Review

trofinetide (MedChemExpress)

Name: trofinetide
Brand: MedChemExpress
Rating: 94 (4 reviews)

MedChemExpress is a verified supplier

MedChemExpress manufactures this product

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

MedChemExpress trofinetide

Administration of <t>trofinetide</t> rescues spatial memory deficits in APP/PS1 mice. A Mouse age and experimental design. B , C In the Morris water maze behavioral assessment, the distance and time taken to reach the target platform during the platform learning task ( n = 8 per group). D The number of platform crossings during the spatial probe trial ( n = 8 per group). E The percentage of time spent in the target quadrant during the spatial probe trial ( n = 8 per group). F Representative tracking path from the spatial probe trial. * P < 0.05; ** P < 0.01; *** P < 0.001. The distance and time to reach the target were analyzed using two-way ANOVA, while other data were analyzed using one-way ANOVA with Tukey’s post hoc test. Bar graphs represent the mean ± SEM

Trofinetide, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/trofinetide/product/MedChemExpress
Average 94 stars, based on 4 article reviews

trofinetide - by Bioz Stars, 2026-02

94/100 stars

Images

1) Product Images from "Trofinetide Improves Cognitive Function in APP/PS1 Mice by Suppressing Inflammation and Apoptosis"

Article Title: Trofinetide Improves Cognitive Function in APP/PS1 Mice by Suppressing Inflammation and Apoptosis

Journal: Molecular Neurobiology

doi: 10.1007/s12035-025-05500-5

Administration of trofinetide rescues spatial memory deficits in APP/PS1 mice. A Mouse age and experimental design. B , C In the Morris water maze behavioral assessment, the distance and time taken to reach the target platform during the platform learning task ( n = 8 per group). D The number of platform crossings during the spatial probe trial ( n = 8 per group). E The percentage of time spent in the target quadrant during the spatial probe trial ( n = 8 per group). F Representative tracking path from the spatial probe trial. * P < 0.05; ** P < 0.01; *** P < 0.001. The distance and time to reach the target were analyzed using two-way ANOVA, while other data were analyzed using one-way ANOVA with Tukey’s post hoc test. Bar graphs represent the mean ± SEM

Figure Legend Snippet: Administration of trofinetide rescues spatial memory deficits in APP/PS1 mice. A Mouse age and experimental design. B , C In the Morris water maze behavioral assessment, the distance and time taken to reach the target platform during the platform learning task ( n = 8 per group). D The number of platform crossings during the spatial probe trial ( n = 8 per group). E The percentage of time spent in the target quadrant during the spatial probe trial ( n = 8 per group). F Representative tracking path from the spatial probe trial. * P < 0.05; ** P < 0.01; *** P < 0.001. The distance and time to reach the target were analyzed using two-way ANOVA, while other data were analyzed using one-way ANOVA with Tukey’s post hoc test. Bar graphs represent the mean ± SEM

Techniques Used:

Trofinetide reduces Aβ burden in APP/PS1 mice. A Representative images of Aβ deposition in the hippocampus and cortex, stained with anti-Aβ42 antibody. Scale bar: 200 µm. B Quantification of plaque numbers in the hippocampus and cortex was performed using brain sections from 3 mice per group. * P < 0.05; ** P < 0.01. Data are expressed as mean ± SEM

Figure Legend Snippet: Trofinetide reduces Aβ burden in APP/PS1 mice. A Representative images of Aβ deposition in the hippocampus and cortex, stained with anti-Aβ42 antibody. Scale bar: 200 µm. B Quantification of plaque numbers in the hippocampus and cortex was performed using brain sections from 3 mice per group. * P < 0.05; ** P < 0.01. Data are expressed as mean ± SEM

Techniques Used: Staining

Effect of trofinetide on pro-inflammatory cytokines expression and microglial activation in APP/PS1 mice, via PPAR-γ/BACE1/NF-κB signaling pathway. A – C ELISA analysis of TNF-α, IL-1β, and IL-6 levels in mice serum ( n = 6 per group). D – G Immunofluorescence staining of Iba1 in the hippocampus and cortex. Scale bar = 50 μm. Quantification was performed on sections from 3 mice per group. H Representative western blot bands showing the expression of PPAR-γ, BACE1, NF-κB, and p-NF-κB in brain tissue from WT, APP/PS1, and APP/PS1 mice treated with trofinetide. I – K Relative grayscale analysis of PPAR-γ and BACE1 normalized to GAPDH, while p-NF-κB levels were analyzed relative to total NF-κB. Data are presented as mean ± SEM. * P < 0.05, ** P < 0.01, ns = not significant

Figure Legend Snippet: Effect of trofinetide on pro-inflammatory cytokines expression and microglial activation in APP/PS1 mice, via PPAR-γ/BACE1/NF-κB signaling pathway. A – C ELISA analysis of TNF-α, IL-1β, and IL-6 levels in mice serum ( n = 6 per group). D – G Immunofluorescence staining of Iba1 in the hippocampus and cortex. Scale bar = 50 μm. Quantification was performed on sections from 3 mice per group. H Representative western blot bands showing the expression of PPAR-γ, BACE1, NF-κB, and p-NF-κB in brain tissue from WT, APP/PS1, and APP/PS1 mice treated with trofinetide. I – K Relative grayscale analysis of PPAR-γ and BACE1 normalized to GAPDH, while p-NF-κB levels were analyzed relative to total NF-κB. Data are presented as mean ± SEM. * P < 0.05, ** P < 0.01, ns = not significant

Techniques Used: Expressing, Activation Assay, Enzyme-linked Immunosorbent Assay, Immunofluorescence, Staining, Western Blot

Effect of trofinetide on apoptosis protein expression and neuronal damage in APP/PS1 mice. A –D Representative western blot analysis of caspase-3, Bax, Bcl-2, and GAPDH in the cortex of APP/PS1 mice and WT littermates. E – H Immunofluorescence staining of NeuN in the hippocampus and cortex across different groups. Scale bar = 50 μm. Quantification was performed using brain sections from 3 mice per group. * P < 0.05; ** P < 0.01; *** P < 0.001; bar graphs represent mean ± SEM

Figure Legend Snippet: Effect of trofinetide on apoptosis protein expression and neuronal damage in APP/PS1 mice. A –D Representative western blot analysis of caspase-3, Bax, Bcl-2, and GAPDH in the cortex of APP/PS1 mice and WT littermates. E – H Immunofluorescence staining of NeuN in the hippocampus and cortex across different groups. Scale bar = 50 μm. Quantification was performed using brain sections from 3 mice per group. * P < 0.05; ** P < 0.01; *** P < 0.001; bar graphs represent mean ± SEM

Techniques Used: Expressing, Western Blot, Immunofluorescence, Staining

Effects of trofinetide and AβOs treatment on BV2 and HT22 cell viability. A , D Cell viability of BV2 and HT22 cells after 24 h of AβOs treatment. B , E Cell viability of BV2 and HT22 cells after 24 h of trofinetide treatment. C , F Cell viability of BV2 and HT22 cells after 2 h of pre-treatment with trofinetide, followed by 24 h of treatment with trofinetide and AβOs. * P < 0.05, ** P < 0.01. One-way ANOVA with Tukey’s post hoc test. Bar graphs represent mean ± SD

Figure Legend Snippet: Effects of trofinetide and AβOs treatment on BV2 and HT22 cell viability. A , D Cell viability of BV2 and HT22 cells after 24 h of AβOs treatment. B , E Cell viability of BV2 and HT22 cells after 24 h of trofinetide treatment. C , F Cell viability of BV2 and HT22 cells after 2 h of pre-treatment with trofinetide, followed by 24 h of treatment with trofinetide and AβOs. * P < 0.05, ** P < 0.01. One-way ANOVA with Tukey’s post hoc test. Bar graphs represent mean ± SD

Techniques Used:

PPAR-γ/NF-κB pathway and inflammatory cytokine release in BV2 with AβOs and trofinetide treatment. A – C Western blot analysis of PPAR-γ, NF-κB, and p-NF-κB expression in BV2 cells with AβOs and trofinetide treatment. D mRNA expression levels of TNF-α, IL-1β, and IL-6 in BV2 cells after treatment. E ELISA analysis of TNF-α, IL-1β, and IL-6 secretion levels in BV2 cells after treatment. * P < 0.05, ** P < 0.01, **** P < 0.0001. One-way ANOVA with Tukey’s post hoc test. Bar graphs represent mean ± SD

Figure Legend Snippet: PPAR-γ/NF-κB pathway and inflammatory cytokine release in BV2 with AβOs and trofinetide treatment. A – C Western blot analysis of PPAR-γ, NF-κB, and p-NF-κB expression in BV2 cells with AβOs and trofinetide treatment. D mRNA expression levels of TNF-α, IL-1β, and IL-6 in BV2 cells after treatment. E ELISA analysis of TNF-α, IL-1β, and IL-6 secretion levels in BV2 cells after treatment. * P < 0.05, ** P < 0.01, **** P < 0.0001. One-way ANOVA with Tukey’s post hoc test. Bar graphs represent mean ± SD

Techniques Used: Western Blot, Expressing, Enzyme-linked Immunosorbent Assay

Trofinetide inhibits caspase-3 activation and reduces AβOs-induced apoptosis in HT22 cells. A , B Flow cytometry analysis of apoptosis in HT22 cells with AβOs treatments and trofinetide rescue group. C , D Representative western blots and statistical analyses of cleaved caspase-3, Bax, Bcl-2, and GAPDH in AβOs and trofinetide rescue groups. * P < 0.05, ** P < 0.01. One-way ANOVA with Tukey’s post hoc test. Bar graphs represent mean ± SD

Figure Legend Snippet: Trofinetide inhibits caspase-3 activation and reduces AβOs-induced apoptosis in HT22 cells. A , B Flow cytometry analysis of apoptosis in HT22 cells with AβOs treatments and trofinetide rescue group. C , D Representative western blots and statistical analyses of cleaved caspase-3, Bax, Bcl-2, and GAPDH in AβOs and trofinetide rescue groups. * P < 0.05, ** P < 0.01. One-way ANOVA with Tukey’s post hoc test. Bar graphs represent mean ± SD

Techniques Used: Activation Assay, Flow Cytometry, Western Blot

Image Search Results

Journal: Molecular Neurobiology

Article Title: Trofinetide Improves Cognitive Function in APP/PS1 Mice by Suppressing Inflammation and Apoptosis

doi: 10.1007/s12035-025-05500-5

Figure Lengend Snippet: Administration of trofinetide rescues spatial memory deficits in APP/PS1 mice. A Mouse age and experimental design. B , C In the Morris water maze behavioral assessment, the distance and time taken to reach the target platform during the platform learning task ( n = 8 per group). D The number of platform crossings during the spatial probe trial ( n = 8 per group). E The percentage of time spent in the target quadrant during the spatial probe trial ( n = 8 per group). F Representative tracking path from the spatial probe trial. * P < 0.05; ** P < 0.01; *** P < 0.001. The distance and time to reach the target were analyzed using two-way ANOVA, while other data were analyzed using one-way ANOVA with Tukey’s post hoc test. Bar graphs represent the mean ± SEM

Article Snippet: Trofinetide (NNZ-2566, purity > 99%) was purchased from MedChemExpress (NJ, USA), and Aβ42 oligomer powder was obtained from China Peptides Co., Ltd. (Shanghai, China).

Techniques:

Journal: Molecular Neurobiology

Article Title: Trofinetide Improves Cognitive Function in APP/PS1 Mice by Suppressing Inflammation and Apoptosis

doi: 10.1007/s12035-025-05500-5

Figure Lengend Snippet: Trofinetide reduces Aβ burden in APP/PS1 mice. A Representative images of Aβ deposition in the hippocampus and cortex, stained with anti-Aβ42 antibody. Scale bar: 200 µm. B Quantification of plaque numbers in the hippocampus and cortex was performed using brain sections from 3 mice per group. * P < 0.05; ** P < 0.01. Data are expressed as mean ± SEM

Article Snippet: Trofinetide (NNZ-2566, purity > 99%) was purchased from MedChemExpress (NJ, USA), and Aβ42 oligomer powder was obtained from China Peptides Co., Ltd. (Shanghai, China).

Techniques: Staining

Journal: Molecular Neurobiology

Article Title: Trofinetide Improves Cognitive Function in APP/PS1 Mice by Suppressing Inflammation and Apoptosis

doi: 10.1007/s12035-025-05500-5

Figure Lengend Snippet: Effect of trofinetide on pro-inflammatory cytokines expression and microglial activation in APP/PS1 mice, via PPAR-γ/BACE1/NF-κB signaling pathway. A – C ELISA analysis of TNF-α, IL-1β, and IL-6 levels in mice serum ( n = 6 per group). D – G Immunofluorescence staining of Iba1 in the hippocampus and cortex. Scale bar = 50 μm. Quantification was performed on sections from 3 mice per group. H Representative western blot bands showing the expression of PPAR-γ, BACE1, NF-κB, and p-NF-κB in brain tissue from WT, APP/PS1, and APP/PS1 mice treated with trofinetide. I – K Relative grayscale analysis of PPAR-γ and BACE1 normalized to GAPDH, while p-NF-κB levels were analyzed relative to total NF-κB. Data are presented as mean ± SEM. * P < 0.05, ** P < 0.01, ns = not significant

Article Snippet: Trofinetide (NNZ-2566, purity > 99%) was purchased from MedChemExpress (NJ, USA), and Aβ42 oligomer powder was obtained from China Peptides Co., Ltd. (Shanghai, China).

Techniques: Expressing, Activation Assay, Enzyme-linked Immunosorbent Assay, Immunofluorescence, Staining, Western Blot

Journal: Molecular Neurobiology

Article Title: Trofinetide Improves Cognitive Function in APP/PS1 Mice by Suppressing Inflammation and Apoptosis

doi: 10.1007/s12035-025-05500-5

Figure Lengend Snippet: Effect of trofinetide on apoptosis protein expression and neuronal damage in APP/PS1 mice. A –D Representative western blot analysis of caspase-3, Bax, Bcl-2, and GAPDH in the cortex of APP/PS1 mice and WT littermates. E – H Immunofluorescence staining of NeuN in the hippocampus and cortex across different groups. Scale bar = 50 μm. Quantification was performed using brain sections from 3 mice per group. * P < 0.05; ** P < 0.01; *** P < 0.001; bar graphs represent mean ± SEM

Article Snippet: Trofinetide (NNZ-2566, purity > 99%) was purchased from MedChemExpress (NJ, USA), and Aβ42 oligomer powder was obtained from China Peptides Co., Ltd. (Shanghai, China).

Techniques: Expressing, Western Blot, Immunofluorescence, Staining

Journal: Molecular Neurobiology

Article Title: Trofinetide Improves Cognitive Function in APP/PS1 Mice by Suppressing Inflammation and Apoptosis

doi: 10.1007/s12035-025-05500-5

Figure Lengend Snippet: Effects of trofinetide and AβOs treatment on BV2 and HT22 cell viability. A , D Cell viability of BV2 and HT22 cells after 24 h of AβOs treatment. B , E Cell viability of BV2 and HT22 cells after 24 h of trofinetide treatment. C , F Cell viability of BV2 and HT22 cells after 2 h of pre-treatment with trofinetide, followed by 24 h of treatment with trofinetide and AβOs. * P < 0.05, ** P < 0.01. One-way ANOVA with Tukey’s post hoc test. Bar graphs represent mean ± SD

Article Snippet: Trofinetide (NNZ-2566, purity > 99%) was purchased from MedChemExpress (NJ, USA), and Aβ42 oligomer powder was obtained from China Peptides Co., Ltd. (Shanghai, China).

Techniques:

Journal: Molecular Neurobiology

Article Title: Trofinetide Improves Cognitive Function in APP/PS1 Mice by Suppressing Inflammation and Apoptosis

doi: 10.1007/s12035-025-05500-5

Figure Lengend Snippet: PPAR-γ/NF-κB pathway and inflammatory cytokine release in BV2 with AβOs and trofinetide treatment. A – C Western blot analysis of PPAR-γ, NF-κB, and p-NF-κB expression in BV2 cells with AβOs and trofinetide treatment. D mRNA expression levels of TNF-α, IL-1β, and IL-6 in BV2 cells after treatment. E ELISA analysis of TNF-α, IL-1β, and IL-6 secretion levels in BV2 cells after treatment. * P < 0.05, ** P < 0.01, **** P < 0.0001. One-way ANOVA with Tukey’s post hoc test. Bar graphs represent mean ± SD

Article Snippet: Trofinetide (NNZ-2566, purity > 99%) was purchased from MedChemExpress (NJ, USA), and Aβ42 oligomer powder was obtained from China Peptides Co., Ltd. (Shanghai, China).

Techniques: Western Blot, Expressing, Enzyme-linked Immunosorbent Assay

Journal: Molecular Neurobiology

Article Title: Trofinetide Improves Cognitive Function in APP/PS1 Mice by Suppressing Inflammation and Apoptosis

doi: 10.1007/s12035-025-05500-5

Figure Lengend Snippet: Trofinetide inhibits caspase-3 activation and reduces AβOs-induced apoptosis in HT22 cells. A , B Flow cytometry analysis of apoptosis in HT22 cells with AβOs treatments and trofinetide rescue group. C , D Representative western blots and statistical analyses of cleaved caspase-3, Bax, Bcl-2, and GAPDH in AβOs and trofinetide rescue groups. * P < 0.05, ** P < 0.01. One-way ANOVA with Tukey’s post hoc test. Bar graphs represent mean ± SD

Article Snippet: Trofinetide (NNZ-2566, purity > 99%) was purchased from MedChemExpress (NJ, USA), and Aβ42 oligomer powder was obtained from China Peptides Co., Ltd. (Shanghai, China).

Techniques: Activation Assay, Flow Cytometry, Western Blot

Review

Structured Review

Images

1) Product Images from "Trofinetide Improves Cognitive Function in APP/PS1 Mice by Suppressing Inflammation and Apoptosis"

Similar Products

Image Search Results