, 1:5,000, Cat. No. 9449, Cell Signaling Technology), anti-PCNA (rabbit pAb, 1:3,500, Cat. No. 10205-2-AP, Proteintech), and anti-MMP9 (mouse mAb, 1:900, Cat. No. ab58803, Abcam, Cambridge, UK) antibodies.

Article Title: Resveratrol Mitigates Age-Associated Cognitive Decline via Inhibition of cGAS-STING-Mediated Microglial Senescence.
Article Snippet: The sections were subjected to antigen retrieval with sodium citrate (10 mM, pH 6.0) at 95 ◦C for 20 min, then blocked in 5% normal donkey serum, 5% bovine serum albumin (BSA, Millipore Sigma, #A1933, Burlington, MA, USA) and 0.1% TritonX-100/PBS for 1 h, followed by incubation with primary antibodies (pTBK1, 1:100, #5483S, Cell Signaling Technology, Danvers, MA, USA; IBA1, 1:1000, #019-19741, Wako, Tokyo, Japan; P2ry12, 1:100, #848002, Biolegend, CA, USA; TUB33, 1:500, #66375- 1-lg, Proteintech, Wuhan, China; CD68, 1:100, #MCA1957, Bio-Rad, Hercules, CA, USA; β-gal, 1:200, #15518-1-AP, Proteintech, Wuhan, China), diluted with 1% BSA and 0.02% tween in PBS overnight.

Article Title: Mechanism of platelet-rich plasma inhibiting inflammatory response, oxidative stress, and senescence in synovial fibroblasts of patients with knee osteoarthritis via UBE2C based on transcriptome sequencing analysis.
Article Snippet: Extended author information available on the last page of the article Abstract Background The incidence of knee osteoarthritis (KOA) continues to rise with the aging of the population, which seriously reduces the quality of life of patients.. Platelet-rich plasma (PRP), a biological agent rich in growth factors and cytokines, has shown promising potential in the treatment of KOA in recent years.. However, the specific molecular mechanism of its regulation of the pathological process is not clear.

Article Title: A precise theranostic nanoplatform amplifies anti-tumor efficacy via copper ionophores and sonodynamic therapy
Article Snippet: Cells were then incubated overnight at 4 °C with primary rabbit polyclonal antibodies against DLAT (13426-1-AP, 1:200), FDX1 (12592-1-AP, 1:250), CRT (10292-1-AP, 1:200), and HMGB1 (10829-1-AP, 1:200) (Proteintech, Wuhan, China).

Article Title: Nicotinamide N-Methyltransferase Inhibition Mitigates Cerulein-Induced Pancreatic Fibrosis via Galectin-3-Mediated Regulation of Stellate Cell Activation and Macrophage M2 Polarization in Mice.
Article Snippet: Table 1 Immunofluorescence antibody information Antibody name Dilution rate Item number Manufacturer desmin 1: 200 67793-1-Ig Proteintech, Wuhan, Hubei, China α-SMA 1: 200 67735-1-Ig Proteintech, Wuhan, Hubei, China NNMT 1: 100 15123-1-AP Proteintech, Wuhan, Hubei, China F4/80 1: 500 28463-1-AP Proteintech, Wuhan, Hubei, China CD206 1: 50 sc-70586 Santa cruz, Dallas, Texas, USA Collagen I 1: 200 14695-1-AP Proteintech, Wuhan, Hubei, China Goat Anti-Rabbit 1: 200 ab6717 Abcam, Cambridge, UK



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