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Euromedex temed
Temed, supplied by Euromedex, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/temed/temed/pm42107878-87-39-41
Average 86 stars, based on 1 article reviews
temed - by Bioz Stars, 2026-10
86/100 stars

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Related Articles

Incubation:

Article Title: TFK1, a basal body transition fibre protein that is essential for cytokinesis in Trypanosoma brucei.
Article Snippet: .. For the gelation step, coverslips were gently deposited on top of a 35 μl drop of MS solution [23% sodium acrylate (AK scientific R624), 10% acrylamide (Euromedex EU0060-A), 0.1% bisacrylamide (Euromedex EU0560-A) in PBS] for 2 min then transferred to a 35 μl drop of MS solution with 0.5% TEMED (Euromedex 50406-A) and 0.5% ammonium persulfate (Euromedex EU0009-A) for 5 min on ice then transferred at 37°C and incubated for 1 h in a moist chamber without agitation. .. The coverslips were then transferred to a 6-well plate in 1 ml of denaturation solution (200 mM SDS, 200 mM sodium chloride, 50 mM Tris-HCl pH 9.0) with agitation at RT for 15 min to detach the gel from the coverslip, that was then moved into a 1.5 ml Eppendorf centrifuge tube filled with denaturation solution and incubated at 95°C for one 90 min. Gels were expanded in large volumes of deionized water (twice 30 min then overnight) then incubated in a large volume of PBS for 10 min (three times).

Article Title: Structural and functional studies of the first tripartite protein complex at the Trypanosoma brucei flagellar pocket collar
Article Snippet: .. For the gelation step, coverslips were gently deposited on top of a 35 μl drop of MS solution (23% Sodium acrylate (Sigma 408220); 10% Acrylamide (Euromedex EU0060-A); 0.1% Bis-acrylamide (Euromedex EU0560-A) in PBS) for 2 min then transferred to a 35 μl drop of MS; 0.5% TEMED (Euromedex 50406-A); 0.5% Ammonium persulfate (Euromedex EU0009-A) for 5 minutes on ice then transferred at 37°C and incubated for 1 hour in a moist chamber without agitation. .. The coverslips were then transferred in 6-well plate in 1 mL of denaturation solution (200 mM Sodium Dodecyl Sulfate; 200 mM Sodium chloride; 50 mM Tris pH 9.0) with agitation at RT for 15 min to detach the gel from the coverslip, then moved into a 1.5 ml Eppendorf centrifuge tube filled with denaturation solution and incubated at 95°C for one 90 minutes.

Reverse Transcription:

Article Title: Monitored eCLIP: high accuracy mapping of RNA-protein interactions
Article Snippet: .. Reverse Transcription SuperScript IV Reverse Transcriptase (Invitrogen, 18090050) Exonuclease I (New England Biolabs, M0293S) PCR Amplification qPCR device: CFX-96 Real-Time PCR detection system (BioRad) SYBR Select Master Mix (Applied Biosystems, 4472908) Q5 High-Fidelity DNA Polymerase (New England Biolabs, M0491L) SYBR- Gold nucleic acid gel stain (Life Technologies, S11494) Size selection Agencourt AMPure XP (Beckman Coulter, A63880) Homemade 6% native 1X TBE PAGE gels made with: • 40% acrylamide stock (19/1 acrylamide / bisacrylamide) (Euromedex, EU0076-C) • 10 X TBE buffer (for 1L : Tris base: 108 g ;; boric acid: 55 g ;; EDTA: 7,44 g ;; pH adjusted at 8.3 with HCl) • Ammonium persulfate (Euromedex, EU0009-B) • TEMED (Euromedex, 50406) Mini-PROTEAN Tetra System for gel casting and electrophoresis (BioRad) GeneRuler Low Range DNA Ladder (Thermo Scientific, SM1193) Blue light Dark Reader Transilluminator (Clare Chemical, DR46B) Buffers: 1X TBE buffer TET buffer: 10 mM Tris–HCl, 1 mM EDTA, pH = 8.0, with 0.05% (v/v) Tween® 20 30% PEG–Bead Solution: 30% (w/v) PEG 8000, 1M NaCl, 1X TE (10 mM Tris, 1 mM EDTA) and 0.05% (v/v) Tween 20. ..

Polymerase Chain Reaction:

Article Title: Monitored eCLIP: high accuracy mapping of RNA-protein interactions
Article Snippet: .. Reverse Transcription SuperScript IV Reverse Transcriptase (Invitrogen, 18090050) Exonuclease I (New England Biolabs, M0293S) PCR Amplification qPCR device: CFX-96 Real-Time PCR detection system (BioRad) SYBR Select Master Mix (Applied Biosystems, 4472908) Q5 High-Fidelity DNA Polymerase (New England Biolabs, M0491L) SYBR- Gold nucleic acid gel stain (Life Technologies, S11494) Size selection Agencourt AMPure XP (Beckman Coulter, A63880) Homemade 6% native 1X TBE PAGE gels made with: • 40% acrylamide stock (19/1 acrylamide / bisacrylamide) (Euromedex, EU0076-C) • 10 X TBE buffer (for 1L : Tris base: 108 g ;; boric acid: 55 g ;; EDTA: 7,44 g ;; pH adjusted at 8.3 with HCl) • Ammonium persulfate (Euromedex, EU0009-B) • TEMED (Euromedex, 50406) Mini-PROTEAN Tetra System for gel casting and electrophoresis (BioRad) GeneRuler Low Range DNA Ladder (Thermo Scientific, SM1193) Blue light Dark Reader Transilluminator (Clare Chemical, DR46B) Buffers: 1X TBE buffer TET buffer: 10 mM Tris–HCl, 1 mM EDTA, pH = 8.0, with 0.05% (v/v) Tween® 20 30% PEG–Bead Solution: 30% (w/v) PEG 8000, 1M NaCl, 1X TE (10 mM Tris, 1 mM EDTA) and 0.05% (v/v) Tween 20. ..

Amplification:

Article Title: Monitored eCLIP: high accuracy mapping of RNA-protein interactions
Article Snippet: .. Reverse Transcription SuperScript IV Reverse Transcriptase (Invitrogen, 18090050) Exonuclease I (New England Biolabs, M0293S) PCR Amplification qPCR device: CFX-96 Real-Time PCR detection system (BioRad) SYBR Select Master Mix (Applied Biosystems, 4472908) Q5 High-Fidelity DNA Polymerase (New England Biolabs, M0491L) SYBR- Gold nucleic acid gel stain (Life Technologies, S11494) Size selection Agencourt AMPure XP (Beckman Coulter, A63880) Homemade 6% native 1X TBE PAGE gels made with: • 40% acrylamide stock (19/1 acrylamide / bisacrylamide) (Euromedex, EU0076-C) • 10 X TBE buffer (for 1L : Tris base: 108 g ;; boric acid: 55 g ;; EDTA: 7,44 g ;; pH adjusted at 8.3 with HCl) • Ammonium persulfate (Euromedex, EU0009-B) • TEMED (Euromedex, 50406) Mini-PROTEAN Tetra System for gel casting and electrophoresis (BioRad) GeneRuler Low Range DNA Ladder (Thermo Scientific, SM1193) Blue light Dark Reader Transilluminator (Clare Chemical, DR46B) Buffers: 1X TBE buffer TET buffer: 10 mM Tris–HCl, 1 mM EDTA, pH = 8.0, with 0.05% (v/v) Tween® 20 30% PEG–Bead Solution: 30% (w/v) PEG 8000, 1M NaCl, 1X TE (10 mM Tris, 1 mM EDTA) and 0.05% (v/v) Tween 20. ..

Real-time Polymerase Chain Reaction:

Article Title: Monitored eCLIP: high accuracy mapping of RNA-protein interactions
Article Snippet: .. Reverse Transcription SuperScript IV Reverse Transcriptase (Invitrogen, 18090050) Exonuclease I (New England Biolabs, M0293S) PCR Amplification qPCR device: CFX-96 Real-Time PCR detection system (BioRad) SYBR Select Master Mix (Applied Biosystems, 4472908) Q5 High-Fidelity DNA Polymerase (New England Biolabs, M0491L) SYBR- Gold nucleic acid gel stain (Life Technologies, S11494) Size selection Agencourt AMPure XP (Beckman Coulter, A63880) Homemade 6% native 1X TBE PAGE gels made with: • 40% acrylamide stock (19/1 acrylamide / bisacrylamide) (Euromedex, EU0076-C) • 10 X TBE buffer (for 1L : Tris base: 108 g ;; boric acid: 55 g ;; EDTA: 7,44 g ;; pH adjusted at 8.3 with HCl) • Ammonium persulfate (Euromedex, EU0009-B) • TEMED (Euromedex, 50406) Mini-PROTEAN Tetra System for gel casting and electrophoresis (BioRad) GeneRuler Low Range DNA Ladder (Thermo Scientific, SM1193) Blue light Dark Reader Transilluminator (Clare Chemical, DR46B) Buffers: 1X TBE buffer TET buffer: 10 mM Tris–HCl, 1 mM EDTA, pH = 8.0, with 0.05% (v/v) Tween® 20 30% PEG–Bead Solution: 30% (w/v) PEG 8000, 1M NaCl, 1X TE (10 mM Tris, 1 mM EDTA) and 0.05% (v/v) Tween 20. ..

Staining:

Article Title: Monitored eCLIP: high accuracy mapping of RNA-protein interactions
Article Snippet: .. Reverse Transcription SuperScript IV Reverse Transcriptase (Invitrogen, 18090050) Exonuclease I (New England Biolabs, M0293S) PCR Amplification qPCR device: CFX-96 Real-Time PCR detection system (BioRad) SYBR Select Master Mix (Applied Biosystems, 4472908) Q5 High-Fidelity DNA Polymerase (New England Biolabs, M0491L) SYBR- Gold nucleic acid gel stain (Life Technologies, S11494) Size selection Agencourt AMPure XP (Beckman Coulter, A63880) Homemade 6% native 1X TBE PAGE gels made with: • 40% acrylamide stock (19/1 acrylamide / bisacrylamide) (Euromedex, EU0076-C) • 10 X TBE buffer (for 1L : Tris base: 108 g ;; boric acid: 55 g ;; EDTA: 7,44 g ;; pH adjusted at 8.3 with HCl) • Ammonium persulfate (Euromedex, EU0009-B) • TEMED (Euromedex, 50406) Mini-PROTEAN Tetra System for gel casting and electrophoresis (BioRad) GeneRuler Low Range DNA Ladder (Thermo Scientific, SM1193) Blue light Dark Reader Transilluminator (Clare Chemical, DR46B) Buffers: 1X TBE buffer TET buffer: 10 mM Tris–HCl, 1 mM EDTA, pH = 8.0, with 0.05% (v/v) Tween® 20 30% PEG–Bead Solution: 30% (w/v) PEG 8000, 1M NaCl, 1X TE (10 mM Tris, 1 mM EDTA) and 0.05% (v/v) Tween 20. ..

Article Title: A Conserved 3'UTR Stem-loop Directs UPF1/eIF4AIII-Dependent Regulation of GABARAPL1 mRNA.
Article Snippet: .. For acrylamide gel, a mix of 2.1 mL of Acrylamide (Purity 99.4%)//Bis-Acrylamide (Ultrapure) 30% Sol Ratio 29/1 (EU0073, Euromedex), 3.9 mL of 0.5X TAE buffer (Tris-acetate-EDTA 50X), 50 μL of 10% ammonium persulfate (EU0009, Euromedex), and 7 μL of TEMED (50406, Euromedex) and 2 μL of ClearSight DNA Stain (EUH40501, Euromedex). ..

Size Selection:

Article Title: Monitored eCLIP: high accuracy mapping of RNA-protein interactions
Article Snippet: .. Reverse Transcription SuperScript IV Reverse Transcriptase (Invitrogen, 18090050) Exonuclease I (New England Biolabs, M0293S) PCR Amplification qPCR device: CFX-96 Real-Time PCR detection system (BioRad) SYBR Select Master Mix (Applied Biosystems, 4472908) Q5 High-Fidelity DNA Polymerase (New England Biolabs, M0491L) SYBR- Gold nucleic acid gel stain (Life Technologies, S11494) Size selection Agencourt AMPure XP (Beckman Coulter, A63880) Homemade 6% native 1X TBE PAGE gels made with: • 40% acrylamide stock (19/1 acrylamide / bisacrylamide) (Euromedex, EU0076-C) • 10 X TBE buffer (for 1L : Tris base: 108 g ;; boric acid: 55 g ;; EDTA: 7,44 g ;; pH adjusted at 8.3 with HCl) • Ammonium persulfate (Euromedex, EU0009-B) • TEMED (Euromedex, 50406) Mini-PROTEAN Tetra System for gel casting and electrophoresis (BioRad) GeneRuler Low Range DNA Ladder (Thermo Scientific, SM1193) Blue light Dark Reader Transilluminator (Clare Chemical, DR46B) Buffers: 1X TBE buffer TET buffer: 10 mM Tris–HCl, 1 mM EDTA, pH = 8.0, with 0.05% (v/v) Tween® 20 30% PEG–Bead Solution: 30% (w/v) PEG 8000, 1M NaCl, 1X TE (10 mM Tris, 1 mM EDTA) and 0.05% (v/v) Tween 20. ..

Polyacrylamide Gel Electrophoresis:

Article Title: Monitored eCLIP: high accuracy mapping of RNA-protein interactions
Article Snippet: .. Reverse Transcription SuperScript IV Reverse Transcriptase (Invitrogen, 18090050) Exonuclease I (New England Biolabs, M0293S) PCR Amplification qPCR device: CFX-96 Real-Time PCR detection system (BioRad) SYBR Select Master Mix (Applied Biosystems, 4472908) Q5 High-Fidelity DNA Polymerase (New England Biolabs, M0491L) SYBR- Gold nucleic acid gel stain (Life Technologies, S11494) Size selection Agencourt AMPure XP (Beckman Coulter, A63880) Homemade 6% native 1X TBE PAGE gels made with: • 40% acrylamide stock (19/1 acrylamide / bisacrylamide) (Euromedex, EU0076-C) • 10 X TBE buffer (for 1L : Tris base: 108 g ;; boric acid: 55 g ;; EDTA: 7,44 g ;; pH adjusted at 8.3 with HCl) • Ammonium persulfate (Euromedex, EU0009-B) • TEMED (Euromedex, 50406) Mini-PROTEAN Tetra System for gel casting and electrophoresis (BioRad) GeneRuler Low Range DNA Ladder (Thermo Scientific, SM1193) Blue light Dark Reader Transilluminator (Clare Chemical, DR46B) Buffers: 1X TBE buffer TET buffer: 10 mM Tris–HCl, 1 mM EDTA, pH = 8.0, with 0.05% (v/v) Tween® 20 30% PEG–Bead Solution: 30% (w/v) PEG 8000, 1M NaCl, 1X TE (10 mM Tris, 1 mM EDTA) and 0.05% (v/v) Tween 20. ..

Electrophoresis:

Article Title: Monitored eCLIP: high accuracy mapping of RNA-protein interactions
Article Snippet: .. Reverse Transcription SuperScript IV Reverse Transcriptase (Invitrogen, 18090050) Exonuclease I (New England Biolabs, M0293S) PCR Amplification qPCR device: CFX-96 Real-Time PCR detection system (BioRad) SYBR Select Master Mix (Applied Biosystems, 4472908) Q5 High-Fidelity DNA Polymerase (New England Biolabs, M0491L) SYBR- Gold nucleic acid gel stain (Life Technologies, S11494) Size selection Agencourt AMPure XP (Beckman Coulter, A63880) Homemade 6% native 1X TBE PAGE gels made with: • 40% acrylamide stock (19/1 acrylamide / bisacrylamide) (Euromedex, EU0076-C) • 10 X TBE buffer (for 1L : Tris base: 108 g ;; boric acid: 55 g ;; EDTA: 7,44 g ;; pH adjusted at 8.3 with HCl) • Ammonium persulfate (Euromedex, EU0009-B) • TEMED (Euromedex, 50406) Mini-PROTEAN Tetra System for gel casting and electrophoresis (BioRad) GeneRuler Low Range DNA Ladder (Thermo Scientific, SM1193) Blue light Dark Reader Transilluminator (Clare Chemical, DR46B) Buffers: 1X TBE buffer TET buffer: 10 mM Tris–HCl, 1 mM EDTA, pH = 8.0, with 0.05% (v/v) Tween® 20 30% PEG–Bead Solution: 30% (w/v) PEG 8000, 1M NaCl, 1X TE (10 mM Tris, 1 mM EDTA) and 0.05% (v/v) Tween 20. ..

Acrylamide Gel Assay:

Article Title: A Conserved 3'UTR Stem-loop Directs UPF1/eIF4AIII-Dependent Regulation of GABARAPL1 mRNA.
Article Snippet: .. For acrylamide gel, a mix of 2.1 mL of Acrylamide (Purity 99.4%)//Bis-Acrylamide (Ultrapure) 30% Sol Ratio 29/1 (EU0073, Euromedex), 3.9 mL of 0.5X TAE buffer (Tris-acetate-EDTA 50X), 50 μL of 10% ammonium persulfate (EU0009, Euromedex), and 7 μL of TEMED (50406, Euromedex) and 2 μL of ClearSight DNA Stain (EUH40501, Euromedex). ..

Lysis:

Article Title: Random Mutagenesis Using Error-Prone DNA Polymerases.
Article Snippet: “Random mutagenesis” is a technique that allows researchers to develop large libraries of variants of a particular DNA sequence.. Once developed, these libraries can then be used for several purposes, including structure–function and directed evolution studies.. Random mutagenesis is different from other mutagenesis techniques in that it does not require the researcher to have any prior knowledge about the structural properties of the DNA sequence being targeted, thus allowing for the unbiased discovery of novel or beneficial mutations.

Sterility:

Article Title: Random Mutagenesis Using Error-Prone DNA Polymerases.
Article Snippet: “Random mutagenesis” is a technique that allows researchers to develop large libraries of variants of a particular DNA sequence.. Once developed, these libraries can then be used for several purposes, including structure–function and directed evolution studies.. Random mutagenesis is different from other mutagenesis techniques in that it does not require the researcher to have any prior knowledge about the structural properties of the DNA sequence being targeted, thus allowing for the unbiased discovery of novel or beneficial mutations.

Plasmid Preparation:

Article Title: Random Mutagenesis Using Error-Prone DNA Polymerases.
Article Snippet: “Random mutagenesis” is a technique that allows researchers to develop large libraries of variants of a particular DNA sequence.. Once developed, these libraries can then be used for several purposes, including structure–function and directed evolution studies.. Random mutagenesis is different from other mutagenesis techniques in that it does not require the researcher to have any prior knowledge about the structural properties of the DNA sequence being targeted, thus allowing for the unbiased discovery of novel or beneficial mutations.

Protease Inhibitor:

Article Title: Random Mutagenesis Using Error-Prone DNA Polymerases.
Article Snippet: “Random mutagenesis” is a technique that allows researchers to develop large libraries of variants of a particular DNA sequence.. Once developed, these libraries can then be used for several purposes, including structure–function and directed evolution studies.. Random mutagenesis is different from other mutagenesis techniques in that it does not require the researcher to have any prior knowledge about the structural properties of the DNA sequence being targeted, thus allowing for the unbiased discovery of novel or beneficial mutations.

Nucleic Acid Electrophoresis:

Article Title: Random Mutagenesis Using Error-Prone DNA Polymerases.
Article Snippet: “Random mutagenesis” is a technique that allows researchers to develop large libraries of variants of a particular DNA sequence.. Once developed, these libraries can then be used for several purposes, including structure–function and directed evolution studies.. Random mutagenesis is different from other mutagenesis techniques in that it does not require the researcher to have any prior knowledge about the structural properties of the DNA sequence being targeted, thus allowing for the unbiased discovery of novel or beneficial mutations.

Ligation:

Article Title: Random Mutagenesis Using Error-Prone DNA Polymerases.
Article Snippet: “Random mutagenesis” is a technique that allows researchers to develop large libraries of variants of a particular DNA sequence.. Once developed, these libraries can then be used for several purposes, including structure–function and directed evolution studies.. Random mutagenesis is different from other mutagenesis techniques in that it does not require the researcher to have any prior knowledge about the structural properties of the DNA sequence being targeted, thus allowing for the unbiased discovery of novel or beneficial mutations.



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