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Image Search Results
Journal: Cells
Article Title: Characterization and Biomarker Analyses of Post-COVID-19 Complications and Neurological Manifestations
doi: 10.3390/cells10020386
Figure Lengend Snippet: nEV characterization. Representative NTA spectra from Control ( A ), CoV ( B ), and nCoV ( C ) showing the heterogeneous nature of the nEVs from COVID-19 participants. Variance of sizes were significantly different among the groups, Levene test p < 0.0001. nEV sizes determined by NTA ( D ) for Control ( n = 12), CoV ( n = 16), and nCoV ( n = 8) showed no difference between the groups. nEV concentrations were determined by NTA ( E ) ( n is the same as panel D). nEV protein cargo for CD81 ( F ), ALIX ( G ), and SYP ( H ) were determined using ELISA. CD81 showed an increase in nCoV ( n = 8) compared to Control ( n = 6). ALIX increased in nCoV ( n = 8) compared to Control ( n = 12) but not significantly to CoV ( n = 16). (H) Synaptophysin (SYP) is enriched in nEV ( n = 30) compared to total EVs (all EVs in plasma, n = 30). Kruskal–Wallis test was used and followed with post hoc Dunn tests if significant for groupwise differences.
Article Snippet: ELISA was performed on the nEV lysates to determine protein concentrations for CD81, Programmed Cell Death 6 Interacting Protein (PDCD6IP, also known as ALIX), Synaptophysin (SYP), and High Mobility Group Box 1 (HMGB1) using commercially-available kits as follows: CD81 (American Research Products, Inc., Waltham, MA, USA; catalog # CSB-EL004960HU, detection range: 0.156–10 ng/mL), ALIX (Lifeome, Oceanside, CA, USA; catalog #CSB-EL017673HU, range: 47–3000 pg/mL),
Techniques: Control, Enzyme-linked Immunosorbent Assay, Clinical Proteomics
Journal: Acta neuropathologica
Article Title: Brain regional correlation of amyloid-? with synapses and apolipoprotein E in non-demented individuals: potential mechanisms underlying regional vulnerability to amyloid-? accumulation
doi: 10.1007/s00401-013-1086-9
Figure Lengend Snippet: Regional distribution of molecules related with Aβ metabolism. After normalization within each individual, levels of apoE in TBS fraction (a) and in TBS-TX fraction (b), APP (c), APP-CTFβ (d), BACE1 (e), presenilin-1 (f), NEP (g), IDE (h), LRP1 (i), LDLR (j), synaptophysin (k), PSD95 (l), lactate (m), and GFAP (n) are plotted by different brain areas with the average line of each area. The p < 0.05 was considered significant; paired t test with Holm correction for multiple comparisons. PS1 presenilin-1, SYP synaptophysin
Article Snippet: The recombinant
Techniques: