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The trajectories of OSNs in <t>slit1a,</t> slit1b, slit2 , or slit3 knockdown embryos were compared to uninjected sibling controls at 72hpf. (A, B) Neither slit2 or slit3 knockdown induced errors in DZ-projecting BacOR130-1 or CZ-projecting BacOR111-7 OSNs. (C) Neither slit1a or slit1b knockdown induced errors in CZ-projecting BacOR111-7 OSNs. (D, E, F) Similar results were obtained when both slit2 and slit3 were knocked down together or when both slit1a and slit1b were knocked down together. Statistical comparisons were performed using a two-tailed Fisher’s Exact test.
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Image Search Results


The trajectories of OSNs in slit1a, slit1b, slit2 , or slit3 knockdown embryos were compared to uninjected sibling controls at 72hpf. (A, B) Neither slit2 or slit3 knockdown induced errors in DZ-projecting BacOR130-1 or CZ-projecting BacOR111-7 OSNs. (C) Neither slit1a or slit1b knockdown induced errors in CZ-projecting BacOR111-7 OSNs. (D, E, F) Similar results were obtained when both slit2 and slit3 were knocked down together or when both slit1a and slit1b were knocked down together. Statistical comparisons were performed using a two-tailed Fisher’s Exact test.

Journal: bioRxiv

Article Title: The contribution of Robos to olfactory sensory axon targeting in the olfactory bulb

doi: 10.64898/2026.03.19.709608

Figure Lengend Snippet: The trajectories of OSNs in slit1a, slit1b, slit2 , or slit3 knockdown embryos were compared to uninjected sibling controls at 72hpf. (A, B) Neither slit2 or slit3 knockdown induced errors in DZ-projecting BacOR130-1 or CZ-projecting BacOR111-7 OSNs. (C) Neither slit1a or slit1b knockdown induced errors in CZ-projecting BacOR111-7 OSNs. (D, E, F) Similar results were obtained when both slit2 and slit3 were knocked down together or when both slit1a and slit1b were knocked down together. Statistical comparisons were performed using a two-tailed Fisher’s Exact test.

Article Snippet: In situ probe templates for slit1a (Addgene plasmid # 61279; http://n2t.net/addgene:61279 ; RRID:Addgene_61279) and slit1b (Addgene plasmid # 61280; http://n2t.net/addgene:61280 ; RRID:Addgene_61280) were a generous gift from Chi–Bin Chien & Lara Hutson ( ).

Techniques: Knockdown, Two Tailed Test

Fluorescent in situ hybridization was performed at 48 hpf in slit2 F0 knockdown embryos. Hybridization was performed in parallel at the same time as the preparations presented in . Representative 3-µm optical maximum-intensity projections from individual preparations are shown. Upregulation of slit1a and slit3 mRNA in slit2 knockdowns was observed in 3 independent experiments.

Journal: bioRxiv

Article Title: The contribution of Robos to olfactory sensory axon targeting in the olfactory bulb

doi: 10.64898/2026.03.19.709608

Figure Lengend Snippet: Fluorescent in situ hybridization was performed at 48 hpf in slit2 F0 knockdown embryos. Hybridization was performed in parallel at the same time as the preparations presented in . Representative 3-µm optical maximum-intensity projections from individual preparations are shown. Upregulation of slit1a and slit3 mRNA in slit2 knockdowns was observed in 3 independent experiments.

Article Snippet: In situ probe templates for slit1a (Addgene plasmid # 61279; http://n2t.net/addgene:61279 ; RRID:Addgene_61279) and slit1b (Addgene plasmid # 61280; http://n2t.net/addgene:61280 ; RRID:Addgene_61280) were a generous gift from Chi–Bin Chien & Lara Hutson ( ).

Techniques: In Situ Hybridization, Knockdown, Hybridization