tlr5 inhibitor  (MedChemExpress)

Journal: bioRxiv

Article Title: Transcriptome-based lead generation, ligand- and structure-based prioritization and experimental validation of TLR5-activating molecules

doi: 10.64898/2026.02.25.707690

Figure Lengend Snippet:

Article Snippet: TLR5 secreted in the culture supernatants were measured using Human Toll-like Receptor 5, TLR5 ELISA kit (BT LAB, #E0374Hu) according to the manufacturer’s protocol.

Techniques: Control

Journal: bioRxiv

Article Title: Transcriptome-based lead generation, ligand- and structure-based prioritization and experimental validation of TLR5-activating molecules

doi: 10.64898/2026.02.25.707690

Figure Lengend Snippet: This figure shows all the H-bond interactions between robust CMap-generated leads and TLR5 which fall below 3 Å. The minimum 3 H-bond interactions between CMap-generated leads and TLR5 were showed by both Glide module and HADDOCK tool in each complex.

Article Snippet: TLR5 secreted in the culture supernatants were measured using Human Toll-like Receptor 5, TLR5 ELISA kit (BT LAB, #E0374Hu) according to the manufacturer’s protocol.

Techniques: Generated

Journal: bioRxiv

Article Title: Transcriptome-based lead generation, ligand- and structure-based prioritization and experimental validation of TLR5-activating molecules

doi: 10.64898/2026.02.25.707690

Figure Lengend Snippet: (A) CAL 27 cells were incubated with the CMap-generated leads: Cytarabine (200µM, 100µM, 50µM), Azacytidine (200µM, 100µM, 50µM), Penicillin (200µM, 100µM, 50µM), Streptozotocin (400µM, 200µM, 100µM), Piceatannol (400µM, 200µM, 100µM) and flagellin (5ng/ml) for 24h. (B) CAL 27 cells were incubated with the CMap-generated leads: Fenoterol (400µM, 200µM, 100µM), ABT-751 (200µM, 100µM, 50µM), Ganciclovir (200µM, 100µM, 50µM), Kinetin-riboside (100µM, 50µM, 25µM), and flagellin (5ng/ml) for 24h. Bar graph representing the relative expression levels of TLR5 with respect to the untreated control. Fold change is calculated by the signal of the treated sample divided by the signal of the control. If the fold change is >1, TLR5 expression is upregulated whereas fold change <1 is taken as downregulated. All values are expressed as mean ± SEM.

Article Snippet: TLR5 secreted in the culture supernatants were measured using Human Toll-like Receptor 5, TLR5 ELISA kit (BT LAB, #E0374Hu) according to the manufacturer’s protocol.

Techniques: Incubation, Generated, Expressing, Control

Journal: bioRxiv

Article Title: Transcriptome-based lead generation, ligand- and structure-based prioritization and experimental validation of TLR5-activating molecules

doi: 10.64898/2026.02.25.707690

Figure Lengend Snippet:

Article Snippet: TLR5 secreted in the culture supernatants were measured using Human Toll-like Receptor 5, TLR5 ELISA kit (BT LAB, #E0374Hu) according to the manufacturer’s protocol.

Techniques: Control

Journal: bioRxiv

Article Title: Transcriptome-based lead generation, ligand- and structure-based prioritization and experimental validation of TLR5-activating molecules

doi: 10.64898/2026.02.25.707690

Figure Lengend Snippet: This figure shows all the H-bond interactions between robust CMap-generated leads and TLR5 which fall below 3 Å. The minimum 3 H-bond interactions between CMap-generated leads and TLR5 were showed by both Glide module and HADDOCK tool in each complex.

Article Snippet: TLR5 secreted in the culture supernatants were measured using Human Toll-like Receptor 5, TLR5 ELISA kit (BT LAB, #E0374Hu) according to the manufacturer’s protocol.

Techniques: Generated

Journal: bioRxiv

Article Title: Transcriptome-based lead generation, ligand- and structure-based prioritization and experimental validation of TLR5-activating molecules

doi: 10.64898/2026.02.25.707690

Figure Lengend Snippet: (A) CAL 27 cells were incubated with the CMap-generated leads: Cytarabine (200µM, 100µM, 50µM), Azacytidine (200µM, 100µM, 50µM), Penicillin (200µM, 100µM, 50µM), Streptozotocin (400µM, 200µM, 100µM), Piceatannol (400µM, 200µM, 100µM) and flagellin (5ng/ml) for 24h. (B) CAL 27 cells were incubated with the CMap-generated leads: Fenoterol (400µM, 200µM, 100µM), ABT-751 (200µM, 100µM, 50µM), Ganciclovir (200µM, 100µM, 50µM), Kinetin-riboside (100µM, 50µM, 25µM), and flagellin (5ng/ml) for 24h. Bar graph representing the relative expression levels of TLR5 with respect to the untreated control. Fold change is calculated by the signal of the treated sample divided by the signal of the control. If the fold change is >1, TLR5 expression is upregulated whereas fold change <1 is taken as downregulated. All values are expressed as mean ± SEM.

Article Snippet: TLR5 secreted in the culture supernatants were measured using Human Toll-like Receptor 5, TLR5 ELISA kit (BT LAB, #E0374Hu) according to the manufacturer’s protocol.

Techniques: Incubation, Generated, Expressing, Control

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: The effect of Massa Medicata Fermentata on the cytokine secretion of colonic mucosa and visceral sensitivity in rats with IBS-D

doi: 10.3389/fcimb.2025.1616796

Figure Lengend Snippet: Intestinal permeability and expression of flagellin and TLR5 protein in the lamina propria of the mucosa of rats in various groups. FITC-Dextran was used to detect the intestinal permeability of rats and laser confocal microscopy was employed to observe the localization of flagellin and TLR5 proteins of colon three repeated tests. (Adopt Tukey statistical method. Refer to the annotations in the reference image for grouping identification.)

Article Snippet: MMF (China Resources Sanjiu Co., Ltd); glacial acetic acid (10000208 Sinopharm, Inc.); FITC-Dextran (Sigma, Inc.); WB Antibodies: TLR5 Antibody (rabbit source, Proteintech, Inc.), TRIF Antibody (rabbit source, Affinity, Inc.); P-ERK1/2 Antibody (rabbit source, abcam, Inc.); immunofluorescent antibodies: immunofluorescent primary antibody: TLR5 (rabbit source, Three Eagles, Inc.), immunofluorescent primary antibody: Flic (mouse source, Yiqiao, Inc.), and Immunofluorescence secondary antibody: goat anti-rabbit IgGH&L (Cy3) (abcam, Inc.), immunofluorescence secondary antibody: goat anti-mouse IgGH&L (FITC) (abcam, Inc.); Immunohistochemistry antibody: immunohistochemistry primary antibody: TLR5 (rabbit source, proteintech, Inc.), immunohistochemistry primary antibody: TRIF (rabbit source, Affinity, Inc.), the Immunohistochemical primary antibody: p-ERK1/2 (rabbit source abcam), immunohistochemical secondary antibody: HRP-labeled goat anti-rabbit IgG (Biyun Tian, Inc.); Percoll lymphocyte isolate P8370 (Solarbio, Inc.); LPDCs magnetic bead sorting kit (OX62) (MiltenyiBiotec, Inc.) Anti-RatCD103, PE (Bioscience, Inc.); Rat spleen lymphocyte isolate LTS1083PK-200 (TBD, Inc.); CD4 Magnetic Bead Sorting Kit (MiltenyiBiotec, Inc.); CD4MonoclonalAntibody;CCK-8 Kit C0039 (Biotronix, Inc.); Rat Interleukin 12 (IL-12) ELISA Kit, Rat Interferon Gamma (IFN-γ) ELISA Kit, Rat Interleukin 4 (IL-4) ELISA Kit, Rat Interleukin 9 (IL-9) kit, Rat Interleukin 6 (IL-6) ELISA kit, Rat Interleukin 17A (IL-17A) ELISA kit, Rat Transforming Growth Factor β (TGF-β) ELISA kit (Jiangsu Jingmei, Inc.).

Techniques: Permeability, Expressing, Confocal Microscopy

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: The effect of Massa Medicata Fermentata on the cytokine secretion of colonic mucosa and visceral sensitivity in rats with IBS-D

doi: 10.3389/fcimb.2025.1616796

Figure Lengend Snippet: Expression of TLR5, TRIF and p-EPK1/2 in LPDCs of rats in each group. The mRNA expression of TLR5, TRIF, and p-EPK1/2 in LPDCs was detected by qPCR. The expression of TLR5, TRIF, and p-EPK1/2 proteins in LPDCs of rats in each group was detected by WB three repeated tests. (Adopt Tukey statistical method. Refer to the annotations in the reference image for grouping identification.)

Article Snippet: MMF (China Resources Sanjiu Co., Ltd); glacial acetic acid (10000208 Sinopharm, Inc.); FITC-Dextran (Sigma, Inc.); WB Antibodies: TLR5 Antibody (rabbit source, Proteintech, Inc.), TRIF Antibody (rabbit source, Affinity, Inc.); P-ERK1/2 Antibody (rabbit source, abcam, Inc.); immunofluorescent antibodies: immunofluorescent primary antibody: TLR5 (rabbit source, Three Eagles, Inc.), immunofluorescent primary antibody: Flic (mouse source, Yiqiao, Inc.), and Immunofluorescence secondary antibody: goat anti-rabbit IgGH&L (Cy3) (abcam, Inc.), immunofluorescence secondary antibody: goat anti-mouse IgGH&L (FITC) (abcam, Inc.); Immunohistochemistry antibody: immunohistochemistry primary antibody: TLR5 (rabbit source, proteintech, Inc.), immunohistochemistry primary antibody: TRIF (rabbit source, Affinity, Inc.), the Immunohistochemical primary antibody: p-ERK1/2 (rabbit source abcam), immunohistochemical secondary antibody: HRP-labeled goat anti-rabbit IgG (Biyun Tian, Inc.); Percoll lymphocyte isolate P8370 (Solarbio, Inc.); LPDCs magnetic bead sorting kit (OX62) (MiltenyiBiotec, Inc.) Anti-RatCD103, PE (Bioscience, Inc.); Rat spleen lymphocyte isolate LTS1083PK-200 (TBD, Inc.); CD4 Magnetic Bead Sorting Kit (MiltenyiBiotec, Inc.); CD4MonoclonalAntibody;CCK-8 Kit C0039 (Biotronix, Inc.); Rat Interleukin 12 (IL-12) ELISA Kit, Rat Interferon Gamma (IFN-γ) ELISA Kit, Rat Interleukin 4 (IL-4) ELISA Kit, Rat Interleukin 9 (IL-9) kit, Rat Interleukin 6 (IL-6) ELISA kit, Rat Interleukin 17A (IL-17A) ELISA kit, Rat Transforming Growth Factor β (TGF-β) ELISA kit (Jiangsu Jingmei, Inc.).

Techniques: Expressing

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: The effect of Massa Medicata Fermentata on the cytokine secretion of colonic mucosa and visceral sensitivity in rats with IBS-D

doi: 10.3389/fcimb.2025.1616796

Figure Lengend Snippet: Intestinal permeability and expression of flagellin and TLR5 protein in the lamina propria of the mucosa of rats in various groups. FITC-Dextran was used to detect the intestinal permeability of rats and laser confocal microscopy was employed to observe the localization of flagellin and TLR5 proteins of colon three repeated tests. (Adopt Tukey statistical method. Refer to the annotations in the reference image for grouping identification.)

Article Snippet: MMF (China Resources Sanjiu Co., Ltd); glacial acetic acid (10000208 Sinopharm, Inc.); FITC-Dextran (Sigma, Inc.); WB Antibodies: TLR5 Antibody (rabbit source, Proteintech, Inc.), TRIF Antibody (rabbit source, Affinity, Inc.); P-ERK1/2 Antibody (rabbit source, abcam, Inc.); immunofluorescent antibodies: immunofluorescent primary antibody: TLR5 (rabbit source, Three Eagles, Inc.), immunofluorescent primary antibody: Flic (mouse source, Yiqiao, Inc.), and Immunofluorescence secondary antibody: goat anti-rabbit IgGH&L (Cy3) (abcam, Inc.), immunofluorescence secondary antibody: goat anti-mouse IgGH&L (FITC) (abcam, Inc.); Immunohistochemistry antibody: immunohistochemistry primary antibody: TLR5 (rabbit source, proteintech, Inc.), immunohistochemistry primary antibody: TRIF (rabbit source, Affinity, Inc.), the Immunohistochemical primary antibody: p-ERK1/2 (rabbit source abcam), immunohistochemical secondary antibody: HRP-labeled goat anti-rabbit IgG (Biyun Tian, Inc.); Percoll lymphocyte isolate P8370 (Solarbio, Inc.); LPDCs magnetic bead sorting kit (OX62) (MiltenyiBiotec, Inc.) Anti-RatCD103, PE (Bioscience, Inc.); Rat spleen lymphocyte isolate LTS1083PK-200 (TBD, Inc.); CD4 Magnetic Bead Sorting Kit (MiltenyiBiotec, Inc.); CD4MonoclonalAntibody;CCK-8 Kit C0039 (Biotronix, Inc.); Rat Interleukin 12 (IL-12) ELISA Kit, Rat Interferon Gamma (IFN-γ) ELISA Kit, Rat Interleukin 4 (IL-4) ELISA Kit, Rat Interleukin 9 (IL-9) kit, Rat Interleukin 6 (IL-6) ELISA kit, Rat Interleukin 17A (IL-17A) ELISA kit, Rat Transforming Growth Factor β (TGF-β) ELISA kit (Jiangsu Jingmei, Inc.).

Techniques: Permeability, Expressing, Confocal Microscopy

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: The effect of Massa Medicata Fermentata on the cytokine secretion of colonic mucosa and visceral sensitivity in rats with IBS-D

doi: 10.3389/fcimb.2025.1616796

Figure Lengend Snippet: Expression of TLR5, TRIF and p-EPK1/2 in LPDCs of rats in each group. The mRNA expression of TLR5, TRIF, and p-EPK1/2 in LPDCs was detected by qPCR. The expression of TLR5, TRIF, and p-EPK1/2 proteins in LPDCs of rats in each group was detected by WB three repeated tests. (Adopt Tukey statistical method. Refer to the annotations in the reference image for grouping identification.)

Article Snippet: MMF (China Resources Sanjiu Co., Ltd); glacial acetic acid (10000208 Sinopharm, Inc.); FITC-Dextran (Sigma, Inc.); WB Antibodies: TLR5 Antibody (rabbit source, Proteintech, Inc.), TRIF Antibody (rabbit source, Affinity, Inc.); P-ERK1/2 Antibody (rabbit source, abcam, Inc.); immunofluorescent antibodies: immunofluorescent primary antibody: TLR5 (rabbit source, Three Eagles, Inc.), immunofluorescent primary antibody: Flic (mouse source, Yiqiao, Inc.), and Immunofluorescence secondary antibody: goat anti-rabbit IgGH&L (Cy3) (abcam, Inc.), immunofluorescence secondary antibody: goat anti-mouse IgGH&L (FITC) (abcam, Inc.); Immunohistochemistry antibody: immunohistochemistry primary antibody: TLR5 (rabbit source, proteintech, Inc.), immunohistochemistry primary antibody: TRIF (rabbit source, Affinity, Inc.), the Immunohistochemical primary antibody: p-ERK1/2 (rabbit source abcam), immunohistochemical secondary antibody: HRP-labeled goat anti-rabbit IgG (Biyun Tian, Inc.); Percoll lymphocyte isolate P8370 (Solarbio, Inc.); LPDCs magnetic bead sorting kit (OX62) (MiltenyiBiotec, Inc.) Anti-RatCD103, PE (Bioscience, Inc.); Rat spleen lymphocyte isolate LTS1083PK-200 (TBD, Inc.); CD4 Magnetic Bead Sorting Kit (MiltenyiBiotec, Inc.); CD4MonoclonalAntibody;CCK-8 Kit C0039 (Biotronix, Inc.); Rat Interleukin 12 (IL-12) ELISA Kit, Rat Interferon Gamma (IFN-γ) ELISA Kit, Rat Interleukin 4 (IL-4) ELISA Kit, Rat Interleukin 9 (IL-9) kit, Rat Interleukin 6 (IL-6) ELISA kit, Rat Interleukin 17A (IL-17A) ELISA kit, Rat Transforming Growth Factor β (TGF-β) ELISA kit (Jiangsu Jingmei, Inc.).

Techniques: Expressing