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situ probe templates for slit1a  (Addgene inc)


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    Structured Review

    Addgene inc situ probe templates for slit1a
    The trajectories of OSNs in <t>slit1a,</t> slit1b, slit2 , or slit3 knockdown embryos were compared to uninjected sibling controls at 72hpf. (A, B) Neither slit2 or slit3 knockdown induced errors in DZ-projecting BacOR130-1 or CZ-projecting BacOR111-7 OSNs. (C) Neither slit1a or slit1b knockdown induced errors in CZ-projecting BacOR111-7 OSNs. (D, E, F) Similar results were obtained when both slit2 and slit3 were knocked down together or when both slit1a and slit1b were knocked down together. Statistical comparisons were performed using a two-tailed Fisher’s Exact test.
    Situ Probe Templates For Slit1a, supplied by Addgene inc, used in various techniques. Bioz Stars score: 88/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/template+plasmid/Zf+Slit1a+(Plasmid+%2361279)/bio_rxiv__64898__2026__03__19__709608-80-1-6
    Average 88 stars, based on 2 article reviews
    situ probe templates for slit1a - by Bioz Stars, 2026-09
    88/100 stars

    Images

    1) Product Images from "The contribution of Robos to olfactory sensory axon targeting in the olfactory bulb"

    Article Title: The contribution of Robos to olfactory sensory axon targeting in the olfactory bulb

    Journal: bioRxiv

    doi: 10.64898/2026.03.19.709608

    The trajectories of OSNs in slit1a, slit1b, slit2 , or slit3 knockdown embryos were compared to uninjected sibling controls at 72hpf. (A, B) Neither slit2 or slit3 knockdown induced errors in DZ-projecting BacOR130-1 or CZ-projecting BacOR111-7 OSNs. (C) Neither slit1a or slit1b knockdown induced errors in CZ-projecting BacOR111-7 OSNs. (D, E, F) Similar results were obtained when both slit2 and slit3 were knocked down together or when both slit1a and slit1b were knocked down together. Statistical comparisons were performed using a two-tailed Fisher’s Exact test.
    Figure Legend Snippet: The trajectories of OSNs in slit1a, slit1b, slit2 , or slit3 knockdown embryos were compared to uninjected sibling controls at 72hpf. (A, B) Neither slit2 or slit3 knockdown induced errors in DZ-projecting BacOR130-1 or CZ-projecting BacOR111-7 OSNs. (C) Neither slit1a or slit1b knockdown induced errors in CZ-projecting BacOR111-7 OSNs. (D, E, F) Similar results were obtained when both slit2 and slit3 were knocked down together or when both slit1a and slit1b were knocked down together. Statistical comparisons were performed using a two-tailed Fisher’s Exact test.

    Techniques Used: Knockdown, Two Tailed Test

    Fluorescent in situ hybridization was performed at 48 hpf in slit2 F0 knockdown embryos. Hybridization was performed in parallel at the same time as the preparations presented in . Representative 3-µm optical maximum-intensity projections from individual preparations are shown. Upregulation of slit1a and slit3 mRNA in slit2 knockdowns was observed in 3 independent experiments.
    Figure Legend Snippet: Fluorescent in situ hybridization was performed at 48 hpf in slit2 F0 knockdown embryos. Hybridization was performed in parallel at the same time as the preparations presented in . Representative 3-µm optical maximum-intensity projections from individual preparations are shown. Upregulation of slit1a and slit3 mRNA in slit2 knockdowns was observed in 3 independent experiments.

    Techniques Used: In Situ Hybridization, Knockdown, Hybridization

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    Article Snippet: .. The mammalian expression construct for mouse MLXIPL was generated by PCR-amplification from a template plasmid (from the laboratory of Isabelle Leclerc, Addgene #39235) and cloning into pcDNA3 with an N-terminal FLAG epitope tag by Gibson assembly. .. The mammalian expression vector for 3xFLAG-tagged human MGA produced by the laboratory of Guntram Suske was purchased from Addgene (#107715) and modified by overlap extension PCR to remove residues 1052-3065.

    Construct:

    Article Title: Supporting Information for Linear motif specificity in signaling through p38α and ERK2 mitogen- activated protein kinases
    Article Snippet: .. The mammalian expression construct for mouse MLXIPL was generated by PCR-amplification from a template plasmid (from the laboratory of Isabelle Leclerc, Addgene #39235) and cloning into pcDNA3 with an N-terminal FLAG epitope tag by Gibson assembly. .. The mammalian expression vector for 3xFLAG-tagged human MGA produced by the laboratory of Guntram Suske was purchased from Addgene (#107715) and modified by overlap extension PCR to remove residues 1052-3065.

    Generated:

    Article Title: Supporting Information for Linear motif specificity in signaling through p38α and ERK2 mitogen- activated protein kinases
    Article Snippet: .. The mammalian expression construct for mouse MLXIPL was generated by PCR-amplification from a template plasmid (from the laboratory of Isabelle Leclerc, Addgene #39235) and cloning into pcDNA3 with an N-terminal FLAG epitope tag by Gibson assembly. .. The mammalian expression vector for 3xFLAG-tagged human MGA produced by the laboratory of Guntram Suske was purchased from Addgene (#107715) and modified by overlap extension PCR to remove residues 1052-3065.

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    Polymerase Chain Reaction:

    Article Title: Supporting Information for Linear motif specificity in signaling through p38α and ERK2 mitogen- activated protein kinases
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    Article Title: Transposable element–gene chimera cartography, origination and role in enhancing transcriptome plasticity
    Article Snippet: For sequencing, RNA libraries were prepared using the NEBNext Ultra II directional RNA library prep kit for Illumina (New England Biolabs) following the manufacturer’s recommendations. .. cDNA for the flag-tagged Dux gene (Gene ID 664783 ), either with or without the transactivation domain (amino acids 1–178), was PCR-amplified from the template plasmid (Addgene, 138320). ..

    Article Title: Non-uniform temporal scaling of neurogenesis for species-specific dosing of cortical excitatory subtypes
    Article Snippet: The coding sequences of rat (NM_171992.6) and human (NM_053056.3) CCND1 were synthesized and subcloned into a CAG promoter-driven expression plasmid (pCAG-IRES-GFP)( ) (GenScript Inc.). .. Membrane-anchored Achilles was amplified by PCR using primers with a membrane targeting signal sequence from the template plasmid (Addgene #153528) and subcloned into a pCAG plasmid. siRNAs for Ccnd1 knockdown were purchased from QIAGEN: Negative control; Cat# 1027310, rat Ccnd1; Cat# SI04717881 and Cat# SI01496222. ..

    Article Title: Mapping variant effects on anti-tumor hallmarks of primary human T cells with base-editing screens.
    Article Snippet: Single-nucleotide variants (SNVs) in key T cell genes can drive clinical pathologies and could be repurposed to improve cellular cancer immunotherapies.. Here, we perform massively parallel base-editing screens to generate thousands of variants at gene loci annotated with known or potential clinical relevance.. We discover a broad landscape of putative gain-of-function (GOF) and loss-of-function (LOF) mutations, including in PIK3CD and the gene encoding its regulatory subunit, PIK3R1, LCK, SOS1, AKT1 and RHOA.

    Article Title: Heat Stress Triggers Nuclear Invagination and Spatial Compartmentalization of Protein Metabolism.
    Article Snippet: .. For proximity labeling experiments, VIM and NES gene fragments were PCR- amplified from cDNA templates, whereas the APEX2 gene fragment was PCR- amplified from a template plasmid (Addgene #124617). .. These fragments were inserted into the plx304 backbone (Addgene #124617) using seamless cloning.

    Article Title: Transposable element-gene chimera cartography, origination and role in enhancing transcriptome plasticity.
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    Plasmid Preparation:

    Article Title: Supporting Information for Linear motif specificity in signaling through p38α and ERK2 mitogen- activated protein kinases
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    Article Title: Auto-methylation of the histone methyltransferase SetDB1 at its histone-mimic motifs ensures the spreading and maintenance of heterochromatin
    Article Snippet: .. HP1α or SetDB1 fragments were synthesized by Twist bioscience, pEF1α was amplified from a template plasmid (Addgene, 78099), BGHpA was amplified from the pcDNA5/FRT/TO vector, and the puromycin resistance gene was amplified from the PiggyBac vector backbone. ..

    Article Title: Transposable element–gene chimera cartography, origination and role in enhancing transcriptome plasticity
    Article Snippet: For sequencing, RNA libraries were prepared using the NEBNext Ultra II directional RNA library prep kit for Illumina (New England Biolabs) following the manufacturer’s recommendations. .. cDNA for the flag-tagged Dux gene (Gene ID 664783 ), either with or without the transactivation domain (amino acids 1–178), was PCR-amplified from the template plasmid (Addgene, 138320). ..

    Article Title: Non-uniform temporal scaling of neurogenesis for species-specific dosing of cortical excitatory subtypes
    Article Snippet: The coding sequences of rat (NM_171992.6) and human (NM_053056.3) CCND1 were synthesized and subcloned into a CAG promoter-driven expression plasmid (pCAG-IRES-GFP)( ) (GenScript Inc.). .. Membrane-anchored Achilles was amplified by PCR using primers with a membrane targeting signal sequence from the template plasmid (Addgene #153528) and subcloned into a pCAG plasmid. siRNAs for Ccnd1 knockdown were purchased from QIAGEN: Negative control; Cat# 1027310, rat Ccnd1; Cat# SI04717881 and Cat# SI01496222. ..

    Article Title: Mapping variant effects on anti-tumor hallmarks of primary human T cells with base-editing screens.
    Article Snippet: Single-nucleotide variants (SNVs) in key T cell genes can drive clinical pathologies and could be repurposed to improve cellular cancer immunotherapies.. Here, we perform massively parallel base-editing screens to generate thousands of variants at gene loci annotated with known or potential clinical relevance.. We discover a broad landscape of putative gain-of-function (GOF) and loss-of-function (LOF) mutations, including in PIK3CD and the gene encoding its regulatory subunit, PIK3R1, LCK, SOS1, AKT1 and RHOA.

    Article Title: Heat Stress Triggers Nuclear Invagination and Spatial Compartmentalization of Protein Metabolism.
    Article Snippet: .. For proximity labeling experiments, VIM and NES gene fragments were PCR- amplified from cDNA templates, whereas the APEX2 gene fragment was PCR- amplified from a template plasmid (Addgene #124617). .. These fragments were inserted into the plx304 backbone (Addgene #124617) using seamless cloning.

    Article Title: Transposable element-gene chimera cartography, origination and role in enhancing transcriptome plasticity.
    Article Snippet: For sequencing, RNA libraries were prepared using the NEBNext Ultra II directional RNA library prep kit for Illumina (New England Biolabs) following the manufacturer’s recommendations. .. cDNA for the flag-tagged Dux gene (Gene ID 664783), either with or without the transactivation domain (amino acids 1–178), was PCR-amplified from the template plasmid (Addgene, 138320). ..

    Article Title: Auto-methylation of the histone methyltransferase SetDB1 at its histone-mimic motifs ensures the spreading and maintenance of heterochromatin
    Article Snippet: .. The EGFP fragment with intron was synthesized by gBlocks, the other elements of the reporter were amplified from a template plasmid (Addgene 78099). .. Integration of the reporter was performed by co-transfecting 200 ng of reporter plasmid and 1.8 μg of pOG44 plasmid (Invitrogen, V600520) into the Flp-In-HEK293 cells using Lipofectamine 3000 (Invitrogen, L3000008). pOG44 expresses a modified Flp recombinase, which mediates site specific integration of the reporter to the FRT site.

    Cloning:

    Article Title: Supporting Information for Linear motif specificity in signaling through p38α and ERK2 mitogen- activated protein kinases
    Article Snippet: .. The mammalian expression construct for mouse MLXIPL was generated by PCR-amplification from a template plasmid (from the laboratory of Isabelle Leclerc, Addgene #39235) and cloning into pcDNA3 with an N-terminal FLAG epitope tag by Gibson assembly. .. The mammalian expression vector for 3xFLAG-tagged human MGA produced by the laboratory of Guntram Suske was purchased from Addgene (#107715) and modified by overlap extension PCR to remove residues 1052-3065.

    FLAG-tag:

    Article Title: Supporting Information for Linear motif specificity in signaling through p38α and ERK2 mitogen- activated protein kinases
    Article Snippet: .. The mammalian expression construct for mouse MLXIPL was generated by PCR-amplification from a template plasmid (from the laboratory of Isabelle Leclerc, Addgene #39235) and cloning into pcDNA3 with an N-terminal FLAG epitope tag by Gibson assembly. .. The mammalian expression vector for 3xFLAG-tagged human MGA produced by the laboratory of Guntram Suske was purchased from Addgene (#107715) and modified by overlap extension PCR to remove residues 1052-3065.

    Synthesized:

    Article Title: Auto-methylation of the histone methyltransferase SetDB1 at its histone-mimic motifs ensures the spreading and maintenance of heterochromatin
    Article Snippet: .. HP1α or SetDB1 fragments were synthesized by Twist bioscience, pEF1α was amplified from a template plasmid (Addgene, 78099), BGHpA was amplified from the pcDNA5/FRT/TO vector, and the puromycin resistance gene was amplified from the PiggyBac vector backbone. ..

    Article Title: Auto-methylation of the histone methyltransferase SetDB1 at its histone-mimic motifs ensures the spreading and maintenance of heterochromatin
    Article Snippet: .. The EGFP fragment with intron was synthesized by gBlocks, the other elements of the reporter were amplified from a template plasmid (Addgene 78099). .. Integration of the reporter was performed by co-transfecting 200 ng of reporter plasmid and 1.8 μg of pOG44 plasmid (Invitrogen, V600520) into the Flp-In-HEK293 cells using Lipofectamine 3000 (Invitrogen, L3000008). pOG44 expresses a modified Flp recombinase, which mediates site specific integration of the reporter to the FRT site.

    Amplification:

    Article Title: Auto-methylation of the histone methyltransferase SetDB1 at its histone-mimic motifs ensures the spreading and maintenance of heterochromatin
    Article Snippet: .. HP1α or SetDB1 fragments were synthesized by Twist bioscience, pEF1α was amplified from a template plasmid (Addgene, 78099), BGHpA was amplified from the pcDNA5/FRT/TO vector, and the puromycin resistance gene was amplified from the PiggyBac vector backbone. ..

    Article Title: Non-uniform temporal scaling of neurogenesis for species-specific dosing of cortical excitatory subtypes
    Article Snippet: The coding sequences of rat (NM_171992.6) and human (NM_053056.3) CCND1 were synthesized and subcloned into a CAG promoter-driven expression plasmid (pCAG-IRES-GFP)( ) (GenScript Inc.). .. Membrane-anchored Achilles was amplified by PCR using primers with a membrane targeting signal sequence from the template plasmid (Addgene #153528) and subcloned into a pCAG plasmid. siRNAs for Ccnd1 knockdown were purchased from QIAGEN: Negative control; Cat# 1027310, rat Ccnd1; Cat# SI04717881 and Cat# SI01496222. ..

    Article Title: Heat Stress Triggers Nuclear Invagination and Spatial Compartmentalization of Protein Metabolism.
    Article Snippet: .. For proximity labeling experiments, VIM and NES gene fragments were PCR- amplified from cDNA templates, whereas the APEX2 gene fragment was PCR- amplified from a template plasmid (Addgene #124617). .. These fragments were inserted into the plx304 backbone (Addgene #124617) using seamless cloning.

    Article Title: Auto-methylation of the histone methyltransferase SetDB1 at its histone-mimic motifs ensures the spreading and maintenance of heterochromatin
    Article Snippet: .. The EGFP fragment with intron was synthesized by gBlocks, the other elements of the reporter were amplified from a template plasmid (Addgene 78099). .. Integration of the reporter was performed by co-transfecting 200 ng of reporter plasmid and 1.8 μg of pOG44 plasmid (Invitrogen, V600520) into the Flp-In-HEK293 cells using Lipofectamine 3000 (Invitrogen, L3000008). pOG44 expresses a modified Flp recombinase, which mediates site specific integration of the reporter to the FRT site.

    Membrane:

    Article Title: Non-uniform temporal scaling of neurogenesis for species-specific dosing of cortical excitatory subtypes
    Article Snippet: The coding sequences of rat (NM_171992.6) and human (NM_053056.3) CCND1 were synthesized and subcloned into a CAG promoter-driven expression plasmid (pCAG-IRES-GFP)( ) (GenScript Inc.). .. Membrane-anchored Achilles was amplified by PCR using primers with a membrane targeting signal sequence from the template plasmid (Addgene #153528) and subcloned into a pCAG plasmid. siRNAs for Ccnd1 knockdown were purchased from QIAGEN: Negative control; Cat# 1027310, rat Ccnd1; Cat# SI04717881 and Cat# SI01496222. ..

    Sequencing:

    Article Title: Non-uniform temporal scaling of neurogenesis for species-specific dosing of cortical excitatory subtypes
    Article Snippet: The coding sequences of rat (NM_171992.6) and human (NM_053056.3) CCND1 were synthesized and subcloned into a CAG promoter-driven expression plasmid (pCAG-IRES-GFP)( ) (GenScript Inc.). .. Membrane-anchored Achilles was amplified by PCR using primers with a membrane targeting signal sequence from the template plasmid (Addgene #153528) and subcloned into a pCAG plasmid. siRNAs for Ccnd1 knockdown were purchased from QIAGEN: Negative control; Cat# 1027310, rat Ccnd1; Cat# SI04717881 and Cat# SI01496222. ..

    Knockdown:

    Article Title: Non-uniform temporal scaling of neurogenesis for species-specific dosing of cortical excitatory subtypes
    Article Snippet: The coding sequences of rat (NM_171992.6) and human (NM_053056.3) CCND1 were synthesized and subcloned into a CAG promoter-driven expression plasmid (pCAG-IRES-GFP)( ) (GenScript Inc.). .. Membrane-anchored Achilles was amplified by PCR using primers with a membrane targeting signal sequence from the template plasmid (Addgene #153528) and subcloned into a pCAG plasmid. siRNAs for Ccnd1 knockdown were purchased from QIAGEN: Negative control; Cat# 1027310, rat Ccnd1; Cat# SI04717881 and Cat# SI01496222. ..

    Negative Control:

    Article Title: Non-uniform temporal scaling of neurogenesis for species-specific dosing of cortical excitatory subtypes
    Article Snippet: The coding sequences of rat (NM_171992.6) and human (NM_053056.3) CCND1 were synthesized and subcloned into a CAG promoter-driven expression plasmid (pCAG-IRES-GFP)( ) (GenScript Inc.). .. Membrane-anchored Achilles was amplified by PCR using primers with a membrane targeting signal sequence from the template plasmid (Addgene #153528) and subcloned into a pCAG plasmid. siRNAs for Ccnd1 knockdown were purchased from QIAGEN: Negative control; Cat# 1027310, rat Ccnd1; Cat# SI04717881 and Cat# SI01496222. ..

    Labeling:

    Article Title: Heat Stress Triggers Nuclear Invagination and Spatial Compartmentalization of Protein Metabolism.
    Article Snippet: .. For proximity labeling experiments, VIM and NES gene fragments were PCR- amplified from cDNA templates, whereas the APEX2 gene fragment was PCR- amplified from a template plasmid (Addgene #124617). .. These fragments were inserted into the plx304 backbone (Addgene #124617) using seamless cloning.



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    Image Search Results


    The trajectories of OSNs in slit1a, slit1b, slit2 , or slit3 knockdown embryos were compared to uninjected sibling controls at 72hpf. (A, B) Neither slit2 or slit3 knockdown induced errors in DZ-projecting BacOR130-1 or CZ-projecting BacOR111-7 OSNs. (C) Neither slit1a or slit1b knockdown induced errors in CZ-projecting BacOR111-7 OSNs. (D, E, F) Similar results were obtained when both slit2 and slit3 were knocked down together or when both slit1a and slit1b were knocked down together. Statistical comparisons were performed using a two-tailed Fisher’s Exact test.

    Journal: bioRxiv

    Article Title: The contribution of Robos to olfactory sensory axon targeting in the olfactory bulb

    doi: 10.64898/2026.03.19.709608

    Figure Lengend Snippet: The trajectories of OSNs in slit1a, slit1b, slit2 , or slit3 knockdown embryos were compared to uninjected sibling controls at 72hpf. (A, B) Neither slit2 or slit3 knockdown induced errors in DZ-projecting BacOR130-1 or CZ-projecting BacOR111-7 OSNs. (C) Neither slit1a or slit1b knockdown induced errors in CZ-projecting BacOR111-7 OSNs. (D, E, F) Similar results were obtained when both slit2 and slit3 were knocked down together or when both slit1a and slit1b were knocked down together. Statistical comparisons were performed using a two-tailed Fisher’s Exact test.

    Article Snippet: In situ probe templates for slit1a (Addgene plasmid # 61279; http://n2t.net/addgene:61279 ; RRID:Addgene_61279) and slit1b (Addgene plasmid # 61280; http://n2t.net/addgene:61280 ; RRID:Addgene_61280) were a generous gift from Chi–Bin Chien & Lara Hutson ( ).

    Techniques: Knockdown, Two Tailed Test

    Fluorescent in situ hybridization was performed at 48 hpf in slit2 F0 knockdown embryos. Hybridization was performed in parallel at the same time as the preparations presented in . Representative 3-µm optical maximum-intensity projections from individual preparations are shown. Upregulation of slit1a and slit3 mRNA in slit2 knockdowns was observed in 3 independent experiments.

    Journal: bioRxiv

    Article Title: The contribution of Robos to olfactory sensory axon targeting in the olfactory bulb

    doi: 10.64898/2026.03.19.709608

    Figure Lengend Snippet: Fluorescent in situ hybridization was performed at 48 hpf in slit2 F0 knockdown embryos. Hybridization was performed in parallel at the same time as the preparations presented in . Representative 3-µm optical maximum-intensity projections from individual preparations are shown. Upregulation of slit1a and slit3 mRNA in slit2 knockdowns was observed in 3 independent experiments.

    Article Snippet: In situ probe templates for slit1a (Addgene plasmid # 61279; http://n2t.net/addgene:61279 ; RRID:Addgene_61279) and slit1b (Addgene plasmid # 61280; http://n2t.net/addgene:61280 ; RRID:Addgene_61280) were a generous gift from Chi–Bin Chien & Lara Hutson ( ).

    Techniques: In Situ Hybridization, Knockdown, Hybridization