tff3  (Proteintech)

Journal: World Journal of Gastrointestinal Oncology

Article Title: Taurine suppresses gastric intestinal metaplasia in patient-derived organoids and Atp4a -/- mice

doi: 10.4251/wjgo.v18.i2.114161

Figure Lengend Snippet: Taurine increases the mucin 5AC (MUC5AC) level and inhibits the expression of caudal type homeobox 2, MUC2, and Trefoil factor family 3 in Atp4a -/- mice. A: Expression of caudal type homeobox 2 (CDX2), mucin 2 (MUC2), Trefoil factor family 3 (TFF3) and MUC5AC in Atp4a -/- mouse gastric tissues was assessed via immunohistochemistry (IHC) staining ( n = 6); B: IHC scores of CDX2, MUC2, and TFF3 ( n = 6); C: IHC score of MUC5AC ( n = 6). a P < 0.01 vs Atp4a -/- ; b P < 0.01 vs wild-type (WT).

Article Snippet: The gastric tissue sections were dewaxed, rehydrated, and incubated with primary antibodies against MUC5AC (1:200, GTX11335; GeneTex, Irvine, CA, United States), CDX2 (1:100, ab101532; Abcam, Cambridge, United Kingdom), MUC2 (1:2000, 27675-1-AP; Proteintech, Wuhan, China), and TFF3 (1:800, 23277-1-AP; Proteintech).

Techniques: Expressing, Immunohistochemistry

Journal: World Journal of Gastrointestinal Oncology

Article Title: Taurine suppresses gastric intestinal metaplasia in patient-derived organoids and Atp4a -/- mice

doi: 10.4251/wjgo.v18.i2.114161

Figure Lengend Snippet: Taurine downregulates the protein expression of caudal type homeobox 2 and Trefoil factor family 3 in gastric intestinal metaplasia control organoids. A-C: Western blotting was conducted to assess the protein levels of caudal type homeobox 2 (CDX2) and Trefoil factor family 3 (TFF3); D-I: Relative protein levels were quantified. a P < 0.01 vs normal control; b P < 0.05 vs gastric intestinal metaplasia (GIM) control (CON); c P < 0.01 vs GIM CON.

Article Snippet: The gastric tissue sections were dewaxed, rehydrated, and incubated with primary antibodies against MUC5AC (1:200, GTX11335; GeneTex, Irvine, CA, United States), CDX2 (1:100, ab101532; Abcam, Cambridge, United Kingdom), MUC2 (1:2000, 27675-1-AP; Proteintech, Wuhan, China), and TFF3 (1:800, 23277-1-AP; Proteintech).

Techniques: Expressing, Control, Western Blot

Journal: Molecular Medicine Reports

Article Title: ST3Gal1 modulates intestinal barrier function and impacts human ulcerative colitis

doi: 10.3892/mmr.2025.13783

Figure Lengend Snippet: ST3Gal1 expression in inflamed IEC monolayers alters MUC2, TFF3, CDX2, STAT3 and p-STAT3 expression. ST3Gal1 knockdown in ST3Gal1-sh3/IEC significantly increased (A) MUC2, (B) TFF3 and (C) CDX2, but significantly reduced (D) STAT3 mRNA levels in the inflamed IEC monolayer. ST3Gal1 OE in ST3Gal1-OE/IEC significantly reduced (E) MUC2, (F) TFF3 and (G) CDX2 mRNA levels in the inflamed IEC monolayer. (H) ST3Gal1 OE in ST3Gal1-OE/IEC slightly decreased STAT3 mRNA levels in the inflamed IEC monolayer. (I) Western blotting showed no changes in protein expression in non-inflamed knockdown samples. (J) Western blotting showed that MUC2, TFF3 and CDX2 protein levels were increased, and p-STAT3 expression was decreased in the inflamed IEC monolayer comprising ST3Gal1-sh3/IEC. (K) Western blotting showed no notable changes in protein expression in non-inflamed OE samples. (L) Western blotting showed that MUC2, TFF3 and CDX2 protein levels were decreased, and p-STAT3 expression was increased in the inflamed IEC monolayer comprising ST3Gal1-OE/IEC. Data are presented as the mean ± SD. Statistical significance was assessed using one-way analysis of variance followed by Tukey's post-hoc test. *P<0.05 and **P<0.01. IEC, intestinal epithelial cell; sh, short hairpin; Ctr, control; OE, overexpression; MUC2, mucin 2; TFF3, trefoil factor 3; CDX2, homeobox protein CDX-2; p-, phosphorylated.

Article Snippet: Rabbit Polyclonal IgG to TFF3 , Proteintech Group, Inc. , 23277-1-AP , 1:1,000.

Techniques: Expressing, Knockdown, Western Blot, Control, Over Expression

Journal: Molecular Medicine Reports

Article Title: ST3Gal1 modulates intestinal barrier function and impacts human ulcerative colitis

doi: 10.3892/mmr.2025.13783

Figure Lengend Snippet: Comparison of (A) MUC2, (B) TFF3 and (C) CDX2 mRNA levels between non-lesional mucosa (n=44) and lesional mucosa (n=75) from distinct patients with ulcerative colitis. Correlations between (D) MUC2, (E) TFF3 and (F) CDX2 mRNA levels and ST3Gal1 expression. Data in A-C are presented as box and whisker plots, and those in D-F as scatter plots. Statistical significance for group comparisons in A-C was assessed using unpaired t-test. Correlation analyses in D-F were performed using Pearson's correlation coefficient. *P<0.05 and **P<0.01. Data were obtained from the GSE107499 dataset from the Gene Expression Omnibus database. *P<0.05 and **P<0.01. NS, not significant; MUC2, mucin 2; TFF3, trefoil factor 3; CDX2, homeobox protein CDX-2.

Article Snippet: Rabbit Polyclonal IgG to TFF3 , Proteintech Group, Inc. , 23277-1-AP , 1:1,000.

Techniques: Comparison, Expressing, Whisker Assay, Gene Expression

Journal: International Journal of Colorectal Disease

Article Title: Inhibition of TFF3 improves the infiltration and function of CD8 + T cells by downregulating the expression of PD-L1 in colorectal cancer

doi: 10.1007/s00384-025-04964-0

Figure Lengend Snippet: TFF3 has a high secretion level in the colorectal tumor conditional medium. A Gene expression level related to the function of CD8 + T cells (mean ± SD, n = 4). B The differentially expressed proteins between the CM and TCM groups. C Subcellular localization of differentially expressed proteins. D The most differentially expressed extracellular secreted proteins. E. The secretion level of TFF3 (mean ± SD, n = 6)

Article Snippet: We collected the supernatant from NCM460, HT29, and DLD1 cell lines to verify the proteomics results with a human TFF3 ELISA kit (Elabscience).

Techniques: Gene Expression

Journal: International Journal of Colorectal Disease

Article Title: Inhibition of TFF3 improves the infiltration and function of CD8 + T cells by downregulating the expression of PD-L1 in colorectal cancer

doi: 10.1007/s00384-025-04964-0

Figure Lengend Snippet: TFF3 affects the infiltration and function of CD8 + T cells into colorectal tumor. A The immune infiltration in tumor tissues of all patients from the TCGA-COAD database. B The correlation of immune cells from the TCGA-COAD database. C Analysis of immune cell infiltration in tumor samples with varying levels of TFF3 expression. D The level of GZMB and PRF1 based on the different expression of TFF3. E The secretory level of TFF3 after knockdown (mean ± SD, n = 4). F The expression level of GZMB, TNF, and IFNG after the knockdown of TFF3 (mean ± SD, n = 3)

Article Snippet: We collected the supernatant from NCM460, HT29, and DLD1 cell lines to verify the proteomics results with a human TFF3 ELISA kit (Elabscience).

Techniques: Expressing, Knockdown

Journal: International Journal of Colorectal Disease

Article Title: Inhibition of TFF3 improves the infiltration and function of CD8 + T cells by downregulating the expression of PD-L1 in colorectal cancer

doi: 10.1007/s00384-025-04964-0

Figure Lengend Snippet: Knockdown of TFF3 restricted tumor growth. A Tumor growth curve of CT-26 inoculated with control (shNC) or shTFF3 tumor cells (mean ± SEM, n = 6). B The tumor was excised from the mice at the time of euthanasia. C The concentration of TFF3 from tumor interstitial fluid ( n = 3). D The infiltration of CD8 + and CD4 + T cells in the mice tumor. E Flow cytometry analysis of the immune infiltration ( n = 5). F The secretory level of TNF and GZMB in mouse serum (mean ± SD, n = 4)

Article Snippet: We collected the supernatant from NCM460, HT29, and DLD1 cell lines to verify the proteomics results with a human TFF3 ELISA kit (Elabscience).

Techniques: Knockdown, Control, Concentration Assay, Flow Cytometry

Journal: International Journal of Colorectal Disease

Article Title: Inhibition of TFF3 improves the infiltration and function of CD8 + T cells by downregulating the expression of PD-L1 in colorectal cancer

doi: 10.1007/s00384-025-04964-0

Figure Lengend Snippet: Knockdown of TFF3 increases the infiltration of CD8 + T cells in vivo. A Schematic diagram for RNA-seq of tumor cells and T cells sorted from CT-26 tumors formed by shNC and shTFF3 tumor cells. B Detection of CD8 + T cells in tumors with immunofluorescence. Scale bars represent 50 µm (mean ± SD, n = 3). C Volcano plot of differentially expressed genes for TIL-T. D KEGG enrichment analysis of differentially expressed genes for TIL-T

Article Snippet: We collected the supernatant from NCM460, HT29, and DLD1 cell lines to verify the proteomics results with a human TFF3 ELISA kit (Elabscience).

Techniques: Knockdown, In Vivo, RNA Sequencing, Immunofluorescence

Journal: International Journal of Colorectal Disease

Article Title: Inhibition of TFF3 improves the infiltration and function of CD8 + T cells by downregulating the expression of PD-L1 in colorectal cancer

doi: 10.1007/s00384-025-04964-0

Figure Lengend Snippet: Knockdown of TFF3 reduces the expression level of PD-L1 on tumor. A The KEGG enrichment analysis on immune infiltration-related genes was performed by the Cytoscape software. B The expression level of PD-L1 in tumor tissues (mean ± SD, n = 3). C Genes participated in the PD-L1 expression and PD-1 checkpoint pathway in cancer

Article Snippet: We collected the supernatant from NCM460, HT29, and DLD1 cell lines to verify the proteomics results with a human TFF3 ELISA kit (Elabscience).

Techniques: Knockdown, Expressing, Software

Journal: International Journal of Colorectal Disease

Article Title: Inhibition of TFF3 improves the infiltration and function of CD8 + T cells by downregulating the expression of PD-L1 in colorectal cancer

doi: 10.1007/s00384-025-04964-0

Figure Lengend Snippet: Knocking down TFF3 inhibits the AKT/mTOR pathway. A The expression level of p-AKT/AKT (mean ± SD, n = 3). B The expression level of p-mTOR/mTOR (mean ± SD, n = 3). C Validation of the interaction between TFF3 and AKT by Co-IP. D The construction of CEA-CAR. E The apoptosis rates of DLD1 shTFF3 and DLD1 shNC cancer cell lines (mean ± SD, n = 7)

Article Snippet: We collected the supernatant from NCM460, HT29, and DLD1 cell lines to verify the proteomics results with a human TFF3 ELISA kit (Elabscience).

Techniques: Expressing, Biomarker Discovery, Co-Immunoprecipitation Assay