temed (Euromedex)
86
Structured Review
Euromedex
temed
Temed, supplied by Euromedex, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/temed/temed/pm42107878-87-39-41
Average 86 stars, based on 1 article reviews
Temed, supplied by Euromedex, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/temed/temed/pm42107878-87-39-41
Average 86 stars, based on 1 article reviews
temed - by Bioz Stars,
2026-10
86/100 stars
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Incubation:Article Title: TFK1, a basal body transition fibre protein that is essential for cytokinesis in Trypanosoma brucei. Article Snippet: .. For the gelation step, coverslips were gently deposited on top of a 35 μl drop of MS solution [23% sodium acrylate (AK scientific R624), 10% acrylamide (Euromedex EU0060-A), 0.1% bisacrylamide (Euromedex EU0560-A) in PBS] for 2 min then transferred to a 35 μl drop of MS solution with 0.5% Article Title: Structural and functional studies of the first tripartite protein complex at the Trypanosoma brucei flagellar pocket collar Article Snippet: .. For the gelation step, coverslips were gently deposited on top of a 35 μl drop of MS solution (23% Sodium acrylate (Sigma 408220); 10% Acrylamide (Euromedex EU0060-A); 0.1% Bis-acrylamide (Euromedex EU0560-A) in PBS) for 2 min then transferred to a 35 μl drop of MS; 0.5% Reverse Transcription:Article Title: Monitored eCLIP: high accuracy mapping of RNA-protein interactions Article Snippet: .. Reverse Transcription SuperScript IV Reverse Transcriptase (Invitrogen, 18090050) Exonuclease I (New England Biolabs, M0293S) PCR Amplification qPCR device: CFX-96 Real-Time PCR detection system (BioRad) SYBR Select Master Mix (Applied Biosystems, 4472908) Q5 High-Fidelity DNA Polymerase (New England Biolabs, M0491L) SYBR- Gold nucleic acid gel stain (Life Technologies, S11494) Size selection Agencourt AMPure XP (Beckman Coulter, A63880) Homemade 6% native 1X TBE PAGE gels made with: • 40% acrylamide stock (19/1 acrylamide / bisacrylamide) (Euromedex, EU0076-C) • 10 X TBE buffer (for 1L : Tris base: 108 g ;; boric acid: 55 g ;; EDTA: 7,44 g ;; pH adjusted at 8.3 with HCl) • Ammonium persulfate (Euromedex, EU0009-B) Polymerase Chain Reaction:Article Title: Monitored eCLIP: high accuracy mapping of RNA-protein interactions Article Snippet: .. Reverse Transcription SuperScript IV Reverse Transcriptase (Invitrogen, 18090050) Exonuclease I (New England Biolabs, M0293S) PCR Amplification qPCR device: CFX-96 Real-Time PCR detection system (BioRad) SYBR Select Master Mix (Applied Biosystems, 4472908) Q5 High-Fidelity DNA Polymerase (New England Biolabs, M0491L) SYBR- Gold nucleic acid gel stain (Life Technologies, S11494) Size selection Agencourt AMPure XP (Beckman Coulter, A63880) Homemade 6% native 1X TBE PAGE gels made with: • 40% acrylamide stock (19/1 acrylamide / bisacrylamide) (Euromedex, EU0076-C) • 10 X TBE buffer (for 1L : Tris base: 108 g ;; boric acid: 55 g ;; EDTA: 7,44 g ;; pH adjusted at 8.3 with HCl) • Ammonium persulfate (Euromedex, EU0009-B) Amplification:Article Title: Monitored eCLIP: high accuracy mapping of RNA-protein interactions Article Snippet: .. Reverse Transcription SuperScript IV Reverse Transcriptase (Invitrogen, 18090050) Exonuclease I (New England Biolabs, M0293S) PCR Amplification qPCR device: CFX-96 Real-Time PCR detection system (BioRad) SYBR Select Master Mix (Applied Biosystems, 4472908) Q5 High-Fidelity DNA Polymerase (New England Biolabs, M0491L) SYBR- Gold nucleic acid gel stain (Life Technologies, S11494) Size selection Agencourt AMPure XP (Beckman Coulter, A63880) Homemade 6% native 1X TBE PAGE gels made with: • 40% acrylamide stock (19/1 acrylamide / bisacrylamide) (Euromedex, EU0076-C) • 10 X TBE buffer (for 1L : Tris base: 108 g ;; boric acid: 55 g ;; EDTA: 7,44 g ;; pH adjusted at 8.3 with HCl) • Ammonium persulfate (Euromedex, EU0009-B) Real-time Polymerase Chain Reaction:Article Title: Monitored eCLIP: high accuracy mapping of RNA-protein interactions Article Snippet: .. Reverse Transcription SuperScript IV Reverse Transcriptase (Invitrogen, 18090050) Exonuclease I (New England Biolabs, M0293S) PCR Amplification qPCR device: CFX-96 Real-Time PCR detection system (BioRad) SYBR Select Master Mix (Applied Biosystems, 4472908) Q5 High-Fidelity DNA Polymerase (New England Biolabs, M0491L) SYBR- Gold nucleic acid gel stain (Life Technologies, S11494) Size selection Agencourt AMPure XP (Beckman Coulter, A63880) Homemade 6% native 1X TBE PAGE gels made with: • 40% acrylamide stock (19/1 acrylamide / bisacrylamide) (Euromedex, EU0076-C) • 10 X TBE buffer (for 1L : Tris base: 108 g ;; boric acid: 55 g ;; EDTA: 7,44 g ;; pH adjusted at 8.3 with HCl) • Ammonium persulfate (Euromedex, EU0009-B) Staining:Article Title: Monitored eCLIP: high accuracy mapping of RNA-protein interactions Article Snippet: .. Reverse Transcription SuperScript IV Reverse Transcriptase (Invitrogen, 18090050) Exonuclease I (New England Biolabs, M0293S) PCR Amplification qPCR device: CFX-96 Real-Time PCR detection system (BioRad) SYBR Select Master Mix (Applied Biosystems, 4472908) Q5 High-Fidelity DNA Polymerase (New England Biolabs, M0491L) SYBR- Gold nucleic acid gel stain (Life Technologies, S11494) Size selection Agencourt AMPure XP (Beckman Coulter, A63880) Homemade 6% native 1X TBE PAGE gels made with: • 40% acrylamide stock (19/1 acrylamide / bisacrylamide) (Euromedex, EU0076-C) • 10 X TBE buffer (for 1L : Tris base: 108 g ;; boric acid: 55 g ;; EDTA: 7,44 g ;; pH adjusted at 8.3 with HCl) • Ammonium persulfate (Euromedex, EU0009-B) Article Title: A Conserved 3'UTR Stem-loop Directs UPF1/eIF4AIII-Dependent Regulation of GABARAPL1 mRNA. Article Snippet: .. For acrylamide gel, a mix of 2.1 mL of Acrylamide (Purity 99.4%)//Bis-Acrylamide (Ultrapure) 30% Sol Ratio 29/1 (EU0073, Euromedex), 3.9 mL of 0.5X TAE buffer (Tris-acetate-EDTA 50X), 50 μL of 10% ammonium persulfate (EU0009, Euromedex), and 7 μL of Size Selection:Article Title: Monitored eCLIP: high accuracy mapping of RNA-protein interactions Article Snippet: .. Reverse Transcription SuperScript IV Reverse Transcriptase (Invitrogen, 18090050) Exonuclease I (New England Biolabs, M0293S) PCR Amplification qPCR device: CFX-96 Real-Time PCR detection system (BioRad) SYBR Select Master Mix (Applied Biosystems, 4472908) Q5 High-Fidelity DNA Polymerase (New England Biolabs, M0491L) SYBR- Gold nucleic acid gel stain (Life Technologies, S11494) Size selection Agencourt AMPure XP (Beckman Coulter, A63880) Homemade 6% native 1X TBE PAGE gels made with: • 40% acrylamide stock (19/1 acrylamide / bisacrylamide) (Euromedex, EU0076-C) • 10 X TBE buffer (for 1L : Tris base: 108 g ;; boric acid: 55 g ;; EDTA: 7,44 g ;; pH adjusted at 8.3 with HCl) • Ammonium persulfate (Euromedex, EU0009-B) Polyacrylamide Gel Electrophoresis:Article Title: Monitored eCLIP: high accuracy mapping of RNA-protein interactions Article Snippet: .. Reverse Transcription SuperScript IV Reverse Transcriptase (Invitrogen, 18090050) Exonuclease I (New England Biolabs, M0293S) PCR Amplification qPCR device: CFX-96 Real-Time PCR detection system (BioRad) SYBR Select Master Mix (Applied Biosystems, 4472908) Q5 High-Fidelity DNA Polymerase (New England Biolabs, M0491L) SYBR- Gold nucleic acid gel stain (Life Technologies, S11494) Size selection Agencourt AMPure XP (Beckman Coulter, A63880) Homemade 6% native 1X TBE PAGE gels made with: • 40% acrylamide stock (19/1 acrylamide / bisacrylamide) (Euromedex, EU0076-C) • 10 X TBE buffer (for 1L : Tris base: 108 g ;; boric acid: 55 g ;; EDTA: 7,44 g ;; pH adjusted at 8.3 with HCl) • Ammonium persulfate (Euromedex, EU0009-B) Electrophoresis:Article Title: Monitored eCLIP: high accuracy mapping of RNA-protein interactions Article Snippet: .. Reverse Transcription SuperScript IV Reverse Transcriptase (Invitrogen, 18090050) Exonuclease I (New England Biolabs, M0293S) PCR Amplification qPCR device: CFX-96 Real-Time PCR detection system (BioRad) SYBR Select Master Mix (Applied Biosystems, 4472908) Q5 High-Fidelity DNA Polymerase (New England Biolabs, M0491L) SYBR- Gold nucleic acid gel stain (Life Technologies, S11494) Size selection Agencourt AMPure XP (Beckman Coulter, A63880) Homemade 6% native 1X TBE PAGE gels made with: • 40% acrylamide stock (19/1 acrylamide / bisacrylamide) (Euromedex, EU0076-C) • 10 X TBE buffer (for 1L : Tris base: 108 g ;; boric acid: 55 g ;; EDTA: 7,44 g ;; pH adjusted at 8.3 with HCl) • Ammonium persulfate (Euromedex, EU0009-B) Acrylamide Gel Assay:Article Title: A Conserved 3'UTR Stem-loop Directs UPF1/eIF4AIII-Dependent Regulation of GABARAPL1 mRNA. Article Snippet: .. For acrylamide gel, a mix of 2.1 mL of Acrylamide (Purity 99.4%)//Bis-Acrylamide (Ultrapure) 30% Sol Ratio 29/1 (EU0073, Euromedex), 3.9 mL of 0.5X TAE buffer (Tris-acetate-EDTA 50X), 50 μL of 10% ammonium persulfate (EU0009, Euromedex), and 7 μL of Lysis:Article Title: Random Mutagenesis Using Error-Prone DNA Polymerases. Article Snippet: “Random mutagenesis” is a technique that allows researchers to develop large libraries of variants of a particular DNA sequence.. Once developed, these libraries can then be used for several purposes, including structure–function and directed evolution studies.. Random mutagenesis is different from other mutagenesis techniques in that it does not require the researcher to have any prior knowledge about the structural properties of the DNA sequence being targeted, thus allowing for the unbiased discovery of novel or beneficial mutations. Sterility:Article Title: Random Mutagenesis Using Error-Prone DNA Polymerases. Article Snippet: “Random mutagenesis” is a technique that allows researchers to develop large libraries of variants of a particular DNA sequence.. Once developed, these libraries can then be used for several purposes, including structure–function and directed evolution studies.. Random mutagenesis is different from other mutagenesis techniques in that it does not require the researcher to have any prior knowledge about the structural properties of the DNA sequence being targeted, thus allowing for the unbiased discovery of novel or beneficial mutations. Plasmid Preparation:Article Title: Random Mutagenesis Using Error-Prone DNA Polymerases. Article Snippet: “Random mutagenesis” is a technique that allows researchers to develop large libraries of variants of a particular DNA sequence.. Once developed, these libraries can then be used for several purposes, including structure–function and directed evolution studies.. Random mutagenesis is different from other mutagenesis techniques in that it does not require the researcher to have any prior knowledge about the structural properties of the DNA sequence being targeted, thus allowing for the unbiased discovery of novel or beneficial mutations. Protease Inhibitor:Article Title: Random Mutagenesis Using Error-Prone DNA Polymerases. Article Snippet: “Random mutagenesis” is a technique that allows researchers to develop large libraries of variants of a particular DNA sequence.. Once developed, these libraries can then be used for several purposes, including structure–function and directed evolution studies.. Random mutagenesis is different from other mutagenesis techniques in that it does not require the researcher to have any prior knowledge about the structural properties of the DNA sequence being targeted, thus allowing for the unbiased discovery of novel or beneficial mutations. Nucleic Acid Electrophoresis:Article Title: Random Mutagenesis Using Error-Prone DNA Polymerases. Article Snippet: “Random mutagenesis” is a technique that allows researchers to develop large libraries of variants of a particular DNA sequence.. Once developed, these libraries can then be used for several purposes, including structure–function and directed evolution studies.. Random mutagenesis is different from other mutagenesis techniques in that it does not require the researcher to have any prior knowledge about the structural properties of the DNA sequence being targeted, thus allowing for the unbiased discovery of novel or beneficial mutations. Ligation:Article Title: Random Mutagenesis Using Error-Prone DNA Polymerases. Article Snippet: “Random mutagenesis” is a technique that allows researchers to develop large libraries of variants of a particular DNA sequence.. Once developed, these libraries can then be used for several purposes, including structure–function and directed evolution studies.. Random mutagenesis is different from other mutagenesis techniques in that it does not require the researcher to have any prior knowledge about the structural properties of the DNA sequence being targeted, thus allowing for the unbiased discovery of novel or beneficial mutations. |