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viafluor live cell microtubule stain  (Biotium)


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    Biotium viafluor live cell microtubule stain
    HEK293T cells were transiently transfected with pCMV6 NINL-myc plasmid or vector control plasmid for 48 hours. A. Schematic of the components of the autophagy-lysosome pathway that were evaluated. B. qPCR for NINL RNA. **, p = 0.0022. Student’s t-test. C. LysoTracker Median Fluorescence Intensity (MFI) quantified by flow cytometry plotted relative to vector control. ***, p = 0.0001. Student’s t-test. D. Representative immunoblot for Nlp ( NINL ), Lamp1, LC3B, and B-actin. E. Immunoblot quantification of Lamp1 protein levels normalized to vector controls. Ns, not significant. Student’s t-test. F. Immunoblot quantification of LC3B II/I ratio normalized to vector controls. *, p = 0.0224. Student’s t-test. G. Representative images from live imaging with CytoID. Both vector and NINL expressing cells were treated with rapamycin and chloroquine for 6 hours prior to imaging as a positive control. CytoID (autophagic vesicles) in green, Hoescht (nuclie) in blue, and <t>ViaFluor</t> (cytoskeleton) in red. Scale bar, 10μm. H. Quantification of the number of CytoID puncta per cell, normalized to the cell size (34-80 cells quantified per condition). ***, p = 0.0002; ****, p < 0.0001. Two-way ANOVA. I. Quantification of the average CytoID puncta size per cell. ****, p < 0.0001; **, p = 0.0048; *, p = 0.0151. Two-way ANOVA. Graphs represent mean ± SEM. Results represent 3 independent experiments with 2-3 replicates per condition in each experiment.
    Viafluor Live Cell Microtubule Stain, supplied by Biotium, used in various techniques. Bioz Stars score: 94/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/viafluor live cell microtubule stain/product/Biotium
    Average 94 stars, based on 22 article reviews
    viafluor live cell microtubule stain - by Bioz Stars, 2026-03
    94/100 stars

    Images

    1) Product Images from "Integrative Genomic and Functional Analyses Reveal NINL as a Modulator of Tau Aggregation"

    Article Title: Integrative Genomic and Functional Analyses Reveal NINL as a Modulator of Tau Aggregation

    Journal: bioRxiv

    doi: 10.64898/2025.12.12.694063

    HEK293T cells were transiently transfected with pCMV6 NINL-myc plasmid or vector control plasmid for 48 hours. A. Schematic of the components of the autophagy-lysosome pathway that were evaluated. B. qPCR for NINL RNA. **, p = 0.0022. Student’s t-test. C. LysoTracker Median Fluorescence Intensity (MFI) quantified by flow cytometry plotted relative to vector control. ***, p = 0.0001. Student’s t-test. D. Representative immunoblot for Nlp ( NINL ), Lamp1, LC3B, and B-actin. E. Immunoblot quantification of Lamp1 protein levels normalized to vector controls. Ns, not significant. Student’s t-test. F. Immunoblot quantification of LC3B II/I ratio normalized to vector controls. *, p = 0.0224. Student’s t-test. G. Representative images from live imaging with CytoID. Both vector and NINL expressing cells were treated with rapamycin and chloroquine for 6 hours prior to imaging as a positive control. CytoID (autophagic vesicles) in green, Hoescht (nuclie) in blue, and ViaFluor (cytoskeleton) in red. Scale bar, 10μm. H. Quantification of the number of CytoID puncta per cell, normalized to the cell size (34-80 cells quantified per condition). ***, p = 0.0002; ****, p < 0.0001. Two-way ANOVA. I. Quantification of the average CytoID puncta size per cell. ****, p < 0.0001; **, p = 0.0048; *, p = 0.0151. Two-way ANOVA. Graphs represent mean ± SEM. Results represent 3 independent experiments with 2-3 replicates per condition in each experiment.
    Figure Legend Snippet: HEK293T cells were transiently transfected with pCMV6 NINL-myc plasmid or vector control plasmid for 48 hours. A. Schematic of the components of the autophagy-lysosome pathway that were evaluated. B. qPCR for NINL RNA. **, p = 0.0022. Student’s t-test. C. LysoTracker Median Fluorescence Intensity (MFI) quantified by flow cytometry plotted relative to vector control. ***, p = 0.0001. Student’s t-test. D. Representative immunoblot for Nlp ( NINL ), Lamp1, LC3B, and B-actin. E. Immunoblot quantification of Lamp1 protein levels normalized to vector controls. Ns, not significant. Student’s t-test. F. Immunoblot quantification of LC3B II/I ratio normalized to vector controls. *, p = 0.0224. Student’s t-test. G. Representative images from live imaging with CytoID. Both vector and NINL expressing cells were treated with rapamycin and chloroquine for 6 hours prior to imaging as a positive control. CytoID (autophagic vesicles) in green, Hoescht (nuclie) in blue, and ViaFluor (cytoskeleton) in red. Scale bar, 10μm. H. Quantification of the number of CytoID puncta per cell, normalized to the cell size (34-80 cells quantified per condition). ***, p = 0.0002; ****, p < 0.0001. Two-way ANOVA. I. Quantification of the average CytoID puncta size per cell. ****, p < 0.0001; **, p = 0.0048; *, p = 0.0151. Two-way ANOVA. Graphs represent mean ± SEM. Results represent 3 independent experiments with 2-3 replicates per condition in each experiment.

    Techniques Used: Transfection, Plasmid Preparation, Control, Fluorescence, Flow Cytometry, Western Blot, Imaging, Expressing, Positive Control



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    HEK293T cells were transiently transfected with pCMV6 NINL-myc plasmid or vector control plasmid for 48 hours. A. Schematic of the components of the autophagy-lysosome pathway that were evaluated. B. qPCR for NINL RNA. **, p = 0.0022. Student’s t-test. C. LysoTracker Median Fluorescence Intensity (MFI) quantified by flow cytometry plotted relative to vector control. ***, p = 0.0001. Student’s t-test. D. Representative immunoblot for Nlp ( NINL ), Lamp1, LC3B, and B-actin. E. Immunoblot quantification of Lamp1 protein levels normalized to vector controls. Ns, not significant. Student’s t-test. F. Immunoblot quantification of LC3B II/I ratio normalized to vector controls. *, p = 0.0224. Student’s t-test. G. Representative images from live imaging with CytoID. Both vector and NINL expressing cells were treated with rapamycin and chloroquine for 6 hours prior to imaging as a positive control. CytoID (autophagic vesicles) in green, Hoescht (nuclie) in blue, and <t>ViaFluor</t> (cytoskeleton) in red. Scale bar, 10μm. H. Quantification of the number of CytoID puncta per cell, normalized to the cell size (34-80 cells quantified per condition). ***, p = 0.0002; ****, p < 0.0001. Two-way ANOVA. I. Quantification of the average CytoID puncta size per cell. ****, p < 0.0001; **, p = 0.0048; *, p = 0.0151. Two-way ANOVA. Graphs represent mean ± SEM. Results represent 3 independent experiments with 2-3 replicates per condition in each experiment.
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    HEK293T cells were transiently transfected with pCMV6 NINL-myc plasmid or vector control plasmid for 48 hours. A. Schematic of the components of the autophagy-lysosome pathway that were evaluated. B. qPCR for NINL RNA. **, p = 0.0022. Student’s t-test. C. LysoTracker Median Fluorescence Intensity (MFI) quantified by flow cytometry plotted relative to vector control. ***, p = 0.0001. Student’s t-test. D. Representative immunoblot for Nlp ( NINL ), Lamp1, LC3B, and B-actin. E. Immunoblot quantification of Lamp1 protein levels normalized to vector controls. Ns, not significant. Student’s t-test. F. Immunoblot quantification of LC3B II/I ratio normalized to vector controls. *, p = 0.0224. Student’s t-test. G. Representative images from live imaging with CytoID. Both vector and NINL expressing cells were treated with rapamycin and chloroquine for 6 hours prior to imaging as a positive control. CytoID (autophagic vesicles) in green, Hoescht (nuclie) in blue, and <t>ViaFluor</t> (cytoskeleton) in red. Scale bar, 10μm. H. Quantification of the number of CytoID puncta per cell, normalized to the cell size (34-80 cells quantified per condition). ***, p = 0.0002; ****, p < 0.0001. Two-way ANOVA. I. Quantification of the average CytoID puncta size per cell. ****, p < 0.0001; **, p = 0.0048; *, p = 0.0151. Two-way ANOVA. Graphs represent mean ± SEM. Results represent 3 independent experiments with 2-3 replicates per condition in each experiment.
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    Image Search Results


    HEK293T cells were transiently transfected with pCMV6 NINL-myc plasmid or vector control plasmid for 48 hours. A. Schematic of the components of the autophagy-lysosome pathway that were evaluated. B. qPCR for NINL RNA. **, p = 0.0022. Student’s t-test. C. LysoTracker Median Fluorescence Intensity (MFI) quantified by flow cytometry plotted relative to vector control. ***, p = 0.0001. Student’s t-test. D. Representative immunoblot for Nlp ( NINL ), Lamp1, LC3B, and B-actin. E. Immunoblot quantification of Lamp1 protein levels normalized to vector controls. Ns, not significant. Student’s t-test. F. Immunoblot quantification of LC3B II/I ratio normalized to vector controls. *, p = 0.0224. Student’s t-test. G. Representative images from live imaging with CytoID. Both vector and NINL expressing cells were treated with rapamycin and chloroquine for 6 hours prior to imaging as a positive control. CytoID (autophagic vesicles) in green, Hoescht (nuclie) in blue, and ViaFluor (cytoskeleton) in red. Scale bar, 10μm. H. Quantification of the number of CytoID puncta per cell, normalized to the cell size (34-80 cells quantified per condition). ***, p = 0.0002; ****, p < 0.0001. Two-way ANOVA. I. Quantification of the average CytoID puncta size per cell. ****, p < 0.0001; **, p = 0.0048; *, p = 0.0151. Two-way ANOVA. Graphs represent mean ± SEM. Results represent 3 independent experiments with 2-3 replicates per condition in each experiment.

    Journal: bioRxiv

    Article Title: Integrative Genomic and Functional Analyses Reveal NINL as a Modulator of Tau Aggregation

    doi: 10.64898/2025.12.12.694063

    Figure Lengend Snippet: HEK293T cells were transiently transfected with pCMV6 NINL-myc plasmid or vector control plasmid for 48 hours. A. Schematic of the components of the autophagy-lysosome pathway that were evaluated. B. qPCR for NINL RNA. **, p = 0.0022. Student’s t-test. C. LysoTracker Median Fluorescence Intensity (MFI) quantified by flow cytometry plotted relative to vector control. ***, p = 0.0001. Student’s t-test. D. Representative immunoblot for Nlp ( NINL ), Lamp1, LC3B, and B-actin. E. Immunoblot quantification of Lamp1 protein levels normalized to vector controls. Ns, not significant. Student’s t-test. F. Immunoblot quantification of LC3B II/I ratio normalized to vector controls. *, p = 0.0224. Student’s t-test. G. Representative images from live imaging with CytoID. Both vector and NINL expressing cells were treated with rapamycin and chloroquine for 6 hours prior to imaging as a positive control. CytoID (autophagic vesicles) in green, Hoescht (nuclie) in blue, and ViaFluor (cytoskeleton) in red. Scale bar, 10μm. H. Quantification of the number of CytoID puncta per cell, normalized to the cell size (34-80 cells quantified per condition). ***, p = 0.0002; ****, p < 0.0001. Two-way ANOVA. I. Quantification of the average CytoID puncta size per cell. ****, p < 0.0001; **, p = 0.0048; *, p = 0.0151. Two-way ANOVA. Graphs represent mean ± SEM. Results represent 3 independent experiments with 2-3 replicates per condition in each experiment.

    Article Snippet: Two hours prior to imaging, cells were treated with ViaFluor Live Cell Microtubule Stain (Biotium, Cat #: 70063), and Verapamil HCl (Enzo, Cat #: ENZ-51031) was added at a final concentration of 10μm according to the manufacturer’s instructions.

    Techniques: Transfection, Plasmid Preparation, Control, Fluorescence, Flow Cytometry, Western Blot, Imaging, Expressing, Positive Control