cell lines tera1  (ATCC)

Journal: BMC Cancer

Article Title: Assessment of piRNA biogenesis and function in testicular germ cell tumors and their precursor germ cell neoplasia in situ

doi: 10.1186/s12885-017-3945-6

Figure Lengend Snippet: Knockdown of PL2L60A in TERA1 cell line leads to transcriptional upregulation of transposable elements. a , Transcription level of PL2L60A gene and the following retrotransposons: full-length L1HS/L1PA2/L1PA3s, all Alu elements, AluYa5 subfamily. b , Fraction of TE-deriving small RNAs for full-length L1HS/L1PA2/L1PA3s, all Alu elements, AluYa5 subfamily. c , Chromatin modifications (H3K4me3 and H3K9me3) around all Alu elements, AluYa5 subfamily, and 5’UTR promoter of full-length L1HS/L1PA2/L1PA3. d , DNA methylation level around 5’UTR promoter of full-length L1HS/L1PA2/L1PA3 TEs. The graphs show mean and standard deviation across three biological replicates. P-value for two-tailed Mann-Whitney test is presented (n.s. – non-significant). scRNA – control scrambled RNA, siRNA – anti-PL2L60A siRNA

Article Snippet: Cell line TERA1 (ATCC HTB-105) was purchased from ATTC (USA) and maintained in DMEM/F12 (1:1) (Thermo Fisher Scientific, USA) supplemented with 10% FCS (Thermo Fisher Scientific, USA) [ ].

Techniques: Knockdown, DNA Methylation Assay, Standard Deviation, Two Tailed Test, MANN-WHITNEY, Control

Journal: BMC Cancer

Article Title: Assessment of piRNA biogenesis and function in testicular germ cell tumors and their precursor germ cell neoplasia in situ

doi: 10.1186/s12885-017-3945-6

Figure Lengend Snippet: L1 retrotransposition assay performed with pJM101 vector on Hela (positive control) and TERA1 cell lines. The latter was treated either with siRNA for PIWIL2 or with a scrambled RNA control

Article Snippet: Cell line TERA1 (ATCC HTB-105) was purchased from ATTC (USA) and maintained in DMEM/F12 (1:1) (Thermo Fisher Scientific, USA) supplemented with 10% FCS (Thermo Fisher Scientific, USA) [ ].

Techniques: Plasmid Preparation, Positive Control, Control

Journal: PLoS ONE

Article Title: Intragenic Locus in Human PIWIL2 Gene Shares Promoter and Enhancer Functions

doi: 10.1371/journal.pone.0156454

Figure Lengend Snippet: Relative level of H3K4me1 (Histone 3 lysine 4 monomethylated, enhancer mark), H3K4me3 (Histone 3 lysine 4 trimethylated, active promoter mark), H3K27ac (Histone 3 lysine 27 acetylated, active regulatory element mark) and H3K27me3 (Histone 3 lysine 27 trimethylated, facultative heterochromatin mark) histone modifications assessed by ChIP-PCR with two sets of primer pairs around PIWIL2 canonical promoter in exon 1 and alternative promoters in exons 5 and 7. Results are are shown for four cell lines: TERA1 and NT2D1 –embryonal carcinoma, TCam2 –seminoma, and A549 –lung carcinoma. The negative controls are PIWIL2 intron 8 and an intergenic locus on chromosome 1, the positive control is GAPDH promoter. P-value summary of Mann-Whitney non-paired U test is presented for some peaks (ns–non-significant, *—p-value<0.05, **—p-value<0.01).

Article Snippet: Cell lines used in experiments included TERA1 (ATCC HTB-105, testicular embryonal carcinoma [ ]), NT2/D1 (ATCC CRL-1973, pluripotent testicular embryonal carcinoma [ ]) and A549 (ATCC CCL-185, lung carcinoma [ ]).

Techniques: Positive Control, MANN-WHITNEY

Journal: PLoS ONE

Article Title: Intragenic Locus in Human PIWIL2 Gene Shares Promoter and Enhancer Functions

doi: 10.1371/journal.pone.0156454

Figure Lengend Snippet: Relative promoter activity of SV40 and CMV promoters was assessed in luciferase reporter vectors from in two cell lines: TERA1 and NT2D1 (embryonal carcinoma, panel A). PPP3CC and PHYHIP (panel B) promoters were assessed in luciferase reporter vectors from in four cell lines: TERA1 and NT2D1 –embryonal carcinoma, Tcam2 –seminoma, and A549 –lung carcinoma. P-value summary of Mann-Whitney non-paired U test is presented for peaks showing more than two-fold increase (above horizontal dashed lines) for both forward and reverse orientation of the candidate enhancer compared to “no enhancer” controls (*—p-value<0.05, **—p-value<0.01).

Article Snippet: Cell lines used in experiments included TERA1 (ATCC HTB-105, testicular embryonal carcinoma [ ]), NT2/D1 (ATCC CRL-1973, pluripotent testicular embryonal carcinoma [ ]) and A549 (ATCC CCL-185, lung carcinoma [ ]).

Techniques: Activity Assay, Luciferase, MANN-WHITNEY