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OriGene tp301733

Tp301733, supplied by OriGene, used in various techniques. Bioz Stars score: 91/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tp301733/pmc10229111-15-9-6?v=OriGene
Average 91 stars, based on 3 article reviews

tp301733 - by Bioz Stars, 2026-08

91/100 stars

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1) Product Images from "Improved isolation of extracellular vesicles by removal of both free proteins and lipoproteins"

Article Title: Improved isolation of extracellular vesicles by removal of both free proteins and lipoproteins

Journal: eLife

doi: 10.7554/eLife.86394

Figure Legend Snippet:

Techniques Used: Enzyme-linked Immunosorbent Assay, Recombinant, Purification, Chromatography

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Journal: eLife

Article Title: Improved isolation of extracellular vesicles by removal of both free proteins and lipoproteins

doi: 10.7554/eLife.86394

Figure Lengend Snippet:

Article Snippet: Peptide, recombinant protein , CD63 , Origene , Cat# TP301733 , .

Techniques: Enzyme-linked Immunosorbent Assay, Recombinant, Purification, Chromatography

$( A ) Levels of CD9, CD63, CD81, ApoB-100, and albumin were measured by Simoa after SEC of 1 ml plasma in each fraction using either Sepharose CL-2B, Sepharose CL-4B, or Sepharose CL-6B resin. ( B ) Extracellular vesicle (EV) yield is calculated in fractions 7–10 for Sepharose CL-2B, Sepharose CL-4B, or Sepharose CL-6B by averaging the ratios of CD9, CD63, and CD81. ( C ) Purity of EVs with respect to lipoproteins or free proteins is calculated by dividing relative EV yield (the average of the ratios of CD9, CD63, and CD81) by levels of ApoB-100 (top) or albumin (bottom). Error bars represent the standard deviation of four columns measured on different days with two technical replicates each. Figure 2—source data 1. Simoa data (protein concentrations) for fractions of SEC columns with different resins. Figure 2—source data 2. Simoa data (protein concentrations) for SEC column with different number of washes.$

Journal: eLife

Article Title: Improved isolation of extracellular vesicles by removal of both free proteins and lipoproteins

doi: 10.7554/eLife.86394

Figure Lengend Snippet: ( A ) Levels of CD9, CD63, CD81, ApoB-100, and albumin were measured by Simoa after SEC of 1 ml plasma in each fraction using either Sepharose CL-2B, Sepharose CL-4B, or Sepharose CL-6B resin. ( B ) Extracellular vesicle (EV) yield is calculated in fractions 7–10 for Sepharose CL-2B, Sepharose CL-4B, or Sepharose CL-6B by averaging the ratios of CD9, CD63, and CD81. ( C ) Purity of EVs with respect to lipoproteins or free proteins is calculated by dividing relative EV yield (the average of the ratios of CD9, CD63, and CD81) by levels of ApoB-100 (top) or albumin (bottom). Error bars represent the standard deviation of four columns measured on different days with two technical replicates each. Figure 2—source data 1. Simoa data (protein concentrations) for fractions of SEC columns with different resins. Figure 2—source data 2. Simoa data (protein concentrations) for SEC column with different number of washes.

Article Snippet: Peptide, recombinant protein , CD63 , Origene , Cat# TP301733 , .

Techniques: Clinical Proteomics, Standard Deviation

$Levels of CD9, CD63, CD81, albumin, and ApoB-100 were measured by Simoa in individual 1 ml fractions (collected from the top) after density gradient centrifugation of plasma using an iodixanol gradient. Error bars represent the standard deviation of two replicates of each measurement. Figure 3—source data 1. Simoa data (protein concentrations) for different density gradient centrifugation fractions.$

Journal: eLife

Article Title: Improved isolation of extracellular vesicles by removal of both free proteins and lipoproteins

doi: 10.7554/eLife.86394

Figure Lengend Snippet: Levels of CD9, CD63, CD81, albumin, and ApoB-100 were measured by Simoa in individual 1 ml fractions (collected from the top) after density gradient centrifugation of plasma using an iodixanol gradient. Error bars represent the standard deviation of two replicates of each measurement. Figure 3—source data 1. Simoa data (protein concentrations) for different density gradient centrifugation fractions.

Article Snippet: Peptide, recombinant protein , CD63 , Origene , Cat# TP301733 , .

Techniques: Gradient Centrifugation, Clinical Proteomics, Standard Deviation

$Levels of CD9, CD63, CD81, ApoB-100 albumin were measured (in duplicate and then averaged) by Simoa to compare extracellular vesicle (EV) isolation from 1 ml plasma using density gradient (DG) centrifugation, SEC, or density gradient centrifugation followed by size exclusion chromatography (DG-SEC). For the DG and DG-SEC condition, fraction 10 was analyzed. Simoa measurements were used to quantify relative EV recovery ( A ), EV/ApoB-100 ratio ( B ), and EV/albumin ratio ( C ).$

Journal: eLife

Article Title: Improved isolation of extracellular vesicles by removal of both free proteins and lipoproteins

doi: 10.7554/eLife.86394

Figure Lengend Snippet: Levels of CD9, CD63, CD81, ApoB-100 albumin were measured (in duplicate and then averaged) by Simoa to compare extracellular vesicle (EV) isolation from 1 ml plasma using density gradient (DG) centrifugation, SEC, or density gradient centrifugation followed by size exclusion chromatography (DG-SEC). For the DG and DG-SEC condition, fraction 10 was analyzed. Simoa measurements were used to quantify relative EV recovery ( A ), EV/ApoB-100 ratio ( B ), and EV/albumin ratio ( C ).

Article Snippet: Peptide, recombinant protein , CD63 , Origene , Cat# TP301733 , .

Techniques: Isolation, Clinical Proteomics, Centrifugation, Gradient Centrifugation, Size-exclusion Chromatography

( A ) Levels of CD9, CD63, CD81, and albumin were measured by Simoa after EV isolation from 1 ml plasma with size exclusion chromatography (SEC) using 0, 1, 2, or 3 in-column 10 ml PBS washes. Error bars represent the standard deviation from two technical replicates. ( B ) Percent recovery of EVs using average of ratios of CD9, CD63, and CD81 in SEC isolation relative to plasma.

Journal: eLife

Article Title: Improved isolation of extracellular vesicles by removal of both free proteins and lipoproteins

doi: 10.7554/eLife.86394

Figure Lengend Snippet: ( A ) Levels of CD9, CD63, CD81, and albumin were measured by Simoa after EV isolation from 1 ml plasma with size exclusion chromatography (SEC) using 0, 1, 2, or 3 in-column 10 ml PBS washes. Error bars represent the standard deviation from two technical replicates. ( B ) Percent recovery of EVs using average of ratios of CD9, CD63, and CD81 in SEC isolation relative to plasma.

Article Snippet: Peptide, recombinant protein , CD63 , Origene , Cat# TP301733 , .

Techniques: Isolation, Clinical Proteomics, Size-exclusion Chromatography, Standard Deviation

$( A ) Schematic of the columns being compared: size exclusion chromatography (SEC) column comprised of 10 ml Sepharose CL-6B, dual-mode chromatography (DMC) columns comprised of 10 ml Sepharose CL-6B SEC resin atop 2 ml Fractogel cation exchange resin, Tri-Mode Chromatography (TMC) columns comprised of 10 ml Sepharose CL-6B SEC resin atop 2 ml 2:1 ratio of 2 ml Fractogel cation exchange resin to Capto Core 700 multimodal chromatography resin. ( B ) Electron microscopy of EVs isolated from plasma using SEC (left), DMC (middle), or TMC (right) columns. EVs indicated with red arrows (among background of lipoproteins). ( C ) EV recovery is calculated for EV isolation from plasma for SEC (fractions 7–10), DMC (fractions 9–12), or TMC (fractions 9–12). Simoa measurements in the designated fractions for CD9, CD63, and CD81 are taken as a ratio relative to measurements of these proteins from diluted plasma and these three ratios are then averaged to calculate recovery. ( D ) Purity of EVs with respect to free proteins is determined by dividing relative EV yield (the average of the ratios of CD9, CD63, and CD81) by relative levels of albumin in each condition. ( E ) Purity of EVs with respect to lipoproteins is determined by dividing relative EV yield (the average of the ratios of CD9, CD63, and CD81) by relative levels of ApoB-100 in each condition. Error bars represent the standard deviation of four column measured on different days with two technical replicates each. Figure 4—source data 1. Simoa data (protein concentrations) for TMC columns with different ratios of resins in the bottom layer. Figure 4—source data 2. Simoa data (protein concentrations) for different fractions of SEC and DMC columns. Figure 4—source data 3. Simoa data (protein concentrations) comparing SEC (fractions 7-10), DMC and TMC columns (fractions 9-12).$

Journal: eLife

Article Title: Improved isolation of extracellular vesicles by removal of both free proteins and lipoproteins

doi: 10.7554/eLife.86394

Figure Lengend Snippet: ( A ) Schematic of the columns being compared: size exclusion chromatography (SEC) column comprised of 10 ml Sepharose CL-6B, dual-mode chromatography (DMC) columns comprised of 10 ml Sepharose CL-6B SEC resin atop 2 ml Fractogel cation exchange resin, Tri-Mode Chromatography (TMC) columns comprised of 10 ml Sepharose CL-6B SEC resin atop 2 ml 2:1 ratio of 2 ml Fractogel cation exchange resin to Capto Core 700 multimodal chromatography resin. ( B ) Electron microscopy of EVs isolated from plasma using SEC (left), DMC (middle), or TMC (right) columns. EVs indicated with red arrows (among background of lipoproteins). ( C ) EV recovery is calculated for EV isolation from plasma for SEC (fractions 7–10), DMC (fractions 9–12), or TMC (fractions 9–12). Simoa measurements in the designated fractions for CD9, CD63, and CD81 are taken as a ratio relative to measurements of these proteins from diluted plasma and these three ratios are then averaged to calculate recovery. ( D ) Purity of EVs with respect to free proteins is determined by dividing relative EV yield (the average of the ratios of CD9, CD63, and CD81) by relative levels of albumin in each condition. ( E ) Purity of EVs with respect to lipoproteins is determined by dividing relative EV yield (the average of the ratios of CD9, CD63, and CD81) by relative levels of ApoB-100 in each condition. Error bars represent the standard deviation of four column measured on different days with two technical replicates each. Figure 4—source data 1. Simoa data (protein concentrations) for TMC columns with different ratios of resins in the bottom layer. Figure 4—source data 2. Simoa data (protein concentrations) for different fractions of SEC and DMC columns. Figure 4—source data 3. Simoa data (protein concentrations) comparing SEC (fractions 7-10), DMC and TMC columns (fractions 9-12).

Article Snippet: Peptide, recombinant protein , CD63 , Origene , Cat# TP301733 , .

Techniques: Size-exclusion Chromatography, Chromatography, Electron Microscopy, Isolation, Clinical Proteomics, Standard Deviation

$Levels of CD9, CD63, CD81, ApoB-100, and albumin were measured (in duplicate and then averaged) by Simoa in extracellular vesicle (EV) samples isolated from 1 ml plasma to compare TMC columns with different volumes and ratios of Fractogel cation exchange resin to Capto Core 700 resin. All conditions describe the bottom layer under a 10 ml Sepharose CL-6B top layer. The 1, 2, or 4 ml volume of the bottom later indicates the volume of the solid resin mixture of Fractogel cation exchange resin and Capto Core 700 resin. The following fractions were collected for each: 8–11 for 1 ml bottom layer, 9–12 for 2 ml bottom layer, and 11–14 for 4 ml bottom layer.$

Journal: eLife

Article Title: Improved isolation of extracellular vesicles by removal of both free proteins and lipoproteins

doi: 10.7554/eLife.86394

Figure Lengend Snippet: Levels of CD9, CD63, CD81, ApoB-100, and albumin were measured (in duplicate and then averaged) by Simoa in extracellular vesicle (EV) samples isolated from 1 ml plasma to compare TMC columns with different volumes and ratios of Fractogel cation exchange resin to Capto Core 700 resin. All conditions describe the bottom layer under a 10 ml Sepharose CL-6B top layer. The 1, 2, or 4 ml volume of the bottom later indicates the volume of the solid resin mixture of Fractogel cation exchange resin and Capto Core 700 resin. The following fractions were collected for each: 8–11 for 1 ml bottom layer, 9–12 for 2 ml bottom layer, and 11–14 for 4 ml bottom layer.

Article Snippet: Peptide, recombinant protein , CD63 , Origene , Cat# TP301733 , .

Techniques: Isolation, Clinical Proteomics

$Levels of CD9, CD63, CD81, ApoB-100, and albumin were measured by Simoa in fractions 7–10 for SEC with 10 ml Sepharose CL-6B column and fractions 7–14 for DMC using a column with 2 ml Fractogel cation exchange bottom layer and 10 ml Sepharose CL-6B top layer. Error bars represent the standard deviation from two technical replicates.$

Journal: eLife

Article Title: Improved isolation of extracellular vesicles by removal of both free proteins and lipoproteins

doi: 10.7554/eLife.86394

Figure Lengend Snippet: Levels of CD9, CD63, CD81, ApoB-100, and albumin were measured by Simoa in fractions 7–10 for SEC with 10 ml Sepharose CL-6B column and fractions 7–14 for DMC using a column with 2 ml Fractogel cation exchange bottom layer and 10 ml Sepharose CL-6B top layer. Error bars represent the standard deviation from two technical replicates.

Article Snippet: Peptide, recombinant protein , CD63 , Origene , Cat# TP301733 , .

Techniques: Standard Deviation

$Levels of CD9, CD63, CD81, ApoB-100, and albumin were measured by Simoa in extracellular vesicle (EV) samples isolated from 1 ml plasma using SEC (fractions 7–10), DMC (fractions 9–12), or TMC columns (fractions 9–12). Error bars represent the standard deviation of four column measured on different days with two technical replicates each.$

Journal: eLife

Article Title: Improved isolation of extracellular vesicles by removal of both free proteins and lipoproteins

doi: 10.7554/eLife.86394

Figure Lengend Snippet: Levels of CD9, CD63, CD81, ApoB-100, and albumin were measured by Simoa in extracellular vesicle (EV) samples isolated from 1 ml plasma using SEC (fractions 7–10), DMC (fractions 9–12), or TMC columns (fractions 9–12). Error bars represent the standard deviation of four column measured on different days with two technical replicates each.

Article Snippet: Peptide, recombinant protein , CD63 , Origene , Cat# TP301733 , .

Techniques: Isolation, Clinical Proteomics, Standard Deviation

$( A ) CAD image of semi-automated SEC stand designed to hold eight columns at once with sliding collection tube holder that allows liquid to drip either into 2 ml collection tubes, or to waste. ( B ) Photograph of stand connected to a Tecan Cavro syringe pump controlled by a Raspberry Pi. ( C ) Simoa comparison of CD9, CD63, CD81, ApoB-100, and albumin when SEC was performed on 16 samples of 1 ml plasma using either manual SEC (8 samples) or SEC on the automated device (8 samples). Each point is the average of two Simoa measurements (technical replicates). Figure 5—source data 1. Simoa data (protein concentrations) comparing manual and automated SEC EV isolation (fractions 7-10).$

Journal: eLife

Article Title: Improved isolation of extracellular vesicles by removal of both free proteins and lipoproteins

doi: 10.7554/eLife.86394

Figure Lengend Snippet: ( A ) CAD image of semi-automated SEC stand designed to hold eight columns at once with sliding collection tube holder that allows liquid to drip either into 2 ml collection tubes, or to waste. ( B ) Photograph of stand connected to a Tecan Cavro syringe pump controlled by a Raspberry Pi. ( C ) Simoa comparison of CD9, CD63, CD81, ApoB-100, and albumin when SEC was performed on 16 samples of 1 ml plasma using either manual SEC (8 samples) or SEC on the automated device (8 samples). Each point is the average of two Simoa measurements (technical replicates). Figure 5—source data 1. Simoa data (protein concentrations) comparing manual and automated SEC EV isolation (fractions 7-10).

Article Snippet: Peptide, recombinant protein , CD63 , Origene , Cat# TP301733 , .

Techniques: Comparison, Clinical Proteomics, Isolation

Journal: eLife

Article Title: Improved isolation of extracellular vesicles by removal of both free proteins and lipoproteins

doi: 10.7554/eLife.86394

Figure Lengend Snippet:

Article Snippet: Peptide, recombinant protein , CD63 , Origene , Cat# TP301733 , .

Techniques: Clinical Proteomics, Enzyme-linked Immunosorbent Assay, Recombinant, Purification, Chromatography

Journal: eLife

Article Title: Improved isolation of extracellular vesicles by removal of both free proteins and lipoproteins

doi: 10.7554/eLife.86394

Figure Lengend Snippet:

Article Snippet: Peptide, recombinant protein , CD63 , Origene , Cat# TP301733 , .

Techniques: Clinical Proteomics, Enzyme-linked Immunosorbent Assay, Recombinant, Purification, Chromatography

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