Review



vdac2  (Proteintech)


Bioz Verified Symbol Proteintech is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 93
      Buy from Supplier

    Structured Review

    Proteintech vdac2
    VNUT and VDAC associate with newly formed insulin granules (A) Confocal microscopy images of a MIN6 cell immunostained against insulin (green) and VNUT (magenta). (B) Confocal microscopy images of a MIN6 cell immunostained against insulin (green), VDAC (yellow) and VNUT (red). (C) Quantification of the colocalization between VDAC and NPY-Halo. A pulse-chase protocol for Halo labeling was employed where pre-existing granules were labeled with JFX549 (old) and newly synthesized granules with JFX650 (old) (means ± SEM; n = 25 cells from three experiments; Mann-Whitney U-test; *p < 0.05). (D) Confocal microscopy images of mouse islets expressing the AdRIP-proCpepRUSH 30, 60 and 120 min post-biotin addition (200 μM; cyan) and immunostained against VDAC (yellow) and VNUT (red). (E) Confocal microscopy images from a mouse islet showing PLA signal between VNUT and VDAC1 (magenta) and insulin immunoreactivity (cyan). The white region is expanded below. (F) Average number of PLA puncta (using antibodies against VNUT and VDAC1 or VNUT and <t>VDAC2)</t> per insulin-positive cell (single confocal plane; means ± SEM, n = 4 experiments).
    Vdac2, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 43 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/vdac2/bio_rxiv__64898__2026__02__23__707428-37-56-60?v=Proteintech
    Average 93 stars, based on 43 article reviews
    vdac2 - by Bioz Stars, 2026-06
    93/100 stars

    Images

    1) Product Images from "Mitochondria – insulin granule crosstalk controls the early stages of granule maturation"

    Article Title: Mitochondria – insulin granule crosstalk controls the early stages of granule maturation

    Journal: bioRxiv

    doi: 10.64898/2026.02.23.707428

    VNUT and VDAC associate with newly formed insulin granules (A) Confocal microscopy images of a MIN6 cell immunostained against insulin (green) and VNUT (magenta). (B) Confocal microscopy images of a MIN6 cell immunostained against insulin (green), VDAC (yellow) and VNUT (red). (C) Quantification of the colocalization between VDAC and NPY-Halo. A pulse-chase protocol for Halo labeling was employed where pre-existing granules were labeled with JFX549 (old) and newly synthesized granules with JFX650 (old) (means ± SEM; n = 25 cells from three experiments; Mann-Whitney U-test; *p < 0.05). (D) Confocal microscopy images of mouse islets expressing the AdRIP-proCpepRUSH 30, 60 and 120 min post-biotin addition (200 μM; cyan) and immunostained against VDAC (yellow) and VNUT (red). (E) Confocal microscopy images from a mouse islet showing PLA signal between VNUT and VDAC1 (magenta) and insulin immunoreactivity (cyan). The white region is expanded below. (F) Average number of PLA puncta (using antibodies against VNUT and VDAC1 or VNUT and VDAC2) per insulin-positive cell (single confocal plane; means ± SEM, n = 4 experiments).
    Figure Legend Snippet: VNUT and VDAC associate with newly formed insulin granules (A) Confocal microscopy images of a MIN6 cell immunostained against insulin (green) and VNUT (magenta). (B) Confocal microscopy images of a MIN6 cell immunostained against insulin (green), VDAC (yellow) and VNUT (red). (C) Quantification of the colocalization between VDAC and NPY-Halo. A pulse-chase protocol for Halo labeling was employed where pre-existing granules were labeled with JFX549 (old) and newly synthesized granules with JFX650 (old) (means ± SEM; n = 25 cells from three experiments; Mann-Whitney U-test; *p < 0.05). (D) Confocal microscopy images of mouse islets expressing the AdRIP-proCpepRUSH 30, 60 and 120 min post-biotin addition (200 μM; cyan) and immunostained against VDAC (yellow) and VNUT (red). (E) Confocal microscopy images from a mouse islet showing PLA signal between VNUT and VDAC1 (magenta) and insulin immunoreactivity (cyan). The white region is expanded below. (F) Average number of PLA puncta (using antibodies against VNUT and VDAC1 or VNUT and VDAC2) per insulin-positive cell (single confocal plane; means ± SEM, n = 4 experiments).

    Techniques Used: Confocal Microscopy, Pulse Chase, Labeling, Synthesized, MANN-WHITNEY, Expressing



    Similar Products

    vdac2  (Proteintech)
    93
    Proteintech vdac2
    VNUT and VDAC associate with newly formed insulin granules (A) Confocal microscopy images of a MIN6 cell immunostained against insulin (green) and VNUT (magenta). (B) Confocal microscopy images of a MIN6 cell immunostained against insulin (green), VDAC (yellow) and VNUT (red). (C) Quantification of the colocalization between VDAC and NPY-Halo. A pulse-chase protocol for Halo labeling was employed where pre-existing granules were labeled with JFX549 (old) and newly synthesized granules with JFX650 (old) (means ± SEM; n = 25 cells from three experiments; Mann-Whitney U-test; *p < 0.05). (D) Confocal microscopy images of mouse islets expressing the AdRIP-proCpepRUSH 30, 60 and 120 min post-biotin addition (200 μM; cyan) and immunostained against VDAC (yellow) and VNUT (red). (E) Confocal microscopy images from a mouse islet showing PLA signal between VNUT and VDAC1 (magenta) and insulin immunoreactivity (cyan). The white region is expanded below. (F) Average number of PLA puncta (using antibodies against VNUT and VDAC1 or VNUT and <t>VDAC2)</t> per insulin-positive cell (single confocal plane; means ± SEM, n = 4 experiments).
    Vdac2, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/vdac2/bio_rxiv__64898__2026__02__23__707428-37-56-60?v=Proteintech
    Average 93 stars, based on 1 article reviews
    vdac2 - by Bioz Stars, 2026-06
    93/100 stars
    		  Buy from Supplier
    vdac2 9412  (Cell Signaling Technology Inc)
    93
    Cell Signaling Technology Inc vdac2 9412
    VNUT and VDAC associate with newly formed insulin granules (A) Confocal microscopy images of a MIN6 cell immunostained against insulin (green) and VNUT (magenta). (B) Confocal microscopy images of a MIN6 cell immunostained against insulin (green), VDAC (yellow) and VNUT (red). (C) Quantification of the colocalization between VDAC and NPY-Halo. A pulse-chase protocol for Halo labeling was employed where pre-existing granules were labeled with JFX549 (old) and newly synthesized granules with JFX650 (old) (means ± SEM; n = 25 cells from three experiments; Mann-Whitney U-test; *p < 0.05). (D) Confocal microscopy images of mouse islets expressing the AdRIP-proCpepRUSH 30, 60 and 120 min post-biotin addition (200 μM; cyan) and immunostained against VDAC (yellow) and VNUT (red). (E) Confocal microscopy images from a mouse islet showing PLA signal between VNUT and VDAC1 (magenta) and insulin immunoreactivity (cyan). The white region is expanded below. (F) Average number of PLA puncta (using antibodies against VNUT and VDAC1 or VNUT and <t>VDAC2)</t> per insulin-positive cell (single confocal plane; means ± SEM, n = 4 experiments).
    Vdac2 9412, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/vdac2/us12535487-120-8-11?v=Cell+Signaling+Technology+Inc
    Average 93 stars, based on 1 article reviews
    vdac2 9412 - by Bioz Stars, 2026-06
    93/100 stars
    		  Buy from Supplier
    anti vdac2  (Proteintech)
    93
    Proteintech anti vdac2
    VNUT and VDAC associate with newly formed insulin granules (A) Confocal microscopy images of a MIN6 cell immunostained against insulin (green) and VNUT (magenta). (B) Confocal microscopy images of a MIN6 cell immunostained against insulin (green), VDAC (yellow) and VNUT (red). (C) Quantification of the colocalization between VDAC and NPY-Halo. A pulse-chase protocol for Halo labeling was employed where pre-existing granules were labeled with JFX549 (old) and newly synthesized granules with JFX650 (old) (means ± SEM; n = 25 cells from three experiments; Mann-Whitney U-test; *p < 0.05). (D) Confocal microscopy images of mouse islets expressing the AdRIP-proCpepRUSH 30, 60 and 120 min post-biotin addition (200 μM; cyan) and immunostained against VDAC (yellow) and VNUT (red). (E) Confocal microscopy images from a mouse islet showing PLA signal between VNUT and VDAC1 (magenta) and insulin immunoreactivity (cyan). The white region is expanded below. (F) Average number of PLA puncta (using antibodies against VNUT and VDAC1 or VNUT and <t>VDAC2)</t> per insulin-positive cell (single confocal plane; means ± SEM, n = 4 experiments).
    Anti Vdac2, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/vdac2/pm41407227-49-43-47?v=Proteintech
    Average 93 stars, based on 1 article reviews
    anti vdac2 - by Bioz Stars, 2026-06
    93/100 stars
    		  Buy from Supplier
    voltage dependent anion channel 2 vdac2 cst  (Cell Signaling Technology Inc)
    93
    Cell Signaling Technology Inc voltage dependent anion channel 2 vdac2 cst
    VNUT and VDAC associate with newly formed insulin granules (A) Confocal microscopy images of a MIN6 cell immunostained against insulin (green) and VNUT (magenta). (B) Confocal microscopy images of a MIN6 cell immunostained against insulin (green), VDAC (yellow) and VNUT (red). (C) Quantification of the colocalization between VDAC and NPY-Halo. A pulse-chase protocol for Halo labeling was employed where pre-existing granules were labeled with JFX549 (old) and newly synthesized granules with JFX650 (old) (means ± SEM; n = 25 cells from three experiments; Mann-Whitney U-test; *p < 0.05). (D) Confocal microscopy images of mouse islets expressing the AdRIP-proCpepRUSH 30, 60 and 120 min post-biotin addition (200 μM; cyan) and immunostained against VDAC (yellow) and VNUT (red). (E) Confocal microscopy images from a mouse islet showing PLA signal between VNUT and VDAC1 (magenta) and insulin immunoreactivity (cyan). The white region is expanded below. (F) Average number of PLA puncta (using antibodies against VNUT and VDAC1 or VNUT and <t>VDAC2)</t> per insulin-positive cell (single confocal plane; means ± SEM, n = 4 experiments).
    Voltage Dependent Anion Channel 2 Vdac2 Cst, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/vdac2/pm41236875-298-43-49?v=Cell+Signaling+Technology+Inc
    Average 93 stars, based on 1 article reviews
    voltage dependent anion channel 2 vdac2 cst - by Bioz Stars, 2026-06
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    VNUT and VDAC associate with newly formed insulin granules (A) Confocal microscopy images of a MIN6 cell immunostained against insulin (green) and VNUT (magenta). (B) Confocal microscopy images of a MIN6 cell immunostained against insulin (green), VDAC (yellow) and VNUT (red). (C) Quantification of the colocalization between VDAC and NPY-Halo. A pulse-chase protocol for Halo labeling was employed where pre-existing granules were labeled with JFX549 (old) and newly synthesized granules with JFX650 (old) (means ± SEM; n = 25 cells from three experiments; Mann-Whitney U-test; *p < 0.05). (D) Confocal microscopy images of mouse islets expressing the AdRIP-proCpepRUSH 30, 60 and 120 min post-biotin addition (200 μM; cyan) and immunostained against VDAC (yellow) and VNUT (red). (E) Confocal microscopy images from a mouse islet showing PLA signal between VNUT and VDAC1 (magenta) and insulin immunoreactivity (cyan). The white region is expanded below. (F) Average number of PLA puncta (using antibodies against VNUT and VDAC1 or VNUT and VDAC2) per insulin-positive cell (single confocal plane; means ± SEM, n = 4 experiments).

    Journal: bioRxiv

    Article Title: Mitochondria – insulin granule crosstalk controls the early stages of granule maturation

    doi: 10.64898/2026.02.23.707428

    Figure Lengend Snippet: VNUT and VDAC associate with newly formed insulin granules (A) Confocal microscopy images of a MIN6 cell immunostained against insulin (green) and VNUT (magenta). (B) Confocal microscopy images of a MIN6 cell immunostained against insulin (green), VDAC (yellow) and VNUT (red). (C) Quantification of the colocalization between VDAC and NPY-Halo. A pulse-chase protocol for Halo labeling was employed where pre-existing granules were labeled with JFX549 (old) and newly synthesized granules with JFX650 (old) (means ± SEM; n = 25 cells from three experiments; Mann-Whitney U-test; *p < 0.05). (D) Confocal microscopy images of mouse islets expressing the AdRIP-proCpepRUSH 30, 60 and 120 min post-biotin addition (200 μM; cyan) and immunostained against VDAC (yellow) and VNUT (red). (E) Confocal microscopy images from a mouse islet showing PLA signal between VNUT and VDAC1 (magenta) and insulin immunoreactivity (cyan). The white region is expanded below. (F) Average number of PLA puncta (using antibodies against VNUT and VDAC1 or VNUT and VDAC2) per insulin-positive cell (single confocal plane; means ± SEM, n = 4 experiments).

    Article Snippet: The following antibodies and dyes were used in the study: Rab3 monoclonal (catalog no. 107 111, Synaptic Systems, host: mouse, 1:500), TOM20 polyclonal (catalog no. 11802-1-AP, Proteintech, host: Rabbit, 1:400), insulin polyclonal (catalog no. A0564, Dako, host: guinea pig, 1:1000), VDAC (catalog no. MA533205, host: rabbit, 1:200, Invitrogen), VDAC1 (catalog no. 66345-1-lg, Proteintech, host: mouse, 1:200), VDAC2 (catalog no. 66388-1-lg, Proteintech, host: mouse, 1:200), VNUT polyclonal (catalog no. ABN83, Merck, host: guinea pig, 1:200).

    Techniques: Confocal Microscopy, Pulse Chase, Labeling, Synthesized, MANN-WHITNEY, Expressing