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rabbit polyclonal usp10 antibody  (Bethyl)


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    Structured Review

    Bethyl rabbit polyclonal usp10 antibody
    Chronic stress-pre-incubated cells fail to disassemble polysomes by acute stress U2OS cells were subjected to treatment with 100 μM SA for 1 h after pre-incubation with 10 μM SA, 1 μM Tg (thapsigargin), 60 μM CCCP (carbonyl cyanide m -chlorophenyl hydrazone), or HBSS for 24 h. Unstressed cells (NT) were used as a control. (A) Cells were pulsed with puromycin and emetine for 5 min and lysed. Cell lysates were subjected to western blotting using antibodies for puromycin, p -eIF2α, total eIF2α, G3BP1, G3BP2, caprin1, <t>USP10,</t> and β-actin. Three blots were taken for each of the three biological replicates (independent experiments). The same colors of the circle dot in the graph are from the same independent experiment. Results are mean ± S.E.M. ( n = 3). p values were assessed using a one-way ANOVA ( p ∗ <0.05, p ∗∗ <0.01, p ∗∗∗ <0.001, p ∗∗∗∗ <0.0001). (B–F) Polysome profiles from U2OS cells. NT, black; 1 h SA, red; (B) 24 h pre-incubation of SA, blue; 1 h SA with 24 h pre-incubation of SA, green. (C) 24 h pre-incubation of Tg, blue; 1 h SA with 24 h pre-incubation of Tg, green. (D) 24 h pre-incubation of CCCP, blue; 1 h SA with 24 h pre-incubation of CCCP; green. (E) 24 h pre-incubation of HBSS, blue; 1 h SA with 24 h pre-incubation of HBSS; green. (F) The polysome/monosome ratio was calculated with normalization by each of NT as a control.
    Rabbit Polyclonal Usp10 Antibody, supplied by Bethyl, used in various techniques. Bioz Stars score: 93/100, based on 35 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal usp10 antibody/product/Bethyl
    Average 93 stars, based on 35 article reviews
    rabbit polyclonal usp10 antibody - by Bioz Stars, 2026-05
    93/100 stars

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    1) Product Images from "Chronic stress antagonizes formation of stress granules"

    Article Title: Chronic stress antagonizes formation of stress granules

    Journal: iScience

    doi: 10.1016/j.isci.2025.114556

    Chronic stress-pre-incubated cells fail to disassemble polysomes by acute stress U2OS cells were subjected to treatment with 100 μM SA for 1 h after pre-incubation with 10 μM SA, 1 μM Tg (thapsigargin), 60 μM CCCP (carbonyl cyanide m -chlorophenyl hydrazone), or HBSS for 24 h. Unstressed cells (NT) were used as a control. (A) Cells were pulsed with puromycin and emetine for 5 min and lysed. Cell lysates were subjected to western blotting using antibodies for puromycin, p -eIF2α, total eIF2α, G3BP1, G3BP2, caprin1, USP10, and β-actin. Three blots were taken for each of the three biological replicates (independent experiments). The same colors of the circle dot in the graph are from the same independent experiment. Results are mean ± S.E.M. ( n = 3). p values were assessed using a one-way ANOVA ( p ∗ <0.05, p ∗∗ <0.01, p ∗∗∗ <0.001, p ∗∗∗∗ <0.0001). (B–F) Polysome profiles from U2OS cells. NT, black; 1 h SA, red; (B) 24 h pre-incubation of SA, blue; 1 h SA with 24 h pre-incubation of SA, green. (C) 24 h pre-incubation of Tg, blue; 1 h SA with 24 h pre-incubation of Tg, green. (D) 24 h pre-incubation of CCCP, blue; 1 h SA with 24 h pre-incubation of CCCP; green. (E) 24 h pre-incubation of HBSS, blue; 1 h SA with 24 h pre-incubation of HBSS; green. (F) The polysome/monosome ratio was calculated with normalization by each of NT as a control.
    Figure Legend Snippet: Chronic stress-pre-incubated cells fail to disassemble polysomes by acute stress U2OS cells were subjected to treatment with 100 μM SA for 1 h after pre-incubation with 10 μM SA, 1 μM Tg (thapsigargin), 60 μM CCCP (carbonyl cyanide m -chlorophenyl hydrazone), or HBSS for 24 h. Unstressed cells (NT) were used as a control. (A) Cells were pulsed with puromycin and emetine for 5 min and lysed. Cell lysates were subjected to western blotting using antibodies for puromycin, p -eIF2α, total eIF2α, G3BP1, G3BP2, caprin1, USP10, and β-actin. Three blots were taken for each of the three biological replicates (independent experiments). The same colors of the circle dot in the graph are from the same independent experiment. Results are mean ± S.E.M. ( n = 3). p values were assessed using a one-way ANOVA ( p ∗ <0.05, p ∗∗ <0.01, p ∗∗∗ <0.001, p ∗∗∗∗ <0.0001). (B–F) Polysome profiles from U2OS cells. NT, black; 1 h SA, red; (B) 24 h pre-incubation of SA, blue; 1 h SA with 24 h pre-incubation of SA, green. (C) 24 h pre-incubation of Tg, blue; 1 h SA with 24 h pre-incubation of Tg, green. (D) 24 h pre-incubation of CCCP, blue; 1 h SA with 24 h pre-incubation of CCCP; green. (E) 24 h pre-incubation of HBSS, blue; 1 h SA with 24 h pre-incubation of HBSS; green. (F) The polysome/monosome ratio was calculated with normalization by each of NT as a control.

    Techniques Used: Incubation, Control, Western Blot



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    Chronic stress-pre-incubated cells fail to disassemble polysomes by acute stress U2OS cells were subjected to treatment with 100 μM SA for 1 h after pre-incubation with 10 μM SA, 1 μM Tg (thapsigargin), 60 μM CCCP (carbonyl cyanide m -chlorophenyl hydrazone), or HBSS for 24 h. Unstressed cells (NT) were used as a control. (A) Cells were pulsed with puromycin and emetine for 5 min and lysed. Cell lysates were subjected to western blotting using antibodies for puromycin, p -eIF2α, total eIF2α, G3BP1, G3BP2, caprin1, USP10, and β-actin. Three blots were taken for each of the three biological replicates (independent experiments). The same colors of the circle dot in the graph are from the same independent experiment. Results are mean ± S.E.M. ( n = 3). p values were assessed using a one-way ANOVA ( p ∗ <0.05, p ∗∗ <0.01, p ∗∗∗ <0.001, p ∗∗∗∗ <0.0001). (B–F) Polysome profiles from U2OS cells. NT, black; 1 h SA, red; (B) 24 h pre-incubation of SA, blue; 1 h SA with 24 h pre-incubation of SA, green. (C) 24 h pre-incubation of Tg, blue; 1 h SA with 24 h pre-incubation of Tg, green. (D) 24 h pre-incubation of CCCP, blue; 1 h SA with 24 h pre-incubation of CCCP; green. (E) 24 h pre-incubation of HBSS, blue; 1 h SA with 24 h pre-incubation of HBSS; green. (F) The polysome/monosome ratio was calculated with normalization by each of NT as a control.

    Journal: iScience

    Article Title: Chronic stress antagonizes formation of stress granules

    doi: 10.1016/j.isci.2025.114556

    Figure Lengend Snippet: Chronic stress-pre-incubated cells fail to disassemble polysomes by acute stress U2OS cells were subjected to treatment with 100 μM SA for 1 h after pre-incubation with 10 μM SA, 1 μM Tg (thapsigargin), 60 μM CCCP (carbonyl cyanide m -chlorophenyl hydrazone), or HBSS for 24 h. Unstressed cells (NT) were used as a control. (A) Cells were pulsed with puromycin and emetine for 5 min and lysed. Cell lysates were subjected to western blotting using antibodies for puromycin, p -eIF2α, total eIF2α, G3BP1, G3BP2, caprin1, USP10, and β-actin. Three blots were taken for each of the three biological replicates (independent experiments). The same colors of the circle dot in the graph are from the same independent experiment. Results are mean ± S.E.M. ( n = 3). p values were assessed using a one-way ANOVA ( p ∗ <0.05, p ∗∗ <0.01, p ∗∗∗ <0.001, p ∗∗∗∗ <0.0001). (B–F) Polysome profiles from U2OS cells. NT, black; 1 h SA, red; (B) 24 h pre-incubation of SA, blue; 1 h SA with 24 h pre-incubation of SA, green. (C) 24 h pre-incubation of Tg, blue; 1 h SA with 24 h pre-incubation of Tg, green. (D) 24 h pre-incubation of CCCP, blue; 1 h SA with 24 h pre-incubation of CCCP; green. (E) 24 h pre-incubation of HBSS, blue; 1 h SA with 24 h pre-incubation of HBSS; green. (F) The polysome/monosome ratio was calculated with normalization by each of NT as a control.

    Article Snippet: Rabbit Polyclonal USP10 antibody , Bethyl Laboratories Inc. , A300-900A; RRID: AB_625312.

    Techniques: Incubation, Control, Western Blot