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maleimide functionalized monomethyl auristatin e  (MedChemExpress)


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    MedChemExpress maleimide functionalized monomethyl auristatin e
    Schematic representation of <t>monomethyl</t> <t>auristatin</t> <t>E</t> (MMAE) conjugation strategies . A) T22-GFP-H6 is conjugated through its native lysine-amine residues with <t>maleimide-functionalized</t> MMAE at a 1:50 (protein:drug) molar ratio. B) T22-GFP-H6-SORT is site-specifically conjugated with a triglycine-functionalized MMAE molecule via Sortase A-mediated transpeptidation at the C-terminal LPETGLE tag at a 1:1:5 (protein:sortase:drug) molar ratio. C) THIO-T22-GFP-H6 is site-specifically conjugated through its solvent-exposed reactive cysteine with a maleimide-functionalized MMAE molecule at a 1:2 (protein:drug) molar ratio. D) MALDI-TOF spectra show the nanoconjugate populations obtained for each reaction strategy. The unconjugated protein carriers are shown in black (T22-GFP-H6: 30.6 kDa, T22-GFP-H6-SORT: 31.4 kDa and THIO-T22-GFP-H6-MMAE: 30.7 kDa) and the resulting nanoconjugate species are shown in grey for T22-GFP-H6-MMAE (1: 32 kDa, 2: 33.3 kDa, 3: 34.6 kDa, 4: 35.9 kDa, 5: 37.2 kDa, 6: 38.5 kDa, 7: 39.9 kDa, 8: 41.2 kDa, 9: 42.5 kDa), in blue for T22-GFP-H6-SORT (1: 32.3 kDa) and green for THIO-T22-GFP-H6 (1: 32.1 kDa). NC indicates the non-conjugated protein carrier, and the numbered peaks correspond to the different drug-loaded species of each nanoconjugate.
    Maleimide Functionalized Monomethyl Auristatin E, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 95/100, based on 75 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 95 stars, based on 75 article reviews
    maleimide functionalized monomethyl auristatin e - by Bioz Stars, 2026-02
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    Images

    1) Product Images from "Conjugation strategy shapes antitumor efficacy and enables dose-sparing in non-antibody protein nanoconjugates"

    Article Title: Conjugation strategy shapes antitumor efficacy and enables dose-sparing in non-antibody protein nanoconjugates

    Journal: Materials Today Bio

    doi: 10.1016/j.mtbio.2025.102698

    Schematic representation of monomethyl auristatin E (MMAE) conjugation strategies . A) T22-GFP-H6 is conjugated through its native lysine-amine residues with maleimide-functionalized MMAE at a 1:50 (protein:drug) molar ratio. B) T22-GFP-H6-SORT is site-specifically conjugated with a triglycine-functionalized MMAE molecule via Sortase A-mediated transpeptidation at the C-terminal LPETGLE tag at a 1:1:5 (protein:sortase:drug) molar ratio. C) THIO-T22-GFP-H6 is site-specifically conjugated through its solvent-exposed reactive cysteine with a maleimide-functionalized MMAE molecule at a 1:2 (protein:drug) molar ratio. D) MALDI-TOF spectra show the nanoconjugate populations obtained for each reaction strategy. The unconjugated protein carriers are shown in black (T22-GFP-H6: 30.6 kDa, T22-GFP-H6-SORT: 31.4 kDa and THIO-T22-GFP-H6-MMAE: 30.7 kDa) and the resulting nanoconjugate species are shown in grey for T22-GFP-H6-MMAE (1: 32 kDa, 2: 33.3 kDa, 3: 34.6 kDa, 4: 35.9 kDa, 5: 37.2 kDa, 6: 38.5 kDa, 7: 39.9 kDa, 8: 41.2 kDa, 9: 42.5 kDa), in blue for T22-GFP-H6-SORT (1: 32.3 kDa) and green for THIO-T22-GFP-H6 (1: 32.1 kDa). NC indicates the non-conjugated protein carrier, and the numbered peaks correspond to the different drug-loaded species of each nanoconjugate.
    Figure Legend Snippet: Schematic representation of monomethyl auristatin E (MMAE) conjugation strategies . A) T22-GFP-H6 is conjugated through its native lysine-amine residues with maleimide-functionalized MMAE at a 1:50 (protein:drug) molar ratio. B) T22-GFP-H6-SORT is site-specifically conjugated with a triglycine-functionalized MMAE molecule via Sortase A-mediated transpeptidation at the C-terminal LPETGLE tag at a 1:1:5 (protein:sortase:drug) molar ratio. C) THIO-T22-GFP-H6 is site-specifically conjugated through its solvent-exposed reactive cysteine with a maleimide-functionalized MMAE molecule at a 1:2 (protein:drug) molar ratio. D) MALDI-TOF spectra show the nanoconjugate populations obtained for each reaction strategy. The unconjugated protein carriers are shown in black (T22-GFP-H6: 30.6 kDa, T22-GFP-H6-SORT: 31.4 kDa and THIO-T22-GFP-H6-MMAE: 30.7 kDa) and the resulting nanoconjugate species are shown in grey for T22-GFP-H6-MMAE (1: 32 kDa, 2: 33.3 kDa, 3: 34.6 kDa, 4: 35.9 kDa, 5: 37.2 kDa, 6: 38.5 kDa, 7: 39.9 kDa, 8: 41.2 kDa, 9: 42.5 kDa), in blue for T22-GFP-H6-SORT (1: 32.3 kDa) and green for THIO-T22-GFP-H6 (1: 32.1 kDa). NC indicates the non-conjugated protein carrier, and the numbered peaks correspond to the different drug-loaded species of each nanoconjugate.

    Techniques Used: Conjugation Assay, Solvent



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    Schematic representation of <t>monomethyl</t> <t>auristatin</t> <t>E</t> (MMAE) conjugation strategies . A) T22-GFP-H6 is conjugated through its native lysine-amine residues with <t>maleimide-functionalized</t> MMAE at a 1:50 (protein:drug) molar ratio. B) T22-GFP-H6-SORT is site-specifically conjugated with a triglycine-functionalized MMAE molecule via Sortase A-mediated transpeptidation at the C-terminal LPETGLE tag at a 1:1:5 (protein:sortase:drug) molar ratio. C) THIO-T22-GFP-H6 is site-specifically conjugated through its solvent-exposed reactive cysteine with a maleimide-functionalized MMAE molecule at a 1:2 (protein:drug) molar ratio. D) MALDI-TOF spectra show the nanoconjugate populations obtained for each reaction strategy. The unconjugated protein carriers are shown in black (T22-GFP-H6: 30.6 kDa, T22-GFP-H6-SORT: 31.4 kDa and THIO-T22-GFP-H6-MMAE: 30.7 kDa) and the resulting nanoconjugate species are shown in grey for T22-GFP-H6-MMAE (1: 32 kDa, 2: 33.3 kDa, 3: 34.6 kDa, 4: 35.9 kDa, 5: 37.2 kDa, 6: 38.5 kDa, 7: 39.9 kDa, 8: 41.2 kDa, 9: 42.5 kDa), in blue for T22-GFP-H6-SORT (1: 32.3 kDa) and green for THIO-T22-GFP-H6 (1: 32.1 kDa). NC indicates the non-conjugated protein carrier, and the numbered peaks correspond to the different drug-loaded species of each nanoconjugate.
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    Image Search Results


    Schematic representation of monomethyl auristatin E (MMAE) conjugation strategies . A) T22-GFP-H6 is conjugated through its native lysine-amine residues with maleimide-functionalized MMAE at a 1:50 (protein:drug) molar ratio. B) T22-GFP-H6-SORT is site-specifically conjugated with a triglycine-functionalized MMAE molecule via Sortase A-mediated transpeptidation at the C-terminal LPETGLE tag at a 1:1:5 (protein:sortase:drug) molar ratio. C) THIO-T22-GFP-H6 is site-specifically conjugated through its solvent-exposed reactive cysteine with a maleimide-functionalized MMAE molecule at a 1:2 (protein:drug) molar ratio. D) MALDI-TOF spectra show the nanoconjugate populations obtained for each reaction strategy. The unconjugated protein carriers are shown in black (T22-GFP-H6: 30.6 kDa, T22-GFP-H6-SORT: 31.4 kDa and THIO-T22-GFP-H6-MMAE: 30.7 kDa) and the resulting nanoconjugate species are shown in grey for T22-GFP-H6-MMAE (1: 32 kDa, 2: 33.3 kDa, 3: 34.6 kDa, 4: 35.9 kDa, 5: 37.2 kDa, 6: 38.5 kDa, 7: 39.9 kDa, 8: 41.2 kDa, 9: 42.5 kDa), in blue for T22-GFP-H6-SORT (1: 32.3 kDa) and green for THIO-T22-GFP-H6 (1: 32.1 kDa). NC indicates the non-conjugated protein carrier, and the numbered peaks correspond to the different drug-loaded species of each nanoconjugate.

    Journal: Materials Today Bio

    Article Title: Conjugation strategy shapes antitumor efficacy and enables dose-sparing in non-antibody protein nanoconjugates

    doi: 10.1016/j.mtbio.2025.102698

    Figure Lengend Snippet: Schematic representation of monomethyl auristatin E (MMAE) conjugation strategies . A) T22-GFP-H6 is conjugated through its native lysine-amine residues with maleimide-functionalized MMAE at a 1:50 (protein:drug) molar ratio. B) T22-GFP-H6-SORT is site-specifically conjugated with a triglycine-functionalized MMAE molecule via Sortase A-mediated transpeptidation at the C-terminal LPETGLE tag at a 1:1:5 (protein:sortase:drug) molar ratio. C) THIO-T22-GFP-H6 is site-specifically conjugated through its solvent-exposed reactive cysteine with a maleimide-functionalized MMAE molecule at a 1:2 (protein:drug) molar ratio. D) MALDI-TOF spectra show the nanoconjugate populations obtained for each reaction strategy. The unconjugated protein carriers are shown in black (T22-GFP-H6: 30.6 kDa, T22-GFP-H6-SORT: 31.4 kDa and THIO-T22-GFP-H6-MMAE: 30.7 kDa) and the resulting nanoconjugate species are shown in grey for T22-GFP-H6-MMAE (1: 32 kDa, 2: 33.3 kDa, 3: 34.6 kDa, 4: 35.9 kDa, 5: 37.2 kDa, 6: 38.5 kDa, 7: 39.9 kDa, 8: 41.2 kDa, 9: 42.5 kDa), in blue for T22-GFP-H6-SORT (1: 32.3 kDa) and green for THIO-T22-GFP-H6 (1: 32.1 kDa). NC indicates the non-conjugated protein carrier, and the numbered peaks correspond to the different drug-loaded species of each nanoconjugate.

    Article Snippet: Maleimide functionalized monomethyl auristatin E (MC-vc-PAB-MMAE) was purchased at MedChemExpress (HY-15575) and dissolved in anhydrous dimethyl sulfoxide (DMSO).

    Techniques: Conjugation Assay, Solvent