nbp2 (Novus Biologicals)
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Novus Biologicals
nbp2
Nbp2, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/synaptophysin/Synaptophysin+Antibody+-+BSA+Free/pmc12993000-12-7-2
Average 94 stars, based on 9 article reviews
Nbp2, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/synaptophysin/Synaptophysin+Antibody+-+BSA+Free/pmc12993000-12-7-2
Average 94 stars, based on 9 article reviews
nbp2 - by Bioz Stars,
2026-09
94/100 stars
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Incubation:Article Title: Deficiency of <scp>IKKβ</scp> in neurons ameliorates Alzheimer's disease pathology in <scp>APP</scp> ‐ and tau‐transgenic mice Article Snippet: Protein Assay (Bio- Rad Laboratories GmbH, München, Germany), the protein samples were separated through 10% or 12% SDS- PAGE gels. .. Proteins were then transferred onto polyvinylidene difluoride (PVDF) or nitrocellulose membranes and incubated overnight at 4°C with the following antibodies: mouse monoclonal antibody against Aβ (clone W0- 2; Sigma- Aldrich); rabbit monoclonal antibodies against beclin1 (clone D40C5), phosphor- glycogen synthase kinase (GSK)- 3β (clone D3A4), GSK- 3β (clone 27C10), phospho- CREB (Ser133) (clone 87G3), CREB (clone 48H2), cleaved Caspase- 3 (clone 5A1E), PSD- 95 (clone D27E11), Article Title: Combination antiviral and anti-inflammatory therapy mitigates persistent neurological deficits in mice post SARS-CoV-2 infection Article Snippet: .. Sections were then rinsed in PBS followed by incubation with CAS block (Invitrogen, Thermo Fisher Scientific) for 60 min. Primary antibodies against Iba-1 (Wako, 1:1,000), synapsin (Cell signaling, 1:100), Article Title: Oral Administration of Astrocyte-Targeted Natural Antioxidants Suppress NOX4-Driven Neuroinflammation and Restore Hippocampal Neurogenesis in MPTP-Induced Parkinson’s Disease Mouse Model Article Snippet: .. Immunofluorescence (IF): Sections underwent citrate buffer antigen retrieval and blocking before incubation with primary antibodies (overnight, 4 °C): BrdU (1:500, Cell Signaling, Boston, MA, USA), Glial Fibrillary Acidic Protein (GFAP; 1:300, Millipore, Burlington, MA, USA), MPO (1:100, R&D Systems, Minneapolis, MN, USA), Neuronal Nuclei (NeuN; 1:500, Abcam), NOX4 (1:100, Santa Cruz), OPN (1:500, R&D Systems), Postsynaptic Density Protein-95 (PSD95; 1:500, Invitrogen, Waltham, MA, USA), and Bioprocessing:Article Title: Deficiency of <scp>IKKβ</scp> in neurons ameliorates Alzheimer's disease pathology in <scp>APP</scp> ‐ and tau‐transgenic mice Article Snippet: Protein Assay (Bio- Rad Laboratories GmbH, München, Germany), the protein samples were separated through 10% or 12% SDS- PAGE gels. .. Proteins were then transferred onto polyvinylidene difluoride (PVDF) or nitrocellulose membranes and incubated overnight at 4°C with the following antibodies: mouse monoclonal antibody against Aβ (clone W0- 2; Sigma- Aldrich); rabbit monoclonal antibodies against beclin1 (clone D40C5), phosphor- glycogen synthase kinase (GSK)- 3β (clone D3A4), GSK- 3β (clone 27C10), phospho- CREB (Ser133) (clone 87G3), CREB (clone 48H2), cleaved Caspase- 3 (clone 5A1E), PSD- 95 (clone D27E11), Purification:Article Title: Loss of spines in the prelimbic cortex is detrimental to working memory in mice with early-life adversity Article Snippet: .. In brief, the primary antibody was pre-adsorbed with a purified PSD-95 (5 μg/ml, Novus Biologicals, CO, USA) or Article Title: Loss of spines in the prelimbic cortex is detrimental to working memory in mice with early-life adversity. Article Snippet: .. In brief, the primary antibody was pre-adsorbed with a purified PSD-95 (5 μg/ml, Novus Biologicals, CO, USA) or Recombinant:Article Title: Loss of spines in the prelimbic cortex is detrimental to working memory in mice with early-life adversity Article Snippet: .. In brief, the primary antibody was pre-adsorbed with a purified PSD-95 (5 μg/ml, Novus Biologicals, CO, USA) or Article Title: Loss of spines in the prelimbic cortex is detrimental to working memory in mice with early-life adversity. Article Snippet: .. In brief, the primary antibody was pre-adsorbed with a purified PSD-95 (5 μg/ml, Novus Biologicals, CO, USA) or Labeling:Article Title: Loss of spines in the prelimbic cortex is detrimental to working memory in mice with early-life adversity Article Snippet: .. In brief, the primary antibody was pre-adsorbed with a purified PSD-95 (5 μg/ml, Novus Biologicals, CO, USA) or Article Title: Loss of spines in the prelimbic cortex is detrimental to working memory in mice with early-life adversity. Article Snippet: .. In brief, the primary antibody was pre-adsorbed with a purified PSD-95 (5 μg/ml, Novus Biologicals, CO, USA) or Blocking Assay:Article Title: Combination antiviral and anti-inflammatory therapy mitigates persistent neurological deficits in mice post SARS-CoV-2 infection Article Snippet: .. Sections were then rinsed in PBS followed by incubation with CAS block (Invitrogen, Thermo Fisher Scientific) for 60 min. Primary antibodies against Iba-1 (Wako, 1:1,000), synapsin (Cell signaling, 1:100), Article Title: Oral Administration of Astrocyte-Targeted Natural Antioxidants Suppress NOX4-Driven Neuroinflammation and Restore Hippocampal Neurogenesis in MPTP-Induced Parkinson’s Disease Mouse Model Article Snippet: .. Immunofluorescence (IF): Sections underwent citrate buffer antigen retrieval and blocking before incubation with primary antibodies (overnight, 4 °C): BrdU (1:500, Cell Signaling, Boston, MA, USA), Glial Fibrillary Acidic Protein (GFAP; 1:300, Millipore, Burlington, MA, USA), MPO (1:100, R&D Systems, Minneapolis, MN, USA), Neuronal Nuclei (NeuN; 1:500, Abcam), NOX4 (1:100, Santa Cruz), OPN (1:500, R&D Systems), Postsynaptic Density Protein-95 (PSD95; 1:500, Invitrogen, Waltham, MA, USA), and Immunohistochemical staining:Article Title: Shotgun Proteomic-Based Approach with a Q-Exactive Hybrid Quadrupole-Orbitrap High-Resolution Mass Spectrometer for Protein Adductomics on a 3D Human Brain Tumor Neurospheroid Culture Model: The Identification of Adduct Formation in Calmodulin-Dependent Protein Kinase-2 and Annexin-A1 Induced by Pesticide Mixture. Article Snippet: We manually inspected neurosphere growth under an inverted phase Contrast microscope (MOTIC AE21, Motic Incorporation Ltd. Hong Kong, China), equipped with an Axiocam camera 208 [Sony CMOS image sensor color, rolling shutter; square pixels of 1.85 μM side length; 3840 × 2160 pixel resolution; ultra HD (4K); 3 × 8 bits/pixel] (Zeiss, France) at scale bars of 100 μm, 150, and 200 μm before proceeding. .. Primary cell identity was confirmed prior to use by immunohistochemical (IHC) staining for neuronal differentiation markers such as NSE (neuron-specific enolase) (IMGENEX, Cat# IMG-80398, RRID:AB_1151008), chromogranin (Fitzgerald Industries International Cat# 10RC126ax, RRID:AB_1283819), Immunohistochemistry:Article Title: Shotgun Proteomic-Based Approach with a Q-Exactive Hybrid Quadrupole-Orbitrap High-Resolution Mass Spectrometer for Protein Adductomics on a 3D Human Brain Tumor Neurospheroid Culture Model: The Identification of Adduct Formation in Calmodulin-Dependent Protein Kinase-2 and Annexin-A1 Induced by Pesticide Mixture. Article Snippet: We manually inspected neurosphere growth under an inverted phase Contrast microscope (MOTIC AE21, Motic Incorporation Ltd. Hong Kong, China), equipped with an Axiocam camera 208 [Sony CMOS image sensor color, rolling shutter; square pixels of 1.85 μM side length; 3840 × 2160 pixel resolution; ultra HD (4K); 3 × 8 bits/pixel] (Zeiss, France) at scale bars of 100 μm, 150, and 200 μm before proceeding. .. Primary cell identity was confirmed prior to use by immunohistochemical (IHC) staining for neuronal differentiation markers such as NSE (neuron-specific enolase) (IMGENEX, Cat# IMG-80398, RRID:AB_1151008), chromogranin (Fitzgerald Industries International Cat# 10RC126ax, RRID:AB_1283819), Staining:Article Title: Shotgun Proteomic-Based Approach with a Q-Exactive Hybrid Quadrupole-Orbitrap High-Resolution Mass Spectrometer for Protein Adductomics on a 3D Human Brain Tumor Neurospheroid Culture Model: The Identification of Adduct Formation in Calmodulin-Dependent Protein Kinase-2 and Annexin-A1 Induced by Pesticide Mixture. Article Snippet: We manually inspected neurosphere growth under an inverted phase Contrast microscope (MOTIC AE21, Motic Incorporation Ltd. Hong Kong, China), equipped with an Axiocam camera 208 [Sony CMOS image sensor color, rolling shutter; square pixels of 1.85 μM side length; 3840 × 2160 pixel resolution; ultra HD (4K); 3 × 8 bits/pixel] (Zeiss, France) at scale bars of 100 μm, 150, and 200 μm before proceeding. .. Primary cell identity was confirmed prior to use by immunohistochemical (IHC) staining for neuronal differentiation markers such as NSE (neuron-specific enolase) (IMGENEX, Cat# IMG-80398, RRID:AB_1151008), chromogranin (Fitzgerald Industries International Cat# 10RC126ax, RRID:AB_1283819), Marker:Article Title: Shotgun Proteomic-Based Approach with a Q-Exactive Hybrid Quadrupole-Orbitrap High-Resolution Mass Spectrometer for Protein Adductomics on a 3D Human Brain Tumor Neurospheroid Culture Model: The Identification of Adduct Formation in Calmodulin-Dependent Protein Kinase-2 and Annexin-A1 Induced by Pesticide Mixture. Article Snippet: We manually inspected neurosphere growth under an inverted phase Contrast microscope (MOTIC AE21, Motic Incorporation Ltd. Hong Kong, China), equipped with an Axiocam camera 208 [Sony CMOS image sensor color, rolling shutter; square pixels of 1.85 μM side length; 3840 × 2160 pixel resolution; ultra HD (4K); 3 × 8 bits/pixel] (Zeiss, France) at scale bars of 100 μm, 150, and 200 μm before proceeding. .. Primary cell identity was confirmed prior to use by immunohistochemical (IHC) staining for neuronal differentiation markers such as NSE (neuron-specific enolase) (IMGENEX, Cat# IMG-80398, RRID:AB_1151008), chromogranin (Fitzgerald Industries International Cat# 10RC126ax, RRID:AB_1283819), Immunofluorescence:Article Title: Oral Administration of Astrocyte-Targeted Natural Antioxidants Suppress NOX4-Driven Neuroinflammation and Restore Hippocampal Neurogenesis in MPTP-Induced Parkinson’s Disease Mouse Model Article Snippet: .. Immunofluorescence (IF): Sections underwent citrate buffer antigen retrieval and blocking before incubation with primary antibodies (overnight, 4 °C): BrdU (1:500, Cell Signaling, Boston, MA, USA), Glial Fibrillary Acidic Protein (GFAP; 1:300, Millipore, Burlington, MA, USA), MPO (1:100, R&D Systems, Minneapolis, MN, USA), Neuronal Nuclei (NeuN; 1:500, Abcam), NOX4 (1:100, Santa Cruz), OPN (1:500, R&D Systems), Postsynaptic Density Protein-95 (PSD95; 1:500, Invitrogen, Waltham, MA, USA), and other:Article Title: Anti-Inflammatory and Synaptic Protective Effects of TNF-α Inactivation in the MDX Mouse Model Article Snippet: |
