anti tcf4 monoclonal antibody  (Proteintech)

Journal: Cancer Research

Article Title: A Double-Negative Prostate Cancer Subtype Is Vulnerable to SWI/SNF-Targeting Degrader Molecules

doi: 10.1158/0008-5472.CAN-25-2928

Figure Lengend Snippet: TCF7L2 is a dependency in CRPC-WNT. A, Growth data measured by live-cell imaging (IncuCyte S3) upon transfection of indicated siRNA or plasmid (EV or rescue). Immunoblot of indicated proteins at 72 hours after transfection. GAPDH served as loading control. Data are presented as mean values ± SEM and analyzed using two-way ANOVA. ***, P < 0.001. Data are representative of n = 2 independent experiments. B, Spheroid formation measured by live-cell imaging (IncuCyte SX5) upon transfection of indicated siRNA. Data are presented as mean values ± SEM and analyzed using two-way ANOVA. *, P < 0.05; **, P < 0.01; ***, P < 0.001. Data are representative of n = 2 independent experiments. C, IHC and staining intensity of TCF7L2 on indicated organoids upon treatment with 1 µmol/L A858 or 1 µmol/L A947 for 24 hours. Violin plot from TCF7L2 staining intensity analyzed using two-way ANOVA. ****, P < 0.0001. Scale bars, 100 µm (MSK-PCa16) and 50 µm (WCM1078). OD, optical density. D, TOPFlash TCF/LEF reporter assay measured after 48 hours upon treatment with indicated drugs in WCM1078 sublines. FOPFlash served as a negative reporter control. Data are presented as mean values ± SEM after normalization to internal Renilla control and analyzed using a paired Student t test. ***, P < 0.001; ****, P < 0.0001. Data are representative of n = 2 independent experiments.

Article Snippet: The TCF7L2 antibody (Cell Signaling Technology, cat #2569, RRID: AB_2199816) was used for staining.

Techniques: Live Cell Imaging, Transfection, Plasmid Preparation, Western Blot, Control, Staining, Reporter Assay

Journal: Cancer Research

Article Title: A Double-Negative Prostate Cancer Subtype Is Vulnerable to SWI/SNF-Targeting Degrader Molecules

doi: 10.1158/0008-5472.CAN-25-2928

Figure Lengend Snippet: TCF7L2 regulates proproliferative signatures in CRPC-WNT. A, ChIP-seq read density tornado plots from WCM1078 organoids treated with 1 µmol/L A858 or 1 µmol/L A947 for 4 hours ( n = 2 biological replicates). B, Venn diagram indicating A947 treatment–lost regions from ChIP-seq, ATAC-seq, and RNA-seq data in WCM1078. GSEA was performed from 350 overlapping genes. C, Immunoblot of indicated proteins at indicated time upon treatment with 1 µmol/L A947. GAPDH served as loading control. Data are representative of n = 2 independent experiments. D, Dose–response curves with indicated drugs after measurement of proliferation with CellTiter-Glo 2.0 after 7-day treatment ( n = 2 independent experiments).

Article Snippet: The TCF7L2 antibody (Cell Signaling Technology, cat #2569, RRID: AB_2199816) was used for staining.

Techniques: ChIP-sequencing, RNA Sequencing, Western Blot, Control

Journal: Cancer Research

Article Title: A Double-Negative Prostate Cancer Subtype Is Vulnerable to SWI/SNF-Targeting Degrader Molecules

doi: 10.1158/0008-5472.CAN-25-2928

Figure Lengend Snippet: TCF7L2 is a dependency in CRPC-WNT. A, Growth data measured by live-cell imaging (IncuCyte S3) upon transfection of indicated siRNA or plasmid (EV or rescue). Immunoblot of indicated proteins at 72 hours after transfection. GAPDH served as loading control. Data are presented as mean values ± SEM and analyzed using two-way ANOVA. ***, P < 0.001. Data are representative of n = 2 independent experiments. B, Spheroid formation measured by live-cell imaging (IncuCyte SX5) upon transfection of indicated siRNA. Data are presented as mean values ± SEM and analyzed using two-way ANOVA. *, P < 0.05; **, P < 0.01; ***, P < 0.001. Data are representative of n = 2 independent experiments. C, IHC and staining intensity of TCF7L2 on indicated organoids upon treatment with 1 µmol/L A858 or 1 µmol/L A947 for 24 hours. Violin plot from TCF7L2 staining intensity analyzed using two-way ANOVA. ****, P < 0.0001. Scale bars, 100 µm (MSK-PCa16) and 50 µm (WCM1078). OD, optical density. D, TOPFlash TCF/LEF reporter assay measured after 48 hours upon treatment with indicated drugs in WCM1078 sublines. FOPFlash served as a negative reporter control. Data are presented as mean values ± SEM after normalization to internal Renilla control and analyzed using a paired Student t test. ***, P < 0.001; ****, P < 0.0001. Data are representative of n = 2 independent experiments.

Article Snippet: Chromatin was prepared from two biological replicates of WCM1078 treated with A858 or A947 (1 μmol/L) for 4 hours, and chromatin immunoprecipitation sequencing (ChIP-seq) assays were then performed using an antibody against TCF7L2 (Cell Signaling Technology, cat #2569, RRID: AB_2199816).

Techniques: Live Cell Imaging, Transfection, Plasmid Preparation, Western Blot, Control, Staining, Reporter Assay

Journal: Cancer Research

Article Title: A Double-Negative Prostate Cancer Subtype Is Vulnerable to SWI/SNF-Targeting Degrader Molecules

doi: 10.1158/0008-5472.CAN-25-2928

Figure Lengend Snippet: TCF7L2 regulates proproliferative signatures in CRPC-WNT. A, ChIP-seq read density tornado plots from WCM1078 organoids treated with 1 µmol/L A858 or 1 µmol/L A947 for 4 hours ( n = 2 biological replicates). B, Venn diagram indicating A947 treatment–lost regions from ChIP-seq, ATAC-seq, and RNA-seq data in WCM1078. GSEA was performed from 350 overlapping genes. C, Immunoblot of indicated proteins at indicated time upon treatment with 1 µmol/L A947. GAPDH served as loading control. Data are representative of n = 2 independent experiments. D, Dose–response curves with indicated drugs after measurement of proliferation with CellTiter-Glo 2.0 after 7-day treatment ( n = 2 independent experiments).

Article Snippet: Chromatin was prepared from two biological replicates of WCM1078 treated with A858 or A947 (1 μmol/L) for 4 hours, and chromatin immunoprecipitation sequencing (ChIP-seq) assays were then performed using an antibody against TCF7L2 (Cell Signaling Technology, cat #2569, RRID: AB_2199816).

Techniques: ChIP-sequencing, RNA Sequencing, Western Blot, Control

Journal: bioRxiv

Article Title: Single-cell lung eQTL dataset of Asian never-smokers highlights the roles of alveolar cells in lung cancer etiology

doi: 10.64898/2026.03.26.714500

Figure Lengend Snippet: TCF7L2 is a LUAD susceptibility gene involved in Wnt signaling and promoting cellular growth in the AT2 lineage a, Locus zoom plots of eQTL in AT2 cells for TCF7L2 and GWAS (Shi et al EAS, LUAD) p-values of the region (+/-100 kb) around the GWAS top SNP, rs11169089 in the 10q25.2 locus. LD (r 2 ) relationships between the variants are extracted from 1000G EAS Phase 3 v5. b, Allele frequencies difference between East Asian and European from 1000G c, Association between normalized expression of TCF7L2 and rs1196089 is shown as violin plots. 0 = reference allele, while 1 = alternative; red indicates risk allele, while blue indicates protective. Center line denotes the median, while the 25 th and 75 th percentile is marked as the lower and upper line of the box, respectively. Whiskers extend 1.5 times from the 25 th and 75 th percentiles; outliers are represented as dots. The violin width reflects the density. Note the nominal p-value threshold is 1.31e-05. d, Location of guide RNAs targeting the regions around our SNP(s) of interest. Tukey plot shows GAPDH -normalized mRNA levels of TCF7L2 in A549 cell line from 6 replicates from three independent experiments (n =18). Fold change of target gene expression over non-targeting control is shown as median with interquartile range (IQR) in a box. Whiskers extend 1.5 times IQR, with outliers shown as dots. AAVS1 represents a safe harbor site-targeting gRNA. P-values were calculated using a two-tailed Mann Whitney U test. e and f, GSEA analysis in AT2 cells comparing individuals that highly (75 th percentile) vs lowly (25 th percentile) expressed TCF7L2 . The top 5 most enriched gene sets by normalized enrichment score (NES) are shown. A positive NES indicates enrichment of the gene set compared to the reference. Cumulative enrichment score (e) and NES (f) for the top 5 gene sets are shown with a matching color for each gene set in GSEA (e) and NES (f) plots, where circle sizes are scaled to the-log 10 FDR values. g, A representative xCELLigence growth assay result among 5 independent experiments, where mean cell index with SEM of up to 4 replicates over time is shown. Beta estimates (during growth phase) from linear-mixed-effect models comparing shRNA(s) to control are shown; negative sign is indicative of decreased growth rate compared to control. *** denotes p-value < 1e-04 while ** p-value < 1e-03.

Article Snippet: TCF7L2 knockdown was confirmed by western blotting for TCF7L2 (rabbit monoclonal, Cell Signaling #2569S, 1:1000) and GAPDH (mouse monoclonal, Santa Cruz Biotechnology #47724, 1:500).

Techniques: Expressing, Targeted Gene Expression, Control, Two Tailed Test, MANN-WHITNEY, Growth Assay, shRNA