m tridap  (InvivoGen)

Journal: Experimental & Molecular Medicine

Article Title: The dual roles of peptidoglycans: NOD1 and NOD2 inversely regulate bone metabolism

doi: 10.1038/s12276-025-01522-0

Figure Lengend Snippet: Mice were intraperitoneally administered 1.25 mg/kg of TriDAP or MDP, or PBS, on days 0 and 4. a On day 7, micro-CT images were obtained of the distal femurs of those mice. b BV/TV, Tb.Th, Tb.N and Tb.Sp. were analyzed using a CT analyzer. BV/TV, trabecular bone volume; Tb.Th, trabecular thickness; Tb.N, trabecular number; Tb.Sp., trabecular separation. c Distal femur sections from mice treated with PBS or TriDAP were stained with H&E and photographed with an inverted phase-contrast microscope. d Mice were intraperitoneally administered calcein one day before the first TriDAP administration and after the second TriDAP administration. Resin blocks of those femurs were sectioned. The images were obtained using a fluorescent microscope. e The distance between two labels represents the mineral apposition rate (MAR). Scale bars, 500 μm. f The MAR was quantified using OsteoMeasure. * P < 0.05.

Article Snippet: TriDAP, C12-iE-DAP, C14-Tri-LAN-Gly, MDP and Pam3CSK4 were obtained from InvivoGen.

Techniques: Micro-CT, Staining, Microscopy

Journal: Experimental & Molecular Medicine

Article Title: The dual roles of peptidoglycans: NOD1 and NOD2 inversely regulate bone metabolism

doi: 10.1038/s12276-025-01522-0

Figure Lengend Snippet: a MC3T3-E1 cells were treated with 1 µg/ml TriDAP for 15, 30, 60 or 90 min. The cells were lysed and subjected to western blotting using specific antibodies to IκBα or p65. b MC3T3-E1 cells were treated with 1 µg/ml TriDAP for 15 min and then fixed and stained sequentially with specific antibodies to p65 and Hoechst 33342. Cell images were obtained using a confocal microscope. Scale bar, 20 μm. c , e MC3T3-E1 cells were transfected with an NF-κB-Luc plasmid and treated with 0, 0.1, 1 or 10 µg/ml TriDAP ( c ) or MDP ( e ) for 24 h. The cells were lysed and subjected to a luciferase assay. d MC3T3-E1 cells were treated with 1 µg/ml of MDP for 15, 30, 60 or 90 min. The cells were lysed and subjected to western blotting using specific antibodies to IκBα. f MC3T3-E1 cells were transfected with 6× OSE-Luc plasmids. The cells were pretreated with 10 μM TPCK for 1 h, followed by stimulation with 10 μg/ml TriDAP for an additional 23 h. The cells were lysed and subjected to a luciferase assay. * P < 0.05. n.s. not significant.

Article Snippet: TriDAP, C12-iE-DAP, C14-Tri-LAN-Gly, MDP and Pam3CSK4 were obtained from InvivoGen.

Techniques: Western Blot, Staining, Microscopy, Transfection, Plasmid Preparation, Luciferase