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anti tomm20  (Proteintech)


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    Structured Review

    Proteintech anti tomm20
    Anti Tomm20, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1322 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tomm20/TOM20+Antibody/pmc12805083-699-7-8
    Average 96 stars, based on 1322 article reviews
    anti tomm20 - by Bioz Stars, 2026-09
    96/100 stars

    Images

    Related Articles

    Incubation:

    Article Title: Vitamin D disrupts NS1-TUFM interaction to suppress pathogenic mitophagy in RSV-induced mitochondrial injury of bronchial epithelial cells.
    Article Snippet: 1 Hunan Provincial Key Laboratory of Pediatric Respirology, Pediatric Medical Center, Hunan Provincial People's Hospital and First Affiliated Hospital of Hunan Normal University, Changsha 410005, P. R. China Hunan Provincial Center for Disease Control and Prevention, Changsha 410005, P. R. China Journal of Microbiology Vol.. 64, No. 1, e2508009 https://doi.org/10.71150/jm.2508009 pISSN 1225–8873 • eISSN 1976–3794 Full article

    Article Title: Neural stem cell-derived exosomal PA2G4 induces ANXA2 degradation to promote mitophagy and alleviate neuronal oxidative stress in cerebral ischemia/reperfusion.
    Article Snippet: 1 Department of Neurology, The Second Affiliated Hospital of Hainan Medical University, Haikou 570311, Hainan, People’s Republic of China 2 Cardiovascular Medicine Intensive Care Unit, The Second Affiliated Hospital of Hainan Medical University, No. 48, Baishuitang Road, Haikou 570311, Hainan, People’s Republic of China Abstract Cerebral ischemia/reperfusion injury (CI/RI) is a common complication of cerebrovascular diseases such as stroke, characterized by mitochondrial dysfunction.. This study investigates the function of proliferation-associated protein 2G4 (PA2G4) released by neural stem cells (NSCs)-derived exosomes (NSC-Exo) in treating middle cerebral artery occlusion/reperfusion (MCAO/R) by regulating mitophagy.. NSC-Exo were extracted and identified.

    other:

    Article Title: USP1 promotes hepatocellular carcinoma progression by modulating mitophagy via stabilizing MCM3 to regulate the Keap1-Nrf2 axis
    Article Snippet: TOMM20 , Proteintech , Cat# 11802-1-AP; RRID: AB_2207530.

    Western Blot:

    Article Title: STIM1-Mitofusin2 interactions tether mitochondria and melanosome contacts that promote melanosome maturation.
    Article Snippet: HaloTag–fused iATPSnFR2 was labeled with Janelia Fluor JFX554 HaloTag Ligand (Promega, HT1030) The following reagents were used: α-MSH (Peptide Institute, 4057-v), forskolin (TGI, F0855), melanin (sigma, M0418), bafilomycin A1 (Adipogen Life Sciences, BVT-0252-C100), biotin (nacarai, 04822-04), BAPTA-AM (nacarai, 3731-24), oligomycin (cayman,11342), 2-DG (FUJIFILM, 046-06483). .. The following primary antibodies were used: myc (Cell Signaling Technology, 2276; 1:1,000), V5 (MBL, PM003; 1:1,000), FLAG (Sigma-Aldrich, F3165; 1:1,000), TRP1 (Santa Cruz Biotechnology, sc-166857; 1:5,000 for immunoblotting, 1:500 for immunofluorescence), ATP5a AR TI CL E IN P RE SS (Abcam, ab14748; 1:5,000), α-tubulin (Sigma-Aldrich, T9026; 1:5,000), OPA1 (BD Biosciences, 612606; 1:5,000), mitofusin1 (BioLegend, 831101; dilution not applicable), mitofusin2 (Santa Cruz Biotechnology, sc-100560; dilution not applicable), tyrosinase (Santa Cruz Biotechnology, sc-20035; 1:1,000), streptavidin–HRP (Vector Laboratories, SA5004; 1:1,000), STIM1 (Cell Signaling Technology, 4916S; 1:1,000 for immunoblotting, 1:500 for immunofluorescence), GAPDH (Santa Cruz Biotechnology, sc-32233; 1:5,000), SDHA (Cell Signaling Technology, 5839S; 1:1,000), and TOMM20 (Proteintech, 11802-1-AP; 1:5,000). ..

    Immunofluorescence:

    Article Title: STIM1-Mitofusin2 interactions tether mitochondria and melanosome contacts that promote melanosome maturation.
    Article Snippet: HaloTag–fused iATPSnFR2 was labeled with Janelia Fluor JFX554 HaloTag Ligand (Promega, HT1030) The following reagents were used: α-MSH (Peptide Institute, 4057-v), forskolin (TGI, F0855), melanin (sigma, M0418), bafilomycin A1 (Adipogen Life Sciences, BVT-0252-C100), biotin (nacarai, 04822-04), BAPTA-AM (nacarai, 3731-24), oligomycin (cayman,11342), 2-DG (FUJIFILM, 046-06483). .. The following primary antibodies were used: myc (Cell Signaling Technology, 2276; 1:1,000), V5 (MBL, PM003; 1:1,000), FLAG (Sigma-Aldrich, F3165; 1:1,000), TRP1 (Santa Cruz Biotechnology, sc-166857; 1:5,000 for immunoblotting, 1:500 for immunofluorescence), ATP5a AR TI CL E IN P RE SS (Abcam, ab14748; 1:5,000), α-tubulin (Sigma-Aldrich, T9026; 1:5,000), OPA1 (BD Biosciences, 612606; 1:5,000), mitofusin1 (BioLegend, 831101; dilution not applicable), mitofusin2 (Santa Cruz Biotechnology, sc-100560; dilution not applicable), tyrosinase (Santa Cruz Biotechnology, sc-20035; 1:1,000), streptavidin–HRP (Vector Laboratories, SA5004; 1:1,000), STIM1 (Cell Signaling Technology, 4916S; 1:1,000 for immunoblotting, 1:500 for immunofluorescence), GAPDH (Santa Cruz Biotechnology, sc-32233; 1:5,000), SDHA (Cell Signaling Technology, 5839S; 1:1,000), and TOMM20 (Proteintech, 11802-1-AP; 1:5,000). ..



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    Effects of PQQ treatment on mitochondrial damage induced by ENR exposure in IEC‐6 cells. (A) Experimental design diagram. (B) Network diagram depicting mitochondrial‐related genes and associated biological pathways. (C–M) Differentially expressed genes related to mitochondrial function. (N–O) Protein levels of <t>Tomm20</t> measured by Western blot analysis. (P) OCR was recorded at baseline and after the sequential injection of oligomycin, FCCP, and a mixture of rotenone and antimycin A. (Q–T) Quantitative analysis of mitochondrial respiration parameters: Basal respiration (Q), ATP‐linked respiration (R), proton leak (S), and maximal respiration (T). Statistical significance among the Control, ENR, and ENR + PQQ groups was assessed using one‐way ANOVA followed by Tukey's multiple‐comparisons test. * p < 0.05, ** p < 0.01, *** p < 0.001.
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    Effects of PQQ treatment on mitochondrial damage induced by ENR exposure in IEC‐6 cells. (A) Experimental design diagram. (B) Network diagram depicting mitochondrial‐related genes and associated biological pathways. (C–M) Differentially expressed genes related to mitochondrial function. (N–O) Protein levels of <t>Tomm20</t> measured by Western blot analysis. (P) OCR was recorded at baseline and after the sequential injection of oligomycin, FCCP, and a mixture of rotenone and antimycin A. (Q–T) Quantitative analysis of mitochondrial respiration parameters: Basal respiration (Q), ATP‐linked respiration (R), proton leak (S), and maximal respiration (T). Statistical significance among the Control, ENR, and ENR + PQQ groups was assessed using one‐way ANOVA followed by Tukey's multiple‐comparisons test. * p < 0.05, ** p < 0.01, *** p < 0.001.
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    Boster Bio anti tomm20
    Effects of PQQ treatment on mitochondrial damage induced by ENR exposure in IEC‐6 cells. (A) Experimental design diagram. (B) Network diagram depicting mitochondrial‐related genes and associated biological pathways. (C–M) Differentially expressed genes related to mitochondrial function. (N–O) Protein levels of <t>Tomm20</t> measured by Western blot analysis. (P) OCR was recorded at baseline and after the sequential injection of oligomycin, FCCP, and a mixture of rotenone and antimycin A. (Q–T) Quantitative analysis of mitochondrial respiration parameters: Basal respiration (Q), ATP‐linked respiration (R), proton leak (S), and maximal respiration (T). Statistical significance among the Control, ENR, and ENR + PQQ groups was assessed using one‐way ANOVA followed by Tukey's multiple‐comparisons test. * p < 0.05, ** p < 0.01, *** p < 0.001.
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    Image Search Results


    Effects of PQQ treatment on mitochondrial damage induced by ENR exposure in IEC‐6 cells. (A) Experimental design diagram. (B) Network diagram depicting mitochondrial‐related genes and associated biological pathways. (C–M) Differentially expressed genes related to mitochondrial function. (N–O) Protein levels of Tomm20 measured by Western blot analysis. (P) OCR was recorded at baseline and after the sequential injection of oligomycin, FCCP, and a mixture of rotenone and antimycin A. (Q–T) Quantitative analysis of mitochondrial respiration parameters: Basal respiration (Q), ATP‐linked respiration (R), proton leak (S), and maximal respiration (T). Statistical significance among the Control, ENR, and ENR + PQQ groups was assessed using one‐way ANOVA followed by Tukey's multiple‐comparisons test. * p < 0.05, ** p < 0.01, *** p < 0.001.

    Journal: Aging Cell

    Article Title: Environmental Enrofloxacin Exposure as a Modifiable Driver of Mitochondria‐Mediated Intestinal Aging and Barrier Dysfunction

    doi: 10.1111/acel.70526

    Figure Lengend Snippet: Effects of PQQ treatment on mitochondrial damage induced by ENR exposure in IEC‐6 cells. (A) Experimental design diagram. (B) Network diagram depicting mitochondrial‐related genes and associated biological pathways. (C–M) Differentially expressed genes related to mitochondrial function. (N–O) Protein levels of Tomm20 measured by Western blot analysis. (P) OCR was recorded at baseline and after the sequential injection of oligomycin, FCCP, and a mixture of rotenone and antimycin A. (Q–T) Quantitative analysis of mitochondrial respiration parameters: Basal respiration (Q), ATP‐linked respiration (R), proton leak (S), and maximal respiration (T). Statistical significance among the Control, ENR, and ENR + PQQ groups was assessed using one‐way ANOVA followed by Tukey's multiple‐comparisons test. * p < 0.05, ** p < 0.01, *** p < 0.001.

    Article Snippet: Intestinal tissues were fixed (4% paraformaldehyde, 15 min), blocked (10% goat serum albumin, 0.4% Triton X‐100), and incubated with primary antibodies from Servicebio: Cdkn1a (GB11153, 1:300), Cdkn2a ( GB151143 , 1:300), Tomm20 ( GB151481 , 1:1000), Grp75 ( GB112273 , 1:650), Cox5a ( GB111676 , 1:500), Hsp60 ( GB112464 , 1:800), Cox4 (GB11250, 1:200), CD3 (GB13014, 1:100), Mucin‐2 (GB11344, 1:500), Occludin ( GB111401 , 1:750), Zo‐1 ( GB115686 , 1:1000), and Claudin‐1 ( GB112543 , 1:1000).

    Techniques: Western Blot, Injection, Control

    Effects of PQQ treatment on mitochondrial function, inflammation, and hypoxia levels in the intestine following ENR exposure. Experimental design diagram (A). Representative immunofluorescence images of mitochondrial function‐related proteins (Hsp60, Cox4, Tomm20, Grp75, Cox5a) and quantification (B–I). Representative immunofluorescence images of CD3 and quantification (J–K). Representative immunofluorescence images of hypoxia markers and quantification (L, M). Data are presented as the mean ± standard error of the mean. Statistical significance among the Control, ENR, and ENR + PQQ groups was assessed using one‐way ANOVA followed by Tukey's multiple‐comparisons test. * p < 0.05, ** p < 0.01, *** p < 0.001.

    Journal: Aging Cell

    Article Title: Environmental Enrofloxacin Exposure as a Modifiable Driver of Mitochondria‐Mediated Intestinal Aging and Barrier Dysfunction

    doi: 10.1111/acel.70526

    Figure Lengend Snippet: Effects of PQQ treatment on mitochondrial function, inflammation, and hypoxia levels in the intestine following ENR exposure. Experimental design diagram (A). Representative immunofluorescence images of mitochondrial function‐related proteins (Hsp60, Cox4, Tomm20, Grp75, Cox5a) and quantification (B–I). Representative immunofluorescence images of CD3 and quantification (J–K). Representative immunofluorescence images of hypoxia markers and quantification (L, M). Data are presented as the mean ± standard error of the mean. Statistical significance among the Control, ENR, and ENR + PQQ groups was assessed using one‐way ANOVA followed by Tukey's multiple‐comparisons test. * p < 0.05, ** p < 0.01, *** p < 0.001.

    Article Snippet: Intestinal tissues were fixed (4% paraformaldehyde, 15 min), blocked (10% goat serum albumin, 0.4% Triton X‐100), and incubated with primary antibodies from Servicebio: Cdkn1a (GB11153, 1:300), Cdkn2a ( GB151143 , 1:300), Tomm20 ( GB151481 , 1:1000), Grp75 ( GB112273 , 1:650), Cox5a ( GB111676 , 1:500), Hsp60 ( GB112464 , 1:800), Cox4 (GB11250, 1:200), CD3 (GB13014, 1:100), Mucin‐2 (GB11344, 1:500), Occludin ( GB111401 , 1:750), Zo‐1 ( GB115686 , 1:1000), and Claudin‐1 ( GB112543 , 1:1000).

    Techniques: Immunofluorescence, Control