Journal: Molecules
Article Title: Protective Effects of Chlorella -Derived Peptide Against UVC-Induced Cytotoxicity through Inhibition of Caspase-3 Activity and Reduction of the Expression of Phosphorylated FADD and Cleaved PARP-1 in Skin Fibroblasts
doi: 10.3390/molecules17089116
Figure Lengend Snippet: Inhibitory effects of CDP on UVC-induced caspase-3 activity. Skin fibroblasts were treated with CDP (10 mg/mL), vitamin C (150 μM), or vitamin E (25 μM) after UVC exposure with a total dose of 15 J/cm 2 at 12, 24, 48, and 72 h prior to caspase-3 activity assay. Statistics are shown for UVC treated group, ## p < 0.01 compared to basal group, * p < 0.05 compared to UVC group at 12 and 24 h after the UV exposure, *** p < 0.005 compared to UVC group 48 and 72 h after the UV exposure.
Article Snippet: Radioimmunoprecipitation assay buffer (R0278), vitamin C (A5960), vitamin E (T3634), PJ34 (P4365), and monoclonal anti-alpha-tubulin (T5168) were purchased from Sigma (Taipei, Taiwan).
Techniques: Activity Assay, Caspase-3 Activity Assay