Journal: bioRxiv
Article Title: First identification of camel prion disease in Tataouine, Tunisia: an emerging animal prion disease in North Africa
doi: 10.1101/2025.09.17.675824
Figure Lengend Snippet: Western blot analysis of brain tissues from Tunisian dromedary camels for the detection of the proteinase K-resistant core (PrP res ) of PrP Sc . A) PrP res was detected using the TeSeE TM Western Blot detection kit (Bio-Rad) according to the manufacturer’s instructions. The membrane was probed with the Sha31 monoclonal antibody. Loading order (left to right): classical scrapie control (Ctrl), Tunisian CPrD-positive cases (P81/9–65), and Tunisian CPrD-negative samples (P81/64 and P81/15). All samples, except for P81/64 and P81/15, were diluted 1:4 prior to electrophoresis and loaded at 3.75 mg tissue equivalent per lane. The negative-CPrD samples were loaded at 15 mg tissue equivalent per lane. Approximate molecular weights (expressed in kDa) are reported on the left of the blot. B) Representative Western blot (WB) analysis of different available brain regions from selected positive cases. Brain tissues were analyzed using the ISS WB protocol. Case identifiers are indicated below each blot, while the corresponding brain regions are labeled at the top. Abbreviations: Fr. ctx (frontal cortex), Pre-fr. ctx (prefrontal cortex), Occ. ctx (occipital cortex), Par. ctx (parietal cortex), Cereb. (cerebellum), Temp. ctx (temporal cortex). All membranes were probed with the 12B2 antibody, except for P81/17, which was probed with L42. Tissue equivalents loaded per lane: 2 mg for P81/9; 0.5 mg for P81/13, P81/16, and P81/17. Molecular weights (in kDa) are indicated on the left.
Article Snippet: The western blot by TeSeE kit was performed as recommended by the manufacturer (Bio-Rad), using the monoclonal antibody Sha31 (AbI kit reagent).
Techniques: Western Blot, Membrane, Control, Electrophoresis, Labeling
Bio-Rad is a verified supplier 
