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Structured Review

Nacalai temed
Temed, supplied by Nacalai, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/temed/temed/pm42106316-454-18-19
Average 86 stars, based on 1 article reviews
temed - by Bioz Stars, 2026-09
86/100 stars

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Related Articles

Single-cell Analysis:

Article Title: ZO-1 shuttles between apical junctional complexes and podosomes by riding ERK activation waves.
Article Snippet: .. For single cell analysis, the gel solution was prepared with 10% acrylamide, 0.25% bisacrylamide, 0.8% ammonium persulfate, 0.08% TEMED (Nacalai Tesque), and 0.1% dark-red fluorescent carboxylate-modified beads (0.2 μm diameter; Thermo Fisher Scientific, F8807). .. 13 μL of the mixture was placed on a 35 mm glass-based dish (IWAKI, glass 27 mm ø) and covered with a glass coverslip (Matsunami, 15 mm ø).

SDS Page:

Article Title: Vitronectin defines a differentiation-restraining extracellular matrix state in myogenic cells
Article Snippet: Protein concentration was determined using the Qubit Protein Assay Kit (Invitrogen, Q33211). .. Proteins were separated by SDS-PAGE using a 12% separating gel and a 4.5% stacking gel prepared from 30% (w/v) acrylamide/bis-acrylamide solution (29:1; FUJIFILM Wako, 015-25635), 1 M Tris-HCl (pH 8.8 for the separating gel, pH 6.8 for the stacking gel), 10% SDS, 10% (w/v) ammonium persulfate (APS; nacalai tesque, 02634-34), and TEMED (nacalai tesque, 33401-72). .. Electrophoresis was performed in 1× SDS running buffer, and proteins were transferred onto a PVDF membrane (MERCK, IPVH0010) using the HorizeBLOT 2M system (ATTO, WSE-4025) at 18 V/153 mA for 30 min. Membranes were blocked for 1h at room temperature with either 0.3% skim milk in TBST (TBS + 0.1% Tween-20 (FUJIFILM Wako, 166-21213) or EzBlock Chemi (ATTO, AE-1475).

Article Title: AP-1B regulates interactions of epithelial cells and intraepithelial lymphocytes in the intestine
Article Snippet: After centrifuge, samples were slowly taken from the rotor, and dispensed the fractionated samples into protein low bind tubes (Eppendorf, Hamburg, Germany). .. Separating gel for SDS-PAGE were made from, ×4 separating gel buffer solution, acrylamide/bis solution, TEMED, APS (all from Nacalai Tesque) and distilled water. .. Stacking gel was made from, ×4 stacking gel buffer solution, acrylamide/bis solution, TEMED, APS (all from Nacalai Tesque) and distilled water.

Article Title: AP-1B regulates interactions of epithelial cells and intraepithelial lymphocytes in the intestine.
Article Snippet: After centrifuge, samples were slowly taken from the rotor, and dispensed the fractionated samples into protein low bind tubes (Eppendorf, Hamburg, Germany). .. Separating gel for SDS-PAGE were made from, ×4 separating gel buffer solution, acrylamide/bis solution, TEMED, APS (all from Nacalai Tesque) and distilled water. .. Stacking gel was made from, ×4 stacking gel buffer solution, acrylamide/bis solution, TEMED, APS (all from Nacalai Tesque) and distilled water.



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