Review



synaptopodin  (Proteintech)


Bioz Verified Symbol Proteintech is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 94
      Buy from Supplier

    Structured Review

    Proteintech synaptopodin
    Podocyte-specific TLR4 deletion mitigated STZ-induced DKD, podocyte injury, and proteinuria in mice. (A) Schematic description of the strategy for generating STZ-induced diabetic mouse model in NPHS2 -Cre; TLR4 fl/fl ( TLR4 CKO) mice and in TLR4 fl/fl mice (CTL). (B) Monthly monitoring of plasma glucose levels in mice. Data are presented as mean ± SD. ** P < 0.01 vs. CTL-NC; ## P < 0.01 vs. CKO-NC. (C) Monthly monitoring of body weight in mice. Data are presented as mean ± SD. ** P < 0.01 vs. CTL-NC; # P < 0.05 vs. CKO-NC; # # P < 0.01 vs. CKO-NC; # ### P < 0.01 vs. CTL-STZ. (D) Representative images of PAS and MASSON staining of the glomeruli in mice at 12 and 16 weeks. Scale bar, 20 μm ( n = 8). (E) Representative images of HE staining of the kidney in mice at 12 and 16 weeks. Scale bar, 50 μm ( n = 8). (F) Representative IF images of the expression of SD proteins p-cadherin and <t>synaptopodin</t> in glomeruli at 12 and 16 weeks. Scale bar, 20 μm ( n = 8). (G) The expression of a mesenchymal marker desmin was detected in renal tissues from mice at 12 and 16 weeks by western blot analysis. P values were determined by one-way ANOVA and data are presented as mean ± SD. **** P < 0.01; # P < 0.05; ## P < 0.05 ( n = 8). (H) Representative images of TEM in podocyte ultrastructural alterations in mice. FPs effacement (blue arrow), ER dilation (red arrow) and GBM thickness were observed in podocytes in STZ-induced CTL diabetic mice, and were attenuated in TLR4 CKO diabetic mice at 16 weeks. Scale bar, 1 μm ( n = 8). (I) Determination of proteinuria in mice. Data are presented as mean ± SD. ** P < 0.01 vs. CTL-NC; # P < 0.05 vs. CKO-NC; # # P < 0.01 vs. CKO-NC; # ## P < 0.05 vs. CTL-STZ; # ### P < 0.01 vs. CTL-STZ.
    Synaptopodin, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 88 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/synaptopodin/product/Proteintech
    Average 94 stars, based on 88 article reviews
    synaptopodin - by Bioz Stars, 2026-06
    94/100 stars

    Images

    1) Product Images from "Podocyte TLR4 deletion alleviates diabetic kidney disease through prohibiting PKCδ/SHP-1-dependent ER stress and relieving podocyte damage and inflammation"

    Article Title: Podocyte TLR4 deletion alleviates diabetic kidney disease through prohibiting PKCδ/SHP-1-dependent ER stress and relieving podocyte damage and inflammation

    Journal: Journal of Advanced Research

    doi: 10.1016/j.jare.2025.07.013

    Podocyte-specific TLR4 deletion mitigated STZ-induced DKD, podocyte injury, and proteinuria in mice. (A) Schematic description of the strategy for generating STZ-induced diabetic mouse model in NPHS2 -Cre; TLR4 fl/fl ( TLR4 CKO) mice and in TLR4 fl/fl mice (CTL). (B) Monthly monitoring of plasma glucose levels in mice. Data are presented as mean ± SD. ** P < 0.01 vs. CTL-NC; ## P < 0.01 vs. CKO-NC. (C) Monthly monitoring of body weight in mice. Data are presented as mean ± SD. ** P < 0.01 vs. CTL-NC; # P < 0.05 vs. CKO-NC; # # P < 0.01 vs. CKO-NC; # ### P < 0.01 vs. CTL-STZ. (D) Representative images of PAS and MASSON staining of the glomeruli in mice at 12 and 16 weeks. Scale bar, 20 μm ( n = 8). (E) Representative images of HE staining of the kidney in mice at 12 and 16 weeks. Scale bar, 50 μm ( n = 8). (F) Representative IF images of the expression of SD proteins p-cadherin and synaptopodin in glomeruli at 12 and 16 weeks. Scale bar, 20 μm ( n = 8). (G) The expression of a mesenchymal marker desmin was detected in renal tissues from mice at 12 and 16 weeks by western blot analysis. P values were determined by one-way ANOVA and data are presented as mean ± SD. **** P < 0.01; # P < 0.05; ## P < 0.05 ( n = 8). (H) Representative images of TEM in podocyte ultrastructural alterations in mice. FPs effacement (blue arrow), ER dilation (red arrow) and GBM thickness were observed in podocytes in STZ-induced CTL diabetic mice, and were attenuated in TLR4 CKO diabetic mice at 16 weeks. Scale bar, 1 μm ( n = 8). (I) Determination of proteinuria in mice. Data are presented as mean ± SD. ** P < 0.01 vs. CTL-NC; # P < 0.05 vs. CKO-NC; # # P < 0.01 vs. CKO-NC; # ## P < 0.05 vs. CTL-STZ; # ### P < 0.01 vs. CTL-STZ.
    Figure Legend Snippet: Podocyte-specific TLR4 deletion mitigated STZ-induced DKD, podocyte injury, and proteinuria in mice. (A) Schematic description of the strategy for generating STZ-induced diabetic mouse model in NPHS2 -Cre; TLR4 fl/fl ( TLR4 CKO) mice and in TLR4 fl/fl mice (CTL). (B) Monthly monitoring of plasma glucose levels in mice. Data are presented as mean ± SD. ** P < 0.01 vs. CTL-NC; ## P < 0.01 vs. CKO-NC. (C) Monthly monitoring of body weight in mice. Data are presented as mean ± SD. ** P < 0.01 vs. CTL-NC; # P < 0.05 vs. CKO-NC; # # P < 0.01 vs. CKO-NC; # ### P < 0.01 vs. CTL-STZ. (D) Representative images of PAS and MASSON staining of the glomeruli in mice at 12 and 16 weeks. Scale bar, 20 μm ( n = 8). (E) Representative images of HE staining of the kidney in mice at 12 and 16 weeks. Scale bar, 50 μm ( n = 8). (F) Representative IF images of the expression of SD proteins p-cadherin and synaptopodin in glomeruli at 12 and 16 weeks. Scale bar, 20 μm ( n = 8). (G) The expression of a mesenchymal marker desmin was detected in renal tissues from mice at 12 and 16 weeks by western blot analysis. P values were determined by one-way ANOVA and data are presented as mean ± SD. **** P < 0.01; # P < 0.05; ## P < 0.05 ( n = 8). (H) Representative images of TEM in podocyte ultrastructural alterations in mice. FPs effacement (blue arrow), ER dilation (red arrow) and GBM thickness were observed in podocytes in STZ-induced CTL diabetic mice, and were attenuated in TLR4 CKO diabetic mice at 16 weeks. Scale bar, 1 μm ( n = 8). (I) Determination of proteinuria in mice. Data are presented as mean ± SD. ** P < 0.01 vs. CTL-NC; # P < 0.05 vs. CKO-NC; # # P < 0.01 vs. CKO-NC; # ## P < 0.05 vs. CTL-STZ; # ### P < 0.01 vs. CTL-STZ.

    Techniques Used: Clinical Proteomics, Staining, Expressing, Marker, Western Blot

    Activation of TLR4/MyD88/PKCδ/SHP-1 signaling cascade was involved in hyperglycemic podocyte damage in vivo and in vitro . (A) The protein levels of TLR4 were determined in renal tissues from mice at 12 and 16 weeks by western blot analysis. P values were determined by one-way ANOVA and data are presented as mean ± SD. **** P < 0.01; # P < 0.05 ( n = 8). (B) Representative western blot of the expression of MyD88, p-PKCδ (Y311), PKCδ and SHP-1 in renal cortex from mice at 12 and 16 weeks. P values were determined by one-way ANOVA and data are presented as mean ± SD. **** P < 0.01; # P < 0.05; ## P < 0.01; ### P < 0.01 ( n = 8). (C) Representative double IF staining of glomerular SHP-1 and synaptopodin expression in mice at 16 weeks. Scale bar, 20 μm ( n = 8). (D) The expression of TLR4, MyD88, p-PKCδ, PKCδ and SHP-1 in cultured mouse podocytes from treatments of LG or HG for 48 h with or without transfection of TLR4, MyD88 or scrambled shRNAs. P values were determined by one-way ANOVA and data are presented as mean ± SD. **** P < 0.01; ## P < 0.01; ### P < 0.01; #### P < 0.01 ( n = 3 independent experiments). (E) IF staining of podocyte proteins p-cadherin and synaptopodin after LG or HG stimulation for 48 h with or without transfection of TLR4, PKCδ, MCP-1 or scrambled shRNAs, or pretreatment of 4-PBA. Magnification 400 × . ( n = 3 independent experiments). (F) Determination of SD protein podocin and injury marker desmin in podocytes treated with LG or HG for 48 h with or without transfection of TLR4, MyD88 or scrambled shRNAs. P values were determined by one-way ANOVA and data are presented as mean ± SD. **** P < 0.01; ### P < 0.01; #### P < 0.01 ( n = 3 independent experiments).
    Figure Legend Snippet: Activation of TLR4/MyD88/PKCδ/SHP-1 signaling cascade was involved in hyperglycemic podocyte damage in vivo and in vitro . (A) The protein levels of TLR4 were determined in renal tissues from mice at 12 and 16 weeks by western blot analysis. P values were determined by one-way ANOVA and data are presented as mean ± SD. **** P < 0.01; # P < 0.05 ( n = 8). (B) Representative western blot of the expression of MyD88, p-PKCδ (Y311), PKCδ and SHP-1 in renal cortex from mice at 12 and 16 weeks. P values were determined by one-way ANOVA and data are presented as mean ± SD. **** P < 0.01; # P < 0.05; ## P < 0.01; ### P < 0.01 ( n = 8). (C) Representative double IF staining of glomerular SHP-1 and synaptopodin expression in mice at 16 weeks. Scale bar, 20 μm ( n = 8). (D) The expression of TLR4, MyD88, p-PKCδ, PKCδ and SHP-1 in cultured mouse podocytes from treatments of LG or HG for 48 h with or without transfection of TLR4, MyD88 or scrambled shRNAs. P values were determined by one-way ANOVA and data are presented as mean ± SD. **** P < 0.01; ## P < 0.01; ### P < 0.01; #### P < 0.01 ( n = 3 independent experiments). (E) IF staining of podocyte proteins p-cadherin and synaptopodin after LG or HG stimulation for 48 h with or without transfection of TLR4, PKCδ, MCP-1 or scrambled shRNAs, or pretreatment of 4-PBA. Magnification 400 × . ( n = 3 independent experiments). (F) Determination of SD protein podocin and injury marker desmin in podocytes treated with LG or HG for 48 h with or without transfection of TLR4, MyD88 or scrambled shRNAs. P values were determined by one-way ANOVA and data are presented as mean ± SD. **** P < 0.01; ### P < 0.01; #### P < 0.01 ( n = 3 independent experiments).

    Techniques Used: Activation Assay, In Vivo, In Vitro, Western Blot, Expressing, Staining, Cell Culture, Transfection, Marker

    TLR4/MyD88/PKCδ/SHP-1 signaling cascade induced podocyte damage through regulating ER stress in DKD and podocyte damage. (A) The protein levels of ER stress markers BIP, sXBP-1, ATF4 and ATF6 were determined in renal tissues from mice at 12 and 16 weeks by western blot. P values were determined by one-way ANOVA and data are presented as mean ± SD. **** P < 0.01; # P < 0.05; ## P < 0.01; ### P < 0.01 ( n = 8). (B) Representative double IF staining of the expression ATF4 and podocyte marker synaptopodin in mice at 16 weeks. Scale bar, 20 μm ( n = 8). (C) Representative western blot to assess the levels of ER stress markers BIP, sXBP-1, ATF4 and ATF6 in cultured mouse podocytes from treatments of LG or HG for 48 h with or without transfection of TLR4, MyD88 or scrambled shRNAs. P values were determined by one-way ANOVA and data are presented as mean ± SD. **** P < 0.01; ### P < 0.01; ### P < 0.01 ( n = 3 independent experiments). (D) ER morphological alterations and ER stress illustrated by ER-tracker staining in podocytes treated with LG or HG for 48 h with or without transfection of TLR4, PKCδ, or MCP-1 shRNAs, or pretreatment of 4-PBA. Scale bar, 10 μm. P values were determined by one-way ANOVA and data are presented as mean ± SD. **** P < 0.01; ## P < 0.01; # P < 0.05 ( n = 3 independent experiments). (E) Representative western blot to detect the expression of ER stress proteins BIP, sXBP-1, ATF4 and ATF6 in cultured podocytes after HG stimulation for 48 h with or without transfection of PKCδ, SHP-1 or scrambled shRNAs, or pretreatment of 4-PBA. P values were determined by one-way ANOVA and data are presented as mean ± SD. ** P < 0.01; *** P < 0.01; **** P < 0.01; ## P < 0.01; ### P < 0.01; #### P < 0.01 ( n = 3 independent experiments).
    Figure Legend Snippet: TLR4/MyD88/PKCδ/SHP-1 signaling cascade induced podocyte damage through regulating ER stress in DKD and podocyte damage. (A) The protein levels of ER stress markers BIP, sXBP-1, ATF4 and ATF6 were determined in renal tissues from mice at 12 and 16 weeks by western blot. P values were determined by one-way ANOVA and data are presented as mean ± SD. **** P < 0.01; # P < 0.05; ## P < 0.01; ### P < 0.01 ( n = 8). (B) Representative double IF staining of the expression ATF4 and podocyte marker synaptopodin in mice at 16 weeks. Scale bar, 20 μm ( n = 8). (C) Representative western blot to assess the levels of ER stress markers BIP, sXBP-1, ATF4 and ATF6 in cultured mouse podocytes from treatments of LG or HG for 48 h with or without transfection of TLR4, MyD88 or scrambled shRNAs. P values were determined by one-way ANOVA and data are presented as mean ± SD. **** P < 0.01; ### P < 0.01; ### P < 0.01 ( n = 3 independent experiments). (D) ER morphological alterations and ER stress illustrated by ER-tracker staining in podocytes treated with LG or HG for 48 h with or without transfection of TLR4, PKCδ, or MCP-1 shRNAs, or pretreatment of 4-PBA. Scale bar, 10 μm. P values were determined by one-way ANOVA and data are presented as mean ± SD. **** P < 0.01; ## P < 0.01; # P < 0.05 ( n = 3 independent experiments). (E) Representative western blot to detect the expression of ER stress proteins BIP, sXBP-1, ATF4 and ATF6 in cultured podocytes after HG stimulation for 48 h with or without transfection of PKCδ, SHP-1 or scrambled shRNAs, or pretreatment of 4-PBA. P values were determined by one-way ANOVA and data are presented as mean ± SD. ** P < 0.01; *** P < 0.01; **** P < 0.01; ## P < 0.01; ### P < 0.01; #### P < 0.01 ( n = 3 independent experiments).

    Techniques Used: Western Blot, Staining, Expressing, Marker, Cell Culture, Transfection



    Similar Products

    synaptopodin conjugated to fluorescein  (Progen Biotechnik)
    86
    Progen Biotechnik synaptopodin conjugated to fluorescein
    Synaptopodin Conjugated To Fluorescein, supplied by Progen Biotechnik, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/synaptopodin conjugated to fluorescein/product/Progen Biotechnik
    Average 86 stars, based on 1 article reviews
    synaptopodin conjugated to fluorescein - by Bioz Stars, 2026-06
    86/100 stars
    		  Buy from Supplier
    synaptopodin  (Proteintech)
    94
    Proteintech synaptopodin
    Podocyte-specific TLR4 deletion mitigated STZ-induced DKD, podocyte injury, and proteinuria in mice. (A) Schematic description of the strategy for generating STZ-induced diabetic mouse model in NPHS2 -Cre; TLR4 fl/fl ( TLR4 CKO) mice and in TLR4 fl/fl mice (CTL). (B) Monthly monitoring of plasma glucose levels in mice. Data are presented as mean ± SD. ** P < 0.01 vs. CTL-NC; ## P < 0.01 vs. CKO-NC. (C) Monthly monitoring of body weight in mice. Data are presented as mean ± SD. ** P < 0.01 vs. CTL-NC; # P < 0.05 vs. CKO-NC; # # P < 0.01 vs. CKO-NC; # ### P < 0.01 vs. CTL-STZ. (D) Representative images of PAS and MASSON staining of the glomeruli in mice at 12 and 16 weeks. Scale bar, 20 μm ( n = 8). (E) Representative images of HE staining of the kidney in mice at 12 and 16 weeks. Scale bar, 50 μm ( n = 8). (F) Representative IF images of the expression of SD proteins p-cadherin and <t>synaptopodin</t> in glomeruli at 12 and 16 weeks. Scale bar, 20 μm ( n = 8). (G) The expression of a mesenchymal marker desmin was detected in renal tissues from mice at 12 and 16 weeks by western blot analysis. P values were determined by one-way ANOVA and data are presented as mean ± SD. **** P < 0.01; # P < 0.05; ## P < 0.05 ( n = 8). (H) Representative images of TEM in podocyte ultrastructural alterations in mice. FPs effacement (blue arrow), ER dilation (red arrow) and GBM thickness were observed in podocytes in STZ-induced CTL diabetic mice, and were attenuated in TLR4 CKO diabetic mice at 16 weeks. Scale bar, 1 μm ( n = 8). (I) Determination of proteinuria in mice. Data are presented as mean ± SD. ** P < 0.01 vs. CTL-NC; # P < 0.05 vs. CKO-NC; # # P < 0.01 vs. CKO-NC; # ## P < 0.05 vs. CTL-STZ; # ### P < 0.01 vs. CTL-STZ.
    Synaptopodin, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/synaptopodin/product/Proteintech
    Average 94 stars, based on 1 article reviews
    synaptopodin - by Bioz Stars, 2026-06
    94/100 stars
    		  Buy from Supplier
    rabbit anti synaptopodin antibody  (Proteintech)
    94
    Proteintech rabbit anti synaptopodin antibody
    Podocyte-specific TLR4 deletion mitigated STZ-induced DKD, podocyte injury, and proteinuria in mice. (A) Schematic description of the strategy for generating STZ-induced diabetic mouse model in NPHS2 -Cre; TLR4 fl/fl ( TLR4 CKO) mice and in TLR4 fl/fl mice (CTL). (B) Monthly monitoring of plasma glucose levels in mice. Data are presented as mean ± SD. ** P < 0.01 vs. CTL-NC; ## P < 0.01 vs. CKO-NC. (C) Monthly monitoring of body weight in mice. Data are presented as mean ± SD. ** P < 0.01 vs. CTL-NC; # P < 0.05 vs. CKO-NC; # # P < 0.01 vs. CKO-NC; # ### P < 0.01 vs. CTL-STZ. (D) Representative images of PAS and MASSON staining of the glomeruli in mice at 12 and 16 weeks. Scale bar, 20 μm ( n = 8). (E) Representative images of HE staining of the kidney in mice at 12 and 16 weeks. Scale bar, 50 μm ( n = 8). (F) Representative IF images of the expression of SD proteins p-cadherin and <t>synaptopodin</t> in glomeruli at 12 and 16 weeks. Scale bar, 20 μm ( n = 8). (G) The expression of a mesenchymal marker desmin was detected in renal tissues from mice at 12 and 16 weeks by western blot analysis. P values were determined by one-way ANOVA and data are presented as mean ± SD. **** P < 0.01; # P < 0.05; ## P < 0.05 ( n = 8). (H) Representative images of TEM in podocyte ultrastructural alterations in mice. FPs effacement (blue arrow), ER dilation (red arrow) and GBM thickness were observed in podocytes in STZ-induced CTL diabetic mice, and were attenuated in TLR4 CKO diabetic mice at 16 weeks. Scale bar, 1 μm ( n = 8). (I) Determination of proteinuria in mice. Data are presented as mean ± SD. ** P < 0.01 vs. CTL-NC; # P < 0.05 vs. CKO-NC; # # P < 0.01 vs. CKO-NC; # ## P < 0.05 vs. CTL-STZ; # ### P < 0.01 vs. CTL-STZ.
    Rabbit Anti Synaptopodin Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti synaptopodin antibody/product/Proteintech
    Average 94 stars, based on 1 article reviews
    rabbit anti synaptopodin antibody - by Bioz Stars, 2026-06
    94/100 stars
    		  Buy from Supplier
    anti synaptopodin catalog no sc 515842 antibody  (Santa Cruz Biotechnology)
    95
    Santa Cruz Biotechnology anti synaptopodin catalog no sc 515842 antibody
    Podocyte-specific TLR4 deletion mitigated STZ-induced DKD, podocyte injury, and proteinuria in mice. (A) Schematic description of the strategy for generating STZ-induced diabetic mouse model in NPHS2 -Cre; TLR4 fl/fl ( TLR4 CKO) mice and in TLR4 fl/fl mice (CTL). (B) Monthly monitoring of plasma glucose levels in mice. Data are presented as mean ± SD. ** P < 0.01 vs. CTL-NC; ## P < 0.01 vs. CKO-NC. (C) Monthly monitoring of body weight in mice. Data are presented as mean ± SD. ** P < 0.01 vs. CTL-NC; # P < 0.05 vs. CKO-NC; # # P < 0.01 vs. CKO-NC; # ### P < 0.01 vs. CTL-STZ. (D) Representative images of PAS and MASSON staining of the glomeruli in mice at 12 and 16 weeks. Scale bar, 20 μm ( n = 8). (E) Representative images of HE staining of the kidney in mice at 12 and 16 weeks. Scale bar, 50 μm ( n = 8). (F) Representative IF images of the expression of SD proteins p-cadherin and <t>synaptopodin</t> in glomeruli at 12 and 16 weeks. Scale bar, 20 μm ( n = 8). (G) The expression of a mesenchymal marker desmin was detected in renal tissues from mice at 12 and 16 weeks by western blot analysis. P values were determined by one-way ANOVA and data are presented as mean ± SD. **** P < 0.01; # P < 0.05; ## P < 0.05 ( n = 8). (H) Representative images of TEM in podocyte ultrastructural alterations in mice. FPs effacement (blue arrow), ER dilation (red arrow) and GBM thickness were observed in podocytes in STZ-induced CTL diabetic mice, and were attenuated in TLR4 CKO diabetic mice at 16 weeks. Scale bar, 1 μm ( n = 8). (I) Determination of proteinuria in mice. Data are presented as mean ± SD. ** P < 0.01 vs. CTL-NC; # P < 0.05 vs. CKO-NC; # # P < 0.01 vs. CKO-NC; # ## P < 0.05 vs. CTL-STZ; # ### P < 0.01 vs. CTL-STZ.
    Anti Synaptopodin Catalog No Sc 515842 Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti synaptopodin catalog no sc 515842 antibody/product/Santa Cruz Biotechnology
    Average 95 stars, based on 1 article reviews
    anti synaptopodin catalog no sc 515842 antibody - by Bioz Stars, 2026-06
    95/100 stars
    		  Buy from Supplier
    anti synaptopodin  (Santa Cruz Biotechnology)
    95
    Santa Cruz Biotechnology anti synaptopodin
    Podocyte-specific TLR4 deletion mitigated STZ-induced DKD, podocyte injury, and proteinuria in mice. (A) Schematic description of the strategy for generating STZ-induced diabetic mouse model in NPHS2 -Cre; TLR4 fl/fl ( TLR4 CKO) mice and in TLR4 fl/fl mice (CTL). (B) Monthly monitoring of plasma glucose levels in mice. Data are presented as mean ± SD. ** P < 0.01 vs. CTL-NC; ## P < 0.01 vs. CKO-NC. (C) Monthly monitoring of body weight in mice. Data are presented as mean ± SD. ** P < 0.01 vs. CTL-NC; # P < 0.05 vs. CKO-NC; # # P < 0.01 vs. CKO-NC; # ### P < 0.01 vs. CTL-STZ. (D) Representative images of PAS and MASSON staining of the glomeruli in mice at 12 and 16 weeks. Scale bar, 20 μm ( n = 8). (E) Representative images of HE staining of the kidney in mice at 12 and 16 weeks. Scale bar, 50 μm ( n = 8). (F) Representative IF images of the expression of SD proteins p-cadherin and <t>synaptopodin</t> in glomeruli at 12 and 16 weeks. Scale bar, 20 μm ( n = 8). (G) The expression of a mesenchymal marker desmin was detected in renal tissues from mice at 12 and 16 weeks by western blot analysis. P values were determined by one-way ANOVA and data are presented as mean ± SD. **** P < 0.01; # P < 0.05; ## P < 0.05 ( n = 8). (H) Representative images of TEM in podocyte ultrastructural alterations in mice. FPs effacement (blue arrow), ER dilation (red arrow) and GBM thickness were observed in podocytes in STZ-induced CTL diabetic mice, and were attenuated in TLR4 CKO diabetic mice at 16 weeks. Scale bar, 1 μm ( n = 8). (I) Determination of proteinuria in mice. Data are presented as mean ± SD. ** P < 0.01 vs. CTL-NC; # P < 0.05 vs. CKO-NC; # # P < 0.01 vs. CKO-NC; # ## P < 0.05 vs. CTL-STZ; # ### P < 0.01 vs. CTL-STZ.
    Anti Synaptopodin, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti synaptopodin/product/Santa Cruz Biotechnology
    Average 95 stars, based on 1 article reviews
    anti synaptopodin - by Bioz Stars, 2026-06
    95/100 stars
    		  Buy from Supplier
    synaptopodin  (Santa Cruz Biotechnology)
    95
    Santa Cruz Biotechnology synaptopodin
    Podocyte-specific TLR4 deletion mitigated STZ-induced DKD, podocyte injury, and proteinuria in mice. (A) Schematic description of the strategy for generating STZ-induced diabetic mouse model in NPHS2 -Cre; TLR4 fl/fl ( TLR4 CKO) mice and in TLR4 fl/fl mice (CTL). (B) Monthly monitoring of plasma glucose levels in mice. Data are presented as mean ± SD. ** P < 0.01 vs. CTL-NC; ## P < 0.01 vs. CKO-NC. (C) Monthly monitoring of body weight in mice. Data are presented as mean ± SD. ** P < 0.01 vs. CTL-NC; # P < 0.05 vs. CKO-NC; # # P < 0.01 vs. CKO-NC; # ### P < 0.01 vs. CTL-STZ. (D) Representative images of PAS and MASSON staining of the glomeruli in mice at 12 and 16 weeks. Scale bar, 20 μm ( n = 8). (E) Representative images of HE staining of the kidney in mice at 12 and 16 weeks. Scale bar, 50 μm ( n = 8). (F) Representative IF images of the expression of SD proteins p-cadherin and <t>synaptopodin</t> in glomeruli at 12 and 16 weeks. Scale bar, 20 μm ( n = 8). (G) The expression of a mesenchymal marker desmin was detected in renal tissues from mice at 12 and 16 weeks by western blot analysis. P values were determined by one-way ANOVA and data are presented as mean ± SD. **** P < 0.01; # P < 0.05; ## P < 0.05 ( n = 8). (H) Representative images of TEM in podocyte ultrastructural alterations in mice. FPs effacement (blue arrow), ER dilation (red arrow) and GBM thickness were observed in podocytes in STZ-induced CTL diabetic mice, and were attenuated in TLR4 CKO diabetic mice at 16 weeks. Scale bar, 1 μm ( n = 8). (I) Determination of proteinuria in mice. Data are presented as mean ± SD. ** P < 0.01 vs. CTL-NC; # P < 0.05 vs. CKO-NC; # # P < 0.01 vs. CKO-NC; # ## P < 0.05 vs. CTL-STZ; # ### P < 0.01 vs. CTL-STZ.
    Synaptopodin, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/synaptopodin/product/Santa Cruz Biotechnology
    Average 95 stars, based on 1 article reviews
    synaptopodin - by Bioz Stars, 2026-06
    95/100 stars
    		  Buy from Supplier
    anti synaptopodin  (Proteintech)
    97
    Proteintech anti synaptopodin
    Podocyte-specific TLR4 deletion mitigated STZ-induced DKD, podocyte injury, and proteinuria in mice. (A) Schematic description of the strategy for generating STZ-induced diabetic mouse model in NPHS2 -Cre; TLR4 fl/fl ( TLR4 CKO) mice and in TLR4 fl/fl mice (CTL). (B) Monthly monitoring of plasma glucose levels in mice. Data are presented as mean ± SD. ** P < 0.01 vs. CTL-NC; ## P < 0.01 vs. CKO-NC. (C) Monthly monitoring of body weight in mice. Data are presented as mean ± SD. ** P < 0.01 vs. CTL-NC; # P < 0.05 vs. CKO-NC; # # P < 0.01 vs. CKO-NC; # ### P < 0.01 vs. CTL-STZ. (D) Representative images of PAS and MASSON staining of the glomeruli in mice at 12 and 16 weeks. Scale bar, 20 μm ( n = 8). (E) Representative images of HE staining of the kidney in mice at 12 and 16 weeks. Scale bar, 50 μm ( n = 8). (F) Representative IF images of the expression of SD proteins p-cadherin and <t>synaptopodin</t> in glomeruli at 12 and 16 weeks. Scale bar, 20 μm ( n = 8). (G) The expression of a mesenchymal marker desmin was detected in renal tissues from mice at 12 and 16 weeks by western blot analysis. P values were determined by one-way ANOVA and data are presented as mean ± SD. **** P < 0.01; # P < 0.05; ## P < 0.05 ( n = 8). (H) Representative images of TEM in podocyte ultrastructural alterations in mice. FPs effacement (blue arrow), ER dilation (red arrow) and GBM thickness were observed in podocytes in STZ-induced CTL diabetic mice, and were attenuated in TLR4 CKO diabetic mice at 16 weeks. Scale bar, 1 μm ( n = 8). (I) Determination of proteinuria in mice. Data are presented as mean ± SD. ** P < 0.01 vs. CTL-NC; # P < 0.05 vs. CKO-NC; # # P < 0.01 vs. CKO-NC; # ## P < 0.05 vs. CTL-STZ; # ### P < 0.01 vs. CTL-STZ.
    Anti Synaptopodin, supplied by Proteintech, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti synaptopodin/product/Proteintech
    Average 97 stars, based on 1 article reviews
    anti synaptopodin - by Bioz Stars, 2026-06
    97/100 stars
    		  Buy from Supplier

    Image Search Results


    Podocyte-specific TLR4 deletion mitigated STZ-induced DKD, podocyte injury, and proteinuria in mice. (A) Schematic description of the strategy for generating STZ-induced diabetic mouse model in NPHS2 -Cre; TLR4 fl/fl ( TLR4 CKO) mice and in TLR4 fl/fl mice (CTL). (B) Monthly monitoring of plasma glucose levels in mice. Data are presented as mean ± SD. ** P < 0.01 vs. CTL-NC; ## P < 0.01 vs. CKO-NC. (C) Monthly monitoring of body weight in mice. Data are presented as mean ± SD. ** P < 0.01 vs. CTL-NC; # P < 0.05 vs. CKO-NC; # # P < 0.01 vs. CKO-NC; # ### P < 0.01 vs. CTL-STZ. (D) Representative images of PAS and MASSON staining of the glomeruli in mice at 12 and 16 weeks. Scale bar, 20 μm ( n = 8). (E) Representative images of HE staining of the kidney in mice at 12 and 16 weeks. Scale bar, 50 μm ( n = 8). (F) Representative IF images of the expression of SD proteins p-cadherin and synaptopodin in glomeruli at 12 and 16 weeks. Scale bar, 20 μm ( n = 8). (G) The expression of a mesenchymal marker desmin was detected in renal tissues from mice at 12 and 16 weeks by western blot analysis. P values were determined by one-way ANOVA and data are presented as mean ± SD. **** P < 0.01; # P < 0.05; ## P < 0.05 ( n = 8). (H) Representative images of TEM in podocyte ultrastructural alterations in mice. FPs effacement (blue arrow), ER dilation (red arrow) and GBM thickness were observed in podocytes in STZ-induced CTL diabetic mice, and were attenuated in TLR4 CKO diabetic mice at 16 weeks. Scale bar, 1 μm ( n = 8). (I) Determination of proteinuria in mice. Data are presented as mean ± SD. ** P < 0.01 vs. CTL-NC; # P < 0.05 vs. CKO-NC; # # P < 0.01 vs. CKO-NC; # ## P < 0.05 vs. CTL-STZ; # ### P < 0.01 vs. CTL-STZ.

    Journal: Journal of Advanced Research

    Article Title: Podocyte TLR4 deletion alleviates diabetic kidney disease through prohibiting PKCδ/SHP-1-dependent ER stress and relieving podocyte damage and inflammation

    doi: 10.1016/j.jare.2025.07.013

    Figure Lengend Snippet: Podocyte-specific TLR4 deletion mitigated STZ-induced DKD, podocyte injury, and proteinuria in mice. (A) Schematic description of the strategy for generating STZ-induced diabetic mouse model in NPHS2 -Cre; TLR4 fl/fl ( TLR4 CKO) mice and in TLR4 fl/fl mice (CTL). (B) Monthly monitoring of plasma glucose levels in mice. Data are presented as mean ± SD. ** P < 0.01 vs. CTL-NC; ## P < 0.01 vs. CKO-NC. (C) Monthly monitoring of body weight in mice. Data are presented as mean ± SD. ** P < 0.01 vs. CTL-NC; # P < 0.05 vs. CKO-NC; # # P < 0.01 vs. CKO-NC; # ### P < 0.01 vs. CTL-STZ. (D) Representative images of PAS and MASSON staining of the glomeruli in mice at 12 and 16 weeks. Scale bar, 20 μm ( n = 8). (E) Representative images of HE staining of the kidney in mice at 12 and 16 weeks. Scale bar, 50 μm ( n = 8). (F) Representative IF images of the expression of SD proteins p-cadherin and synaptopodin in glomeruli at 12 and 16 weeks. Scale bar, 20 μm ( n = 8). (G) The expression of a mesenchymal marker desmin was detected in renal tissues from mice at 12 and 16 weeks by western blot analysis. P values were determined by one-way ANOVA and data are presented as mean ± SD. **** P < 0.01; # P < 0.05; ## P < 0.05 ( n = 8). (H) Representative images of TEM in podocyte ultrastructural alterations in mice. FPs effacement (blue arrow), ER dilation (red arrow) and GBM thickness were observed in podocytes in STZ-induced CTL diabetic mice, and were attenuated in TLR4 CKO diabetic mice at 16 weeks. Scale bar, 1 μm ( n = 8). (I) Determination of proteinuria in mice. Data are presented as mean ± SD. ** P < 0.01 vs. CTL-NC; # P < 0.05 vs. CKO-NC; # # P < 0.01 vs. CKO-NC; # ## P < 0.05 vs. CTL-STZ; # ### P < 0.01 vs. CTL-STZ.

    Article Snippet: The following primary antibodies were used: f4/80 (29414–1-AP), desmin (16520–1-AP), MCP-1 (26161–1-AP), podocin (20384–1-AP), synaptopodin (21064–1-AP), TLR4 (66350–1-Ig), β-actin (66009–1-Ig), PKCδ(14188–1-AP), ATF6 (24169–1-AP), p-cadherin (13773–1-AP) were from Proteintech (IL, USA); phospho-PKCδ (Y311) (ab76181), PKCdelta (ab182126), SHP-1 (ab227503), HA (ab9110) were from Abcam (MA, USA); BIP (M010300), MyD88 (P012343) were from Epizyme, Shanghai, China); and ATF4 (11815), sXBP-1 (40435) were from Cell Signaling Technology (MA, USA).

    Techniques: Clinical Proteomics, Staining, Expressing, Marker, Western Blot

    Activation of TLR4/MyD88/PKCδ/SHP-1 signaling cascade was involved in hyperglycemic podocyte damage in vivo and in vitro . (A) The protein levels of TLR4 were determined in renal tissues from mice at 12 and 16 weeks by western blot analysis. P values were determined by one-way ANOVA and data are presented as mean ± SD. **** P < 0.01; # P < 0.05 ( n = 8). (B) Representative western blot of the expression of MyD88, p-PKCδ (Y311), PKCδ and SHP-1 in renal cortex from mice at 12 and 16 weeks. P values were determined by one-way ANOVA and data are presented as mean ± SD. **** P < 0.01; # P < 0.05; ## P < 0.01; ### P < 0.01 ( n = 8). (C) Representative double IF staining of glomerular SHP-1 and synaptopodin expression in mice at 16 weeks. Scale bar, 20 μm ( n = 8). (D) The expression of TLR4, MyD88, p-PKCδ, PKCδ and SHP-1 in cultured mouse podocytes from treatments of LG or HG for 48 h with or without transfection of TLR4, MyD88 or scrambled shRNAs. P values were determined by one-way ANOVA and data are presented as mean ± SD. **** P < 0.01; ## P < 0.01; ### P < 0.01; #### P < 0.01 ( n = 3 independent experiments). (E) IF staining of podocyte proteins p-cadherin and synaptopodin after LG or HG stimulation for 48 h with or without transfection of TLR4, PKCδ, MCP-1 or scrambled shRNAs, or pretreatment of 4-PBA. Magnification 400 × . ( n = 3 independent experiments). (F) Determination of SD protein podocin and injury marker desmin in podocytes treated with LG or HG for 48 h with or without transfection of TLR4, MyD88 or scrambled shRNAs. P values were determined by one-way ANOVA and data are presented as mean ± SD. **** P < 0.01; ### P < 0.01; #### P < 0.01 ( n = 3 independent experiments).

    Journal: Journal of Advanced Research

    Article Title: Podocyte TLR4 deletion alleviates diabetic kidney disease through prohibiting PKCδ/SHP-1-dependent ER stress and relieving podocyte damage and inflammation

    doi: 10.1016/j.jare.2025.07.013

    Figure Lengend Snippet: Activation of TLR4/MyD88/PKCδ/SHP-1 signaling cascade was involved in hyperglycemic podocyte damage in vivo and in vitro . (A) The protein levels of TLR4 were determined in renal tissues from mice at 12 and 16 weeks by western blot analysis. P values were determined by one-way ANOVA and data are presented as mean ± SD. **** P < 0.01; # P < 0.05 ( n = 8). (B) Representative western blot of the expression of MyD88, p-PKCδ (Y311), PKCδ and SHP-1 in renal cortex from mice at 12 and 16 weeks. P values were determined by one-way ANOVA and data are presented as mean ± SD. **** P < 0.01; # P < 0.05; ## P < 0.01; ### P < 0.01 ( n = 8). (C) Representative double IF staining of glomerular SHP-1 and synaptopodin expression in mice at 16 weeks. Scale bar, 20 μm ( n = 8). (D) The expression of TLR4, MyD88, p-PKCδ, PKCδ and SHP-1 in cultured mouse podocytes from treatments of LG or HG for 48 h with or without transfection of TLR4, MyD88 or scrambled shRNAs. P values were determined by one-way ANOVA and data are presented as mean ± SD. **** P < 0.01; ## P < 0.01; ### P < 0.01; #### P < 0.01 ( n = 3 independent experiments). (E) IF staining of podocyte proteins p-cadherin and synaptopodin after LG or HG stimulation for 48 h with or without transfection of TLR4, PKCδ, MCP-1 or scrambled shRNAs, or pretreatment of 4-PBA. Magnification 400 × . ( n = 3 independent experiments). (F) Determination of SD protein podocin and injury marker desmin in podocytes treated with LG or HG for 48 h with or without transfection of TLR4, MyD88 or scrambled shRNAs. P values were determined by one-way ANOVA and data are presented as mean ± SD. **** P < 0.01; ### P < 0.01; #### P < 0.01 ( n = 3 independent experiments).

    Article Snippet: The following primary antibodies were used: f4/80 (29414–1-AP), desmin (16520–1-AP), MCP-1 (26161–1-AP), podocin (20384–1-AP), synaptopodin (21064–1-AP), TLR4 (66350–1-Ig), β-actin (66009–1-Ig), PKCδ(14188–1-AP), ATF6 (24169–1-AP), p-cadherin (13773–1-AP) were from Proteintech (IL, USA); phospho-PKCδ (Y311) (ab76181), PKCdelta (ab182126), SHP-1 (ab227503), HA (ab9110) were from Abcam (MA, USA); BIP (M010300), MyD88 (P012343) were from Epizyme, Shanghai, China); and ATF4 (11815), sXBP-1 (40435) were from Cell Signaling Technology (MA, USA).

    Techniques: Activation Assay, In Vivo, In Vitro, Western Blot, Expressing, Staining, Cell Culture, Transfection, Marker

    TLR4/MyD88/PKCδ/SHP-1 signaling cascade induced podocyte damage through regulating ER stress in DKD and podocyte damage. (A) The protein levels of ER stress markers BIP, sXBP-1, ATF4 and ATF6 were determined in renal tissues from mice at 12 and 16 weeks by western blot. P values were determined by one-way ANOVA and data are presented as mean ± SD. **** P < 0.01; # P < 0.05; ## P < 0.01; ### P < 0.01 ( n = 8). (B) Representative double IF staining of the expression ATF4 and podocyte marker synaptopodin in mice at 16 weeks. Scale bar, 20 μm ( n = 8). (C) Representative western blot to assess the levels of ER stress markers BIP, sXBP-1, ATF4 and ATF6 in cultured mouse podocytes from treatments of LG or HG for 48 h with or without transfection of TLR4, MyD88 or scrambled shRNAs. P values were determined by one-way ANOVA and data are presented as mean ± SD. **** P < 0.01; ### P < 0.01; ### P < 0.01 ( n = 3 independent experiments). (D) ER morphological alterations and ER stress illustrated by ER-tracker staining in podocytes treated with LG or HG for 48 h with or without transfection of TLR4, PKCδ, or MCP-1 shRNAs, or pretreatment of 4-PBA. Scale bar, 10 μm. P values were determined by one-way ANOVA and data are presented as mean ± SD. **** P < 0.01; ## P < 0.01; # P < 0.05 ( n = 3 independent experiments). (E) Representative western blot to detect the expression of ER stress proteins BIP, sXBP-1, ATF4 and ATF6 in cultured podocytes after HG stimulation for 48 h with or without transfection of PKCδ, SHP-1 or scrambled shRNAs, or pretreatment of 4-PBA. P values were determined by one-way ANOVA and data are presented as mean ± SD. ** P < 0.01; *** P < 0.01; **** P < 0.01; ## P < 0.01; ### P < 0.01; #### P < 0.01 ( n = 3 independent experiments).

    Journal: Journal of Advanced Research

    Article Title: Podocyte TLR4 deletion alleviates diabetic kidney disease through prohibiting PKCδ/SHP-1-dependent ER stress and relieving podocyte damage and inflammation

    doi: 10.1016/j.jare.2025.07.013

    Figure Lengend Snippet: TLR4/MyD88/PKCδ/SHP-1 signaling cascade induced podocyte damage through regulating ER stress in DKD and podocyte damage. (A) The protein levels of ER stress markers BIP, sXBP-1, ATF4 and ATF6 were determined in renal tissues from mice at 12 and 16 weeks by western blot. P values were determined by one-way ANOVA and data are presented as mean ± SD. **** P < 0.01; # P < 0.05; ## P < 0.01; ### P < 0.01 ( n = 8). (B) Representative double IF staining of the expression ATF4 and podocyte marker synaptopodin in mice at 16 weeks. Scale bar, 20 μm ( n = 8). (C) Representative western blot to assess the levels of ER stress markers BIP, sXBP-1, ATF4 and ATF6 in cultured mouse podocytes from treatments of LG or HG for 48 h with or without transfection of TLR4, MyD88 or scrambled shRNAs. P values were determined by one-way ANOVA and data are presented as mean ± SD. **** P < 0.01; ### P < 0.01; ### P < 0.01 ( n = 3 independent experiments). (D) ER morphological alterations and ER stress illustrated by ER-tracker staining in podocytes treated with LG or HG for 48 h with or without transfection of TLR4, PKCδ, or MCP-1 shRNAs, or pretreatment of 4-PBA. Scale bar, 10 μm. P values were determined by one-way ANOVA and data are presented as mean ± SD. **** P < 0.01; ## P < 0.01; # P < 0.05 ( n = 3 independent experiments). (E) Representative western blot to detect the expression of ER stress proteins BIP, sXBP-1, ATF4 and ATF6 in cultured podocytes after HG stimulation for 48 h with or without transfection of PKCδ, SHP-1 or scrambled shRNAs, or pretreatment of 4-PBA. P values were determined by one-way ANOVA and data are presented as mean ± SD. ** P < 0.01; *** P < 0.01; **** P < 0.01; ## P < 0.01; ### P < 0.01; #### P < 0.01 ( n = 3 independent experiments).

    Article Snippet: The following primary antibodies were used: f4/80 (29414–1-AP), desmin (16520–1-AP), MCP-1 (26161–1-AP), podocin (20384–1-AP), synaptopodin (21064–1-AP), TLR4 (66350–1-Ig), β-actin (66009–1-Ig), PKCδ(14188–1-AP), ATF6 (24169–1-AP), p-cadherin (13773–1-AP) were from Proteintech (IL, USA); phospho-PKCδ (Y311) (ab76181), PKCdelta (ab182126), SHP-1 (ab227503), HA (ab9110) were from Abcam (MA, USA); BIP (M010300), MyD88 (P012343) were from Epizyme, Shanghai, China); and ATF4 (11815), sXBP-1 (40435) were from Cell Signaling Technology (MA, USA).

    Techniques: Western Blot, Staining, Expressing, Marker, Cell Culture, Transfection