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tbb  (MedChemExpress)


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    MedChemExpress tbb
    A. RCC4 cells were treated with the combination <t>of</t> <t>erastin</t> and <t>TBB</t> for 24hours. Cell viability was measured using CellTiter Glo assay. n=6, error bars = SD. Statistical significance shown at selected erastin doses was determined using Two-Way ANOVA with Dunnett’s multiple comparison of DMSO control vs. TBB concentration . B. Fluorescent microscopy of RCC4 cells incubated with erastin (0.625 µM) and TBB (20 µM) after 24 hours with inclusion of CellTox Green reagent. C . Quantification of CellTox Green fluorescent signal with plate reader of RCC4 cells treated with TBB and erastin (0.625 µM) for 24hours. n=5, error bars = SD. Statistical significance was determined with One-Way ANOVA with Dunnett’s multiple comparison of erastin vs. erastin + TBB samples. D-E. Measuring lipid peroxidation via C11-BODIPY TM staining of RCC4 cells treated with or without TBB (20 µM) and erastin (5 µM) for 16 hours. Quantification of lipid peroxidation shown in E . n=3 error bars = SD. Statistical significance was determined with One-Way ANOVA with Dunnett’s multiple comparison of erastin vs. TBB and erastin vs erastin + TBB samples F. Measurement of GSH/GSSG ratio of RCC4 cells treated with or without erastin (1.25 µM) and with or without TBB (20 µM) for 6 hours. n=4 error bars = SEM. Statistical significance was determined with Two-way ANOVA with Sidák multiple comparisons (TBB vs. DMSO) G. Representative immunoblot showing soluble MESH1 and β-tubulin after treating RCC4 cells with a serial dilution of TBB concentrations (20 µM-1.25 µM) or DMSO control and heating the cells to 50 ℃ for 3 min. H. Quantification of relative fold increase of soluble MESH1. The MESH1 band intensity was normalized to β-tubulin loading control and then normalized to DMSO. n=3 independent experiments. Statistical Significance was assessed with One-Way Anova with Dunnett’s multiple comparisons test for comparing each TBB concentration to DMSO. I. Viability determined with CellTiter Glo of RCC4 cells transfected with siNT, siCK2⍺, or siMESH1 and treated with 10 µM erastin and DMSO or 10 µM TBB for 24 hours. n=6, error bars = SD. Statistical significance was determined using Two-Way ANOVA with Dunnett’s multiple comparison of DMSO vs. TBB. J. Representative images of neurons transfected with YFP+ or Tau and treated with TBB. K . Quantification of neuron survival. One-way ANOVA with Dunnett’s post-hoc test vs. Tau 4R. * = p-value <0.05, ** = p-value < 0.01, *** = p value <0.001, **** p-value < 0.0001
    Tbb, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 94 stars, based on 18 article reviews
    tbb - by Bioz Stars, 2026-04
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    Images

    1) Product Images from "Identification of 4,5,6,7-Tetrabromo-1 H -benzotriazole (TBB) as a Small Molecule MESH1 Inhibitor that Suppresses Ferroptosis"

    Article Title: Identification of 4,5,6,7-Tetrabromo-1 H -benzotriazole (TBB) as a Small Molecule MESH1 Inhibitor that Suppresses Ferroptosis

    Journal: bioRxiv

    doi: 10.64898/2026.02.19.706832

    A. RCC4 cells were treated with the combination of erastin and TBB for 24hours. Cell viability was measured using CellTiter Glo assay. n=6, error bars = SD. Statistical significance shown at selected erastin doses was determined using Two-Way ANOVA with Dunnett’s multiple comparison of DMSO control vs. TBB concentration . B. Fluorescent microscopy of RCC4 cells incubated with erastin (0.625 µM) and TBB (20 µM) after 24 hours with inclusion of CellTox Green reagent. C . Quantification of CellTox Green fluorescent signal with plate reader of RCC4 cells treated with TBB and erastin (0.625 µM) for 24hours. n=5, error bars = SD. Statistical significance was determined with One-Way ANOVA with Dunnett’s multiple comparison of erastin vs. erastin + TBB samples. D-E. Measuring lipid peroxidation via C11-BODIPY TM staining of RCC4 cells treated with or without TBB (20 µM) and erastin (5 µM) for 16 hours. Quantification of lipid peroxidation shown in E . n=3 error bars = SD. Statistical significance was determined with One-Way ANOVA with Dunnett’s multiple comparison of erastin vs. TBB and erastin vs erastin + TBB samples F. Measurement of GSH/GSSG ratio of RCC4 cells treated with or without erastin (1.25 µM) and with or without TBB (20 µM) for 6 hours. n=4 error bars = SEM. Statistical significance was determined with Two-way ANOVA with Sidák multiple comparisons (TBB vs. DMSO) G. Representative immunoblot showing soluble MESH1 and β-tubulin after treating RCC4 cells with a serial dilution of TBB concentrations (20 µM-1.25 µM) or DMSO control and heating the cells to 50 ℃ for 3 min. H. Quantification of relative fold increase of soluble MESH1. The MESH1 band intensity was normalized to β-tubulin loading control and then normalized to DMSO. n=3 independent experiments. Statistical Significance was assessed with One-Way Anova with Dunnett’s multiple comparisons test for comparing each TBB concentration to DMSO. I. Viability determined with CellTiter Glo of RCC4 cells transfected with siNT, siCK2⍺, or siMESH1 and treated with 10 µM erastin and DMSO or 10 µM TBB for 24 hours. n=6, error bars = SD. Statistical significance was determined using Two-Way ANOVA with Dunnett’s multiple comparison of DMSO vs. TBB. J. Representative images of neurons transfected with YFP+ or Tau and treated with TBB. K . Quantification of neuron survival. One-way ANOVA with Dunnett’s post-hoc test vs. Tau 4R. * = p-value <0.05, ** = p-value < 0.01, *** = p value <0.001, **** p-value < 0.0001
    Figure Legend Snippet: A. RCC4 cells were treated with the combination of erastin and TBB for 24hours. Cell viability was measured using CellTiter Glo assay. n=6, error bars = SD. Statistical significance shown at selected erastin doses was determined using Two-Way ANOVA with Dunnett’s multiple comparison of DMSO control vs. TBB concentration . B. Fluorescent microscopy of RCC4 cells incubated with erastin (0.625 µM) and TBB (20 µM) after 24 hours with inclusion of CellTox Green reagent. C . Quantification of CellTox Green fluorescent signal with plate reader of RCC4 cells treated with TBB and erastin (0.625 µM) for 24hours. n=5, error bars = SD. Statistical significance was determined with One-Way ANOVA with Dunnett’s multiple comparison of erastin vs. erastin + TBB samples. D-E. Measuring lipid peroxidation via C11-BODIPY TM staining of RCC4 cells treated with or without TBB (20 µM) and erastin (5 µM) for 16 hours. Quantification of lipid peroxidation shown in E . n=3 error bars = SD. Statistical significance was determined with One-Way ANOVA with Dunnett’s multiple comparison of erastin vs. TBB and erastin vs erastin + TBB samples F. Measurement of GSH/GSSG ratio of RCC4 cells treated with or without erastin (1.25 µM) and with or without TBB (20 µM) for 6 hours. n=4 error bars = SEM. Statistical significance was determined with Two-way ANOVA with Sidák multiple comparisons (TBB vs. DMSO) G. Representative immunoblot showing soluble MESH1 and β-tubulin after treating RCC4 cells with a serial dilution of TBB concentrations (20 µM-1.25 µM) or DMSO control and heating the cells to 50 ℃ for 3 min. H. Quantification of relative fold increase of soluble MESH1. The MESH1 band intensity was normalized to β-tubulin loading control and then normalized to DMSO. n=3 independent experiments. Statistical Significance was assessed with One-Way Anova with Dunnett’s multiple comparisons test for comparing each TBB concentration to DMSO. I. Viability determined with CellTiter Glo of RCC4 cells transfected with siNT, siCK2⍺, or siMESH1 and treated with 10 µM erastin and DMSO or 10 µM TBB for 24 hours. n=6, error bars = SD. Statistical significance was determined using Two-Way ANOVA with Dunnett’s multiple comparison of DMSO vs. TBB. J. Representative images of neurons transfected with YFP+ or Tau and treated with TBB. K . Quantification of neuron survival. One-way ANOVA with Dunnett’s post-hoc test vs. Tau 4R. * = p-value <0.05, ** = p-value < 0.01, *** = p value <0.001, **** p-value < 0.0001

    Techniques Used: Glo Assay, Comparison, Control, Concentration Assay, Microscopy, Incubation, CellTox Assay, Staining, Western Blot, Serial Dilution, Transfection



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    A. RCC4 cells were treated with the combination <t>of</t> <t>erastin</t> and <t>TBB</t> for 24hours. Cell viability was measured using CellTiter Glo assay. n=6, error bars = SD. Statistical significance shown at selected erastin doses was determined using Two-Way ANOVA with Dunnett’s multiple comparison of DMSO control vs. TBB concentration . B. Fluorescent microscopy of RCC4 cells incubated with erastin (0.625 µM) and TBB (20 µM) after 24 hours with inclusion of CellTox Green reagent. C . Quantification of CellTox Green fluorescent signal with plate reader of RCC4 cells treated with TBB and erastin (0.625 µM) for 24hours. n=5, error bars = SD. Statistical significance was determined with One-Way ANOVA with Dunnett’s multiple comparison of erastin vs. erastin + TBB samples. D-E. Measuring lipid peroxidation via C11-BODIPY TM staining of RCC4 cells treated with or without TBB (20 µM) and erastin (5 µM) for 16 hours. Quantification of lipid peroxidation shown in E . n=3 error bars = SD. Statistical significance was determined with One-Way ANOVA with Dunnett’s multiple comparison of erastin vs. TBB and erastin vs erastin + TBB samples F. Measurement of GSH/GSSG ratio of RCC4 cells treated with or without erastin (1.25 µM) and with or without TBB (20 µM) for 6 hours. n=4 error bars = SEM. Statistical significance was determined with Two-way ANOVA with Sidák multiple comparisons (TBB vs. DMSO) G. Representative immunoblot showing soluble MESH1 and β-tubulin after treating RCC4 cells with a serial dilution of TBB concentrations (20 µM-1.25 µM) or DMSO control and heating the cells to 50 ℃ for 3 min. H. Quantification of relative fold increase of soluble MESH1. The MESH1 band intensity was normalized to β-tubulin loading control and then normalized to DMSO. n=3 independent experiments. Statistical Significance was assessed with One-Way Anova with Dunnett’s multiple comparisons test for comparing each TBB concentration to DMSO. I. Viability determined with CellTiter Glo of RCC4 cells transfected with siNT, siCK2⍺, or siMESH1 and treated with 10 µM erastin and DMSO or 10 µM TBB for 24 hours. n=6, error bars = SD. Statistical significance was determined using Two-Way ANOVA with Dunnett’s multiple comparison of DMSO vs. TBB. J. Representative images of neurons transfected with YFP+ or Tau and treated with TBB. K . Quantification of neuron survival. One-way ANOVA with Dunnett’s post-hoc test vs. Tau 4R. * = p-value <0.05, ** = p-value < 0.01, *** = p value <0.001, **** p-value < 0.0001
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    Image Search Results


    A. RCC4 cells were treated with the combination of erastin and TBB for 24hours. Cell viability was measured using CellTiter Glo assay. n=6, error bars = SD. Statistical significance shown at selected erastin doses was determined using Two-Way ANOVA with Dunnett’s multiple comparison of DMSO control vs. TBB concentration . B. Fluorescent microscopy of RCC4 cells incubated with erastin (0.625 µM) and TBB (20 µM) after 24 hours with inclusion of CellTox Green reagent. C . Quantification of CellTox Green fluorescent signal with plate reader of RCC4 cells treated with TBB and erastin (0.625 µM) for 24hours. n=5, error bars = SD. Statistical significance was determined with One-Way ANOVA with Dunnett’s multiple comparison of erastin vs. erastin + TBB samples. D-E. Measuring lipid peroxidation via C11-BODIPY TM staining of RCC4 cells treated with or without TBB (20 µM) and erastin (5 µM) for 16 hours. Quantification of lipid peroxidation shown in E . n=3 error bars = SD. Statistical significance was determined with One-Way ANOVA with Dunnett’s multiple comparison of erastin vs. TBB and erastin vs erastin + TBB samples F. Measurement of GSH/GSSG ratio of RCC4 cells treated with or without erastin (1.25 µM) and with or without TBB (20 µM) for 6 hours. n=4 error bars = SEM. Statistical significance was determined with Two-way ANOVA with Sidák multiple comparisons (TBB vs. DMSO) G. Representative immunoblot showing soluble MESH1 and β-tubulin after treating RCC4 cells with a serial dilution of TBB concentrations (20 µM-1.25 µM) or DMSO control and heating the cells to 50 ℃ for 3 min. H. Quantification of relative fold increase of soluble MESH1. The MESH1 band intensity was normalized to β-tubulin loading control and then normalized to DMSO. n=3 independent experiments. Statistical Significance was assessed with One-Way Anova with Dunnett’s multiple comparisons test for comparing each TBB concentration to DMSO. I. Viability determined with CellTiter Glo of RCC4 cells transfected with siNT, siCK2⍺, or siMESH1 and treated with 10 µM erastin and DMSO or 10 µM TBB for 24 hours. n=6, error bars = SD. Statistical significance was determined using Two-Way ANOVA with Dunnett’s multiple comparison of DMSO vs. TBB. J. Representative images of neurons transfected with YFP+ or Tau and treated with TBB. K . Quantification of neuron survival. One-way ANOVA with Dunnett’s post-hoc test vs. Tau 4R. * = p-value <0.05, ** = p-value < 0.01, *** = p value <0.001, **** p-value < 0.0001

    Journal: bioRxiv

    Article Title: Identification of 4,5,6,7-Tetrabromo-1 H -benzotriazole (TBB) as a Small Molecule MESH1 Inhibitor that Suppresses Ferroptosis

    doi: 10.64898/2026.02.19.706832

    Figure Lengend Snippet: A. RCC4 cells were treated with the combination of erastin and TBB for 24hours. Cell viability was measured using CellTiter Glo assay. n=6, error bars = SD. Statistical significance shown at selected erastin doses was determined using Two-Way ANOVA with Dunnett’s multiple comparison of DMSO control vs. TBB concentration . B. Fluorescent microscopy of RCC4 cells incubated with erastin (0.625 µM) and TBB (20 µM) after 24 hours with inclusion of CellTox Green reagent. C . Quantification of CellTox Green fluorescent signal with plate reader of RCC4 cells treated with TBB and erastin (0.625 µM) for 24hours. n=5, error bars = SD. Statistical significance was determined with One-Way ANOVA with Dunnett’s multiple comparison of erastin vs. erastin + TBB samples. D-E. Measuring lipid peroxidation via C11-BODIPY TM staining of RCC4 cells treated with or without TBB (20 µM) and erastin (5 µM) for 16 hours. Quantification of lipid peroxidation shown in E . n=3 error bars = SD. Statistical significance was determined with One-Way ANOVA with Dunnett’s multiple comparison of erastin vs. TBB and erastin vs erastin + TBB samples F. Measurement of GSH/GSSG ratio of RCC4 cells treated with or without erastin (1.25 µM) and with or without TBB (20 µM) for 6 hours. n=4 error bars = SEM. Statistical significance was determined with Two-way ANOVA with Sidák multiple comparisons (TBB vs. DMSO) G. Representative immunoblot showing soluble MESH1 and β-tubulin after treating RCC4 cells with a serial dilution of TBB concentrations (20 µM-1.25 µM) or DMSO control and heating the cells to 50 ℃ for 3 min. H. Quantification of relative fold increase of soluble MESH1. The MESH1 band intensity was normalized to β-tubulin loading control and then normalized to DMSO. n=3 independent experiments. Statistical Significance was assessed with One-Way Anova with Dunnett’s multiple comparisons test for comparing each TBB concentration to DMSO. I. Viability determined with CellTiter Glo of RCC4 cells transfected with siNT, siCK2⍺, or siMESH1 and treated with 10 µM erastin and DMSO or 10 µM TBB for 24 hours. n=6, error bars = SD. Statistical significance was determined using Two-Way ANOVA with Dunnett’s multiple comparison of DMSO vs. TBB. J. Representative images of neurons transfected with YFP+ or Tau and treated with TBB. K . Quantification of neuron survival. One-way ANOVA with Dunnett’s post-hoc test vs. Tau 4R. * = p-value <0.05, ** = p-value < 0.01, *** = p value <0.001, **** p-value < 0.0001

    Article Snippet: Erastin (MedChem Express:HY-15763), IKE (MedChem Express: HY-114481), TBB (MedChem Express: HY-14394), TFBt (Enamine; EN300-6487228), TCBt (Sigma; COMH04236427), TIBi (AABlocks; AA01X6YQ), 5-monoBBt (Enamine; EN300-137666), 4-monoBBt (Enamine; EN300-137659), 5,6-diBBt (Enamine; EN300-6775516), 5-monoIBt (Enamine; EN300-2951206), 4-F,5-monoBBt (Enamine; EN300-5495791), 6,7diBQX (Enamine; EN300-19483685), DMAT (MedChem Express: HY-15535), TBCA (MedChem Express: HY-110052), DRB (MedChem Express: HY-14392), TBBi (Cayman Chemical, 18886), DPPH (Thermo Scientific, 044150.MD), Trolox (Thermo Scientific, 218940010), Ferrozine (Sigma, 160601), DFO (Sigma, D9533)

    Techniques: Glo Assay, Comparison, Control, Concentration Assay, Microscopy, Incubation, CellTox Assay, Staining, Western Blot, Serial Dilution, Transfection