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Proteintech syp
Syp, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 65 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/syp/NSE+Antibody/pm41881959-314-23-24
Average 94 stars, based on 65 article reviews
syp - by Bioz Stars, 2026-09
94/100 stars

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Related Articles

Saline:

Article Title: Chlorpyrifos-induced autism and depression-like behaviors are ameliorated by trehalose: Integrating network pharmacology and toxicology analyses and animal experiments.
Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.

Incubation:

Article Title: Chlorpyrifos-induced autism and depression-like behaviors are ameliorated by trehalose: Integrating network pharmacology and toxicology analyses and animal experiments.
Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.

Article Title: UHRF1 regulates AR ubiquitination to promote the loss of AR signaling and enzalutamide resistance in progression of prostate cancer
Article Snippet: .. After blocking with 5% milk in PBS containing 0.1% Tween-20 for 1 h at room temperature, membranes were incubated overnight at 4 °C with primary antibodies: AR (Santa Cruz Biotechnology Inc., Dallas, TX, USA; Cat# sc-7305, 1:1000; Cell Signaling Technology, Danvers, MA, USA; Cat# 5153S, 1:1000), UHRF1 (Proteintech Group, Rosemont, IL, USA; Cat# 21402-1-AP, 1:1000), Ub (ZenBio Inc., Durham, NC, USA; Cat# 382766, 1:1000), NSE (Proteintech Group; Cat# 10149-1-AP, 1:1000), SYP (Proteintech Group; Cat# 17785-1-AP, 1:1000), Flag (Vazyme Biotech Co., Ltd., Nanjing, Jiangsu, China; Cat# RA1003-01, 1:1000), and GAPDH (Santa Cruz Biotechnology Inc.; Cat# sc-47724, 1:1000) as a loading control. ..

Article Title: UHRF1 regulates AR ubiquitination to promote the loss of AR signaling and enzalutamide resistance in progression of prostate cancer.
Article Snippet: .. After blocking with 5% milk in PBS containing 0.1% Tween-20 for 1 hour at room temperature, membranes were incubated overnight at 4°C with primary antibodies: AR (Santa Cruz Biotechnology Inc., Dallas, TX, USA; Cat# sc-7305, 1:1000; Cell Signaling Technology, Danvers, MA, USA; Cat# 5153S, 1:1000), UHRF1 (Proteintech Group, Rosemont, AR TI CL E IN P RE SS IL, USA; Cat# 21402-1-AP, 1:1000), Ub (ZenBio Inc., Durham, NC, USA; Cat# 382766, 1:1000), NSE (Proteintech Group; Cat# 10149-1-AP, 1:1000), SYP (Proteintech Group; Cat# 17785-1-AP, 1:1000), Flag (Vazyme Biotech Co., Ltd., Nanjing, Jiangsu, China; Cat# RA100301, 1:1000), and GAPDH (Santa Cruz Biotechnology Inc.; Cat# sc-47724, 1:1000) as a loading control. ..

Western Blot:

Article Title: OPRK1 drives SLC9A3R1 progression to neuroendocrine prostate cancer.
Article Snippet: .. The antibodies used for immunoblotting include GAPDH (60004-1-Ig, Proteintech), 465 OPRK1 (ab113533, abcam), AR (5153, Cell Signaling Technologies), PSA/KLK3 (5365, Cell 466 Signaling Technologies), NKX3.1 (83700, Cell Signaling Technologies), NSE (10149-1-AP, 467 Proteintech), SYP (17785-1-AP, Proteintech), HCG/CgA (25014-1-AP, Proteintech), CK8 (17514-468 1-AP, Proteintech),SLC9A3R1 (29771-1-AP), Beclin1 (11306-1-AP, Proteintech), REST (22242-469 1-AP, Proteintech), N-Cadherin (R380671, zenbio), E-Cadherin (R22490, zenbio), VIM/vimentin 470 (R22775, zenbio), LC3B (14600-1-AP, Proteintech) and P62 (18420-1-AP, Proteintech). .. Using 471 BIO-RAD GelDoc XR to detect the blot bands.

Article Title: OPRK1 drives SLC9A3R1 progression to neuroendocrine prostate cancer
Article Snippet: .. The antibodies used for immunoblotting include GAPDH (60004-1-Ig, Proteintech), OPRK1 (ab113533, abcam), AR (5153, Cell Signaling Technologies), PSA/KLK3 (5365, Cell Signaling Technologies), NKX3.1 (83700, Cell Signaling Technologies), NSE (10149-1-AP, Proteintech), SYP (17785-1-AP, Proteintech), HCG/CgA (25014-1-AP, Proteintech), CK8 (17514-1-AP, Proteintech), SLC9A3R1 (29771-1-AP), Beclin1 (11306-1-AP, Proteintech), REST (22242-1-AP, Proteintech), N-Cadherin (R380671, zenbio), E-Cadherin (R22490, zenbio), VIM/vimentin (R22775, zenbio), LC3B (14600-1-AP, Proteintech) and P62 (18420-1-AP, Proteintech). .. Using BIO-RAD GelDoc XR to detect the blot bands.

Blocking Assay:

Article Title: UHRF1 regulates AR ubiquitination to promote the loss of AR signaling and enzalutamide resistance in progression of prostate cancer
Article Snippet: .. After blocking with 5% milk in PBS containing 0.1% Tween-20 for 1 h at room temperature, membranes were incubated overnight at 4 °C with primary antibodies: AR (Santa Cruz Biotechnology Inc., Dallas, TX, USA; Cat# sc-7305, 1:1000; Cell Signaling Technology, Danvers, MA, USA; Cat# 5153S, 1:1000), UHRF1 (Proteintech Group, Rosemont, IL, USA; Cat# 21402-1-AP, 1:1000), Ub (ZenBio Inc., Durham, NC, USA; Cat# 382766, 1:1000), NSE (Proteintech Group; Cat# 10149-1-AP, 1:1000), SYP (Proteintech Group; Cat# 17785-1-AP, 1:1000), Flag (Vazyme Biotech Co., Ltd., Nanjing, Jiangsu, China; Cat# RA1003-01, 1:1000), and GAPDH (Santa Cruz Biotechnology Inc.; Cat# sc-47724, 1:1000) as a loading control. ..

Article Title: UHRF1 regulates AR ubiquitination to promote the loss of AR signaling and enzalutamide resistance in progression of prostate cancer.
Article Snippet: .. After blocking with 5% milk in PBS containing 0.1% Tween-20 for 1 hour at room temperature, membranes were incubated overnight at 4°C with primary antibodies: AR (Santa Cruz Biotechnology Inc., Dallas, TX, USA; Cat# sc-7305, 1:1000; Cell Signaling Technology, Danvers, MA, USA; Cat# 5153S, 1:1000), UHRF1 (Proteintech Group, Rosemont, AR TI CL E IN P RE SS IL, USA; Cat# 21402-1-AP, 1:1000), Ub (ZenBio Inc., Durham, NC, USA; Cat# 382766, 1:1000), NSE (Proteintech Group; Cat# 10149-1-AP, 1:1000), SYP (Proteintech Group; Cat# 17785-1-AP, 1:1000), Flag (Vazyme Biotech Co., Ltd., Nanjing, Jiangsu, China; Cat# RA100301, 1:1000), and GAPDH (Santa Cruz Biotechnology Inc.; Cat# sc-47724, 1:1000) as a loading control. ..

Control:

Article Title: UHRF1 regulates AR ubiquitination to promote the loss of AR signaling and enzalutamide resistance in progression of prostate cancer
Article Snippet: .. After blocking with 5% milk in PBS containing 0.1% Tween-20 for 1 h at room temperature, membranes were incubated overnight at 4 °C with primary antibodies: AR (Santa Cruz Biotechnology Inc., Dallas, TX, USA; Cat# sc-7305, 1:1000; Cell Signaling Technology, Danvers, MA, USA; Cat# 5153S, 1:1000), UHRF1 (Proteintech Group, Rosemont, IL, USA; Cat# 21402-1-AP, 1:1000), Ub (ZenBio Inc., Durham, NC, USA; Cat# 382766, 1:1000), NSE (Proteintech Group; Cat# 10149-1-AP, 1:1000), SYP (Proteintech Group; Cat# 17785-1-AP, 1:1000), Flag (Vazyme Biotech Co., Ltd., Nanjing, Jiangsu, China; Cat# RA1003-01, 1:1000), and GAPDH (Santa Cruz Biotechnology Inc.; Cat# sc-47724, 1:1000) as a loading control. ..

Article Title: UHRF1 regulates AR ubiquitination to promote the loss of AR signaling and enzalutamide resistance in progression of prostate cancer.
Article Snippet: .. After blocking with 5% milk in PBS containing 0.1% Tween-20 for 1 hour at room temperature, membranes were incubated overnight at 4°C with primary antibodies: AR (Santa Cruz Biotechnology Inc., Dallas, TX, USA; Cat# sc-7305, 1:1000; Cell Signaling Technology, Danvers, MA, USA; Cat# 5153S, 1:1000), UHRF1 (Proteintech Group, Rosemont, AR TI CL E IN P RE SS IL, USA; Cat# 21402-1-AP, 1:1000), Ub (ZenBio Inc., Durham, NC, USA; Cat# 382766, 1:1000), NSE (Proteintech Group; Cat# 10149-1-AP, 1:1000), SYP (Proteintech Group; Cat# 17785-1-AP, 1:1000), Flag (Vazyme Biotech Co., Ltd., Nanjing, Jiangsu, China; Cat# RA100301, 1:1000), and GAPDH (Santa Cruz Biotechnology Inc.; Cat# sc-47724, 1:1000) as a loading control. ..



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SHPL-49 at a dosage of 90 mg/kg enhances synaptic plasticity. (A) Representative Golgi-Cox staining images of rat brain tissue. (B) Quantitative analysis of dendritic spine density. (C–E) qRT-PCR analysis of key markers associated with synaptic remodeling: <t>SYP,</t> β-III tubulin and MAP-2. (F) Representative Western blot images showing the expression of presynaptic markers SYP <t>and</t> <t>SYN1,</t> as well as the postsynaptic marker PSD95, in rat brain tissue. (G) Quantification of SYP protein levels in rat brain tissue. (H) Quantification of SYN1 protein levels in rat brain tissue. (I) Quantification of PSD95 protein levels in rat brain tissue. (J) Immunofluorescence staining image of SYP, SYN1, PSD95, and the glial scar. Scale bars = 50 μm for synaptic markers; and 200 μm for the glial scar. (K) Quantification of SYP fluorescence intensity based on immunofluorescence staining. (L) Quantification of SYN1 fluorescence intensity based on immunofluorescence staining. (M) Quantification of PSD95 fluorescence intensity based on immunofluorescence staining. (N) Quantification of glial scar area based on immunofluorescence Staining. Data are presented as mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001, Model group vs. the Sham group, # P < 0.05, ## P < 0.01, ### P < 0.001, SHPL-49 group or SAL group vs. the Model group. n = 6 per group; & P < 0.05, &&& P < 0.001, SHPL-49 group vs. SAL group.
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Image Search Results


The effect of ozone exposure on tumor cells was studied by measuring ROS levels in B16F10 melanoma and LL2 lung carcinoma cells. (A) The ROS content was assessed using the ROS-ID Total ROS Detection Kit. Data from the 520 nm fluorescence indicates increased ROS levels. The increase in ROS in (B) B16F10 melanoma and (C) LL2 lung carcinoma cells was observed after three days of treatment with air or ozone, with or without arbutin (1.56 µM). These measurements were performed using the Chekine TM reactive oxygen species detection fluorometric assay. (D) Cell proliferation of B16F10 melanoma and LL2 lung carcinoma was evaluated following three-day ozone exposure, revealing enhanced proliferation in both cell lines. Results are shown as mean ± SD with a sample size of 6; statistical significance is indicated by *P < 0.05, **P < 0.01, and ***P < 0.001. Western blot images display the expression of PCNA and HIF-1α proteins in (E) B16F10 (F) LL2 cells exposed to ozone or air at various time points.

Journal: American Journal of Cancer Research

Article Title: Reactive oxygen species-dependent regulation of hypoxia-inducible factor 1α/C-X-C motif chemokine receptor 4 signaling promotes ozone-induced cancer metastasis

doi: 10.62347/DKDS6537

Figure Lengend Snippet: The effect of ozone exposure on tumor cells was studied by measuring ROS levels in B16F10 melanoma and LL2 lung carcinoma cells. (A) The ROS content was assessed using the ROS-ID Total ROS Detection Kit. Data from the 520 nm fluorescence indicates increased ROS levels. The increase in ROS in (B) B16F10 melanoma and (C) LL2 lung carcinoma cells was observed after three days of treatment with air or ozone, with or without arbutin (1.56 µM). These measurements were performed using the Chekine TM reactive oxygen species detection fluorometric assay. (D) Cell proliferation of B16F10 melanoma and LL2 lung carcinoma was evaluated following three-day ozone exposure, revealing enhanced proliferation in both cell lines. Results are shown as mean ± SD with a sample size of 6; statistical significance is indicated by *P < 0.05, **P < 0.01, and ***P < 0.001. Western blot images display the expression of PCNA and HIF-1α proteins in (E) B16F10 (F) LL2 cells exposed to ozone or air at various time points.

Article Snippet: The HIF-1α inhibitor (SYP-5) was purchased from MedChemExpress (MedChemExpress LLC, Monmouth Junction, NJ, USA).

Techniques: Fluorescence, Western Blot, Expressing

The effects of ozone on the HIF-1α/CXCR4 pathway and EMT markers in B16F10 and LL2 in vitro models. Western blot images showing levels of HIF-1α, CXCR4, E-cadherin, N-cadherin, MMP-2, and SNAIL proteins. Inset values represent protein expression levels normalized to β-Actin.

Journal: American Journal of Cancer Research

Article Title: Reactive oxygen species-dependent regulation of hypoxia-inducible factor 1α/C-X-C motif chemokine receptor 4 signaling promotes ozone-induced cancer metastasis

doi: 10.62347/DKDS6537

Figure Lengend Snippet: The effects of ozone on the HIF-1α/CXCR4 pathway and EMT markers in B16F10 and LL2 in vitro models. Western blot images showing levels of HIF-1α, CXCR4, E-cadherin, N-cadherin, MMP-2, and SNAIL proteins. Inset values represent protein expression levels normalized to β-Actin.

Article Snippet: The HIF-1α inhibitor (SYP-5) was purchased from MedChemExpress (MedChemExpress LLC, Monmouth Junction, NJ, USA).

Techniques: In Vitro, Western Blot, Expressing

The ROS-related effects of in vitro arbutin application on ozone-exposed EMT depolarization and HIF-1α/CXCR4 pathway downregulation in (A) B16F10 and (B) LL2 cells. Protein expressions of HIF-1α, CXCR4, E-cadherin (E-Cad), N-cadherin (N-Cad), MMP-2, and SNAIL are shown with arbutin-reversal treatment. Inset values indicate protein levels normalized to β-Actin. Each experiment was repeated three times with similar results.

Journal: American Journal of Cancer Research

Article Title: Reactive oxygen species-dependent regulation of hypoxia-inducible factor 1α/C-X-C motif chemokine receptor 4 signaling promotes ozone-induced cancer metastasis

doi: 10.62347/DKDS6537

Figure Lengend Snippet: The ROS-related effects of in vitro arbutin application on ozone-exposed EMT depolarization and HIF-1α/CXCR4 pathway downregulation in (A) B16F10 and (B) LL2 cells. Protein expressions of HIF-1α, CXCR4, E-cadherin (E-Cad), N-cadherin (N-Cad), MMP-2, and SNAIL are shown with arbutin-reversal treatment. Inset values indicate protein levels normalized to β-Actin. Each experiment was repeated three times with similar results.

Article Snippet: The HIF-1α inhibitor (SYP-5) was purchased from MedChemExpress (MedChemExpress LLC, Monmouth Junction, NJ, USA).

Techniques: In Vitro

SHPL-49 at a dosage of 90 mg/kg enhances synaptic plasticity. (A) Representative Golgi-Cox staining images of rat brain tissue. (B) Quantitative analysis of dendritic spine density. (C–E) qRT-PCR analysis of key markers associated with synaptic remodeling: SYP, β-III tubulin and MAP-2. (F) Representative Western blot images showing the expression of presynaptic markers SYP and SYN1, as well as the postsynaptic marker PSD95, in rat brain tissue. (G) Quantification of SYP protein levels in rat brain tissue. (H) Quantification of SYN1 protein levels in rat brain tissue. (I) Quantification of PSD95 protein levels in rat brain tissue. (J) Immunofluorescence staining image of SYP, SYN1, PSD95, and the glial scar. Scale bars = 50 μm for synaptic markers; and 200 μm for the glial scar. (K) Quantification of SYP fluorescence intensity based on immunofluorescence staining. (L) Quantification of SYN1 fluorescence intensity based on immunofluorescence staining. (M) Quantification of PSD95 fluorescence intensity based on immunofluorescence staining. (N) Quantification of glial scar area based on immunofluorescence Staining. Data are presented as mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001, Model group vs. the Sham group, # P < 0.05, ## P < 0.01, ### P < 0.001, SHPL-49 group or SAL group vs. the Model group. n = 6 per group; & P < 0.05, &&& P < 0.001, SHPL-49 group vs. SAL group.

Journal: Frontiers in Pharmacology

Article Title: Salidroside derivative SHPL-49 enhances synaptic remodeling in BCCAO rats via the CDK5/p35/p25 signaling pathway

doi: 10.3389/fphar.2026.1727177

Figure Lengend Snippet: SHPL-49 at a dosage of 90 mg/kg enhances synaptic plasticity. (A) Representative Golgi-Cox staining images of rat brain tissue. (B) Quantitative analysis of dendritic spine density. (C–E) qRT-PCR analysis of key markers associated with synaptic remodeling: SYP, β-III tubulin and MAP-2. (F) Representative Western blot images showing the expression of presynaptic markers SYP and SYN1, as well as the postsynaptic marker PSD95, in rat brain tissue. (G) Quantification of SYP protein levels in rat brain tissue. (H) Quantification of SYN1 protein levels in rat brain tissue. (I) Quantification of PSD95 protein levels in rat brain tissue. (J) Immunofluorescence staining image of SYP, SYN1, PSD95, and the glial scar. Scale bars = 50 μm for synaptic markers; and 200 μm for the glial scar. (K) Quantification of SYP fluorescence intensity based on immunofluorescence staining. (L) Quantification of SYN1 fluorescence intensity based on immunofluorescence staining. (M) Quantification of PSD95 fluorescence intensity based on immunofluorescence staining. (N) Quantification of glial scar area based on immunofluorescence Staining. Data are presented as mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001, Model group vs. the Sham group, # P < 0.05, ## P < 0.01, ### P < 0.001, SHPL-49 group or SAL group vs. the Model group. n = 6 per group; & P < 0.05, &&& P < 0.001, SHPL-49 group vs. SAL group.

Article Snippet: Membranes were blocked with 5% non-fat milk (Beyotime, China) in Tris-buffered saline containing 0.1% Tween-20 (TBST) and incubated overnight at 4 °C with the following primary antibodies: SYP (1:1,000, CST, United States), SYN1 (1:1,000, Boster, China), PSD95 (1:1,000, ABCAM, United States), CDK5 (1:1,000, Boster, China), p35/25 (1:1,000, CST, United States), p-PSD95 (1:1,000, CST, United States).

Techniques: Staining, Quantitative RT-PCR, Western Blot, Expressing, Marker, Immunofluorescence, Fluorescence