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reference strain vr2332  (ATCC)


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    ATCC reference strain vr2332
    Experimental layout. Lungs were collected from influenza- and PRRSV-negative 8–12-week-old pigs. Mononuclear phagocytes (MNPs) from bronchoalveolar lavage (BAL) and parenchyma (PAR) were enriched using OptiPrep gradient. MNPs were infected with North Carolina (NC) PRRSV-2 strains NC134 and NC174 (MOI = 1) and control media for 12 h. Infected and control porcine alveolar macrophages (PAMs), pulmonary intravascular macrophages (PIMs), monocyte-derived dendritic cells (moDCs), and classical DCs (cDCs) were sorted, and RNA-seq was performed on each cell subset. Additional PAM and PIM sorted cells were used for qPCR and NanoString validations. For other assays, enriched MNPs were plated to allow macrophage adhesion for 2 h. PAMs and PIMs were subsequently infected with different NC PRRSV-2 strains (NC134, NC174, and NC144) or <t>VR2332</t> prototype strain; uninfected cells were used as negative control. Infected macrophages were used in different assays: mitochondrial function assay (Seahorse Agilent technology) and ROS and NO production assays. Created in BioRender. Crisci, E (2025). https://BioRender.com/65x4hji . PRRSV, porcine reproductive and respiratory syndrome virus; MOI, multiplicity of infection; ROS, reactive oxygen species; NO, nitric oxide.
    Reference Strain Vr2332, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 57 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 94 stars, based on 57 article reviews
    reference strain vr2332 - by Bioz Stars, 2026-03
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    1) Product Images from "Mitochondrial dysfunction in PRRSV-2-infected macrophages"

    Article Title: Mitochondrial dysfunction in PRRSV-2-infected macrophages

    Journal: Frontiers in Immunology

    doi: 10.3389/fimmu.2025.1670488

    Experimental layout. Lungs were collected from influenza- and PRRSV-negative 8–12-week-old pigs. Mononuclear phagocytes (MNPs) from bronchoalveolar lavage (BAL) and parenchyma (PAR) were enriched using OptiPrep gradient. MNPs were infected with North Carolina (NC) PRRSV-2 strains NC134 and NC174 (MOI = 1) and control media for 12 h. Infected and control porcine alveolar macrophages (PAMs), pulmonary intravascular macrophages (PIMs), monocyte-derived dendritic cells (moDCs), and classical DCs (cDCs) were sorted, and RNA-seq was performed on each cell subset. Additional PAM and PIM sorted cells were used for qPCR and NanoString validations. For other assays, enriched MNPs were plated to allow macrophage adhesion for 2 h. PAMs and PIMs were subsequently infected with different NC PRRSV-2 strains (NC134, NC174, and NC144) or VR2332 prototype strain; uninfected cells were used as negative control. Infected macrophages were used in different assays: mitochondrial function assay (Seahorse Agilent technology) and ROS and NO production assays. Created in BioRender. Crisci, E (2025). https://BioRender.com/65x4hji . PRRSV, porcine reproductive and respiratory syndrome virus; MOI, multiplicity of infection; ROS, reactive oxygen species; NO, nitric oxide.
    Figure Legend Snippet: Experimental layout. Lungs were collected from influenza- and PRRSV-negative 8–12-week-old pigs. Mononuclear phagocytes (MNPs) from bronchoalveolar lavage (BAL) and parenchyma (PAR) were enriched using OptiPrep gradient. MNPs were infected with North Carolina (NC) PRRSV-2 strains NC134 and NC174 (MOI = 1) and control media for 12 h. Infected and control porcine alveolar macrophages (PAMs), pulmonary intravascular macrophages (PIMs), monocyte-derived dendritic cells (moDCs), and classical DCs (cDCs) were sorted, and RNA-seq was performed on each cell subset. Additional PAM and PIM sorted cells were used for qPCR and NanoString validations. For other assays, enriched MNPs were plated to allow macrophage adhesion for 2 h. PAMs and PIMs were subsequently infected with different NC PRRSV-2 strains (NC134, NC174, and NC144) or VR2332 prototype strain; uninfected cells were used as negative control. Infected macrophages were used in different assays: mitochondrial function assay (Seahorse Agilent technology) and ROS and NO production assays. Created in BioRender. Crisci, E (2025). https://BioRender.com/65x4hji . PRRSV, porcine reproductive and respiratory syndrome virus; MOI, multiplicity of infection; ROS, reactive oxygen species; NO, nitric oxide.

    Techniques Used: Infection, Control, Derivative Assay, RNA Sequencing, Negative Control, Functional Assay, Virus



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    Experimental layout. Lungs were collected from influenza- and PRRSV-negative 8–12-week-old pigs. Mononuclear phagocytes (MNPs) from bronchoalveolar lavage (BAL) and parenchyma (PAR) were enriched using OptiPrep gradient. MNPs were infected with North Carolina (NC) PRRSV-2 strains NC134 and NC174 (MOI = 1) and control media for 12 h. Infected and control porcine alveolar macrophages (PAMs), pulmonary intravascular macrophages (PIMs), monocyte-derived dendritic cells (moDCs), and classical DCs (cDCs) were sorted, and RNA-seq was performed on each cell subset. Additional PAM and PIM sorted cells were used for qPCR and NanoString validations. For other assays, enriched MNPs were plated to allow macrophage adhesion for 2 h. PAMs and PIMs were subsequently infected with different NC PRRSV-2 strains (NC134, NC174, and NC144) or <t>VR2332</t> prototype strain; uninfected cells were used as negative control. Infected macrophages were used in different assays: mitochondrial function assay (Seahorse Agilent technology) and ROS and NO production assays. Created in BioRender. Crisci, E (2025). https://BioRender.com/65x4hji . PRRSV, porcine reproductive and respiratory syndrome virus; MOI, multiplicity of infection; ROS, reactive oxygen species; NO, nitric oxide.
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    Image Search Results


    Experimental layout. Lungs were collected from influenza- and PRRSV-negative 8–12-week-old pigs. Mononuclear phagocytes (MNPs) from bronchoalveolar lavage (BAL) and parenchyma (PAR) were enriched using OptiPrep gradient. MNPs were infected with North Carolina (NC) PRRSV-2 strains NC134 and NC174 (MOI = 1) and control media for 12 h. Infected and control porcine alveolar macrophages (PAMs), pulmonary intravascular macrophages (PIMs), monocyte-derived dendritic cells (moDCs), and classical DCs (cDCs) were sorted, and RNA-seq was performed on each cell subset. Additional PAM and PIM sorted cells were used for qPCR and NanoString validations. For other assays, enriched MNPs were plated to allow macrophage adhesion for 2 h. PAMs and PIMs were subsequently infected with different NC PRRSV-2 strains (NC134, NC174, and NC144) or VR2332 prototype strain; uninfected cells were used as negative control. Infected macrophages were used in different assays: mitochondrial function assay (Seahorse Agilent technology) and ROS and NO production assays. Created in BioRender. Crisci, E (2025). https://BioRender.com/65x4hji . PRRSV, porcine reproductive and respiratory syndrome virus; MOI, multiplicity of infection; ROS, reactive oxygen species; NO, nitric oxide.

    Journal: Frontiers in Immunology

    Article Title: Mitochondrial dysfunction in PRRSV-2-infected macrophages

    doi: 10.3389/fimmu.2025.1670488

    Figure Lengend Snippet: Experimental layout. Lungs were collected from influenza- and PRRSV-negative 8–12-week-old pigs. Mononuclear phagocytes (MNPs) from bronchoalveolar lavage (BAL) and parenchyma (PAR) were enriched using OptiPrep gradient. MNPs were infected with North Carolina (NC) PRRSV-2 strains NC134 and NC174 (MOI = 1) and control media for 12 h. Infected and control porcine alveolar macrophages (PAMs), pulmonary intravascular macrophages (PIMs), monocyte-derived dendritic cells (moDCs), and classical DCs (cDCs) were sorted, and RNA-seq was performed on each cell subset. Additional PAM and PIM sorted cells were used for qPCR and NanoString validations. For other assays, enriched MNPs were plated to allow macrophage adhesion for 2 h. PAMs and PIMs were subsequently infected with different NC PRRSV-2 strains (NC134, NC174, and NC144) or VR2332 prototype strain; uninfected cells were used as negative control. Infected macrophages were used in different assays: mitochondrial function assay (Seahorse Agilent technology) and ROS and NO production assays. Created in BioRender. Crisci, E (2025). https://BioRender.com/65x4hji . PRRSV, porcine reproductive and respiratory syndrome virus; MOI, multiplicity of infection; ROS, reactive oxygen species; NO, nitric oxide.

    Article Snippet: Additionally, the reference strain VR2332 (ATCC strain BIAH-001, GenBank accession ID U87392.3 , L5A.1) was used as the PRRSV-2 prototype strain.

    Techniques: Infection, Control, Derivative Assay, RNA Sequencing, Negative Control, Functional Assay, Virus

    Nucleoplasmic shuttling and expression levels of PRRSV N protein. (A) Nucleoplasmic shuttling and expression of N protein in Marc-145 cells infected with three strains. (B) Differential analysis of the nuclear entry ratio of N protein in Marc-145 cells infected with three strains. “*” indicates a comparison between JXA1 and VR2332 at the same infection time, p < 0.01; “#” indicates a comparison between JXA1-R and VR2332 at the same infection time, p < 0.01.

    Journal: Frontiers in Microbiology

    Article Title: Natural mutations in key NLS amino acids regulate nucleoplasmic shuttling and replication efficiency in PRRSV

    doi: 10.3389/fmicb.2025.1587634

    Figure Lengend Snippet: Nucleoplasmic shuttling and expression levels of PRRSV N protein. (A) Nucleoplasmic shuttling and expression of N protein in Marc-145 cells infected with three strains. (B) Differential analysis of the nuclear entry ratio of N protein in Marc-145 cells infected with three strains. “*” indicates a comparison between JXA1 and VR2332 at the same infection time, p < 0.01; “#” indicates a comparison between JXA1-R and VR2332 at the same infection time, p < 0.01.

    Article Snippet: VR2332 (a classical strain of PRRSV) was purchased from Boehringer Ingelheim Bio-Products Co. Ltd. (Taizhou, China), and JXA1-R (a vaccine strain of HP-PRRSV) was purchased from Qilu Animal Health Products Co., Ltd. (Jinan, China).

    Techniques: Expressing, Infection, Comparison

    Analysis of mRNA expression differences of related proteins among three strains at the same infection time. “*” indicates that JXA1-R and JXA1 were compared with VR2332, respectively, p < 0.01; “#” indicates that JXA1-R was compared with JXA1, p < 0.01. No annotation indicates no statistically significant difference.

    Journal: Frontiers in Microbiology

    Article Title: Natural mutations in key NLS amino acids regulate nucleoplasmic shuttling and replication efficiency in PRRSV

    doi: 10.3389/fmicb.2025.1587634

    Figure Lengend Snippet: Analysis of mRNA expression differences of related proteins among three strains at the same infection time. “*” indicates that JXA1-R and JXA1 were compared with VR2332, respectively, p < 0.01; “#” indicates that JXA1-R was compared with JXA1, p < 0.01. No annotation indicates no statistically significant difference.

    Article Snippet: VR2332 (a classical strain of PRRSV) was purchased from Boehringer Ingelheim Bio-Products Co. Ltd. (Taizhou, China), and JXA1-R (a vaccine strain of HP-PRRSV) was purchased from Qilu Animal Health Products Co., Ltd. (Jinan, China).

    Techniques: Expressing, Infection