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a301 152a  (Bethyl)


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    Bethyl a301 152a
    A301 152a, supplied by Bethyl, used in various techniques. Bioz Stars score: 93/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/a301 152a/product/Bethyl
    Average 93 stars, based on 12 article reviews
    a301 152a - by Bioz Stars, 2026-05
    93/100 stars

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    Image Search Results


    Recognition of the AAUAAU hexamer by human mPSF. ( A ) Overlay of the structure of the AAUAAU quaternary complex (CPSF160: deep salmon, WDR33: marine, CPSF30: green, RNA: orange) with that of the AAUAAA complex (gray). The superposition is based on WDR33. ( B ) EM density for the AAUAAU hexamer. ( C ) Overlay of the overall binding modes of AAUAAU (in color) and AAUAAA (gray). ( D ) Overlay of the binding mode of U3-U6 in the AAUAAU complex (in color) with that of U3-A6 in the AAUAAA complex (gray).

    Journal: Nucleic Acids Research

    Article Title: Molecular basis for the recognition of low-frequency polyadenylation signals by mPSF

    doi: 10.1093/nar/gkaf890

    Figure Lengend Snippet: Recognition of the AAUAAU hexamer by human mPSF. ( A ) Overlay of the structure of the AAUAAU quaternary complex (CPSF160: deep salmon, WDR33: marine, CPSF30: green, RNA: orange) with that of the AAUAAA complex (gray). The superposition is based on WDR33. ( B ) EM density for the AAUAAU hexamer. ( C ) Overlay of the overall binding modes of AAUAAU (in color) and AAUAAA (gray). ( D ) Overlay of the binding mode of U3-U6 in the AAUAAU complex (in color) with that of U3-A6 in the AAUAAA complex (gray).

    Article Snippet: WDR33 (residues 1–572) was inserted into pFastBac (438-C) vector (Addgene, Plasmid #55220), which carried an amino-terminal His 6 -MBP tag followed by a TEV protease cleavage site.

    Techniques: Binding Assay

    Recognition of the AGUAAA hexamer by human mPSF. ( A ) Overlay of the structure of the AGUAAA quaternary complex (CPSF160: deep salmon, WDR33: marine, CPSF30: green, RNA: magenta) with that of the AAUAAA complex (gray). The superposition is based on WDR33. ( B ) EM density for the AGUAAA hexamer. ( C ) Overlay of the overall binding modes of AGUAAA (in color) and AAUAAA (gray). ( D ) Overlay of the binding mode of A1-G2 in the AGUAAA complex (in color) with that of A1-A2 in the AAUAAA complex (gray).

    Journal: Nucleic Acids Research

    Article Title: Molecular basis for the recognition of low-frequency polyadenylation signals by mPSF

    doi: 10.1093/nar/gkaf890

    Figure Lengend Snippet: Recognition of the AGUAAA hexamer by human mPSF. ( A ) Overlay of the structure of the AGUAAA quaternary complex (CPSF160: deep salmon, WDR33: marine, CPSF30: green, RNA: magenta) with that of the AAUAAA complex (gray). The superposition is based on WDR33. ( B ) EM density for the AGUAAA hexamer. ( C ) Overlay of the overall binding modes of AGUAAA (in color) and AAUAAA (gray). ( D ) Overlay of the binding mode of A1-G2 in the AGUAAA complex (in color) with that of A1-A2 in the AAUAAA complex (gray).

    Article Snippet: WDR33 (residues 1–572) was inserted into pFastBac (438-C) vector (Addgene, Plasmid #55220), which carried an amino-terminal His 6 -MBP tag followed by a TEV protease cleavage site.

    Techniques: Binding Assay

    Validation (knockdown) results for selected genes.

    Journal: Scientific Reports

    Article Title: In vitro evolution and whole genome analysis to study chemotherapy drug resistance in haploid human cells

    doi: 10.1038/s41598-024-63943-7

    Figure Lengend Snippet: Validation (knockdown) results for selected genes.

    Article Snippet: shRNAs pools targeting TOP2A (Cat# sc-36695-V), DCK (Cat# sc-60509-V), SLCO3A1 (Cat# sc-62713-V), SLC13A4 (Cat# sc-89760-V), KLF-1 (Cat# sc-37831-V), WWOX (Cat# sc-44193-V), WDR33 (Cat# sc-94735-V) and the non-coding control (Cat# sc-108080) were obtained in pLKO.1-Puro from Santa Cruz Biotechnology.

    Techniques: Biomarker Discovery, Knockdown, Tandem Mass Spectroscopy