Journal: Frontiers in Pharmacology
Article Title: Salidroside derivative SHPL-49 enhances synaptic remodeling in BCCAO rats via the CDK5/p35/p25 signaling pathway
doi: 10.3389/fphar.2026.1727177
Figure Lengend Snippet: SHPL-49 at a dosage of 90 mg/kg enhances synaptic plasticity. (A) Representative Golgi-Cox staining images of rat brain tissue. (B) Quantitative analysis of dendritic spine density. (C–E) qRT-PCR analysis of key markers associated with synaptic remodeling: SYP, β-III tubulin and MAP-2. (F) Representative Western blot images showing the expression of presynaptic markers SYP and SYN1, as well as the postsynaptic marker PSD95, in rat brain tissue. (G) Quantification of SYP protein levels in rat brain tissue. (H) Quantification of SYN1 protein levels in rat brain tissue. (I) Quantification of PSD95 protein levels in rat brain tissue. (J) Immunofluorescence staining image of SYP, SYN1, PSD95, and the glial scar. Scale bars = 50 μm for synaptic markers; and 200 μm for the glial scar. (K) Quantification of SYP fluorescence intensity based on immunofluorescence staining. (L) Quantification of SYN1 fluorescence intensity based on immunofluorescence staining. (M) Quantification of PSD95 fluorescence intensity based on immunofluorescence staining. (N) Quantification of glial scar area based on immunofluorescence Staining. Data are presented as mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001, Model group vs. the Sham group, # P < 0.05, ## P < 0.01, ### P < 0.001, SHPL-49 group or SAL group vs. the Model group. n = 6 per group; & P < 0.05, &&& P < 0.001, SHPL-49 group vs. SAL group.
Article Snippet: Membranes were blocked with 5% non-fat milk (Beyotime, China) in Tris-buffered saline containing 0.1% Tween-20 (TBST) and incubated overnight at 4 °C with the following primary antibodies: SYP (1:1,000, CST, United States), SYN1 (1:1,000, Boster, China), PSD95 (1:1,000, ABCAM, United States), CDK5 (1:1,000, Boster, China), p35/25 (1:1,000, CST, United States), p-PSD95 (1:1,000, CST, United States).
Techniques: Staining, Quantitative RT-PCR, Western Blot, Expressing, Marker, Immunofluorescence, Fluorescence
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