anti tsp-1  (Proteintech)

Journal: Investigative Ophthalmology & Visual Science

Article Title: Corneal Nerves Promote Alkali Burn Repair by Modulating Macrophages and Neutrophils via Calcitonin Gene-Related Peptide

doi: 10.1167/iovs.67.4.60

Figure Lengend Snippet: CGRP modulates macrophage function via the cAMP/TSP-1 signaling pathway ( n = 4). (A) Schematic of the cAMP signaling pathway downstream of the CGRP-CALCRL/RAMP1 axis. Activation of adenylyl cyclase (AC) elevates the intracellular level of cAMP. (B) The concentration of cAMP in cell lysates was measured by a competitive ELISA after in vitro stimulation of macrophages with CGRP or co-stimulation of CGRP with SQ22536 for 30 minutes ( n = 4). (C) Bar graph showing quantification of apoptosis in macrophages following in vitro treatment with CGRP alone or in combination with SQ22536 ( n = 4). (D, E) The expression of CD206 and Arg-1 in macrophages after treatment with CGRP or CGRP combined with SQ22536 ( n = 4). (F) Macrophage efferocytosis after CGRP or CGRP + SQ22536 treatment ( n = 4). (G) Volcano plot showing upregulation of thbs1 gene expression in CGRP-treated macrophages. (H, J) Representative micrographs and quantification of increased TSP-1 expression in macrophages following CGRP or CGRP + SQ22536 stimulation in vitro ( n = 4). (I, K) Representative micrographs and quantification of TSP-1+ macrophages in the cornea following 7 days of topical CGRP treatment ( n = 4). (L) Quantification of apoptosis in macrophages treated with TSP-1 in vitro ( n = 4). (M, N) The expression of CD206 and Arg-1 in macrophages after treatment with TSP-1 ( n = 4). (O) Macrophage efferocytosis after CGRP or CGRP + SQ22536 treatment. Data are shown as mean ± SD. ns, not significant; * P < 0.05; ** P < 0.01; *** P < 0.001.

Article Snippet: For TSP-1-induced apoptosis, BMDMs were incubated with RPMI-1640 medium containing 100 nM TSP-1 (HY-P701325; MedChenExpress) for 24 hours.

Techniques: Activation Assay, Concentration Assay, Competitive ELISA, In Vitro, Expressing, Gene Expression

Journal: Oncology Reports

Article Title: Combination of ivermectin and metformin promotes autophagy in MCF-7 cells by inhibiting phosphorylation of the PI3K/AKT/mTOR pathway

doi: 10.3892/or.2026.9136

Figure Lengend Snippet: Western blotting detection of THBS1 protein expression in MCF-7 cells. (A) Representative western blotting image shows the expression of THBS1 protein in MCF-7 cells was downregulated. The molecular weights of THBS1 and β-actin are indicated as 180 and 42 kDa, respectively. (B) Semi-quantification of the western blotting results. IVM, ivermectin (6 µM); MET, metformin (6 mM); MCF-7, human mammary tumor cell line; Ctrl, control; +, with treatment. All data were measured as mean ± SEM by three independent experiments. **P<0.01.

Article Snippet: The present study identified the CDS region of THBS1 from the National Centre for Biotechnology Information (NCBI) database.

Techniques: Western Blot, Expressing, Control

Journal: Oncology Reports

Article Title: Combination of ivermectin and metformin promotes autophagy in MCF-7 cells by inhibiting phosphorylation of the PI3K/AKT/mTOR pathway

doi: 10.3892/or.2026.9136

Figure Lengend Snippet: Overexpression of THBS1 enhances the phosphorylation level of proteins in the PI3K/AKT/mTOR pathway. (A) Schematic diagram of THBS1 overexpression plasmid construction. (B) Detection of THBS1 CDS region amplification by agarose gel electrophoresis. (C) Representative western blotting images and (D) semi-quantification of THBS1 overexpression efficiency in MCF-7 cells. MCF-7, human mammary tumor cell line. All data were measured as mean ± SEM by three independent experiments. **P<0.01.

Article Snippet: The present study identified the CDS region of THBS1 from the National Centre for Biotechnology Information (NCBI) database.

Techniques: Over Expression, Phospho-proteomics, Plasmid Preparation, Amplification, Agarose Gel Electrophoresis, Western Blot