angiogenic factors  (Cusabio)

Journal: Nature Communications

Article Title: Endothelial cell responses in sepsis are attenuated by targeting truncated procalcitonin

doi: 10.1038/s41467-025-68199-x

Figure Lengend Snippet: a Endothelial cells were isolated from murine lungs and subjected to bulk RNA sequencing. Created in BioRender. Brabenec, L. ( https://BioRender.com/mpa96bi ) b MDA plot showing DEGs in color that were more than 2-fold differentially regulated ( p < 0.05) in mice after cecal ligation and puncture (CLP) vs. mice subjected to laparotomy only (sham). Limma uses an empirical Bayes shrinkage method to moderate the standard errors of the estimated log-fold changes, which includes t-tests foreach gene To control for multiple testing the Benjamini and Hochberg method was used. c Principal component analysis of normalized expression values for n = 6 mice/group 18 h after sepsis induction. d Volcano plot representing DEGs in color and labelling of the 10 most up- and down-regulated genes in murine pulmonary endothelium. Limma uses an empirical Bayes shrinkage method to moderate the standard errors of the estimated log-fold changes, which includes t-tests foreach gene To control for multiple testing the Benjamini and Hochberg method was used. e List of the 10 most up- and down-regulated genes including the procalcitonin encoding gene Calca . f Up- and ( g ) down-regulated pathways in murine endothelium in response to polymicrobial sepsis. Enrichment analysis, statistical significance was assessed using adjusted p values (FDR correction) to account for multiple testing. h VEGFa and VEGFc is downregulated in isolated murine pulmonary endothelial cells 18 h after injection of human procalcitonin in mice, n = 3. i Human pulmonary microvascular endothelial cells show increased Calca expression after treatment with sepsis patients serum. This could be abolished by the use of procalcitonin antibody, n = 4 (Healthy), n = 5 (Sepsis), p = 0.0427. j , k Calcrl and Ramp1 show no difference in gene expression, n = 4 (Healthy), n = 5 (Sepsis), p = 0.0035. l VEGFa was downregulated in human pulmonary microvascular endothelial cells when treated with serum of septic patients, n = 4 (Healthy), n = 5 (Sepsis), p = 0.0047. m Expression of genes from LPS-treated human cells in our CLP study. We then took the top 50 up-regulated DEGs from the 8 h LPS study and found that 36 of these were also annotated in our dataset. Mean expression values for these genes from our study were calculated per group and then presented as heatmap with values scaled by row. Cluster 1: All genes in this cluster were up-regulated upon CLP as for LPS 8 h, and almost all DEGs (except ICAM1 ) were reduced in expression after AB treatment. Cluster 2: Genes in this cluster were down-regulated in our dataset upon CPL, but not up-regulated as in the LPS study. Of note, these DEGs were stronger down-regulated after AB treatment. One LPS-DEG gene, VCAM1 , was also up-regulated after CLP, but expressed higher in AB-treated samples compared to non-AB-treated CLP controls. Unpaired t test, data presented as mean ± SEM. p values as indicated. Source data are provided as a Source Data file.

Article Snippet: For mouse samples, the following assays were used: Vegfa (Thermofisher, Mm00437306_m1), Vegfc (Thermofisher, Mm00437310_m1), IL17a (Thermofisher, Mm00439618_m1) IL17f (Thermofisher, Mm00521423_m1) and Gapdh (Thermofisher, Mm99999915_g1).

Techniques: Isolation, RNA Sequencing, Ligation, Control, Expressing, Injection, Gene Expression

Journal: Materials Today Bio

Article Title: Urine-derived stem cells efficiently assemble into micro-bone organoids supported by decellularized bone matrix microparticles for rapidly repairing bone defects through direct filling and paracrine functions

doi: 10.1016/j.mtbio.2025.102533

Figure Lengend Snippet: mRNA-seq reveals the in vitro formation mechanism of uBOs. (A) Schematic diagram of the research design. (B) Volcano plot depicting DEGs profiles in uBOs compared to USCs@DBM-MPs. (C) Heatmap analysis of the DEGs. (D) GO enrichment analysis of osteogenic and angiogenic related biological processes based on upregulated DEGs in uBOs. (E) KEGG analysis of osteogenic and angiogenic related signaling pathways using the upregulated DEGs in uBOs. (F) Heatmap analysis and GSEA analysis of ossification and blood vessel morphogenesis.

Article Snippet: Subsequently, the supernatants were harvested and used to quantify the levels of angiogenic factors ( ANG-5 , VEGF-C ) and osteogenic factors ( IGF-2 , OPG ) using ELISA kits (Elabscience or Cusabio, China), following the manufacturer's instructions.

Techniques: In Vitro, Protein-Protein interactions

Journal: Materials Today Bio

Article Title: Urine-derived stem cells efficiently assemble into micro-bone organoids supported by decellularized bone matrix microparticles for rapidly repairing bone defects through direct filling and paracrine functions

doi: 10.1016/j.mtbio.2025.102533

Figure Lengend Snippet: uBOs can stimulate osteogenesis and angiogenesis through paracrine mechanisms. (A) Schematic diagram of the research design. (B) ELISA was performed to quantify osteogenic cytokines ( OPG , IGF-2 ) and angiogenic cytokines ( VEGF , ANG-5 ) in uBOs-CM, USCs-CM, and CTL-CM. (C) ALP and ARS staining showed the stimulating effects of uBOs-CM, USCs-CM, and CTL-CM on the osteogenic differentiation of BMSCs. Scale bar = 250 μm. (D, E) The expression levels of osteogenic markers ( RUNX2 , OCN ) were assessed by qRT-PCR and WB in each group. (F) Transwell and tube formation assays showed the chemotactic and tube formation stimulating effects of uBOs-CM, USCs-CM, and CTL-CM on HUVECs. Scale bar = 200 μm. (G, H) The expression levels of angiogenic markers ( HIF-1α , VEGF ) were evaluated by qRT-PCR and WB in each group. Data are presented as mean ± SD (n = 3). p-values are calculated using one-way analysis of variance (ANOVA) with Bonferroni post hoc test. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001, and “ns” indicates no significance.

Article Snippet: Subsequently, the supernatants were harvested and used to quantify the levels of angiogenic factors ( ANG-5 , VEGF-C ) and osteogenic factors ( IGF-2 , OPG ) using ELISA kits (Elabscience or Cusabio, China), following the manufacturer's instructions.

Techniques: Enzyme-linked Immunosorbent Assay, Staining, Expressing, Quantitative RT-PCR

Journal: Materials Today Bio

Article Title: Urine-derived stem cells efficiently assemble into micro-bone organoids supported by decellularized bone matrix microparticles for rapidly repairing bone defects through direct filling and paracrine functions

doi: 10.1016/j.mtbio.2025.102533

Figure Lengend Snippet: Evaluation of the in-vivo biological functions of uBOs and its in-vivo tracing. (A, B) After 3 weeks of modeling, immunofluorescence staining was employed to assess the expression levels of osteogenic markers ( RUNX2 , OCN ) and angiogenic markers ( CD31 , VEGF ), and semi-quantitative analysis was performed. Scale bar = 1 mm or 250 μm. (C) qRT-PCR was used to quantitatively detect the gene expression levels of osteogenic markers ( RUNX2 , OCN ) and angiogenic markers ( PDGF-BB , VEGF ) in newly formed bone tissue. (D) After 6 weeks of implantation of uBOs, in-vivo cell tracking was performed using immunohistochemistry. In the uBOs group, more human nuclei-stained cells were detected within the bone trabeculae. Scale bar = 200 μm. Data are presented as mean ± SD (n = 6). p-values are calculated using one-way ANOVA with Bonferroni post hoc test. ∗p < 0.05, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001, and “ns” indicates no significance.

Article Snippet: Subsequently, the supernatants were harvested and used to quantify the levels of angiogenic factors ( ANG-5 , VEGF-C ) and osteogenic factors ( IGF-2 , OPG ) using ELISA kits (Elabscience or Cusabio, China), following the manufacturer's instructions.

Techniques: In Vivo, Immunofluorescence, Staining, Expressing, Quantitative RT-PCR, Gene Expression, Cell Tracking Assay, Immunohistochemistry