Virus:
Recombinant:
Article Title: The xCT/CD98 complex suppresses ferroptosis in pan-cancer via a non-canonical RACK1-mediated iron homeostasis pathway.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-RACK1 Santa Cruz Biotechnology Cat# sc-17754; RRID: AB_2247471 Mouse monoclonal anti-CD98 Santa Cruz Biotechnology Cat# sc-376815; RRID: AB_2938854 Rabbit polyclonal anti-CD98 Proteintech Cat# 15193-1-AP; RRID: AB_2254909 Rabbit polyclonal anti-SLC40A1 Proteintech Cat# 26601-1-AP; RRID: AB_2880571 Mouse monoclonal anti- Ubiquitin Cell Signaling Technology Cat# 3936S; RRID: AB_3717987 Mouse monoclonal anti-ferritin heavy chain Santa Cruz Biotechnology Cat# sc-376594; RRID: AB_11150859 Rabbit polyclonal anti-NCOA4 Abcam Cat# ab86707; RRID: AB_1925236 Rabbit polyclonal anti-CD71 proteintech Cat# 10084-2-AP; RRID: AB_2240403 Mouse anti-GAPDH Fdbio FD0063; RRID: AB_2934268 Rabbit polyclonal anti-DMT1 proteintech Cat# 20507-1-AP; RRID: AB_10694284 Rabbit monoclonal anti-xCT/SLC7A11 Cell Signaling Technology Cat# 12691S; RRID: AB_3717988 Rabbit monoclonal anti- Glutathione Peroxidase 4 Abcam Cat# ab125066; RRID: AB_10973901 Mouse monoclonal anti-HA-Tag Santa Cruz Biotechnology Cat# sc-7392; RRID: AB_627809 Mouse monoclonal anti-Flag Tag Bioss Cat# bsm-33346M; RRID: AB_3083063 Rabbit polyclonal anti-MYC-tag proteintech Cat# 16286-1-AP; RRID: AB_11182162 Mouse monoclonal anti-Myc-tag OriGene Cat# TA150121; RRID: AB_2622266 Biological samples Breast tumor and paracancerous tissue chip Sir Run Run Shaw Hospital N/A Chemicals, peptides, and recombinant proteins Sorafenib CSNpharm CSN10381 Erastin MCE HY-15763 Tamoxifen MCE HY-13757A trifluoroacetic acid Sigma 76-05-1 Ferrostatin-1 Selleck S7243 Deferoxamine MCE HY-B1625 MG132 Selleck S2619 cycloheximide Selleck S7418 chloroquine Selleck S6999 Doxorubicin Sigma D1515, Harringtonolide MACKIN H962769 Critical commercial assays PTMScan phosphotyrosine Rabbit mAb (P-Tyr-1000) Kit Cell Signaling Technology 8803 PTMScan Ubiquitin Remnant Motif (K-ε-GG) Kit Cell Signaling Technology 5562 PTMScan Acetyl-Lysine Motif [Ac-K] Kit Cell Signaling Technology 13416 protein A/G Santa Cruz Biotechnology sc-2003 RNAfast1000 fastagen 220011 RT Master Mix Takara RR036A TB Green® Premix Ex TaqTM Takara RR420 Cell Counting Kit-8 APEXBT K1018 FITC-transferrin Rockland Immunochemicals 009–0234 BODIPY 581/591 C11 ThermoFisher D3861 FerroOrange Dojindo F374 Glutathione Assay Kit Beyotime S0052 Lipofectamine ThermoFisher 56532 (Continued on next page) Cell Reports 44, 116563, November 25, 2025 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Neofect NEOFECT TF20121201 Iron Assay Kit DOJINDO I291 In Situ Red Starter Kit Mouse/Rabbit Duolink® DUO92101-1KT Deposited data The mass spectrometry proteomics data of Sorafinib Zhu et al.15 PXD045576 Experimental models: Cell lines Human: MDA-MB-231 cell line Chinese Academy of Sciences N/A Human: BT549 cell line Chinese Academy of Sciences N/A Human: HS578T cell line Chinese Academy of Sciences N/A Human: MCF7 cell line Chinese Academy of Sciences N/A Human: MHCC97-L MeisenCTCC CTCC-400-0194 Human: Panc01 cell line Chinese Academy of Sciences N/A Human: PC-3 MeisenCTCC CTCC-0337 Human: A2780 MeisenCTCC CTCC-0402 Human: A549 MeisenCTCC CTCC-0336 Human: MG803 cell line Chinese Academy of Sciences N/A Experimental models: Organisms/strains Mouse: 4-week-old female nude mice Hangzhou Medical College N/A Oligonucleotides siRNA targeting sequence: RACK1 #1: GUCUCUGGA UCUCGAGAUA; #2: GGAUGAGACCAACUAUGGA This paper N/A siRNA targeting sequence: TRIM21 #1: GCAUGGUC UCCUUCUACAA; #2: CGCAGAGUUUG UGCAGCAA This paper N/A siRNA targeting sequence: FPN1 #1: GGUGUGAAAG ACUCUAACA This paper N/A Primer: GAPDH Forward: TGACTTCAACAGCGACA CCCA This paper N/A Primer: RACK1 Forward: TGAGCAGATGACCCTT CGTG This paper N/A Primer: TRIM21 Forward: GTCCTGGAAAGGAGTGA GTCC This paper N/A Primer: FPN1 Forward: CTACTTGGGGAGATCGGA TGT This paper N/A Primer: FTH1 Forward: TGAAGCTGCAGAACCA ACGAGG This paper N/A Primer: DMT1 Forward: AGCTCCACCATGACAG GAACCT This paper N/A Primer: TFRC Forward: ATCGGTTGGTGCCACT GAATGG This paper N/A Recombinant DNA pCDNA3.1-RACK1-Myc-HisA This paper N/A pCDNA3.1-RACK1 K280R-Myc-HisA This paper N/A PCDNA3.1- Myc-HisA TSINGKE N/A pCMV-TRIM21-FLAG-C This paper N/A pCMV- FLAG-C TSINGKE N/A Software and algorithms R Yu et al.44 https://www.r-project.org/ GSEA analysis Zhou et al.45 https://www.gsea-msigdb.org/ gsea/index.jsp (Continued on next page) 18 Cell Reports 44, 116563, November 25, 2025
Western Blot:Article Title: β-arrestin1/YAP/mutant p53 complexes orchestrate the endothelin A receptor signaling in high-grade serous ovarian cancer
Article Snippet: .. Antibodies used for Immunoblotting Antigen Diluition Manufacturer PCNA (F-2) sc-25280 1:200 Santa Cruz Biotechology Tubulin (DM1A) sc-32293 1:201 Santa Cruz Biotechology ETAR ab84673 1:1000 Abcam ETBR ab39960 1:1000 Abcam pYAP (S127) D9W2I cat no. 13008 1:1000 Cell Signaling YAP 1A12 cat. no. 12395 1:1000 Cell Signaling pTAZ (S89) E1X9C cat. no. 59971 1:1000 Cell Signaling TAZ cat. no. HPA007415 1:250 Sigma Aldrich β-arrestin 1 E274 ab32099 1:500 Abcam FLAG cat. no. 2368 1:1000 Cell Signaling AU5 ab24576 1:500 Abcam Trio (H-120) sc-28564 1:200 Santa Cruz Biotechology pTyrosincat. no. 9411 1:2000 Cell Signaling RhoA-GTP 1:1000 Cytoskeleton, Inc. RhoA, B, C clone 55 cat. no. 05-778 1:500 Millipore pLATS1 (T1079) D57D3 cat. no. 8654 1:1000 Cell Signaling LATS1 cat. no. A300-477A 1:5000 Bethyl Myc 9E10 cat. no. TA150121 1:500 OriGene p53 (DO1) sc-126 1:200 Santa Cruz Biotechology Cleaved-PARP cat. no. 9541 1:1000 Cell Signaling GNAQ ab75825 1:1000 Abcam TEAD (TEF-3, N-G2) sc-101184 1:200 Santa Cruz Biotechology α-actin (1A4) sc-32251 1:200 Santa Cruz Biotechology HSP70 (3A3) sc-32239 1:200 Santa Cruz Biotechology Supplementary Table 2: Primer List Primers used for RT-qPCR experiments Target gene Primer sequences CYR61 F 5’-CAGGACTGTGAAGATGCGGT-3’ CYR61 R 5’-GCCTGTAGAAGGGAAACGCT-3’ CTGF F 5’-AGGAGTGGGTGTGTGACGA-3’ CTGF R 5’-CCAGGCAGTTGGCTCTAATC-3’ ANKRD1 F 5’-AGTAGAGGAACTGGTCACTGG-3’ ANKRD1 R 5’-TGGGCTAGAAGTGTCTTCAGA-3’ EDN1 F 5’-CCAAGAGAGCCTTGGAGAAT-3’ EDN1 R 5’-TGTCTTCAGCCCTGAGTTCTT-3’ EDNRA F 5’-GGGATCACCGTCCTCAACCT-3’ EDNRA R 5’-CAGGAATGGCCAGGATAAAGG-3’ ARRB1 F 5’-GTGAAAGTGAAGCTGGTGGTGTC-3’ ARRB1 R 5’-TTCATGCCTTTCAGCCGCTG-3’ YAP F 5’-GCAAATTCTCCAAAATGTCAGG-3’ YAP R 5’-CGGGAGAAGACACTGGATTT-3’ CYCLOPHILIN-A F 5’-TTCATCTGCACTGCCAAGAC-3’ CYCLOPHILIN-A R 5’-TCGAGTTGTCCACAGTCAGC-3’ Cdk1 F 5'-GGAAGGGGTTCCTAGTACTGC-3' Cdk1 R 5'-TGGAATCCTGCATAAGCACA-3' CCNA2 F 5'-CCATACCTCAAGTATTTGCCATCA-3' CCNA2 R 5'-AGCTTTGTCCCGTGACTGTGT-3' Primers used in ChIP experiments Promoter Primer sequences ET-1 F 5′-CAGCTTGCAAAGGGGAAGCG-3′ ET-1 R 5′- TCCGACTTTATTCCAGCCCC -3’ CTGF F 5’-CAATCCGGTGTGAGTTGATG-3’ CTGF R 5’-GCCAATGAGCTGAATGGAGT-3’ ANKRD1 F 5'-GAGGGGAGGACAAGCTAACC-3’ ANKRD1 R 5’-CGATGTGATCACCACCAAAG-3’ GAPDH F 5’-CGGGATTGTCTGCCCTAATTAT-3’ GAPDH R 5’-GCACGGAAGGTCACGATGT-3’. ..
Article Title: Hypoxia induces arginase II expression and increases viable human pulmonary artery smooth muscle cell numbers via AMPKα 1 signaling
Article Snippet: .. Protein was assayed with evidence of specificity for arginase II ( 4 ) (1:500, cat. no. sc-20151, lot no. A3013; Santa Cruz), cleaved caspase 3 ( 28 ) (1:1,000, cat. no. 9664, lot no. 19, Cell Signaling, Danvers, MA), total caspase 3 ( 28 ) (1:1,000, cat. no. 9622, lot no. 18; Cell Signaling), AMPKα 1 ( 40 ) (1:1,000, cat. no. 2795, lot no. 3; Cell Signaling), AMPKα 2 ( 40 ) (1:1,000, cat. no. 2757, lot no. 2; Cell Signaling), p-AMPKα 1/2 (Thr-172) ( 23 ) (1:500, cat. no. sc-33524, lot no. C0813; Santa Cruz), total AMPK ( 22 ) (1:500, cat. no. sc-25792; lot no. A2013, Santa Cruz), myc ( 27 ) (1:1,000, cat. no. TA150121, lot no. A002; OriGene, Rockville, MD), p-ACC (Ser-79) ( 29 ) (1:1,000, cat. no. 11818, lot no. 1; Cell Signaling), total ACC ( 1 ) (1:1,000, cat. no. 3676, lot no. 8; Cell Signaling), and β-actin (1:10,000, cat. no. 3700, lot no. 8, Cell Signaling) by Western blot analysis, as previously described ( 32 , 33 ). .. Protein bands were visualized using enhanced chemiluminescence (Luminata Classico/Forte Western HRP Substrate; EMD Millipore Corporation, Billerica, MA) and were quantified using densitometry (UVP BioImaging Systems, Upland, CA).
Quantitative RT-PCR:Article Title: β-arrestin1/YAP/mutant p53 complexes orchestrate the endothelin A receptor signaling in high-grade serous ovarian cancer
Article Snippet: .. Antibodies used for Immunoblotting Antigen Diluition Manufacturer PCNA (F-2) sc-25280 1:200 Santa Cruz Biotechology Tubulin (DM1A) sc-32293 1:201 Santa Cruz Biotechology ETAR ab84673 1:1000 Abcam ETBR ab39960 1:1000 Abcam pYAP (S127) D9W2I cat no. 13008 1:1000 Cell Signaling YAP 1A12 cat. no. 12395 1:1000 Cell Signaling pTAZ (S89) E1X9C cat. no. 59971 1:1000 Cell Signaling TAZ cat. no. HPA007415 1:250 Sigma Aldrich β-arrestin 1 E274 ab32099 1:500 Abcam FLAG cat. no. 2368 1:1000 Cell Signaling AU5 ab24576 1:500 Abcam Trio (H-120) sc-28564 1:200 Santa Cruz Biotechology pTyrosincat. no. 9411 1:2000 Cell Signaling RhoA-GTP 1:1000 Cytoskeleton, Inc. RhoA, B, C clone 55 cat. no. 05-778 1:500 Millipore pLATS1 (T1079) D57D3 cat. no. 8654 1:1000 Cell Signaling LATS1 cat. no. A300-477A 1:5000 Bethyl Myc 9E10 cat. no. TA150121 1:500 OriGene p53 (DO1) sc-126 1:200 Santa Cruz Biotechology Cleaved-PARP cat. no. 9541 1:1000 Cell Signaling GNAQ ab75825 1:1000 Abcam TEAD (TEF-3, N-G2) sc-101184 1:200 Santa Cruz Biotechology α-actin (1A4) sc-32251 1:200 Santa Cruz Biotechology HSP70 (3A3) sc-32239 1:200 Santa Cruz Biotechology Supplementary Table 2: Primer List Primers used for RT-qPCR experiments Target gene Primer sequences CYR61 F 5’-CAGGACTGTGAAGATGCGGT-3’ CYR61 R 5’-GCCTGTAGAAGGGAAACGCT-3’ CTGF F 5’-AGGAGTGGGTGTGTGACGA-3’ CTGF R 5’-CCAGGCAGTTGGCTCTAATC-3’ ANKRD1 F 5’-AGTAGAGGAACTGGTCACTGG-3’ ANKRD1 R 5’-TGGGCTAGAAGTGTCTTCAGA-3’ EDN1 F 5’-CCAAGAGAGCCTTGGAGAAT-3’ EDN1 R 5’-TGTCTTCAGCCCTGAGTTCTT-3’ EDNRA F 5’-GGGATCACCGTCCTCAACCT-3’ EDNRA R 5’-CAGGAATGGCCAGGATAAAGG-3’ ARRB1 F 5’-GTGAAAGTGAAGCTGGTGGTGTC-3’ ARRB1 R 5’-TTCATGCCTTTCAGCCGCTG-3’ YAP F 5’-GCAAATTCTCCAAAATGTCAGG-3’ YAP R 5’-CGGGAGAAGACACTGGATTT-3’ CYCLOPHILIN-A F 5’-TTCATCTGCACTGCCAAGAC-3’ CYCLOPHILIN-A R 5’-TCGAGTTGTCCACAGTCAGC-3’ Cdk1 F 5'-GGAAGGGGTTCCTAGTACTGC-3' Cdk1 R 5'-TGGAATCCTGCATAAGCACA-3' CCNA2 F 5'-CCATACCTCAAGTATTTGCCATCA-3' CCNA2 R 5'-AGCTTTGTCCCGTGACTGTGT-3' Primers used in ChIP experiments Promoter Primer sequences ET-1 F 5′-CAGCTTGCAAAGGGGAAGCG-3′ ET-1 R 5′- TCCGACTTTATTCCAGCCCC -3’ CTGF F 5’-CAATCCGGTGTGAGTTGATG-3’ CTGF R 5’-GCCAATGAGCTGAATGGAGT-3’ ANKRD1 F 5'-GAGGGGAGGACAAGCTAACC-3’ ANKRD1 R 5’-CGATGTGATCACCACCAAAG-3’ GAPDH F 5’-CGGGATTGTCTGCCCTAATTAT-3’ GAPDH R 5’-GCACGGAAGGTCACGATGT-3’. ..
Chromatin Immunoprecipitation:Article Title: β-arrestin1/YAP/mutant p53 complexes orchestrate the endothelin A receptor signaling in high-grade serous ovarian cancer
Article Snippet: .. Antibodies used for Immunoblotting Antigen Diluition Manufacturer PCNA (F-2) sc-25280 1:200 Santa Cruz Biotechology Tubulin (DM1A) sc-32293 1:201 Santa Cruz Biotechology ETAR ab84673 1:1000 Abcam ETBR ab39960 1:1000 Abcam pYAP (S127) D9W2I cat no. 13008 1:1000 Cell Signaling YAP 1A12 cat. no. 12395 1:1000 Cell Signaling pTAZ (S89) E1X9C cat. no. 59971 1:1000 Cell Signaling TAZ cat. no. HPA007415 1:250 Sigma Aldrich β-arrestin 1 E274 ab32099 1:500 Abcam FLAG cat. no. 2368 1:1000 Cell Signaling AU5 ab24576 1:500 Abcam Trio (H-120) sc-28564 1:200 Santa Cruz Biotechology pTyrosincat. no. 9411 1:2000 Cell Signaling RhoA-GTP 1:1000 Cytoskeleton, Inc. RhoA, B, C clone 55 cat. no. 05-778 1:500 Millipore pLATS1 (T1079) D57D3 cat. no. 8654 1:1000 Cell Signaling LATS1 cat. no. A300-477A 1:5000 Bethyl Myc 9E10 cat. no. TA150121 1:500 OriGene p53 (DO1) sc-126 1:200 Santa Cruz Biotechology Cleaved-PARP cat. no. 9541 1:1000 Cell Signaling GNAQ ab75825 1:1000 Abcam TEAD (TEF-3, N-G2) sc-101184 1:200 Santa Cruz Biotechology α-actin (1A4) sc-32251 1:200 Santa Cruz Biotechology HSP70 (3A3) sc-32239 1:200 Santa Cruz Biotechology Supplementary Table 2: Primer List Primers used for RT-qPCR experiments Target gene Primer sequences CYR61 F 5’-CAGGACTGTGAAGATGCGGT-3’ CYR61 R 5’-GCCTGTAGAAGGGAAACGCT-3’ CTGF F 5’-AGGAGTGGGTGTGTGACGA-3’ CTGF R 5’-CCAGGCAGTTGGCTCTAATC-3’ ANKRD1 F 5’-AGTAGAGGAACTGGTCACTGG-3’ ANKRD1 R 5’-TGGGCTAGAAGTGTCTTCAGA-3’ EDN1 F 5’-CCAAGAGAGCCTTGGAGAAT-3’ EDN1 R 5’-TGTCTTCAGCCCTGAGTTCTT-3’ EDNRA F 5’-GGGATCACCGTCCTCAACCT-3’ EDNRA R 5’-CAGGAATGGCCAGGATAAAGG-3’ ARRB1 F 5’-GTGAAAGTGAAGCTGGTGGTGTC-3’ ARRB1 R 5’-TTCATGCCTTTCAGCCGCTG-3’ YAP F 5’-GCAAATTCTCCAAAATGTCAGG-3’ YAP R 5’-CGGGAGAAGACACTGGATTT-3’ CYCLOPHILIN-A F 5’-TTCATCTGCACTGCCAAGAC-3’ CYCLOPHILIN-A R 5’-TCGAGTTGTCCACAGTCAGC-3’ Cdk1 F 5'-GGAAGGGGTTCCTAGTACTGC-3' Cdk1 R 5'-TGGAATCCTGCATAAGCACA-3' CCNA2 F 5'-CCATACCTCAAGTATTTGCCATCA-3' CCNA2 R 5'-AGCTTTGTCCCGTGACTGTGT-3' Primers used in ChIP experiments Promoter Primer sequences ET-1 F 5′-CAGCTTGCAAAGGGGAAGCG-3′ ET-1 R 5′- TCCGACTTTATTCCAGCCCC -3’ CTGF F 5’-CAATCCGGTGTGAGTTGATG-3’ CTGF R 5’-GCCAATGAGCTGAATGGAGT-3’ ANKRD1 F 5'-GAGGGGAGGACAAGCTAACC-3’ ANKRD1 R 5’-CGATGTGATCACCACCAAAG-3’ GAPDH F 5’-CGGGATTGTCTGCCCTAATTAT-3’ GAPDH R 5’-GCACGGAAGGTCACGATGT-3’. ..
Enzyme-linked Immunosorbent Assay:
other:
Ubiquitin Proteomics:Article Title: The xCT/CD98 complex suppresses ferroptosis in pan-cancer via a non-canonical RACK1-mediated iron homeostasis pathway.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-RACK1 Santa Cruz Biotechnology Cat# sc-17754; RRID: AB_2247471 Mouse monoclonal anti-CD98 Santa Cruz Biotechnology Cat# sc-376815; RRID: AB_2938854 Rabbit polyclonal anti-CD98 Proteintech Cat# 15193-1-AP; RRID: AB_2254909 Rabbit polyclonal anti-SLC40A1 Proteintech Cat# 26601-1-AP; RRID: AB_2880571 Mouse monoclonal anti- Ubiquitin Cell Signaling Technology Cat# 3936S; RRID: AB_3717987 Mouse monoclonal anti-ferritin heavy chain Santa Cruz Biotechnology Cat# sc-376594; RRID: AB_11150859 Rabbit polyclonal anti-NCOA4 Abcam Cat# ab86707; RRID: AB_1925236 Rabbit polyclonal anti-CD71 proteintech Cat# 10084-2-AP; RRID: AB_2240403 Mouse anti-GAPDH Fdbio FD0063; RRID: AB_2934268 Rabbit polyclonal anti-DMT1 proteintech Cat# 20507-1-AP; RRID: AB_10694284 Rabbit monoclonal anti-xCT/SLC7A11 Cell Signaling Technology Cat# 12691S; RRID: AB_3717988 Rabbit monoclonal anti- Glutathione Peroxidase 4 Abcam Cat# ab125066; RRID: AB_10973901 Mouse monoclonal anti-HA-Tag Santa Cruz Biotechnology Cat# sc-7392; RRID: AB_627809 Mouse monoclonal anti-Flag Tag Bioss Cat# bsm-33346M; RRID: AB_3083063 Rabbit polyclonal anti-MYC-tag proteintech Cat# 16286-1-AP; RRID: AB_11182162 Mouse monoclonal anti-Myc-tag OriGene Cat# TA150121; RRID: AB_2622266 Biological samples Breast tumor and paracancerous tissue chip Sir Run Run Shaw Hospital N/A Chemicals, peptides, and recombinant proteins Sorafenib CSNpharm CSN10381 Erastin MCE HY-15763 Tamoxifen MCE HY-13757A trifluoroacetic acid Sigma 76-05-1 Ferrostatin-1 Selleck S7243 Deferoxamine MCE HY-B1625 MG132 Selleck S2619 cycloheximide Selleck S7418 chloroquine Selleck S6999 Doxorubicin Sigma D1515, Harringtonolide MACKIN H962769 Critical commercial assays PTMScan phosphotyrosine Rabbit mAb (P-Tyr-1000) Kit Cell Signaling Technology 8803 PTMScan Ubiquitin Remnant Motif (K-ε-GG) Kit Cell Signaling Technology 5562 PTMScan Acetyl-Lysine Motif [Ac-K] Kit Cell Signaling Technology 13416 protein A/G Santa Cruz Biotechnology sc-2003 RNAfast1000 fastagen 220011 RT Master Mix Takara RR036A TB Green® Premix Ex TaqTM Takara RR420 Cell Counting Kit-8 APEXBT K1018 FITC-transferrin Rockland Immunochemicals 009–0234 BODIPY 581/591 C11 ThermoFisher D3861 FerroOrange Dojindo F374 Glutathione Assay Kit Beyotime S0052 Lipofectamine ThermoFisher 56532 (Continued on next page) Cell Reports 44, 116563, November 25, 2025 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Neofect NEOFECT TF20121201 Iron Assay Kit DOJINDO I291 In Situ Red Starter Kit Mouse/Rabbit Duolink® DUO92101-1KT Deposited data The mass spectrometry proteomics data of Sorafinib Zhu et al.15 PXD045576 Experimental models: Cell lines Human: MDA-MB-231 cell line Chinese Academy of Sciences N/A Human: BT549 cell line Chinese Academy of Sciences N/A Human: HS578T cell line Chinese Academy of Sciences N/A Human: MCF7 cell line Chinese Academy of Sciences N/A Human: MHCC97-L MeisenCTCC CTCC-400-0194 Human: Panc01 cell line Chinese Academy of Sciences N/A Human: PC-3 MeisenCTCC CTCC-0337 Human: A2780 MeisenCTCC CTCC-0402 Human: A549 MeisenCTCC CTCC-0336 Human: MG803 cell line Chinese Academy of Sciences N/A Experimental models: Organisms/strains Mouse: 4-week-old female nude mice Hangzhou Medical College N/A Oligonucleotides siRNA targeting sequence: RACK1 #1: GUCUCUGGA UCUCGAGAUA; #2: GGAUGAGACCAACUAUGGA This paper N/A siRNA targeting sequence: TRIM21 #1: GCAUGGUC UCCUUCUACAA; #2: CGCAGAGUUUG UGCAGCAA This paper N/A siRNA targeting sequence: FPN1 #1: GGUGUGAAAG ACUCUAACA This paper N/A Primer: GAPDH Forward: TGACTTCAACAGCGACA CCCA This paper N/A Primer: RACK1 Forward: TGAGCAGATGACCCTT CGTG This paper N/A Primer: TRIM21 Forward: GTCCTGGAAAGGAGTGA GTCC This paper N/A Primer: FPN1 Forward: CTACTTGGGGAGATCGGA TGT This paper N/A Primer: FTH1 Forward: TGAAGCTGCAGAACCA ACGAGG This paper N/A Primer: DMT1 Forward: AGCTCCACCATGACAG GAACCT This paper N/A Primer: TFRC Forward: ATCGGTTGGTGCCACT GAATGG This paper N/A Recombinant DNA pCDNA3.1-RACK1-Myc-HisA This paper N/A pCDNA3.1-RACK1 K280R-Myc-HisA This paper N/A PCDNA3.1- Myc-HisA TSINGKE N/A pCMV-TRIM21-FLAG-C This paper N/A pCMV- FLAG-C TSINGKE N/A Software and algorithms R Yu et al.44 https://www.r-project.org/ GSEA analysis Zhou et al.45 https://www.gsea-msigdb.org/ gsea/index.jsp (Continued on next page) 18 Cell Reports 44, 116563, November 25, 2025
CCK-8 Assay:Article Title: The xCT/CD98 complex suppresses ferroptosis in pan-cancer via a non-canonical RACK1-mediated iron homeostasis pathway.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-RACK1 Santa Cruz Biotechnology Cat# sc-17754; RRID: AB_2247471 Mouse monoclonal anti-CD98 Santa Cruz Biotechnology Cat# sc-376815; RRID: AB_2938854 Rabbit polyclonal anti-CD98 Proteintech Cat# 15193-1-AP; RRID: AB_2254909 Rabbit polyclonal anti-SLC40A1 Proteintech Cat# 26601-1-AP; RRID: AB_2880571 Mouse monoclonal anti- Ubiquitin Cell Signaling Technology Cat# 3936S; RRID: AB_3717987 Mouse monoclonal anti-ferritin heavy chain Santa Cruz Biotechnology Cat# sc-376594; RRID: AB_11150859 Rabbit polyclonal anti-NCOA4 Abcam Cat# ab86707; RRID: AB_1925236 Rabbit polyclonal anti-CD71 proteintech Cat# 10084-2-AP; RRID: AB_2240403 Mouse anti-GAPDH Fdbio FD0063; RRID: AB_2934268 Rabbit polyclonal anti-DMT1 proteintech Cat# 20507-1-AP; RRID: AB_10694284 Rabbit monoclonal anti-xCT/SLC7A11 Cell Signaling Technology Cat# 12691S; RRID: AB_3717988 Rabbit monoclonal anti- Glutathione Peroxidase 4 Abcam Cat# ab125066; RRID: AB_10973901 Mouse monoclonal anti-HA-Tag Santa Cruz Biotechnology Cat# sc-7392; RRID: AB_627809 Mouse monoclonal anti-Flag Tag Bioss Cat# bsm-33346M; RRID: AB_3083063 Rabbit polyclonal anti-MYC-tag proteintech Cat# 16286-1-AP; RRID: AB_11182162 Mouse monoclonal anti-Myc-tag OriGene Cat# TA150121; RRID: AB_2622266 Biological samples Breast tumor and paracancerous tissue chip Sir Run Run Shaw Hospital N/A Chemicals, peptides, and recombinant proteins Sorafenib CSNpharm CSN10381 Erastin MCE HY-15763 Tamoxifen MCE HY-13757A trifluoroacetic acid Sigma 76-05-1 Ferrostatin-1 Selleck S7243 Deferoxamine MCE HY-B1625 MG132 Selleck S2619 cycloheximide Selleck S7418 chloroquine Selleck S6999 Doxorubicin Sigma D1515, Harringtonolide MACKIN H962769 Critical commercial assays PTMScan phosphotyrosine Rabbit mAb (P-Tyr-1000) Kit Cell Signaling Technology 8803 PTMScan Ubiquitin Remnant Motif (K-ε-GG) Kit Cell Signaling Technology 5562 PTMScan Acetyl-Lysine Motif [Ac-K] Kit Cell Signaling Technology 13416 protein A/G Santa Cruz Biotechnology sc-2003 RNAfast1000 fastagen 220011 RT Master Mix Takara RR036A TB Green® Premix Ex TaqTM Takara RR420 Cell Counting Kit-8 APEXBT K1018 FITC-transferrin Rockland Immunochemicals 009–0234 BODIPY 581/591 C11 ThermoFisher D3861 FerroOrange Dojindo F374 Glutathione Assay Kit Beyotime S0052 Lipofectamine ThermoFisher 56532 (Continued on next page) Cell Reports 44, 116563, November 25, 2025 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Neofect NEOFECT TF20121201 Iron Assay Kit DOJINDO I291 In Situ Red Starter Kit Mouse/Rabbit Duolink® DUO92101-1KT Deposited data The mass spectrometry proteomics data of Sorafinib Zhu et al.15 PXD045576 Experimental models: Cell lines Human: MDA-MB-231 cell line Chinese Academy of Sciences N/A Human: BT549 cell line Chinese Academy of Sciences N/A Human: HS578T cell line Chinese Academy of Sciences N/A Human: MCF7 cell line Chinese Academy of Sciences N/A Human: MHCC97-L MeisenCTCC CTCC-400-0194 Human: Panc01 cell line Chinese Academy of Sciences N/A Human: PC-3 MeisenCTCC CTCC-0337 Human: A2780 MeisenCTCC CTCC-0402 Human: A549 MeisenCTCC CTCC-0336 Human: MG803 cell line Chinese Academy of Sciences N/A Experimental models: Organisms/strains Mouse: 4-week-old female nude mice Hangzhou Medical College N/A Oligonucleotides siRNA targeting sequence: RACK1 #1: GUCUCUGGA UCUCGAGAUA; #2: GGAUGAGACCAACUAUGGA This paper N/A siRNA targeting sequence: TRIM21 #1: GCAUGGUC UCCUUCUACAA; #2: CGCAGAGUUUG UGCAGCAA This paper N/A siRNA targeting sequence: FPN1 #1: GGUGUGAAAG ACUCUAACA This paper N/A Primer: GAPDH Forward: TGACTTCAACAGCGACA CCCA This paper N/A Primer: RACK1 Forward: TGAGCAGATGACCCTT CGTG This paper N/A Primer: TRIM21 Forward: GTCCTGGAAAGGAGTGA GTCC This paper N/A Primer: FPN1 Forward: CTACTTGGGGAGATCGGA TGT This paper N/A Primer: FTH1 Forward: TGAAGCTGCAGAACCA ACGAGG This paper N/A Primer: DMT1 Forward: AGCTCCACCATGACAG GAACCT This paper N/A Primer: TFRC Forward: ATCGGTTGGTGCCACT GAATGG This paper N/A Recombinant DNA pCDNA3.1-RACK1-Myc-HisA This paper N/A pCDNA3.1-RACK1 K280R-Myc-HisA This paper N/A PCDNA3.1- Myc-HisA TSINGKE N/A pCMV-TRIM21-FLAG-C This paper N/A pCMV- FLAG-C TSINGKE N/A Software and algorithms R Yu et al.44 https://www.r-project.org/ GSEA analysis Zhou et al.45 https://www.gsea-msigdb.org/ gsea/index.jsp (Continued on next page) 18 Cell Reports 44, 116563, November 25, 2025
Glutathione Assay:Article Title: The xCT/CD98 complex suppresses ferroptosis in pan-cancer via a non-canonical RACK1-mediated iron homeostasis pathway.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-RACK1 Santa Cruz Biotechnology Cat# sc-17754; RRID: AB_2247471 Mouse monoclonal anti-CD98 Santa Cruz Biotechnology Cat# sc-376815; RRID: AB_2938854 Rabbit polyclonal anti-CD98 Proteintech Cat# 15193-1-AP; RRID: AB_2254909 Rabbit polyclonal anti-SLC40A1 Proteintech Cat# 26601-1-AP; RRID: AB_2880571 Mouse monoclonal anti- Ubiquitin Cell Signaling Technology Cat# 3936S; RRID: AB_3717987 Mouse monoclonal anti-ferritin heavy chain Santa Cruz Biotechnology Cat# sc-376594; RRID: AB_11150859 Rabbit polyclonal anti-NCOA4 Abcam Cat# ab86707; RRID: AB_1925236 Rabbit polyclonal anti-CD71 proteintech Cat# 10084-2-AP; RRID: AB_2240403 Mouse anti-GAPDH Fdbio FD0063; RRID: AB_2934268 Rabbit polyclonal anti-DMT1 proteintech Cat# 20507-1-AP; RRID: AB_10694284 Rabbit monoclonal anti-xCT/SLC7A11 Cell Signaling Technology Cat# 12691S; RRID: AB_3717988 Rabbit monoclonal anti- Glutathione Peroxidase 4 Abcam Cat# ab125066; RRID: AB_10973901 Mouse monoclonal anti-HA-Tag Santa Cruz Biotechnology Cat# sc-7392; RRID: AB_627809 Mouse monoclonal anti-Flag Tag Bioss Cat# bsm-33346M; RRID: AB_3083063 Rabbit polyclonal anti-MYC-tag proteintech Cat# 16286-1-AP; RRID: AB_11182162 Mouse monoclonal anti-Myc-tag OriGene Cat# TA150121; RRID: AB_2622266 Biological samples Breast tumor and paracancerous tissue chip Sir Run Run Shaw Hospital N/A Chemicals, peptides, and recombinant proteins Sorafenib CSNpharm CSN10381 Erastin MCE HY-15763 Tamoxifen MCE HY-13757A trifluoroacetic acid Sigma 76-05-1 Ferrostatin-1 Selleck S7243 Deferoxamine MCE HY-B1625 MG132 Selleck S2619 cycloheximide Selleck S7418 chloroquine Selleck S6999 Doxorubicin Sigma D1515, Harringtonolide MACKIN H962769 Critical commercial assays PTMScan phosphotyrosine Rabbit mAb (P-Tyr-1000) Kit Cell Signaling Technology 8803 PTMScan Ubiquitin Remnant Motif (K-ε-GG) Kit Cell Signaling Technology 5562 PTMScan Acetyl-Lysine Motif [Ac-K] Kit Cell Signaling Technology 13416 protein A/G Santa Cruz Biotechnology sc-2003 RNAfast1000 fastagen 220011 RT Master Mix Takara RR036A TB Green® Premix Ex TaqTM Takara RR420 Cell Counting Kit-8 APEXBT K1018 FITC-transferrin Rockland Immunochemicals 009–0234 BODIPY 581/591 C11 ThermoFisher D3861 FerroOrange Dojindo F374 Glutathione Assay Kit Beyotime S0052 Lipofectamine ThermoFisher 56532 (Continued on next page) Cell Reports 44, 116563, November 25, 2025 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Neofect NEOFECT TF20121201 Iron Assay Kit DOJINDO I291 In Situ Red Starter Kit Mouse/Rabbit Duolink® DUO92101-1KT Deposited data The mass spectrometry proteomics data of Sorafinib Zhu et al.15 PXD045576 Experimental models: Cell lines Human: MDA-MB-231 cell line Chinese Academy of Sciences N/A Human: BT549 cell line Chinese Academy of Sciences N/A Human: HS578T cell line Chinese Academy of Sciences N/A Human: MCF7 cell line Chinese Academy of Sciences N/A Human: MHCC97-L MeisenCTCC CTCC-400-0194 Human: Panc01 cell line Chinese Academy of Sciences N/A Human: PC-3 MeisenCTCC CTCC-0337 Human: A2780 MeisenCTCC CTCC-0402 Human: A549 MeisenCTCC CTCC-0336 Human: MG803 cell line Chinese Academy of Sciences N/A Experimental models: Organisms/strains Mouse: 4-week-old female nude mice Hangzhou Medical College N/A Oligonucleotides siRNA targeting sequence: RACK1 #1: GUCUCUGGA UCUCGAGAUA; #2: GGAUGAGACCAACUAUGGA This paper N/A siRNA targeting sequence: TRIM21 #1: GCAUGGUC UCCUUCUACAA; #2: CGCAGAGUUUG UGCAGCAA This paper N/A siRNA targeting sequence: FPN1 #1: GGUGUGAAAG ACUCUAACA This paper N/A Primer: GAPDH Forward: TGACTTCAACAGCGACA CCCA This paper N/A Primer: RACK1 Forward: TGAGCAGATGACCCTT CGTG This paper N/A Primer: TRIM21 Forward: GTCCTGGAAAGGAGTGA GTCC This paper N/A Primer: FPN1 Forward: CTACTTGGGGAGATCGGA TGT This paper N/A Primer: FTH1 Forward: TGAAGCTGCAGAACCA ACGAGG This paper N/A Primer: DMT1 Forward: AGCTCCACCATGACAG GAACCT This paper N/A Primer: TFRC Forward: ATCGGTTGGTGCCACT GAATGG This paper N/A Recombinant DNA pCDNA3.1-RACK1-Myc-HisA This paper N/A pCDNA3.1-RACK1 K280R-Myc-HisA This paper N/A PCDNA3.1- Myc-HisA TSINGKE N/A pCMV-TRIM21-FLAG-C This paper N/A pCMV- FLAG-C TSINGKE N/A Software and algorithms R Yu et al.44 https://www.r-project.org/ GSEA analysis Zhou et al.45 https://www.gsea-msigdb.org/ gsea/index.jsp (Continued on next page) 18 Cell Reports 44, 116563, November 25, 2025
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