t84 ccl 248  (ATCC)

Journal: bioRxiv

Article Title: Lysosomal Alkalinization Selects for Metabolically Plastic, Motile Cancer Cells under Nutrient Stress

doi: 10.64898/2026.04.16.718649

Figure Lengend Snippet: A. Time-course viability curves of Caco-2, T84, and RKO cells were modeled using both logistic (solid lines) and exponential (dashed lines) fits at 24, 48, 72, and 96 hours. Panels represent (top) NR+Baf, (middle) ND+Baf, and (bottom) ND conditions. B. Phalloidin staining showing a remarkable increase in elongation in the ND+Baf Caco-2 cells (***p<0.001, *p<0.05, ANOVA). C . Phalloidin staining in RKO cells showing a significant increase in the elongation of ND cells as well as ND+Baf cells (****p<0.0001, **p<0.01, ANOVA). D . Phalloidin staining in T84 cells showing no change in the elongation of ND cells and an increase in the ND+Baf cells (**p<0.01, ANOVA). n=25 for elongation analyses. Scale bar: 50µm.

Article Snippet: Caco-2 cells were purchased from ŞAP Enstitüsü (Ankara, Turkey), RKO and T84 cells were purchased from ATCC.

Techniques: Staining

Journal: Frontiers in Immunology

Article Title: IFNγ-induced antigen loss in chimeric antigen receptor-T cell therapy

doi: 10.3389/fimmu.2026.1772472

Figure Lengend Snippet: GucyCART has limited efficacy in low antigen models. (A, B) GUCY2C RNA (A) and protein (B) levels in various CRC models relative to the T84 cell line. ** p < 0.01, *** p < 0.001, **** p < 0.0001, One-way ANOVA comparing each cell line to T84 cells. Each data point in (A, B) represents the average from a biological replicate. (C) Tumor burden of LS174T (N = 7) or LoVo (N = 5) cells injected i.p. in NSG mice before i.v. treatment with 5x10 6 control or GucyCART cells. Areas under the curve (AUCs) were quantified, and an unpaired T-test was used for comparisons (ns = p > 0.05). Figure schematics were generated using BioRender.com .

Article Snippet: LS174T (CL-188, ATCC), SKCO1 (HTB-39), LoVo (CCL229, ATCC), and T84 (CCL-248, ATCC) were obtained ATCC.

Techniques: Injection, Control, Generated

Journal: Frontiers in Immunology

Article Title: IFNγ-induced antigen loss in chimeric antigen receptor-T cell therapy

doi: 10.3389/fimmu.2026.1772472

Figure Lengend Snippet: CART-conditioned media induce GUCY2C loss in low antigen CRC cells. (A–F ) GucyCART cells were activated by 48 hour co-culture with T84 CRC cells (A–C) or untransduced T cells were activated with anti-CD3/CD2/CD28 beads (D–F) . Conditioned media (CM) were then collected, and fresh LS174T, SKCO1, or LoVo cells were exposed to CM for 48 hours before quantification of GUCY2C protein (B, E) and mRNA (C, F) . Controls include control CART cells (A–C) or beads lacking anti-CD3/CD2/CD28 antibodies (D-F) . Each data point represents the average of biological replicates in separate experiments (N = 3–5 experiments). A paired T-test was used to compare the conditions; ns = p > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001. Figure schematics were generated using BioRender.com .

Article Snippet: LS174T (CL-188, ATCC), SKCO1 (HTB-39), LoVo (CCL229, ATCC), and T84 (CCL-248, ATCC) were obtained ATCC.

Techniques: Co-Culture Assay, Control, Generated

Journal: Frontiers in Immunology

Article Title: IFNγ-induced antigen loss in chimeric antigen receptor-T cell therapy

doi: 10.3389/fimmu.2026.1772472

Figure Lengend Snippet: IFNγ-induced GUCY2C loss is mediated by cellular stress signaling. (A) Gene Set Enrichment Analysis (GSEA) of significantly up- and down-regulated Hallmark pathways in RNAseq data from LS174T cells treated with conditioned media (CM) from T84 co-cultures with control or GucyCART. (B) Significantly upregulated stress pathway-related genes from (A) . (C, D) LS174T cells were treated for 48 hours with CM ± 13 μg/mL anti-IFNγ neutralizing antibody (αIFNγ, C ) or 2.5 μM ruxolitinib (Ruxo, D ) and CHOP protein was quantified. (E) LS174T cells were treated with control or 2 μg/mL tunicamycin for 48 hours, and CHOP and GUCY2C proteins were quantified. Each data point in (C–E) represents the average from biological replicates in separate experiments (N = 3-4). In (E) , CHOP and GUCY2C were examined on the same blot and normalized to the GAPDH control (shown only below GUCY2C). One-way ANOVA was used to compare each condition in (C) and (D) , and a paired T-test was used to compare conditions in (E) ; ns = p > 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. Figure schematics were generated using BioRender.com .

Article Snippet: LS174T (CL-188, ATCC), SKCO1 (HTB-39), LoVo (CCL229, ATCC), and T84 (CCL-248, ATCC) were obtained ATCC.

Techniques: RNA sequencing, Control, Generated

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Silver nanoparticles at sub-cytotoxic levels increase enteric pathogen invasion by compromising intestinal epithelial barrier integrity

doi: 10.3389/fcimb.2026.1745955

Figure Lengend Snippet: Effect of 10 and 20 µg/mL AgNPs pretreatment on bacterial adhesion, invasion, and persistence in infected T84 epithelial cells. T84 cells were pretreated or 24 hours with 10 nm AgNPs at 10 or 20 µg/mL, followed infection with Salmonella enterica serovar Heidelberg strain 146. (a) Bacterial adhesion, invasion, and persistence were quantified by measuring CFU/mL (as described in M&Ms). (b) Bacterial survival percentage in T84 cells during adhesion, invasion and persistence following AgNPs treatment. Survival was calculated by dividing CFU/mL (as described in M&Ms) for each condition by its corresponding control (set to 100% and therefore not shown as a separate bar) and expressed as a percentage. Bars represent mean ± SD from three independent experiments performed in biological triplicates. Asterisks indicate statistically significant differences compared to respective untreated infection controls (** p < 0.005, *** p < 0.0005; unpaired t-test).

Article Snippet: Human colon carcinoma T84 epithelial cells (ATCC ® CCL-248TM) were cultured in complete medium composed of Dulbecco’s Modified Eagle Medium/Ham’s F-12 (DMEM/F-12) supplemented with l-glutamine and HEPES (ATCC, Manassas, VA, USA), 10% fetal bovine serum (FBS), 1% penicillin-streptomycin and 0.1% fungizone (Thermo Fisher Scientific, Waltham, MA, USA).

Techniques: Infection, Control

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Silver nanoparticles at sub-cytotoxic levels increase enteric pathogen invasion by compromising intestinal epithelial barrier integrity

doi: 10.3389/fcimb.2026.1745955

Figure Lengend Snippet: Relative expression of genes related to cell-cell junction and epithelial barrier function in T84 cells following AgNPs exposure and bacterial infection. T84 cells were pretreated with 10 nm AgNPs at either 10 µg/mL or 20 µg/mL, alone or in combination with S. enterica under invasion (1 h + gentamicin) or persistence (24 h + gentamicin) conditions. Invasion control consists of infected cells that are not pretreated with AgNPs but treated with gentamicin for 1 hour, while persistence control consists of infected cells not pretreated with AgNPs but treated with gentamicin for 24 hours. Gene expression (as described in M&Ms) was measured using RT² Profiler PCR arrays and is represented as Log 2 fold regulation relative to untreated control cells. (a) Focal adhesion genes, (b) Gap junction genes, (c) Tight junction genes, and (d) Adherens Junctions, desmosomes, and hemidesmosomes genes. Bars represent mean ± SE from biological triplicates. Asterisks indicate statistically significant differences compared to uninfected and AgNPs untreated control (p < 0.05) (*p < 0.05, **p < 0.005; unpaired t-test).

Article Snippet: Human colon carcinoma T84 epithelial cells (ATCC ® CCL-248TM) were cultured in complete medium composed of Dulbecco’s Modified Eagle Medium/Ham’s F-12 (DMEM/F-12) supplemented with l-glutamine and HEPES (ATCC, Manassas, VA, USA), 10% fetal bovine serum (FBS), 1% penicillin-streptomycin and 0.1% fungizone (Thermo Fisher Scientific, Waltham, MA, USA).

Techniques: Expressing, Infection, Control, Gene Expression

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Silver nanoparticles at sub-cytotoxic levels increase enteric pathogen invasion by compromising intestinal epithelial barrier integrity

doi: 10.3389/fcimb.2026.1745955

Figure Lengend Snippet: Concentration of pro- and anti-inflammatory cytokines in T84 epithelial cells following AgNPs exposure and bacterial infection. Cytokine concentrations in the supernatants of T84 cells pretreated with 10 µg/mL or 20 µg/mL of 10 nm AgNPs and exposed to S. enterica under invasion or persistence conditions. (a) Pro-inflammatory cytokines and (b) Anti-inflammatory cytokine. Bars represent mean ± SE from biological triplicates. Asterisks indicate statistically significant differences compared to uninfected and AgNPs untreated control (p < 0.05).

Article Snippet: Human colon carcinoma T84 epithelial cells (ATCC ® CCL-248TM) were cultured in complete medium composed of Dulbecco’s Modified Eagle Medium/Ham’s F-12 (DMEM/F-12) supplemented with l-glutamine and HEPES (ATCC, Manassas, VA, USA), 10% fetal bovine serum (FBS), 1% penicillin-streptomycin and 0.1% fungizone (Thermo Fisher Scientific, Waltham, MA, USA).

Techniques: Concentration Assay, Infection, Control

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Silver nanoparticles at sub-cytotoxic levels increase enteric pathogen invasion by compromising intestinal epithelial barrier integrity

doi: 10.3389/fcimb.2026.1745955

Figure Lengend Snippet: Concentration of growth factors, chemokines, and Th2 cytokines in T84 epithelial cells following AgNPs exposure and bacterial infection. T84 cells were treated with 10 nm AgNPs at 10 µg/mL or 20 µg/mL, either alone or in combination with S. enterica invasion or persistence. Invasion control consisted of infected cells that were not pretreated with AgNPs but treated with gentamicin for 1 hour, while persistence control consisted of infected cells not pretreated with AgNPs but treated with gentamicin for 24 hours. (a) Growth factors, (b) chemokines, and (c) Th2 cytokines. Bars represent mean ± SE from biological triplicates. Asterisks indicate statistically significant differences compared to control (p < 0.05).

Article Snippet: Human colon carcinoma T84 epithelial cells (ATCC ® CCL-248TM) were cultured in complete medium composed of Dulbecco’s Modified Eagle Medium/Ham’s F-12 (DMEM/F-12) supplemented with l-glutamine and HEPES (ATCC, Manassas, VA, USA), 10% fetal bovine serum (FBS), 1% penicillin-streptomycin and 0.1% fungizone (Thermo Fisher Scientific, Waltham, MA, USA).

Techniques: Concentration Assay, Infection, Control